(Circular) (Six-base) MAPSORT of: pFN21AB3985.seq Check: 811 from: 1 to: 5713 pFN21AB3985 5713 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3523 278 Size: 53 83 186 936 1987 2468 Fragments arranged by size: 2468 1987 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2559 3056 1937 Size: 622 497 4594 Fragments arranged by size: 4594 622 497 Acc65I G'GTAC_C Cuts at: 3034 3034 Size: 5713 AclI AA'CG_TT Cuts at: 1563 3840 4213 1563 Size: 2277 373 3063 Fragments arranged by size: 3063 2277 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2621 2784 3776 5160 5428 1632 Size: 989 163 992 1384 268 1917 Fragments arranged by size: 1917 1384 992 989 268 163 AfeI AGC'GCT Cuts at: 2046 2046 Size: 5713 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5516 AflIII A'CryG_T Cuts at: 1203 4612 5589 1203 Size: 3409 977 1327 Fragments arranged by size: 3409 1327 977 AgeI A'CCGG_T Cuts at: 4544 4544 Size: 5713 AhdI GACnn_n'nnGTC Cuts at: 4442 4442 Size: 5713 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5713 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2549 1184 Size: 1365 4348 AlwNI CAG_nnn'CTG Cuts at: 2289 2590 3456 5028 2289 Size: 301 866 1572 2974 Fragments arranged by size: 2974 1572 866 301 ApaI G_GGCC'C Cuts at: 2522 2522 Size: 5713 ApaLI G'TGCA_C Cuts at: 2059 3770 4926 2059 Size: 1711 1156 2846 Fragments arranged by size: 2846 1711 1156 ApoI r'AATT_y Cuts at: 1493 3022 3248 3400 5509 1493 Size: 1529 226 152 2109 1697 Fragments arranged by size: 2109 1697 1529 226 152 AseI AT'TA_AT Cuts at: 160 1032 4267 160 Size: 872 3235 1606 Fragments arranged by size: 3235 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5713 AvaI C'yCGr_G Cuts at: 1870 1943 2207 3038 1870 Size: 73 264 831 4545 Fragments arranged by size: 4545 831 264 73 BamHI G'GATC_C Cuts at: 2457 2785 3043 3410 2457 Size: 328 258 367 4760 Fragments arranged by size: 4760 367 328 258 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2498 2764 3034 4489 618 Size: 325 217 627 711 266 270 1455 1842 Fragments arranged by size: 1842 1455 711 627 325 270 266 217 BanII G_rGCy'C Cuts at: 1391 2522 2649 2736 3032 1391 Size: 1131 127 87 296 4072 Fragments arranged by size: 4072 1131 296 127 87 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2015 928 Size: 971 116 4626 Fragments arranged by size: 4626 971 116 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3904 3938 1903 Size: 34 1967 34 3678 Fragments arranged by size: 3678 1967 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2234 3605 4815 2234 Size: 1371 1210 3132 Fragments arranged by size: 3132 1371 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5713 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2094 4324 136 Size: 107 122 71 631 605 422 2230 1525 Fragments arranged by size: 2230 1525 631 605 422 122 107 71 BglII A'GATC_T Cuts at: 5708 5708 Size: 5713 BlpI GC'TnA_GC Cuts at: 2696 3130 2696 Size: 434 5279 Bme1580I G_kGCm'C Cuts at: 1790 2063 2296 2501 2522 2769 2929 3774 4930 Size: 273 233 205 21 247 160 845 1156 Cuts at: 4930 1790 Size: 2573 Fragments arranged by size: 2573 1156 845 273 247 233 205 160 21 BmgBI CAC'GTC Cuts at: 1318 2407 1318 Size: 1089 4624 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2932 3444 4402 Size: 416 53 83 399 24 1515 512 958 Cuts at: 4402 442 Size: 1753 Fragments arranged by size: 1753 1515 958 512 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5713 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2750 4373 1239 Size: 1511 1623 2579 Fragments arranged by size: 2579 1623 1511 Bpu10I CC'TnA_GC Cuts at: 2308 2579 2308 Size: 271 5442 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3838 4703 5001 3838 Size: 865 298 4550 Fragments arranged by size: 4550 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4376 882 Size: 617 2877 2219 Fragments arranged by size: 2877 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3504 493 Size: 711 2300 2702 Fragments arranged by size: 2702 2300 711 BsaBI GATnn'nnATC Cuts at: 2790 5707 2790 Size: 2917 2796 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2153 2379 3520 3902 Size: 53 83 186 936 620 226 1141 382 Cuts at: 3902 4537 275 Size: 635 1451 Fragments arranged by size: 1451 1141 936 635 620 382 226 186 83 53 BsaWI w'CCGG_w Cuts at: 4146 4544 4818 4965 5376 4146 Size: 398 274 147 411 4483 Fragments arranged by size: 4483 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5488 Fragments arranged by size: 5488 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5713 BseYI C'CCAG_C Cuts at: 2196 2522 4916 5412 2196 Size: 326 2394 496 2497 Fragments arranged by size: 2497 2394 496 326 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2306 1163 Size: 573 570 4570 Fragments arranged by size: 4570 573 570 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3163 3924 4952 1540 Size: 275 186 1162 761 1028 2301 Fragments arranged by size: 2301 1162 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2063 2649 2736 3774 3859 4930 2063 Size: 586 87 1038 85 1071 2846 Fragments arranged by size: 2846 1071 1038 586 87 85 BsmI GAATG_Cn' Cuts at: 2440 3224 3317 2440 Size: 784 93 4836 Fragments arranged by size: 4836 784 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2063 2296 2501 2522 2649 2736 2769 Size: 399 273 233 205 21 127 87 33 Cuts at: 2769 2929 3032 3774 3859 4930 1391 Size: 160 103 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 273 233 205 160 127 103 87 85 33 21 BspEI T'CCGG_A Cuts at: 5376 5376 Size: 5713 BspHI T'CATG_A Cuts at: 2610 3602 5332 2610 Size: 992 1730 2991 Fragments arranged by size: 2991 1730 992 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2487 2577 3068 844 Size: 1643 90 491 3489 Fragments arranged by size: 3489 1643 491 90 BsrBI CCG'CTC Cuts at: 1394 1823 2107 3600 1394 Size: 429 284 1493 3507 Fragments arranged by size: 3507 1493 429 284 BsrDI GCAATG_nn' Cuts at: 65 1248 4208 4382 65 Size: 1183 2960 174 1396 Fragments arranged by size: 2960 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2162 2176 4357 4544 1700 Size: 110 352 14 2181 187 2869 Fragments arranged by size: 2869 2181 352 187 110 14 BsrGI T'GTAC_A Cuts at: 96 2079 2843 96 Size: 1983 764 2966 Fragments arranged by size: 2966 1983 764 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5713 BssSI C'ACGA_G Cuts at: 3773 4785 3773 Size: 1012 4701 BstAPI GCAn_nnn'nTGC Cuts at: 794 2320 794 Size: 1526 4187 BstBI TT'CG_AA Cuts at: 4517 4517 Size: 5713 BstEII G'GTnAC_C Cuts at: 1184 2299 1184 Size: 1115 4598 BstYI r'GATC_y Cuts at: 1972 2457 2785 3043 3410 3795 3812 5264 5350 Size: 485 328 258 367 385 17 1452 86 Cuts at: 5350 5362 5708 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 485 385 367 346 328 258 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2426 2426 Size: 5713 BtgI C'CryG_G Cuts at: 513 1065 2023 2217 2225 513 Size: 552 958 194 8 4001 Fragments arranged by size: 4001 958 552 194 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2419 487 Size: 683 56 1193 3781 Fragments arranged by size: 3781 1193 683 56 BtsI GCAGTG_nn' Cuts at: 2040 2278 3229 4023 4043 2040 Size: 238 951 794 20 3710 Fragments arranged by size: 3710 951 794 238 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5713 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5678 DraI TTT'AAA Cuts at: 3017 3017 Size: 5713 DraIII CAC_nnn'GTG Cuts at: 2058 2058 Size: 5713 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4720 809 Size: 507 3404 1802 Fragments arranged by size: 3404 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2095 2157 2228 Size: 54 1425 50 275 18 265 62 71 Cuts at: 2228 3160 4049 5678 8 Size: 932 889 1629 43 Fragments arranged by size: 1629 1425 932 889 275 265 71 62 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3160 1537 Size: 275 1348 4090 Fragments arranged by size: 4090 1348 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2654 3643 1353 Size: 1301 989 3423 Fragments arranged by size: 3423 1301 989 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2198 2270 4300 4674 4820 387 Size: 1064 138 609 72 2030 374 146 1280 Fragments arranged by size: 2030 1280 1064 609 374 146 138 72 EcoICRI GAG'CTC Cuts at: 2647 2734 2647 Size: 87 5626 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2621 2750 2784 3776 4373 5160 5428 Size: 393 989 129 34 992 597 787 268 Cuts at: 5428 1239 Size: 1524 Fragments arranged by size: 1524 992 989 787 597 393 268 129 34 EcoNI CCTnn'n_nnAGG Cuts at: 2582 2582 Size: 5713 EcoO109I rG'GnC_Cy Cuts at: 2212 2518 2519 2212 Size: 306 1 5406 Fragments arranged by size: 5406 306 1 EcoRI G'AATT_C Cuts at: 3022 3400 3022 Size: 378 5335 EcoRV GAT'ATC Cuts at: 2682 2682 Size: 5713 FspI TGC'GCA Cuts at: 4219 4219 Size: 5713 HaeII r_GCGC'y Cuts at: 1678 2048 2206 4860 1678 Size: 370 158 2654 2531 Fragments arranged by size: 2654 2531 370 158 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4354 4386 4428 4460 1373 Size: 32 135 32 2782 32 42 32 2626 Fragments arranged by size: 2782 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3057 3303 669 Size: 988 281 1119 246 3079 Fragments arranged by size: 3079 1119 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5713 HpaI GTT'AAC Cuts at: 3303 3303 Size: 5713 KpnI G_GTAC'C Cuts at: 3038 3038 Size: 5713 MfeI C'AATT_G Cuts at: 2721 3312 2721 Size: 591 5122 MluI A'CGCG_T Cuts at: 5589 5589 Size: 5713 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2709 4827 5011 1305 Size: 1404 2118 184 2007 Fragments arranged by size: 2118 2007 1404 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3891 Fragments arranged by size: 3891 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2054 2369 2817 3671 4030 4189 518 Size: 648 888 315 448 854 359 159 2042 Fragments arranged by size: 2042 888 854 648 448 359 315 159 MspA1I CmG'CkG Cuts at: 1606 2219 2227 3129 3806 4954 5199 1606 Size: 613 8 902 677 1148 245 2120 Fragments arranged by size: 2120 1148 902 677 613 245 8 NaeI GCC'GGC Cuts at: 2178 2178 Size: 5713 NcoI C'CATG_G Cuts at: 513 1065 2023 513 Size: 552 958 4203 Fragments arranged by size: 4203 958 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5713 NgoMIV G'CCGG_C Cuts at: 2176 2176 Size: 5713 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5713 NotI GC'GGCC_GC Cuts at: 3160 3160 Size: 5713 NspI r_CATG'y Cuts at: 1132 1229 2536 3071 4616 1132 Size: 97 1307 535 1545 2229 Fragments arranged by size: 2229 1545 1307 535 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5713 PciI A'CATG_T Cuts at: 4612 4612 Size: 5713 PflMI CCAn_nnn'nTGG Cuts at: 1108 2029 2691 1108 Size: 921 662 4130 Fragments arranged by size: 4130 921 662 PmeI GTTT'AAAC Cuts at: 3017 3017 Size: 5713 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3782 3814 5295 5327 3782 Size: 32 1481 32 4168 Fragments arranged by size: 4168 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2212 2212 Size: 5713 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5713 PsiI TTA'TAA Cuts at: 3283 3283 Size: 5713 PspOMI G'GGCC_C Cuts at: 2518 2518 Size: 5713 PstI C_TGCA'G Cuts at: 830 2285 2637 2783 3065 830 Size: 1455 352 146 282 3478 Fragments arranged by size: 3478 1455 352 282 146 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5713 SacI G_AGCT'C Cuts at: 2649 2736 2649 Size: 87 5626 SacII CC_GC'GG Cuts at: 2220 2228 2220 Size: 8 5705 SalI G'TCGA_C Cuts at: 1936 3055 1936 Size: 1119 4594 SanDI GG'GwC_CC Cuts at: 2212 2212 Size: 5713 SapI GCTCTTCn'nnn_ Cuts at: 2654 2654 Size: 5713 SbfI CC_TGCA'GG Cuts at: 3065 3065 Size: 5713 ScaI AGT'ACT Cuts at: 3961 5673 3961 Size: 1712 4001 SfcI C'TryA_G Cuts at: 826 1045 2281 2633 2779 3061 4196 4877 5068 Size: 219 1236 352 146 282 1135 681 191 Cuts at: 5068 826 Size: 1471 Fragments arranged by size: 1471 1236 1135 681 352 282 219 191 146 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5713 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5713 SmaI CCC'GGG Cuts at: 1872 2209 3040 1872 Size: 337 831 4545 Fragments arranged by size: 4545 831 337 SmlI C'TyrA_G Cuts at: 820 1017 1943 3817 4718 4980 820 Size: 197 926 1874 901 262 1553 Fragments arranged by size: 1874 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3504 493 Size: 3011 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5713 SphI G_CATG'C Cuts at: 1132 3071 1132 Size: 1939 3774 SspI AAT'ATT Cuts at: 5 52 2941 3637 5 Size: 47 2889 696 2081 Fragments arranged by size: 2889 2081 696 47 StuI AGG'CCT Cuts at: 1517 2606 1517 Size: 1089 4624 StyI C'CwwG_G Cuts at: 513 1065 2023 3152 513 Size: 552 958 1129 3074 Fragments arranged by size: 3074 1129 958 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2210 3740 3757 3910 4095 4540 325 Size: 1255 630 1530 17 153 185 445 1498 Fragments arranged by size: 1530 1498 1255 630 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2079 2843 3959 Size: 274 80 33 51 1443 102 764 1116 Cuts at: 3959 5671 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1116 764 274 138 102 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5673 5705 5673 Size: 32 5681 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5713 XbaI T'CTAG_A Cuts at: 3049 3049 Size: 5713 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5713 XmaI C'CCGG_G Cuts at: 1870 2207 3038 1870 Size: 337 831 4545 Fragments arranged by size: 4545 831 337 XmnI GAAnn'nnTTC Cuts at: 2606 3842 2606 Size: 1236 4477 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3521 276 Size: 53 83 186 936 1987 2468 Fragments arranged by size: 2468 1987 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SexAI SfiI SfoI SrfI SwaI XcmI