(Circular) (Six-base) MAPSORT of: pFN21AE4303.seq Check: 8054 from: 1 to: 5599 pFN21AE4303 5599 bp With 182 enzymes: SgfI * March 28, 2012 14:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3409 278 Size: 53 83 186 936 1873 2468 Fragments arranged by size: 2468 1873 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2942 1937 Size: 1005 4594 Acc65I G'GTAC_C Cuts at: 2920 2920 Size: 5599 AclI AA'CG_TT Cuts at: 1563 3726 4099 1563 Size: 2163 373 3063 Fragments arranged by size: 3063 2163 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2280 2750 3662 5046 5314 1632 Size: 648 470 912 1384 268 1917 Fragments arranged by size: 1917 1384 912 648 470 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5402 AflIII A'CryG_T Cuts at: 1203 4498 5475 1203 Size: 3295 977 1327 Fragments arranged by size: 3295 1327 977 AgeI A'CCGG_T Cuts at: 4430 4430 Size: 5599 AhdI GACnn_n'nnGTC Cuts at: 4328 4328 Size: 5599 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5599 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5599 AlwNI CAG_nnn'CTG Cuts at: 2375 2452 3342 4914 2375 Size: 77 890 1572 3060 Fragments arranged by size: 3060 1572 890 77 ApaLI G'TGCA_C Cuts at: 3656 4812 3656 Size: 1156 4443 ApoI r'AATT_y Cuts at: 1493 2527 2540 2570 2908 3134 3286 5395 1493 Size: 1034 13 30 338 226 152 2109 1697 Fragments arranged by size: 2109 1697 1034 338 226 152 30 13 AseI AT'TA_AT Cuts at: 160 1032 4153 160 Size: 872 3121 1606 Fragments arranged by size: 3121 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5599 AvaI C'yCGr_G Cuts at: 1870 1943 2175 2924 1870 Size: 73 232 749 4545 Fragments arranged by size: 4545 749 232 73 BamHI G'GATC_C Cuts at: 2929 3296 2929 Size: 367 5232 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2920 4375 618 Size: 325 217 627 1133 1455 1842 Fragments arranged by size: 1842 1455 1133 627 325 217 BanII G_rGCy'C Cuts at: 1391 2918 1391 Size: 1527 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2268 928 Size: 971 369 4259 Fragments arranged by size: 4259 971 369 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2651 2685 3790 3824 1903 Size: 34 714 34 1105 34 3678 Fragments arranged by size: 3678 1105 714 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3491 4701 3491 Size: 1210 4389 BclI T'GATC_A Cuts at: 1548 1548 Size: 5599 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4210 136 Size: 107 122 71 631 605 2538 1525 Fragments arranged by size: 2538 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5594 5594 Size: 5599 BlpI GC'TnA_GC Cuts at: 3016 3016 Size: 5599 Bme1580I G_kGCm'C Cuts at: 1790 3660 4816 1790 Size: 1870 1156 2573 Fragments arranged by size: 2573 1870 1156 BmgBI CAC'GTC Cuts at: 1318 2677 1318 Size: 1359 4240 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3330 4288 442 Size: 416 53 83 399 24 1913 958 1753 Fragments arranged by size: 1913 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5599 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4259 1239 Size: 3020 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3724 4589 4887 3724 Size: 865 298 4436 Fragments arranged by size: 4436 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4262 882 Size: 617 2763 2219 Fragments arranged by size: 2763 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3390 493 Size: 711 2186 2702 Fragments arranged by size: 2702 2186 711 BsaBI GATnn'nnATC Cuts at: 2218 5593 2218 Size: 3375 2224 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3406 3788 4423 275 Size: 53 83 186 936 1873 382 635 1451 Fragments arranged by size: 1873 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4032 4430 4704 4851 5262 4032 Size: 398 274 147 411 4369 Fragments arranged by size: 4369 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5374 Fragments arranged by size: 5374 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5599 BseYI C'CCAG_C Cuts at: 2027 2375 4802 5298 2027 Size: 348 2427 496 2328 Fragments arranged by size: 2427 2328 496 348 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5026 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3049 3810 4838 1540 Size: 275 186 1048 761 1028 2301 Fragments arranged by size: 2301 1048 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3660 3745 4816 3660 Size: 85 1071 4443 Fragments arranged by size: 4443 1071 85 BsmI GAATG_Cn' Cuts at: 2459 2797 3110 3203 2459 Size: 338 313 93 4855 Fragments arranged by size: 4855 338 313 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2918 3660 3745 4816 1391 Size: 399 1128 742 85 1071 2174 Fragments arranged by size: 2174 1128 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5262 5262 Size: 5599 BspHI T'CATG_A Cuts at: 3488 5218 3488 Size: 1730 3869 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2954 844 Size: 2110 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3486 1394 Size: 429 1663 3507 Fragments arranged by size: 3507 1663 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2063 2331 2508 4094 4268 65 Size: 1183 815 268 177 1586 174 1396 Fragments arranged by size: 1586 1396 1183 815 268 177 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4243 4430 1700 Size: 110 2433 187 2869 Fragments arranged by size: 2869 2433 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5599 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5599 BssSI C'ACGA_G Cuts at: 3659 4671 3659 Size: 1012 4587 BstAPI GCAn_nnn'nTGC Cuts at: 794 2171 794 Size: 1377 4222 BstBI TT'CG_AA Cuts at: 2071 4403 2071 Size: 2332 3267 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5599 BstYI r'GATC_y Cuts at: 1972 2219 2929 3296 3681 3698 5150 5236 5248 Size: 247 710 367 385 17 1452 86 12 Cuts at: 5248 5594 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 710 385 367 346 247 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2772 513 Size: 552 1707 3340 Fragments arranged by size: 3340 1707 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4860 Fragments arranged by size: 4860 683 56 BtsI GCAGTG_nn' Cuts at: 3115 3909 3929 3115 Size: 794 20 4785 Fragments arranged by size: 4785 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5599 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5564 DraI TTT'AAA Cuts at: 2903 2903 Size: 5599 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4606 809 Size: 507 3290 1802 Fragments arranged by size: 3290 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2706 3046 3935 Size: 54 1425 50 275 18 876 340 889 Cuts at: 3935 5564 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 876 340 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3046 1537 Size: 275 1234 4090 Fragments arranged by size: 4090 1234 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3529 1353 Size: 2176 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4186 4560 4706 387 Size: 1064 138 2597 374 146 1280 Fragments arranged by size: 2597 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2280 2750 3662 4259 5046 5314 1239 Size: 393 648 470 912 597 787 268 1524 Fragments arranged by size: 1524 912 787 648 597 470 393 268 EcoO109I rG'GnC_Cy Cuts at: 2255 2255 Size: 5599 EcoRI G'AATT_C Cuts at: 2908 3286 2908 Size: 378 5221 EcoRV GAT'ATC Cuts at: 2095 2248 2095 Size: 153 5446 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2408 2629 2408 Size: 221 5378 FspI TGC'GCA Cuts at: 4105 4105 Size: 5599 HaeII r_GCGC'y Cuts at: 1678 4746 1678 Size: 3068 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2537 2569 4240 4272 4314 Size: 32 135 32 965 32 1671 32 42 Cuts at: 4314 4346 1373 Size: 32 2626 Fragments arranged by size: 2626 1671 965 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2943 3189 669 Size: 988 281 1005 246 3079 Fragments arranged by size: 3079 1005 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5599 HpaI GTT'AAC Cuts at: 3189 3189 Size: 5599 KpnI G_GTAC'C Cuts at: 2924 2924 Size: 5599 MfeI C'AATT_G Cuts at: 3198 3198 Size: 5599 MluI A'CGCG_T Cuts at: 5475 5475 Size: 5599 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2377 4713 4897 1305 Size: 1072 2336 184 2007 Fragments arranged by size: 2336 2007 1072 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2708 10 Size: 54 1425 343 876 2901 Fragments arranged by size: 2901 1425 876 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3557 3916 4075 518 Size: 648 2391 359 159 2042 Fragments arranged by size: 2391 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2375 3015 3692 4840 5085 1606 Size: 769 640 677 1148 245 2120 Fragments arranged by size: 2120 1148 769 677 640 245 NcoI C'CATG_G Cuts at: 513 1065 2772 513 Size: 552 1707 3340 Fragments arranged by size: 3340 1707 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5599 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5599 NotI GC'GGCC_GC Cuts at: 3046 3046 Size: 5599 NspI r_CATG'y Cuts at: 1132 1229 2957 4502 1132 Size: 97 1728 1545 2229 Fragments arranged by size: 2229 1728 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5599 PciI A'CATG_T Cuts at: 4498 4498 Size: 5599 PflMI CCAn_nnn'nTGG Cuts at: 1108 2452 1108 Size: 1344 4255 PmeI GTTT'AAAC Cuts at: 2903 2903 Size: 5599 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3668 3700 5181 5213 3668 Size: 32 1481 32 4054 Fragments arranged by size: 4054 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2110 1613 Size: 497 5102 PsiI TTA'TAA Cuts at: 3169 3169 Size: 5599 PstI C_TGCA'G Cuts at: 830 2890 2951 830 Size: 2060 61 3478 Fragments arranged by size: 3478 2060 61 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5599 PvuII CAG'CTG Cuts at: 2375 2375 Size: 5599 SalI G'TCGA_C Cuts at: 1936 2941 1936 Size: 1005 4594 SbfI CC_TGCA'GG Cuts at: 2951 2951 Size: 5599 ScaI AGT'ACT Cuts at: 3847 5559 3847 Size: 1712 3887 SfcI C'TryA_G Cuts at: 826 1045 2886 2947 4082 4763 4954 826 Size: 219 1841 61 1135 681 191 1471 Fragments arranged by size: 1841 1471 1135 681 219 191 61 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5599 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5599 SmaI CCC'GGG Cuts at: 1872 2926 1872 Size: 1054 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3703 4604 4866 820 Size: 197 926 1760 901 262 1553 Fragments arranged by size: 1760 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3390 493 Size: 2897 2702 SpeI A'CTAG_T Cuts at: 152 2744 152 Size: 2592 3007 SphI G_CATG'C Cuts at: 1132 2957 1132 Size: 1825 3774 SspI AAT'ATT Cuts at: 5 52 3523 5 Size: 47 3471 2081 Fragments arranged by size: 3471 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5599 StyI C'CwwG_G Cuts at: 513 1065 2772 3038 513 Size: 552 1707 266 3074 Fragments arranged by size: 3074 1707 552 266 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3626 3643 3796 3981 4426 325 Size: 1255 2046 17 153 185 445 1498 Fragments arranged by size: 2046 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3845 5557 96 Size: 274 80 33 51 1443 1868 1712 138 Fragments arranged by size: 1868 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5559 5591 5559 Size: 32 5567 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5599 XbaI T'CTAG_A Cuts at: 2935 2935 Size: 5599 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5599 XmaI C'CCGG_G Cuts at: 1870 2924 1870 Size: 1054 4545 XmnI GAAnn'nnTTC Cuts at: 3728 3728 Size: 5599 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3407 276 Size: 53 83 186 936 1873 2468 Fragments arranged by size: 2468 1873 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI