Restriction map for
pFN21AB3906
(Linear) (Six-base) MAPSORT of: KIBB3906.seq Check: 3577 from: 1 to: 1254
KIBB3906.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 1254
Size: 81 1173
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 372 769 1254
Size: 372 397 485
Fragments arranged by size:
485 397 372
ApoI r'AATT_y
Cuts at: 0 544 1254
Size: 544 710
AseI AT'TA_AT
Cuts at: 0 1085 1254
Size: 1085 169
AvaI C'yCGr_G
Cuts at: 0 53 1254
Size: 53 1201
BanI G'GyrC_C
Cuts at: 0 11 49 76 1254
Size: 11 38 27 1178
Fragments arranged by size:
1178 38 27 11
BanII G_rGCy'C
Cuts at: 0 184 474 1254
Size: 184 290 780
Fragments arranged by size:
780 290 184
BbeI G_GCGC'C
Cuts at: 0 15 80 1254
Size: 15 65 1174
Fragments arranged by size:
1174 65 15
BfrBI ATG'CAT
Cuts at: 0 3 1254
Size: 3 1251
BlpI GC'TnA_GC
Cuts at: 0 398 1254
Size: 398 856
Bme1580I G_kGCm'C
Cuts at: 0 54 200 1254
Size: 54 146 1054
Fragments arranged by size:
1054 146 54
BmrI ACTGGGnnnn_n'
Cuts at: 0 132 177 1254
Size: 132 45 1077
Fragments arranged by size:
1077 132 45
BsaAI yAC'GTr
Cuts at: 0 630 1254
Size: 630 624
BsaHI Gr'CG_yC
Cuts at: 0 12 77 1254
Size: 12 65 1177
Fragments arranged by size:
1177 65 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 286 694 937 1254
Size: 148 138 408 243 317
Fragments arranged by size:
408 317 243 148 138
BsiHKAI G_wGCw'C
Cuts at: 0 907 1254
Size: 907 347
BsiWI C'GTAC_G
Cuts at: 0 215 1254
Size: 215 1039
Bsp1286I G_dGCh'C
Cuts at: 0 54 184 200 474 907 1254
Size: 54 130 16 274 433 347
Fragments arranged by size:
433 347 274 130 54 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 170 1254
Size: 170 1084
BsrDI GCAATG_nn'
Cuts at: 0 644 1254
Size: 644 610
BsrGI T'GTAC_A
Cuts at: 0 440 1254
Size: 440 814
BtgI C'CryG_G
Cuts at: 0 7 227 1254
Size: 7 220 1027
Fragments arranged by size:
1027 220 7
BtsI GCAGTG_nn'
Cuts at: 0 267 285 1189 1254
Size: 267 18 904 65
Fragments arranged by size:
904 267 65 18
DraIII CAC_nnn'GTG
Cuts at: 0 126 1254
Size: 126 1128
DrdI GACnn_nn'nnGTC
Cuts at: 0 323 1254
Size: 323 931
EarI CTCTTCn'nnn_
Cuts at: 0 172 729 1254
Size: 172 557 525
Fragments arranged by size:
557 525 172
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 1254
Size: 81 1173
EcoO109I rG'GnC_Cy
Cuts at: 0 92 223 463 1254
Size: 92 131 240 791
Fragments arranged by size:
791 240 131 92
EcoRV GAT'ATC
Cuts at: 0 144 1254
Size: 144 1110
HaeII r_GCGC'y
Cuts at: 0 15 80 1254
Size: 15 65 1174
Fragments arranged by size:
1174 65 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1100 1132 1254
Size: 1100 32 122
Fragments arranged by size:
1100 122 32
KasI G'GCGC_C
Cuts at: 0 11 76 1254
Size: 11 65 1178
Fragments arranged by size:
1178 65 11
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 908 1254
Size: 89 819 346
Fragments arranged by size:
819 346 89
MslI CAynn'nnrTG
Cuts at: 0 751 1254
Size: 751 503
MspA1I CmG'CkG
Cuts at: 0 9 288 1201 1254
Size: 9 279 913 53
Fragments arranged by size:
913 279 53 9
NarI GG'CG_CC
Cuts at: 0 12 77 1254
Size: 12 65 1177
Fragments arranged by size:
1177 65 12
NsiI A_TGCA'T
Cuts at: 0 5 1254
Size: 5 1249
NspI r_CATG'y
Cuts at: 0 1198 1254
Size: 1198 56
PpuMI rG'GwC_Cy
Cuts at: 0 92 463 1254
Size: 92 371 791
Fragments arranged by size:
791 371 92
PsiI TTA'TAA
Cuts at: 0 1157 1177 1254
Size: 1157 20 77
Fragments arranged by size:
1157 77 20
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 593 625 1254
Size: 593 32 629
Fragments arranged by size:
629 593 32
PvuII CAG'CTG
Cuts at: 0 288 1201 1254
Size: 288 913 53
Fragments arranged by size:
913 288 53
SacII CC_GC'GG
Cuts at: 0 10 1254
Size: 10 1244
SapI GCTCTTCn'nnn_
Cuts at: 0 172 1254
Size: 172 1082
SfoI GGC'GCC
Cuts at: 0 13 78 1254
Size: 13 65 1176
Fragments arranged by size:
1176 65 13
SmaI CCC'GGG
Cuts at: 0 55 1254
Size: 55 1199
SphI G_CATG'C
Cuts at: 0 1198 1254
Size: 1198 56
SspI AAT'ATT
Cuts at: 0 435 1254
Size: 435 819
StyI C'CwwG_G
Cuts at: 0 559 1062 1254
Size: 559 503 192
Fragments arranged by size:
559 503 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 114 1254
Size: 114 1140
TatI w'GTAC_w
Cuts at: 0 440 493 1184 1254
Size: 440 53 691 70
Fragments arranged by size:
691 440 70 53
XmaI C'CCGG_G
Cuts at: 0 53 1254
Size: 53 1201
XmnI GAAnn'nnTTC
Cuts at: 0 605 1076 1254
Size: 605 471 178
Fragments arranged by size:
605 471 178
Enzymes that do cut:
AcuI AlfI ApoI AseI AvaI BanI BanII BbeI
BfrBI BlpI Bme1580I BmrI BsaAI BsaHI BsgI BsiHKAI
BsiWI Bsp1286I BspMI BsrDI BsrGI BtgI BtsI DraIII
DrdI EarI Eco57MI EcoO109I EcoRV HaeII Hin4I KasI
MmeI MslI MspA1I NarI NsiI NspI PpuMI PsiI
PsrI PvuII SacII SapI SfoI SmaI SphI SspI
StyI TaqII TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaI ApaLI AscI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BglI BglII BmgBI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaBI BsaWI BsaXI BseRI BseYI BsiEI
BsmI BsmBI BspEI BspHI BsrBI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI EaeI EagI EciI EcoICRI EcoNI
EcoRI FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MscI NaeI NcoI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PspOMI PspXI PstI PvuI
RsrII SacI SalI SanDI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI StuI
SwaI TstI Tth111I XbaI XcmI XhoI ZraI