################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII AccI AccII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AspI AviII AvrII BalI BamHI BbeI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BlnI BlpI Bpu1102I BsaHI BsaI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KspI MaeI MfeI MluI MluNI MscI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 58 GGTACC G^GTACC AccIII 284 TCCGGA T^CCGGA AlwI 664 GATCC GGATC(4/5) AlwNI 469 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 58 GGTACC G^GTACC BanII 639 GGGCTC G[GA]GC[CT]^C BbrPI 605 CACGTG CAC^GTG BbsI 288 GAAGAC GAAGAC(2/6) BglI 632 GCCCTTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 740 AGATCT A^GATCT BmyI 639 GGGCTC G[AGT]GC[ACT]^C BpuAI 288 GAAGAC GAAGAC(2/6) BsaAI 605 CACGTG [CT]AC^GT[GA] BsaBI 774 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 284 TCCGGA [AT]^CCGG[AT] BseAI 284 TCCGGA T^CCGGA Bsp1286I 639 GGGCTC G[AGT]GC[ACT]^C BspEI 284 TCCGGA T^CCGGA BsrBI 355 GAGCGG CCGCTC(-3/-3) BsrDI 152 GCAATG GCAATG(2/0) BstXI 600 CCAAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 740 AGATCT [GA]^GATC[CT] CfoI 472 GCGC GCG^C DraII 173 AGGCCCT [GA]G^G[ACGT]CC[CT] EaeI 723 TGGCCG [CT]^GGCC[GA] EcoO109I 173 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoT14I 931 CCAAGG C^C[AT][AT]GG FauI 357 GCGGG CCCGC(4/6) HaeII 471 GGCGCT [GA]GCGC^[CT] HapII 285 CCGG C^CGG HgaI 534 GCGTC GACGC(5/10) HhaI 472 GCGC GCG^C HinP1I 472 GCGC G^CGC HpaII 285 CCGG C^CGG HphI 836 TCACC GGTGA(8/7) KpnI 58 GGTACC GGTAC^C MamI 774 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 740 AGATCT [GA]^GATC[CT] MroI 284 TCCGGA T^CCGGA MslI 819 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 285 CCGG C^CGG NlaIII 656 CATG CATG^ PflMI 236 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 605 CACGTG CAC^GTG PmlI 605 CACGTG CAC^GTG StyI 931 CCAAGG C^C[AT][AT]GG TaqI 804 TCGA T^CGA TfiI 806 GAATC G^A[AT]TC Tsp45I 229 GTGAC ^GT[GC]AC TthHB8I 804 TCGA T^CGA Van91I 236 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 740 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AvaI 212 CTCGGG C^[CT]CG[GA]G 262 CCCGAG BanI 58 GGTACC G^G[CT][GA]CC 207 GGTGCC BseRI 642 CTCCTC GAGGAG(10/8) 217 GAGGAG BsgI 22 CTGCAC GTGCAG(16/14) 466 GTGCAG BsmAI 362 GAGAC GTCTC(1/5) 424 GAGAC BsmI 381 GAATGC GAATGC(1/-1) 556 GAATGC BsoBI 212 CTCGGG C^[CT]CG[GA]G 262 CCCGAG BspMI 549 GCAGGT ACCTGC(4/8) 756 GCAGGT Cac8I 276 GCCTGC GC[ACGT]^[ACGT]GC 468 GCAGGC EcoNI 177 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 681 CCTGCTGGAGG MaeII 188 ACGT A^CGT 606 ACGT MaeIII 229 GTGAC ^GT[ACGT]AC 619 GTAAC MseI 98 TTAA T^TAA 962 TTAA Tru9I 98 TTAA T^TAA 962 TTAA Tsp509I 588 AATT ^AATT 883 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI AfaI AluI AvaII BbvI BpmI BsaJI BsiYI BslI BsmFI BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MboI MboII MnlI MspA1I MvaI MwoI NdeII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI TseI TspRI