(Linear) (Six-base) MAPSORT of: KIEE4319.seq Check: 6475 from: 1 to: 975 KIEE4319.orf With 182 enzymes: SgfI * March 22, 2012 17:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 540 975 Size: 540 435 Acc65I G'GTAC_C Cuts at: 0 58 975 Size: 58 917 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 199 796 975 Size: 199 597 179 Fragments arranged by size: 597 199 179 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 223 884 975 Size: 223 661 91 Fragments arranged by size: 661 223 91 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 683 715 975 Size: 683 32 260 Fragments arranged by size: 683 260 32 AlwNI CAG_nnn'CTG Cuts at: 0 474 975 Size: 474 501 AvaI C'yCGr_G Cuts at: 0 212 262 975 Size: 212 50 713 Fragments arranged by size: 713 212 50 BanI G'GyrC_C Cuts at: 0 58 207 975 Size: 58 149 768 Fragments arranged by size: 768 149 58 BanII G_rGCy'C Cuts at: 0 643 975 Size: 643 332 BbsI GAAGACnn'nnnn_ Cuts at: 0 295 975 Size: 295 680 BglI GCCn_nnn'nGGC Cuts at: 0 638 975 Size: 638 337 BglII A'GATC_T Cuts at: 0 740 975 Size: 740 235 BmgBI CAC'GTC Cuts at: 0 189 975 Size: 189 786 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 289 331 460 634 706 716 975 Size: 289 42 129 174 72 10 259 Fragments arranged by size: 289 259 174 129 72 42 10 Bpu10I CC'TnA_GC Cuts at: 0 109 455 748 975 Size: 109 346 293 227 Fragments arranged by size: 346 293 227 109 BsaAI yAC'GTr Cuts at: 0 607 975 Size: 607 368 BsaBI GATnn'nnATC Cuts at: 0 778 975 Size: 778 197 BsaWI w'CCGG_w Cuts at: 0 284 975 Size: 284 691 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 208 238 975 Size: 208 30 737 Fragments arranged by size: 737 208 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 232 633 975 Size: 232 401 342 Fragments arranged by size: 401 342 232 BseYI C'CCAG_C Cuts at: 0 474 975 Size: 474 501 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 7 487 975 Size: 7 480 488 Fragments arranged by size: 488 480 7 BsmI GAATG_Cn' Cuts at: 0 387 562 975 Size: 387 175 413 Fragments arranged by size: 413 387 175 Bsp1286I G_dGCh'C Cuts at: 0 643 975 Size: 643 332 BspEI T'CCGG_A Cuts at: 0 284 975 Size: 284 691 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 540 747 975 Size: 540 207 228 Fragments arranged by size: 540 228 207 BsrBI CCG'CTC Cuts at: 0 357 975 Size: 357 618 BsrDI GCAATG_nn' Cuts at: 0 159 975 Size: 159 816 BstXI CCAn_nnnn'nTGG Cuts at: 0 607 975 Size: 607 368 BstYI r'GATC_y Cuts at: 0 740 975 Size: 740 235 BtsI GCAGTG_nn' Cuts at: 0 594 975 Size: 594 381 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 115 150 975 Size: 115 35 825 Fragments arranged by size: 825 115 35 EaeI y'GGCC_r Cuts at: 0 723 975 Size: 723 252 EarI CTCTTCn'nnn_ Cuts at: 0 61 845 916 975 Size: 61 784 71 59 Fragments arranged by size: 784 71 61 59 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 199 289 331 460 634 706 716 796 Size: 199 90 42 129 174 72 10 80 Cuts at: 796 975 Size: 179 Fragments arranged by size: 199 179 174 129 90 80 72 42 10 EcoNI CCTnn'n_nnAGG Cuts at: 0 181 685 975 Size: 181 504 290 Fragments arranged by size: 504 290 181 EcoO109I rG'GnC_Cy Cuts at: 0 174 975 Size: 174 801 HaeII r_GCGC'y Cuts at: 0 475 975 Size: 475 500 KpnI G_GTAC'C Cuts at: 0 62 975 Size: 62 913 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 509 975 Size: 509 466 MslI CAynn'nnrTG Cuts at: 0 823 975 Size: 823 152 MspA1I CmG'CkG Cuts at: 0 94 309 541 765 975 Size: 94 215 232 224 210 Fragments arranged by size: 232 224 215 210 94 PflMI CCAn_nnn'nTGG Cuts at: 0 242 975 Size: 242 733 PmlI CAC'GTG Cuts at: 0 607 975 Size: 607 368 PvuII CAG'CTG Cuts at: 0 94 309 541 975 Size: 94 215 232 434 Fragments arranged by size: 434 232 215 94 StyI C'CwwG_G Cuts at: 0 931 975 Size: 931 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 427 578 975 Size: 427 151 397 Fragments arranged by size: 427 397 151 Enzymes that do cut: AarI Acc65I AcuI AlfI AloI AlwNI AvaI BanI BanII BbsI BglI BglII BmgBI BpmI Bpu10I BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BstXI BstYI BtsI CspCI EaeI EarI Eco57MI EcoNI EcoO109I HaeII KpnI MmeI MslI MspA1I PflMI PmlI PvuII StyI TaqII Enzymes that do not cut: AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BlpI Bme1580I BmrI BmtI BplI BpuEI BsaI BsaHI BsiEI BsiHKAI BsiWI BsmBI BspHI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI DraI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KasI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI