(Circular) (Six-base) MAPSORT of: pFN21AB3821.seq Check: 364 from: 1 to: 5974 pFN21AB3821 5974 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3784 278 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3317 1937 Size: 1380 4594 Acc65I G'GTAC_C Cuts at: 3295 3295 Size: 5974 AclI AA'CG_TT Cuts at: 1563 4101 4474 1563 Size: 2538 373 3063 Fragments arranged by size: 3063 2538 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4037 5421 5689 1632 Size: 2405 1384 268 1917 Fragments arranged by size: 2405 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5777 AflIII A'CryG_T Cuts at: 1203 4873 5850 1203 Size: 3670 977 1327 Fragments arranged by size: 3670 1327 977 AgeI A'CCGG_T Cuts at: 4805 4805 Size: 5974 AhdI GACnn_n'nnGTC Cuts at: 4703 4703 Size: 5974 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5974 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5974 AlwNI CAG_nnn'CTG Cuts at: 2085 3271 3717 5289 2085 Size: 1186 446 1572 2770 Fragments arranged by size: 2770 1572 1186 446 ApaLI G'TGCA_C Cuts at: 4031 5187 4031 Size: 1156 4818 ApoI r'AATT_y Cuts at: 1493 3283 3509 3661 5770 1493 Size: 1790 226 152 2109 1697 Fragments arranged by size: 2109 1790 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2219 4528 160 Size: 872 1187 2309 1606 Fragments arranged by size: 2309 1606 1187 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 AvaI C'yCGr_G Cuts at: 1870 1943 3299 1870 Size: 73 1356 4545 Fragments arranged by size: 4545 1356 73 BamHI G'GATC_C Cuts at: 2390 3304 3671 2390 Size: 914 367 4693 Fragments arranged by size: 4693 914 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2326 3295 4750 618 Size: 325 217 627 539 969 1455 1842 Fragments arranged by size: 1842 1455 969 627 539 325 217 BanII G_rGCy'C Cuts at: 1391 3293 1391 Size: 1902 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5003 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4165 4199 1903 Size: 34 50 34 2144 34 3678 Fragments arranged by size: 3678 2144 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2586 3866 5076 2586 Size: 1280 1210 3484 Fragments arranged by size: 3484 1280 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5974 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4585 136 Size: 107 122 71 631 605 2913 1525 Fragments arranged by size: 2913 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5969 5969 Size: 5974 BlpI GC'TnA_GC Cuts at: 3391 3391 Size: 5974 Bme1580I G_kGCm'C Cuts at: 1790 4035 5191 1790 Size: 2245 1156 2573 Fragments arranged by size: 2573 2245 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5974 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3705 4663 442 Size: 416 53 83 399 24 2288 958 1753 Fragments arranged by size: 2288 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5974 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2107 2897 4634 1239 Size: 868 790 1737 2579 Fragments arranged by size: 2579 1737 868 790 Bpu10I CC'TnA_GC Cuts at: 3206 3206 Size: 5974 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2163 2774 3048 4099 4964 5262 2163 Size: 611 274 1051 865 298 2875 Fragments arranged by size: 2875 1051 865 611 298 274 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2286 4637 882 Size: 617 787 2351 2219 Fragments arranged by size: 2351 2219 787 617 BsaAI yAC'GTr Cuts at: 493 1204 3765 493 Size: 711 2561 2702 Fragments arranged by size: 2702 2561 711 BsaBI GATnn'nnATC Cuts at: 5968 5968 Size: 5974 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2052 3781 4163 4798 Size: 53 83 186 936 519 1729 382 635 Cuts at: 4798 275 Size: 1451 Fragments arranged by size: 1729 1451 936 635 519 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4407 4805 5079 5226 5637 4407 Size: 398 274 147 411 4744 Fragments arranged by size: 4744 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2080 2110 2576 2606 2682 Size: 30 165 30 677 30 466 30 76 Cuts at: 2682 2712 1178 Size: 30 4440 Fragments arranged by size: 4440 677 466 165 76 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2690 1186 Size: 1504 4470 BseYI C'CCAG_C Cuts at: 2154 5177 5673 2154 Size: 3023 496 2455 Fragments arranged by size: 3023 2455 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2142 1163 Size: 573 406 4995 Fragments arranged by size: 4995 573 406 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2136 3424 4185 5213 1540 Size: 275 186 135 1288 761 1028 2301 Fragments arranged by size: 2301 1288 1028 761 275 186 135 BsiHKAI G_wGCw'C Cuts at: 2672 4035 4120 5191 2672 Size: 1363 85 1071 3455 Fragments arranged by size: 3455 1363 1071 85 BsmI GAATG_Cn' Cuts at: 3160 3485 3578 3160 Size: 325 93 5556 Fragments arranged by size: 5556 325 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2672 3293 4035 4120 5191 1391 Size: 399 882 621 742 85 1071 2174 Fragments arranged by size: 2174 1071 882 742 621 399 85 BspEI T'CCGG_A Cuts at: 5637 5637 Size: 5974 BspHI T'CATG_A Cuts at: 2298 3863 5593 2298 Size: 1565 1730 2679 Fragments arranged by size: 2679 1730 1565 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3329 844 Size: 2485 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3217 3861 1394 Size: 429 1394 644 3507 Fragments arranged by size: 3507 1394 644 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4469 4643 65 Size: 1183 3221 174 1396 Fragments arranged by size: 3221 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4618 4805 1700 Size: 110 2808 187 2869 Fragments arranged by size: 2869 2808 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5974 BssHII G'CGCG_C Cuts at: 1925 2041 1925 Size: 116 5858 BssSI C'ACGA_G Cuts at: 4034 5046 4034 Size: 1012 4962 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5974 BstBI TT'CG_AA Cuts at: 4778 4778 Size: 5974 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5974 BstXI CCAn_nnnn'nTGG Cuts at: 2109 2109 Size: 5974 BstYI r'GATC_y Cuts at: 1972 2390 3304 3671 4056 4073 5525 5611 5623 Size: 418 914 367 385 17 1452 86 12 Cuts at: 5623 5969 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 914 418 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2835 2835 Size: 5974 BtgI C'CryG_G Cuts at: 513 1065 2046 2102 513 Size: 552 981 56 4385 Fragments arranged by size: 4385 981 552 56 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2320 487 Size: 683 56 1094 4141 Fragments arranged by size: 4141 1094 683 56 BtsI GCAGTG_nn' Cuts at: 2795 3490 4284 4304 2795 Size: 695 794 20 4465 Fragments arranged by size: 4465 794 695 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5974 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3064 3099 529 Size: 35 2500 35 3404 Fragments arranged by size: 3404 2500 35 35 DraI TTT'AAA Cuts at: 3278 3278 Size: 5974 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4981 809 Size: 507 3665 1802 Fragments arranged by size: 3665 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2133 3147 3421 Size: 54 1425 50 275 18 303 1014 274 Cuts at: 3421 4310 5939 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1014 889 303 275 274 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2133 3421 1537 Size: 275 321 1288 4090 Fragments arranged by size: 4090 1288 321 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2189 2285 2523 2753 3097 3904 1353 Size: 836 96 238 230 344 807 3423 Fragments arranged by size: 3423 836 807 344 238 230 96 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2403 4561 4935 5081 387 Size: 1064 138 814 2158 374 146 1280 Fragments arranged by size: 2158 1280 1064 814 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2107 2897 4037 4634 5421 5689 1239 Size: 393 475 790 1140 597 787 268 1524 Fragments arranged by size: 1524 1140 790 787 597 475 393 268 EcoO109I rG'GnC_Cy Cuts at: 2237 2939 3142 2237 Size: 702 203 5069 Fragments arranged by size: 5069 702 203 EcoRI G'AATT_C Cuts at: 3283 3661 3283 Size: 378 5596 FspI TGC'GCA Cuts at: 4480 4480 Size: 5974 HaeII r_GCGC'y Cuts at: 1678 2850 5121 1678 Size: 1172 2271 2531 Fragments arranged by size: 2531 2271 1172 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2080 2112 2382 2414 2438 Size: 32 135 32 508 32 270 32 24 Cuts at: 2438 2470 3233 3265 4615 4647 4689 4721 1373 Size: 32 763 32 1350 32 42 32 2626 Fragments arranged by size: 2626 1350 763 508 270 135 42 32 32 32 32 32 32 32 32 24 HincII GTy'rAC Cuts at: 669 1657 1938 2815 3318 3564 669 Size: 988 281 877 503 246 3079 Fragments arranged by size: 3079 988 877 503 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5974 HpaI GTT'AAC Cuts at: 3564 3564 Size: 5974 KpnI G_GTAC'C Cuts at: 3299 3299 Size: 5974 MfeI C'AATT_G Cuts at: 2523 3573 2523 Size: 1050 4924 MluI A'CGCG_T Cuts at: 5850 5850 Size: 5974 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5088 5272 1305 Size: 3783 184 2007 Fragments arranged by size: 3783 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4152 Fragments arranged by size: 4152 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2107 3932 4291 4450 518 Size: 648 941 1825 359 159 2042 Fragments arranged by size: 2042 1825 941 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2048 2071 2085 2126 2132 2589 3199 3271 Size: 442 23 14 41 6 457 610 72 Cuts at: 3271 3390 4067 5215 5460 1606 Size: 119 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 610 457 442 245 119 72 41 23 14 6 NcoI C'CATG_G Cuts at: 513 1065 2102 513 Size: 552 1037 4385 Fragments arranged by size: 4385 1037 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5974 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5974 NotI GC'GGCC_GC Cuts at: 3421 3421 Size: 5974 NspI r_CATG'y Cuts at: 1132 1229 2162 3332 4877 1132 Size: 97 933 1170 1545 2229 Fragments arranged by size: 2229 1545 1170 933 97 PasI CC'CwG_GG Cuts at: 1348 2164 1348 Size: 816 5158 PciI A'CATG_T Cuts at: 4873 4873 Size: 5974 PflMI CCAn_nnn'nTGG Cuts at: 1108 2085 3271 1108 Size: 977 1186 3811 Fragments arranged by size: 3811 1186 977 PmeI GTTT'AAAC Cuts at: 3278 3278 Size: 5974 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4043 4075 5556 5588 4043 Size: 32 1481 32 4429 Fragments arranged by size: 4429 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2939 2939 Size: 5974 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5974 PsiI TTA'TAA Cuts at: 3544 3544 Size: 5974 PstI C_TGCA'G Cuts at: 830 2131 3239 3326 830 Size: 1301 1108 87 3478 Fragments arranged by size: 3478 1301 1108 87 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5974 PvuII CAG'CTG Cuts at: 2085 2126 2589 3199 3271 2085 Size: 41 463 610 72 4788 Fragments arranged by size: 4788 610 463 72 41 SacII CC_GC'GG Cuts at: 2049 2049 Size: 5974 SalI G'TCGA_C Cuts at: 1936 3316 1936 Size: 1380 4594 SapI GCTCTTCn'nnn_ Cuts at: 3097 3097 Size: 5974 SbfI CC_TGCA'GG Cuts at: 3326 3326 Size: 5974 ScaI AGT'ACT Cuts at: 2882 3059 4222 5934 2882 Size: 177 1163 1712 2922 Fragments arranged by size: 2922 1712 1163 177 SfcI C'TryA_G Cuts at: 826 1045 2127 3235 3322 4457 5138 5329 826 Size: 219 1082 1108 87 1135 681 191 1471 Fragments arranged by size: 1471 1135 1108 1082 681 219 191 87 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5974 SmaI CCC'GGG Cuts at: 1872 3301 1872 Size: 1429 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2178 2753 3063 4078 4979 5241 Size: 197 926 235 575 310 1015 901 262 Cuts at: 5241 820 Size: 1553 Fragments arranged by size: 1553 1015 926 901 575 310 262 235 197 SnaBI TAC'GTA Cuts at: 493 3765 493 Size: 3272 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5974 SphI G_CATG'C Cuts at: 1132 2162 3332 1132 Size: 1030 1170 3774 Fragments arranged by size: 3774 1170 1030 SspI AAT'ATT Cuts at: 5 52 2217 3898 5 Size: 47 2165 1681 2081 Fragments arranged by size: 2165 2081 1681 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5974 StyI C'CwwG_G Cuts at: 513 1065 2102 2913 3075 3413 513 Size: 552 1037 811 162 338 3074 Fragments arranged by size: 3074 1037 811 552 338 162 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2304 4001 4018 4171 4356 4801 325 Size: 1255 724 1697 17 153 185 445 1498 Fragments arranged by size: 1697 1498 1255 724 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2583 2880 3057 Size: 274 80 33 51 1443 606 297 177 Cuts at: 3057 4220 5932 96 Size: 1163 1712 138 Fragments arranged by size: 1712 1443 1163 606 297 274 177 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2104 2136 2560 2592 2682 2714 5934 5966 2104 Size: 32 424 32 90 32 3220 32 2112 Fragments arranged by size: 3220 2112 424 90 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5974 XbaI T'CTAG_A Cuts at: 3310 3310 Size: 5974 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3268 3268 Size: 5974 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5974 XmaI C'CCGG_G Cuts at: 1870 3299 1870 Size: 1429 4545 XmnI GAAnn'nnTTC Cuts at: 4103 4103 Size: 5974 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3782 276 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI