Restriction map for
pFN21AB4028
(Circular) (Six-base) MAPSORT of: pFN21AB4028.seq Check: 5443 from: 1 to: 6388
pFN21AB4028 6388 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4198 278
Size: 53 83 186 936 2662 2468
Fragments arranged by size:
2662 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3731 1937
Size: 1794 4594
Acc65I G'GTAC_C
Cuts at: 3709 3709
Size: 6388
AclI AA'CG_TT
Cuts at: 1563 2740 4515 4888 1563
Size: 1177 1775 373 3063
Fragments arranged by size:
3063 1775 1177 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3012 3397 3414 4451 5835 6103 1632
Size: 1380 385 17 1037 1384 268 1917
Fragments arranged by size:
1917 1384 1380 1037 385 268 17
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6191
AflIII A'CryG_T
Cuts at: 1203 5287 6264 1203
Size: 4084 977 1327
Fragments arranged by size:
4084 1327 977
AgeI A'CCGG_T
Cuts at: 2148 5219 2148
Size: 3071 3317
AhdI GACnn_n'nnGTC
Cuts at: 5117 5117
Size: 6388
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6388
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6388
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3240 3272 3240
Size: 32 6356
AlwNI CAG_nnn'CTG
Cuts at: 2059 2974 4131 5703 2059
Size: 915 1157 1572 2744
Fragments arranged by size:
2744 1572 1157 915
ApaLI G'TGCA_C
Cuts at: 4445 5601 4445
Size: 1156 5232
ApoI r'AATT_y
Cuts at: 1493 2270 3315 3439 3515 3697 3923 4075 6184
Size: 777 1045 124 76 182 226 152 2109
Cuts at: 6184 1493
Size: 1697
Fragments arranged by size:
2109 1697 1045 777 226 182 152 124
76
AseI AT'TA_AT
Cuts at: 160 1032 4942 160
Size: 872 3910 1606
Fragments arranged by size:
3910 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6388
AvaI C'yCGr_G
Cuts at: 1870 1943 3713 1870
Size: 73 1770 4545
Fragments arranged by size:
4545 1770 73
BamHI G'GATC_C
Cuts at: 2712 3718 4085 2712
Size: 1006 367 5015
Fragments arranged by size:
5015 1006 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3709 5164 618
Size: 325 217 627 1922 1455 1842
Fragments arranged by size:
1922 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3707 1391
Size: 2316 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2883 928
Size: 971 984 4433
Fragments arranged by size:
4433 984 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2171 2205 4579 4613 1903
Size: 34 234 34 2374 34 3678
Fragments arranged by size:
3678 2374 234 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2114 2377 2856 4280 5490 2114
Size: 263 479 1424 1210 3012
Fragments arranged by size:
3012 1424 1210 479 263
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6388
BfrBI ATG'CAT
Cuts at: 2096 2096
Size: 6388
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4999 136
Size: 107 122 71 631 605 3327 1525
Fragments arranged by size:
3327 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6383 6383
Size: 6388
BlpI GC'TnA_GC
Cuts at: 3602 3805 3602
Size: 203 6185
Bme1580I G_kGCm'C
Cuts at: 1790 4449 5605 1790
Size: 2659 1156 2573
Fragments arranged by size:
2659 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6388
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4119 5077 442
Size: 416 53 83 399 24 2702 958 1753
Fragments arranged by size:
2702 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6388
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2776 2837 3114 5048 1239
Size: 1537 61 277 1934 2579
Fragments arranged by size:
2579 1934 1537 277 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4513 5378 5676 4513
Size: 865 298 5225
Fragments arranged by size:
5225 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5051 882
Size: 617 3552 2219
Fragments arranged by size:
3552 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4179 493
Size: 711 2975 2702
Fragments arranged by size:
2975 2702 711
BsaBI GATnn'nnATC
Cuts at: 3369 6382 3369
Size: 3013 3375
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4195 4577 5212 275
Size: 53 83 186 936 2662 382 635 1451
Fragments arranged by size:
2662 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2148 4821 5219 5493 5640 6051 2148
Size: 2673 398 274 147 411 2485
Fragments arranged by size:
2673 2485 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6163
Fragments arranged by size:
6163 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3179 3182 1186
Size: 1993 3 4392
Fragments arranged by size:
4392 1993 3
BseYI C'CCAG_C
Cuts at: 5591 6087 5591
Size: 496 5892
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2624 1163
Size: 573 888 4927
Fragments arranged by size:
4927 888 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3838 4599 5627 1540
Size: 275 186 1837 761 1028 2301
Fragments arranged by size:
2301 1837 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4449 4534 5605 4449
Size: 85 1071 5232
Fragments arranged by size:
5232 1071 85
BsmI GAATG_Cn'
Cuts at: 2096 2184 2964 3899 3992 2096
Size: 88 780 935 93 4492
Fragments arranged by size:
4492 935 780 93 88
BsmBI CGTCTCn'nnnn_
Cuts at: 3347 3347
Size: 6388
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3707 4449 4534 5605 1391
Size: 399 1917 742 85 1071 2174
Fragments arranged by size:
2174 1917 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6051 6051
Size: 6388
BspHI T'CATG_A
Cuts at: 4277 6007 4277
Size: 1730 4658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3743 844
Size: 2899 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4275 1394
Size: 429 2452 3507
Fragments arranged by size:
3507 2452 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2454 3498 4883 5057 65
Size: 1183 1206 1044 1385 174 1396
Fragments arranged by size:
1396 1385 1206 1183 1044 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2148 5032 5219 1700
Size: 110 338 2884 187 2869
Fragments arranged by size:
2884 2869 338 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6388
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6388
BssSI C'ACGA_G
Cuts at: 4448 5460 4448
Size: 1012 5376
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6388
BstBI TT'CG_AA
Cuts at: 5192 5192
Size: 6388
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6388
BstYI r'GATC_y
Cuts at: 1972 2712 3718 4085 4470 4487 5939 6025 6037
Size: 740 1006 367 385 17 1452 86 12
Cuts at: 6037 6383 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1006 740 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2708 2708
Size: 6388
BtgI C'CryG_G
Cuts at: 513 1065 2598 3238 513
Size: 552 1533 640 3663
Fragments arranged by size:
3663 1533 640 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5649
Fragments arranged by size:
5649 683 56
BtsI GCAGTG_nn'
Cuts at: 2038 3904 4698 4718 2038
Size: 1866 794 20 3708
Fragments arranged by size:
3708 1866 794 20
ClaI AT'CG_AT
Cuts at: 1554 3274 1554
Size: 1720 4668
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6353
DraI TTT'AAA
Cuts at: 2443 2529 2574 3692 2443
Size: 86 45 1118 5139
Fragments arranged by size:
5139 1118 86 45
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5395 809
Size: 507 4079 1802
Fragments arranged by size:
4079 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3835 4724 6353
Size: 54 1425 50 275 18 2005 889 1629
Cuts at: 6353 8
Size: 43
Fragments arranged by size:
2005 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3835 1537
Size: 275 2023 4090
Fragments arranged by size:
4090 2023 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4318 1353
Size: 2965 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4975 5349 5495 387
Size: 1064 138 3386 374 146 1280
Fragments arranged by size:
3386 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2776 2837 3012 3114 3397 3414 4451
Size: 393 1144 61 175 102 283 17 1037
Cuts at: 4451 5048 5835 6103 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1144 1037 787 597 393 283 268
175 102 61 17
EcoRI G'AATT_C
Cuts at: 3315 3439 3697 4075 3315
Size: 124 258 378 5628
Fragments arranged by size:
5628 378 258 124
FspI TGC'GCA
Cuts at: 4894 4894
Size: 6388
HaeII r_GCGC'y
Cuts at: 1678 2024 5535 1678
Size: 346 3511 2531
Fragments arranged by size:
3511 2531 346
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5029 5061 5103 5135 1373
Size: 32 135 32 3457 32 42 32 2626
Fragments arranged by size:
3457 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2164 3732 3978 669
Size: 988 281 226 1568 246 3079
Fragments arranged by size:
3079 1568 988 281 246 226
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6388
HpaI GTT'AAC
Cuts at: 3978 3978
Size: 6388
KpnI G_GTAC'C
Cuts at: 3713 3713
Size: 6388
MfeI C'AATT_G
Cuts at: 2865 3987 2865
Size: 1122 5266
MluI A'CGCG_T
Cuts at: 6264 6264
Size: 6388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2334 5502 5686 1305
Size: 1029 3168 184 2007
Fragments arranged by size:
3168 2007 1029 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4566
Fragments arranged by size:
4566 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2316 4346 4705 4864 518
Size: 648 1150 2030 359 159 2042
Fragments arranged by size:
2042 2030 1150 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2170 2824 3677 3804 4481 5629 5874 1606
Size: 564 654 853 127 677 1148 245 2120
Fragments arranged by size:
2120 1148 853 677 654 564 245 127
NcoI C'CATG_G
Cuts at: 513 1065 3238 513
Size: 552 2173 3663
Fragments arranged by size:
3663 2173 552
NdeI CA'TA_TG
Cuts at: 387 2422 387
Size: 2035 4353
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6388
NotI GC'GGCC_GC
Cuts at: 3835 3835
Size: 6388
NsiI A_TGCA'T
Cuts at: 2098 2098
Size: 6388
NspI r_CATG'y
Cuts at: 1132 1229 3746 5291 1132
Size: 97 2517 1545 2229
Fragments arranged by size:
2517 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6388
PciI A'CATG_T
Cuts at: 5287 5287
Size: 6388
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3326 1108
Size: 2218 4170
PfoI T'CCnGG_A
Cuts at: 2548 2548
Size: 6388
PmeI GTTT'AAAC
Cuts at: 3692 3692
Size: 6388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4457 4489 5970 6002 4457
Size: 32 1481 32 4843
Fragments arranged by size:
4843 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6388
PsiI TTA'TAA
Cuts at: 3958 3958
Size: 6388
PstI C_TGCA'G
Cuts at: 830 3740 830
Size: 2910 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6388
PvuII CAG'CTG
Cuts at: 2824 3677 2824
Size: 853 5535
SalI G'TCGA_C
Cuts at: 1936 3730 1936
Size: 1794 4594
SbfI CC_TGCA'GG
Cuts at: 3740 3740
Size: 6388
ScaI AGT'ACT
Cuts at: 4636 6348 4636
Size: 1712 4676
SfcI C'TryA_G
Cuts at: 826 1045 3736 4871 5552 5743 826
Size: 219 2691 1135 681 191 1471
Fragments arranged by size:
2691 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6388
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6388
SmaI CCC'GGG
Cuts at: 1872 3715 1872
Size: 1843 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4492 5393 5655 820
Size: 197 926 2549 901 262 1553
Fragments arranged by size:
2549 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4179 493
Size: 3686 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6388
SphI G_CATG'C
Cuts at: 1132 3746 1132
Size: 2614 3774
SspI AAT'ATT
Cuts at: 5 52 4312 5
Size: 47 4260 2081
Fragments arranged by size:
4260 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6388
StyI C'CwwG_G
Cuts at: 513 1065 2994 3238 3827 513
Size: 552 1929 244 589 3074
Fragments arranged by size:
3074 1929 589 552 244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4415 4432 4585 4770 5215 325
Size: 1255 2835 17 153 185 445 1498
Fragments arranged by size:
2835 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2839 4634 6346
Size: 274 80 33 51 1443 862 1795 1712
Cuts at: 6346 96
Size: 138
Fragments arranged by size:
1795 1712 1443 862 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6348 6380 6348
Size: 32 6356
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6388
XbaI T'CTAG_A
Cuts at: 3265 3724 3265
Size: 459 5929
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6388
XmaI C'CCGG_G
Cuts at: 1870 3713 1870
Size: 1843 4545
XmnI GAAnn'nnTTC
Cuts at: 2402 2964 3427 4517 2402
Size: 562 463 1090 4273
Fragments arranged by size:
4273 1090 562 463
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4196 276
Size: 53 83 186 936 2662 2468
Fragments arranged by size:
2662 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI