Restriction map for
pFN21AB3981
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AvaII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiWI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
Cfr10I Cfr13I ClaI CpoI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
HaeII HaeIII HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MaeII MaeIII
MamI MfeI MluI MluNI MroI
MscI MslI MspA1I MspI MunI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI TfiI Tsp45I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 445 CGCG CG^CG
AfaI 166 GTAC GT^AC
Alw44I 35 GTGCAC G^TGCAC
AlwI 462 GATCC GGATC(4/5)
AlwNI 398 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 35 GTGCAC G^TGCAC
AspI 448 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 20 TGCGCA TGC^GCA
BbsI 118 GAAGAC GAAGAC(2/6)
BfaI 319 CTAG C^TAG
BlpI 473 GCTCAGC GC^T[ACGT]AGC
BpmI 331 CTGGAG CTGGAG(16/14)
Bpu1102I 473 GCTCAGC GC^T[ACGT]AGC
BpuAI 118 GAAGAC GAAGAC(2/6)
BsaJI 66 CCTGGG C^C[ACGT][ACGT]GG
BsgI 281 GTGCAG GTGCAG(16/14)
BsiEI 447 CGACCG CG[GA][CT]^CG
BsmAI 304 GAGAC GTCTC(1/5)
BsmBI 304 GAGACG CGTCTC(1/5)
BsmFI 197 GGGAC GGGAC(10/14)
Bsp1407I 165 TGTACA T^GTACA
BspMI 431 ACCTGC ACCTGC(4/8)
BsrGI 165 TGTACA T^GTACA
BstUI 445 CGCG CG^CG
BstYI 461 AGATCC [GA]^GATC[CT]
CelII 473 GCTCAGC GC^T[ACGT]AGC
CfoI 21 GCGC GCG^C
DpnII 462 GATC ^GATC
DraI 181 TTTAAA TTT^AAA
FspI 20 TGCGCA TGC^GCA
HhaI 21 GCGC GCG^C
HinP1I 21 GCGC G^CGC
HinfI 452 GAGTC G^A[ACGT]TC
HphI 51 GGTGA GGTGA(8/7)
MaeI 319 CTAG C^TAG
MboI 462 GATC ^GATC
MflI 461 AGATCC [GA]^GATC[CT]
MseI 182 TTAA T^TAA
MvnI 445 CGCG CG^CG
NdeII 462 GATC ^GATC
NlaIII 507 CATG CATG^
PflFI 448 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 452 GAGTC GAGTC(4/5)
RsaI 166 GTAC GT^AC
Sau3AI 462 GATC ^GATC
SfaNI 112 GCATC GCATC(5/9)
SspBI 165 TGTACA T^GTACA
TaqI 275 TCGA T^CGA
Tru9I 182 TTAA T^TAA
Tth111I 448 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 275 TCGA T^CGA
XhoII 461 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
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AciI 444 CCGC CCGC(-3/-1)
322 GCGG
AluI 107 AGCT AG^CT
477 AGCT
AspHI 35 GTGCAC G[AT]GC[AT]^C
361 GTGCTC
BbvI 105 GCAGC GCAGC(8/12)
397 GCAGC
BmyI 35 GTGCAC G[AGT]GC[ACT]^C
361 GTGCTC
BsiHKAI 35 GTGCAC G[AT]GC[AT]^C
361 GTGCTC
BsiYI 66 CCTGGGCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
139 CCTACACAGGG
BslI 66 CCTGGGCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
139 CCTACACAGGG
Bsp1286I 35 GTGCAC G[AGT]GC[ACT]^C
361 GTGCTC
BssKI 45 CCAGG ^CC[ACGT]GG
66 CCTGG
BstAPI 381 GCAGGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
397 GCAGCCCCTGC
BstF5I 441 CATCC GGATG(2/0)
501 GGATG
BstNI 45 CCAGG CC^[AT]GG
66 CCTGG
DdeI 346 CTAAG C^T[ACGT]AG
474 CTCAG
EarI 95 CTCTTC CTCTTC(1/4)
301 GAAGAG
EcoRII 45 CCAGG ^CC[AT]GG
66 CCTGG
Fnu4HI 105 GCAGC GC^[ACGT]GC
397 GCAGC
FokI 441 CATCC GGATG(9/13)
501 GGATG
ItaI 105 GCAGC GC^[ACGT]GC
397 GCAGC
Ksp632I 95 CTCTTC CTCTTC(1/4)
301 GAAGAG
MvaI 45 CCAGG CC^[AT]GG
66 CCTGG
MwoI 381 GCAGGATTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
397 GCAGCCCCTGC
ScrFI 45 CCAGG CC^[ACGT]GG
66 CCTGG
TseI 105 GCAGC G^C[AT]GC
397 GCAGC
TspRI 279 CAGTG CA[GC]TG[ACGT][ACGT]^
359 CAGTG
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# 3 OR MORE CUT ENZYMES ( 5 enzymes) #
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BsrI Cac8I MboII MnlI Tsp509I