(Circular) (Six-base) MAPSORT of: pFN21AE4319.seq Check: 2910 from: 1 to: 5680 pFN21AE4319 5680 bp With 182 enzymes: SgfI * March 28, 2012 14:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2545 2545 Size: 5680 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3490 278 Size: 53 83 186 936 1954 2468 Fragments arranged by size: 2468 1954 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3023 1937 Size: 1086 4594 Acc65I G'GTAC_C Cuts at: 2063 3001 2063 Size: 938 4742 AclI AA'CG_TT Cuts at: 1563 3807 4180 1563 Size: 2244 373 3063 Fragments arranged by size: 3063 2244 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2204 2801 3743 5127 5395 1632 Size: 572 597 942 1384 268 1917 Fragments arranged by size: 1917 1384 942 597 572 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5483 AflIII A'CryG_T Cuts at: 1203 4579 5556 1203 Size: 3376 977 1327 Fragments arranged by size: 3376 1327 977 AgeI A'CCGG_T Cuts at: 4511 4511 Size: 5680 AhdI GACnn_n'nnGTC Cuts at: 4409 4409 Size: 5680 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5680 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2228 2889 1184 Size: 1044 661 3975 Fragments arranged by size: 3975 1044 661 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2688 2720 2688 Size: 32 5648 AlwNI CAG_nnn'CTG Cuts at: 2479 3423 4995 2479 Size: 944 1572 3164 Fragments arranged by size: 3164 1572 944 ApaLI G'TGCA_C Cuts at: 3737 4893 3737 Size: 1156 4524 ApoI r'AATT_y Cuts at: 1493 2989 3215 3367 5476 1493 Size: 1496 226 152 2109 1697 Fragments arranged by size: 2109 1697 1496 226 152 AseI AT'TA_AT Cuts at: 160 1032 4234 160 Size: 872 3202 1606 Fragments arranged by size: 3202 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5680 AvaI C'yCGr_G Cuts at: 1870 1943 2217 2267 3005 1870 Size: 73 274 50 738 4545 Fragments arranged by size: 4545 738 274 73 50 BamHI G'GATC_C Cuts at: 3010 3377 3010 Size: 367 5313 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2063 2212 3001 4456 618 Size: 325 217 627 276 149 789 1455 1842 Fragments arranged by size: 1842 1455 789 627 325 276 217 149 BanII G_rGCy'C Cuts at: 1391 2648 2999 1391 Size: 1257 351 4072 Fragments arranged by size: 4072 1257 351 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2300 928 Size: 971 401 4308 Fragments arranged by size: 4308 971 401 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3871 3905 1903 Size: 34 1934 34 3678 Fragments arranged by size: 3678 1934 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3572 4782 3572 Size: 1210 4470 BclI T'GATC_A Cuts at: 1548 1548 Size: 5680 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2643 4291 Size: 107 122 71 631 605 337 634 1648 Cuts at: 4291 136 Size: 1525 Fragments arranged by size: 1648 1525 634 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2745 5675 2745 Size: 2930 2750 BlpI GC'TnA_GC Cuts at: 3097 3097 Size: 5680 Bme1580I G_kGCm'C Cuts at: 1790 3741 4897 1790 Size: 1951 1156 2573 Fragments arranged by size: 2573 1951 1156 BmgBI CAC'GTC Cuts at: 1318 2194 1318 Size: 876 4804 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3411 4369 442 Size: 416 53 83 399 24 1994 958 1753 Fragments arranged by size: 1994 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5680 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2294 2336 2465 2639 2711 2721 4340 1239 Size: 1055 42 129 174 72 10 1619 2579 Fragments arranged by size: 2579 1619 1055 174 129 72 42 10 Bpu10I CC'TnA_GC Cuts at: 2114 2460 2753 2114 Size: 346 293 5041 Fragments arranged by size: 5041 346 293 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3805 4670 4968 3805 Size: 865 298 4517 Fragments arranged by size: 4517 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4343 882 Size: 617 2844 2219 Fragments arranged by size: 2844 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2612 3471 493 Size: 711 1408 859 2702 Fragments arranged by size: 2702 1408 859 711 BsaBI GATnn'nnATC Cuts at: 2783 5674 2783 Size: 2891 2789 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3487 3869 4504 275 Size: 53 83 186 936 1954 382 635 1451 Fragments arranged by size: 1954 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2289 4113 4511 4785 4932 5343 2289 Size: 1824 398 274 147 411 2626 Fragments arranged by size: 2626 1824 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2213 2243 1178 Size: 30 165 30 810 30 4615 Fragments arranged by size: 4615 810 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2237 2638 1186 Size: 1051 401 4228 Fragments arranged by size: 4228 1051 401 BseYI C'CCAG_C Cuts at: 2479 4883 5379 2479 Size: 2404 496 2780 Fragments arranged by size: 2780 2404 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2012 2492 1163 Size: 573 276 480 4351 Fragments arranged by size: 4351 573 480 276 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3130 3891 4919 1540 Size: 275 186 1129 761 1028 2301 Fragments arranged by size: 2301 1129 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3741 3826 4897 3741 Size: 85 1071 4524 Fragments arranged by size: 4524 1071 85 BsiWI C'GTAC_G Cuts at: 2976 2976 Size: 5680 BsmI GAATG_Cn' Cuts at: 2392 2567 3191 3284 2392 Size: 175 624 93 4788 Fragments arranged by size: 4788 624 175 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2648 2999 3741 3826 4897 1391 Size: 399 858 351 742 85 1071 2174 Fragments arranged by size: 2174 1071 858 742 399 351 85 BspEI T'CCGG_A Cuts at: 2289 5343 2289 Size: 3054 2626 BspHI T'CATG_A Cuts at: 3569 5299 3569 Size: 1730 3950 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2545 2752 3035 844 Size: 1701 207 283 3489 Fragments arranged by size: 3489 1701 283 207 BsrBI CCG'CTC Cuts at: 1394 1823 2362 3567 1394 Size: 429 539 1205 3507 Fragments arranged by size: 3507 1205 539 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2164 4175 4349 65 Size: 1183 916 2011 174 1396 Fragments arranged by size: 2011 1396 1183 916 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4324 4511 1700 Size: 110 2514 187 2869 Fragments arranged by size: 2869 2514 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5680 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5680 BssSI C'ACGA_G Cuts at: 3740 4752 3740 Size: 1012 4668 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5680 BstBI TT'CG_AA Cuts at: 4484 4484 Size: 5680 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5680 BstXI CCAn_nnnn'nTGG Cuts at: 2612 2612 Size: 5680 BstYI r'GATC_y Cuts at: 1972 2745 3010 3377 3762 3779 5231 5317 5329 Size: 773 265 367 385 17 1452 86 12 Cuts at: 5329 5675 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 773 385 367 346 265 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4189 Fragments arranged by size: 4189 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4941 Fragments arranged by size: 4941 683 56 BtsI GCAGTG_nn' Cuts at: 2599 3196 3990 4010 2599 Size: 597 794 20 4269 Fragments arranged by size: 4269 794 597 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5680 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2120 2155 529 Size: 35 1556 35 4054 Fragments arranged by size: 4054 1556 35 35 DraI TTT'AAA Cuts at: 2984 2984 Size: 5680 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4687 809 Size: 507 3371 1802 Fragments arranged by size: 3371 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2728 3127 4016 Size: 54 1425 50 275 18 898 399 889 Cuts at: 4016 5645 8 Size: 1629 43 Fragments arranged by size: 1629 1425 898 889 399 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3127 1537 Size: 275 1315 4090 Fragments arranged by size: 4090 1315 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2066 2850 2921 3610 1353 Size: 713 784 71 689 3423 Fragments arranged by size: 3423 784 713 689 71 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4267 4641 4787 387 Size: 1064 138 2678 374 146 1280 Fragments arranged by size: 2678 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2204 2294 2336 2465 2639 2711 2721 Size: 393 572 90 42 129 174 72 10 Cuts at: 2721 2801 3743 4340 5127 5395 1239 Size: 80 942 597 787 268 1524 Fragments arranged by size: 1524 942 787 597 572 393 268 174 129 90 80 72 42 10 EcoNI CCTnn'n_nnAGG Cuts at: 2186 2690 2186 Size: 504 5176 EcoO109I rG'GnC_Cy Cuts at: 2179 2179 Size: 5680 EcoRI G'AATT_C Cuts at: 2989 3367 2989 Size: 378 5302 FspI TGC'GCA Cuts at: 4186 4186 Size: 5680 HaeII r_GCGC'y Cuts at: 1678 2480 4827 1678 Size: 802 2347 2531 Fragments arranged by size: 2531 2347 802 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4321 4353 4395 4427 1373 Size: 32 135 32 2749 32 42 32 2626 Fragments arranged by size: 2749 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3024 3270 669 Size: 988 281 1086 246 3079 Fragments arranged by size: 3079 1086 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5680 HpaI GTT'AAC Cuts at: 3270 3270 Size: 5680 KpnI G_GTAC'C Cuts at: 2067 3005 2067 Size: 938 4742 MfeI C'AATT_G Cuts at: 3279 3279 Size: 5680 MluI A'CGCG_T Cuts at: 5556 5556 Size: 5680 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2514 4794 4978 1305 Size: 1209 2280 184 2007 Fragments arranged by size: 2280 2007 1209 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3858 Fragments arranged by size: 3858 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2828 3638 3997 4156 518 Size: 648 1662 810 359 159 2042 Fragments arranged by size: 2042 1662 810 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2099 2314 2546 2770 3096 3773 4921 5166 Size: 493 215 232 224 326 677 1148 245 Cuts at: 5166 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 493 326 245 232 224 215 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4189 Fragments arranged by size: 4189 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5680 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5680 NotI GC'GGCC_GC Cuts at: 3127 3127 Size: 5680 NspI r_CATG'y Cuts at: 1132 1229 3038 4583 1132 Size: 97 1809 1545 2229 Fragments arranged by size: 2229 1809 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5680 PciI A'CATG_T Cuts at: 4579 4579 Size: 5680 PflMI CCAn_nnn'nTGG Cuts at: 1108 2247 1108 Size: 1139 4541 PmeI GTTT'AAAC Cuts at: 2984 2984 Size: 5680 PmlI CAC'GTG Cuts at: 2612 2612 Size: 5680 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3749 3781 5262 5294 3749 Size: 32 1481 32 4135 Fragments arranged by size: 4135 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5680 PsiI TTA'TAA Cuts at: 3250 3250 Size: 5680 PstI C_TGCA'G Cuts at: 830 3032 830 Size: 2202 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5680 PvuII CAG'CTG Cuts at: 2099 2314 2546 2099 Size: 215 232 5233 Fragments arranged by size: 5233 232 215 SalI G'TCGA_C Cuts at: 1936 3022 1936 Size: 1086 4594 SbfI CC_TGCA'GG Cuts at: 3032 3032 Size: 5680 ScaI AGT'ACT Cuts at: 3928 5640 3928 Size: 1712 3968 SfcI C'TryA_G Cuts at: 826 1045 3028 4163 4844 5035 826 Size: 219 1983 1135 681 191 1471 Fragments arranged by size: 1983 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5680 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5680 SmaI CCC'GGG Cuts at: 1872 3007 1872 Size: 1135 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3784 4685 4947 820 Size: 197 926 1841 901 262 1553 Fragments arranged by size: 1841 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3471 493 Size: 2978 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5680 SphI G_CATG'C Cuts at: 1132 3038 1132 Size: 1906 3774 SspI AAT'ATT Cuts at: 5 52 3604 5 Size: 47 3552 2081 Fragments arranged by size: 3552 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5680 StyI C'CwwG_G Cuts at: 513 1065 2004 2936 3119 513 Size: 552 939 932 183 3074 Fragments arranged by size: 3074 939 932 552 183 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2432 2583 3707 3724 3877 4062 4507 Size: 1255 852 151 1124 17 153 185 445 Cuts at: 4507 325 Size: 1498 Fragments arranged by size: 1498 1255 1124 852 445 185 153 151 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3926 5638 96 Size: 274 80 33 51 1443 1949 1712 138 Fragments arranged by size: 1949 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5640 5672 5640 Size: 32 5648 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5680 XbaI T'CTAG_A Cuts at: 3016 3016 Size: 5680 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5680 XmaI C'CCGG_G Cuts at: 1870 3005 1870 Size: 1135 4545 XmnI GAAnn'nnTTC Cuts at: 3809 3809 Size: 5680 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3488 276 Size: 53 83 186 936 1954 2468 Fragments arranged by size: 2468 1954 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI