################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FbaI FseI FspI HaeII HapII Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MfeI MluI MroI MslI MspI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspBI SspI StuI SwaI TspRI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AfaI 830 GTAC GT^AC AlwI 214 GGATC GGATC(4/5) AvaI 170 CCCGAG C^[CT]CG[GA]G AvaII 38 GGACC G^G[AT]CC BalI 701 TGGCCA TGG^CCA BbsI 256 GAAGAC GAAGAC(2/6) BcgI 657 CGATATTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 740 CTAG C^TAG BpuAI 256 GAAGAC GAAGAC(2/6) BsaBI 209 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaJI 767 CCATGG C^C[ACGT][ACGT]GG BsmAI 99 GAGAC GTCTC(1/5) BsoBI 170 CCCGAG C^[CT]CG[GA]G BsrI 441 CCAGT ACTGG(1/-1) BstAPI 160 GCAAAATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 65 TTCGAA TT^CGAA BstYI 214 GGATCT [GA]^GATC[CT] Cac8I 778 GCCAGC GC[ACGT]^[ACGT]GC CfoI 11 GCGC GCG^C DraII 249 GGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 767 CCATGG C^C[GA][CT]GG EaeI 701 TGGCCA [CT]^GGCC[GA] EcoO109I 249 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoT14I 767 CCATGG C^C[AT][AT]GG HgaI 496 GCGTC GACGC(5/10) HhaI 11 GCGC GCG^C HinP1I 11 GCGC G^CGC MaeI 740 CTAG C^TAG MaeII 671 ACGT A^CGT MaeIII 401 GTGAC ^GT[ACGT]AC MamI 209 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 214 GGATCT [GA]^GATC[CT] MluNI 701 TGGCCA TGG^CCA MscI 701 TGGCCA TGG^CCA MspA1I 368 CAGCTG C[AC]G^C[GT]G NcoI 767 CCATGG C^CATGG NspV 65 TTCGAA TT^CGAA PflMI 441 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 101 GACTAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 881 CTGCAG CTGCA^G PvuII 368 CAGCTG CAG^CTG RsaI 830 GTAC GT^AC SfcI 881 CTGCAG C^T[GA][CT]AG SfuI 65 TTCGAA TT^CGAA SpeI 739 ACTAGT A^CTAGT StyI 767 CCATGG C^C[AT][AT]GG TfiI 57 GATTC G^A[AT]TC Tsp45I 401 GTGAC ^GT[GC]AC Van91I 441 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 214 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 10 CGCG CG^CG 12 CGCG AlwNI 365 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 442 CAGTTTCTG BsiYI 441 CCAGTTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 481 CCCACTTTTGG BslI 441 CCAGTTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 481 CCCACTTTTGG BsmI 786 GAATGC GAATGC(1/-1) 454 GCATTC BstF5I 120 CATCC GGATG(2/0) 758 GGATG BstUI 10 CGCG CG^CG 12 CGCG Cfr13I 38 GGACC G^G[ACGT]CC 250 GGGCC DpnII 215 GATC ^GATC 295 GATC EcoRV 88 GATATC GAT^ATC 241 GATATC FauI 833 CCCGC CCCGC(4/6) 13 GCGGG FokI 120 CATCC GGATG(9/13) 758 GGATG HphI 400 GGTGA GGTGA(8/7) 92 TCACC MboI 215 GATC ^GATC 295 GATC MseI 560 TTAA T^TAA 617 TTAA MvnI 10 CGCG CG^CG 12 CGCG MwoI 13 GCGGGTGTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 160 GCAAAATATGC NdeII 215 GATC ^GATC 295 GATC NlaIII 642 CATG CATG^ 768 CATG NlaIV 249 GGGGCC GG[ACGT]^[ACGT]CC 547 GGCTCC PleI 429 GACTC GAGTC(4/5) 730 GAGTC Sau3AI 215 GATC ^GATC 295 GATC Sau96I 38 GGACC G^G[ACGT]CC 250 GGGCC SfaNI 493 GATGC GCATC(5/9) 664 GATGC Tru9I 560 TTAA T^TAA 617 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AciI AcsI AluI ApoI BbvI BsrDI BssKI BstNI DdeI EcoRII Fnu4HI HaeIII HinfI ItaI MboII MnlI MvaI ScrFI TaqI TseI Tsp509I TthHB8I