(Circular) (Six-base) MAPSORT of: pFN21AB3906.seq Check: 6668 from: 1 to: 5959 pFN21AB3906 5959 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3769 278 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3302 1937 Size: 1365 4594 Acc65I G'GTAC_C Cuts at: 3280 3280 Size: 5959 AclI AA'CG_TT Cuts at: 1563 4086 4459 1563 Size: 2523 373 3063 Fragments arranged by size: 3063 2523 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2086 4022 5406 5674 1632 Size: 454 1936 1384 268 1917 Fragments arranged by size: 1936 1917 1384 454 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5762 AflIII A'CryG_T Cuts at: 1203 4858 5835 1203 Size: 3655 977 1327 Fragments arranged by size: 3655 1327 977 AgeI A'CCGG_T Cuts at: 4790 4790 Size: 5959 AhdI GACnn_n'nnGTC Cuts at: 4688 4688 Size: 5959 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5959 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2377 2774 1184 Size: 1193 397 4369 Fragments arranged by size: 4369 1193 397 AlwNI CAG_nnn'CTG Cuts at: 3702 5274 3702 Size: 1572 4387 ApaLI G'TGCA_C Cuts at: 4016 5172 4016 Size: 1156 4803 ApoI r'AATT_y Cuts at: 1493 2549 3268 3494 3646 5755 1493 Size: 1056 719 226 152 2109 1697 Fragments arranged by size: 2109 1697 1056 719 226 152 AseI AT'TA_AT Cuts at: 160 1032 3090 4513 160 Size: 872 2058 1423 1606 Fragments arranged by size: 2058 1606 1423 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 AvaI C'yCGr_G Cuts at: 1870 1943 2058 3284 1870 Size: 73 115 1226 4545 Fragments arranged by size: 4545 1226 115 73 BamHI G'GATC_C Cuts at: 3289 3656 3289 Size: 367 5592 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2016 2054 2081 3280 4735 Size: 325 217 627 229 38 27 1199 1455 Cuts at: 4735 618 Size: 1842 Fragments arranged by size: 1842 1455 1199 627 325 229 217 38 27 BanII G_rGCy'C Cuts at: 1391 2189 2479 3278 1391 Size: 798 290 799 4072 Fragments arranged by size: 4072 799 798 290 BbeI G_GCGC'C Cuts at: 2020 2085 2020 Size: 65 5894 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4988 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4150 4184 1903 Size: 34 50 34 2129 34 3678 Fragments arranged by size: 3678 2129 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3851 5061 3851 Size: 1210 4749 BclI T'GATC_A Cuts at: 1548 1548 Size: 5959 BfrBI ATG'CAT Cuts at: 2008 2008 Size: 5959 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4570 136 Size: 107 122 71 631 605 2898 1525 Fragments arranged by size: 2898 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5954 5954 Size: 5959 BlpI GC'TnA_GC Cuts at: 2403 3376 2403 Size: 973 4986 Bme1580I G_kGCm'C Cuts at: 1790 2059 2205 4020 5176 1790 Size: 269 146 1815 1156 2573 Fragments arranged by size: 2573 1815 1156 269 146 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5959 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2137 2182 3690 Size: 416 53 83 399 24 720 45 1508 Cuts at: 3690 4648 442 Size: 958 1753 Fragments arranged by size: 1753 1508 958 720 416 399 83 53 45 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4619 1239 Size: 3380 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4084 4949 5247 4084 Size: 865 298 4796 Fragments arranged by size: 4796 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4622 882 Size: 617 3123 2219 Fragments arranged by size: 3123 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2635 3750 493 Size: 711 1431 1115 2702 Fragments arranged by size: 2702 1431 1115 711 BsaBI GATnn'nnATC Cuts at: 5953 5953 Size: 5959 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2017 2082 3766 4148 Size: 53 83 186 936 484 65 1684 382 Cuts at: 4148 4783 275 Size: 635 1451 Fragments arranged by size: 1684 1451 936 635 484 382 186 83 65 53 BsaWI w'CCGG_w Cuts at: 4392 4790 5064 5211 5622 4392 Size: 398 274 147 411 4729 Fragments arranged by size: 4729 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5734 Fragments arranged by size: 5734 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5959 BseYI C'CCAG_C Cuts at: 5162 5658 5162 Size: 496 5463 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2153 2291 2699 2942 1163 Size: 573 417 138 408 243 4180 Fragments arranged by size: 4180 573 417 408 243 138 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3409 4170 5198 1540 Size: 275 186 1408 761 1028 2301 Fragments arranged by size: 2301 1408 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2912 4020 4105 5176 2912 Size: 1108 85 1071 3695 Fragments arranged by size: 3695 1108 1071 85 BsiWI C'GTAC_G Cuts at: 2220 2220 Size: 5959 BsmI GAATG_Cn' Cuts at: 3470 3563 3470 Size: 93 5866 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2059 2189 2205 2479 2912 3278 4020 Size: 399 269 130 16 274 433 366 742 Cuts at: 4020 4105 5176 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 433 399 366 274 269 130 85 16 BspEI T'CCGG_A Cuts at: 5622 5622 Size: 5959 BspHI T'CATG_A Cuts at: 3848 5578 3848 Size: 1730 4229 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2175 3314 844 Size: 1331 1139 3489 Fragments arranged by size: 3489 1331 1139 BsrBI CCG'CTC Cuts at: 1394 1823 3846 1394 Size: 429 2023 3507 Fragments arranged by size: 3507 2023 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2649 4454 4628 65 Size: 1183 1401 1805 174 1396 Fragments arranged by size: 1805 1401 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4603 4790 1700 Size: 110 2793 187 2869 Fragments arranged by size: 2869 2793 187 110 BsrGI T'GTAC_A Cuts at: 96 2445 96 Size: 2349 3610 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5959 BssSI C'ACGA_G Cuts at: 4019 5031 4019 Size: 1012 4947 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5959 BstBI TT'CG_AA Cuts at: 4763 4763 Size: 5959 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5959 BstYI r'GATC_y Cuts at: 1972 3289 3656 4041 4058 5510 5596 5608 5954 Size: 1317 367 385 17 1452 86 12 346 Cuts at: 5954 1972 Size: 1977 Fragments arranged by size: 1977 1452 1317 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2012 2232 513 Size: 552 947 220 4240 Fragments arranged by size: 4240 947 552 220 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5220 Fragments arranged by size: 5220 683 56 BtsI GCAGTG_nn' Cuts at: 2272 2290 3194 3475 4269 4289 2272 Size: 18 904 281 794 20 3942 Fragments arranged by size: 3942 904 794 281 20 18 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5959 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5924 DraI TTT'AAA Cuts at: 3263 3263 Size: 5959 DraIII CAC_nnn'GTG Cuts at: 2131 2131 Size: 5959 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2328 4966 809 Size: 507 1012 2638 1802 Fragments arranged by size: 2638 1802 1012 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3406 4295 5924 Size: 54 1425 50 275 18 1576 889 1629 Cuts at: 5924 8 Size: 43 Fragments arranged by size: 1629 1576 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3406 1537 Size: 275 1594 4090 Fragments arranged by size: 4090 1594 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2177 2734 3889 1353 Size: 824 557 1155 3423 Fragments arranged by size: 3423 1155 824 557 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4546 4920 5066 387 Size: 1064 138 2957 374 146 1280 Fragments arranged by size: 2957 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2086 4022 4619 5406 5674 1239 Size: 393 454 1936 597 787 268 1524 Fragments arranged by size: 1936 1524 787 597 454 393 268 EcoO109I rG'GnC_Cy Cuts at: 2097 2228 2468 2097 Size: 131 240 5588 Fragments arranged by size: 5588 240 131 EcoRI G'AATT_C Cuts at: 3268 3646 3268 Size: 378 5581 EcoRV GAT'ATC Cuts at: 2149 2149 Size: 5959 FspI TGC'GCA Cuts at: 4465 4465 Size: 5959 HaeII r_GCGC'y Cuts at: 1678 2020 2085 5106 1678 Size: 342 65 3021 2531 Fragments arranged by size: 3021 2531 342 65 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3105 3137 4600 4632 4674 Size: 32 135 32 1533 32 1463 32 42 Cuts at: 4674 4706 1373 Size: 32 2626 Fragments arranged by size: 2626 1533 1463 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3303 3549 669 Size: 988 281 1365 246 3079 Fragments arranged by size: 3079 1365 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5959 HpaI GTT'AAC Cuts at: 3549 3549 Size: 5959 KasI G'GCGC_C Cuts at: 2016 2081 2016 Size: 65 5894 KpnI G_GTAC'C Cuts at: 3284 3284 Size: 5959 MfeI C'AATT_G Cuts at: 3558 3558 Size: 5959 MluI A'CGCG_T Cuts at: 5835 5835 Size: 5959 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2094 2913 5073 5257 1305 Size: 789 819 2160 184 2007 Fragments arranged by size: 2160 2007 819 789 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4137 Fragments arranged by size: 4137 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2756 3917 4276 4435 518 Size: 648 1590 1161 359 159 2042 Fragments arranged by size: 2042 1590 1161 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2014 2293 3206 3375 4052 5200 5445 1606 Size: 408 279 913 169 677 1148 245 2120 Fragments arranged by size: 2120 1148 913 677 408 279 245 169 NarI GG'CG_CC Cuts at: 2017 2082 2017 Size: 65 5894 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5407 NdeI CA'TA_TG Cuts at: 387 387 Size: 5959 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5959 NotI GC'GGCC_GC Cuts at: 3406 3406 Size: 5959 NsiI A_TGCA'T Cuts at: 2010 2010 Size: 5959 NspI r_CATG'y Cuts at: 1132 1229 3203 3317 4862 1132 Size: 97 1974 114 1545 2229 Fragments arranged by size: 2229 1974 1545 114 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5959 PciI A'CATG_T Cuts at: 4858 4858 Size: 5959 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5959 PmeI GTTT'AAAC Cuts at: 3263 3263 Size: 5959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4028 4060 5541 5573 4028 Size: 32 1481 32 4414 Fragments arranged by size: 4414 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2097 2468 2097 Size: 371 5588 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5959 PsiI TTA'TAA Cuts at: 3162 3182 3529 3162 Size: 20 347 5592 Fragments arranged by size: 5592 347 20 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2598 2630 2598 Size: 32 5927 PstI C_TGCA'G Cuts at: 830 3311 830 Size: 2481 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5959 PvuII CAG'CTG Cuts at: 2293 3206 2293 Size: 913 5046 SacII CC_GC'GG Cuts at: 2015 2015 Size: 5959 SalI G'TCGA_C Cuts at: 1936 3301 1936 Size: 1365 4594 SapI GCTCTTCn'nnn_ Cuts at: 2177 2177 Size: 5959 SbfI CC_TGCA'GG Cuts at: 3311 3311 Size: 5959 ScaI AGT'ACT Cuts at: 4207 5919 4207 Size: 1712 4247 SfcI C'TryA_G Cuts at: 826 1045 3307 4442 5123 5314 826 Size: 219 2262 1135 681 191 1471 Fragments arranged by size: 2262 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2018 2083 2018 Size: 65 5894 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5959 SmaI CCC'GGG Cuts at: 1872 2060 3286 1872 Size: 188 1226 4545 Fragments arranged by size: 4545 1226 188 SmlI C'TyrA_G Cuts at: 820 1017 1943 4063 4964 5226 820 Size: 197 926 2120 901 262 1553 Fragments arranged by size: 2120 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3750 493 Size: 3257 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5959 SphI G_CATG'C Cuts at: 1132 3203 3317 1132 Size: 2071 114 3774 Fragments arranged by size: 3774 2071 114 SspI AAT'ATT Cuts at: 5 52 2440 3883 5 Size: 47 2388 1443 2081 Fragments arranged by size: 2388 2081 1443 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5959 StyI C'CwwG_G Cuts at: 513 1065 2564 3067 3398 513 Size: 552 1499 503 331 3074 Fragments arranged by size: 3074 1499 552 503 331 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2119 3986 4003 4156 4341 4786 325 Size: 1255 539 1867 17 153 185 445 1498 Fragments arranged by size: 1867 1498 1255 539 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2445 2498 3189 Size: 274 80 33 51 1443 468 53 691 Cuts at: 3189 4205 5917 96 Size: 1016 1712 138 Fragments arranged by size: 1712 1443 1016 691 468 274 138 80 53 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5919 5951 5919 Size: 32 5927 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5959 XbaI T'CTAG_A Cuts at: 3295 3295 Size: 5959 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5959 XmaI C'CCGG_G Cuts at: 1870 2058 3284 1870 Size: 188 1226 4545 Fragments arranged by size: 4545 1226 188 XmnI GAAnn'nnTTC Cuts at: 2610 3081 4088 2610 Size: 471 1007 4481 Fragments arranged by size: 4481 1007 471 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3767 276 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI RsrII SacI SanDI SexAI SfiI SrfI SwaI XcmI