(Circular) (Six-base) MAPSORT of: pFN21AB3815.seq Check: 6480 from: 1 to: 5914 pFN21AB3815 5914 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3121 3121 Size: 5914 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3724 278 Size: 53 83 186 936 2188 2468 Fragments arranged by size: 2468 2188 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2207 2945 3257 1937 Size: 270 738 312 4594 Fragments arranged by size: 4594 738 312 270 Acc65I G'GTAC_C Cuts at: 3235 3235 Size: 5914 AclI AA'CG_TT Cuts at: 1563 4041 4414 1563 Size: 2478 373 3063 Fragments arranged by size: 3063 2478 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3977 5361 5629 1632 Size: 2345 1384 268 1917 Fragments arranged by size: 2345 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5717 AflIII A'CryG_T Cuts at: 1203 4813 5790 1203 Size: 3610 977 1327 Fragments arranged by size: 3610 1327 977 AgeI A'CCGG_T Cuts at: 4745 4745 Size: 5914 AhdI GACnn_n'nnGTC Cuts at: 4643 4643 Size: 5914 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5914 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2163 1184 Size: 979 4935 AlwNI CAG_nnn'CTG Cuts at: 2216 3657 5229 2216 Size: 1441 1572 2901 Fragments arranged by size: 2901 1572 1441 ApaLI G'TGCA_C Cuts at: 3971 5127 3971 Size: 1156 4758 ApoI r'AATT_y Cuts at: 1493 3200 3223 3449 3601 5710 1493 Size: 1707 23 226 152 2109 1697 Fragments arranged by size: 2109 1707 1697 226 152 23 AseI AT'TA_AT Cuts at: 160 1032 4468 160 Size: 872 3436 1606 Fragments arranged by size: 3436 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5914 AvaI C'yCGr_G Cuts at: 1870 1943 3239 1870 Size: 73 1296 4545 Fragments arranged by size: 4545 1296 73 AvrII C'CTAG_G Cuts at: 2566 2566 Size: 5914 BamHI G'GATC_C Cuts at: 2710 3244 3611 2710 Size: 534 367 5013 Fragments arranged by size: 5013 534 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2366 2399 2467 2698 2778 Size: 325 217 627 579 33 68 231 80 Cuts at: 2778 3235 4690 618 Size: 457 1455 1842 Fragments arranged by size: 1842 1455 627 579 457 325 231 217 80 68 33 BanII G_rGCy'C Cuts at: 1391 2046 2174 3233 1391 Size: 655 128 1059 4072 Fragments arranged by size: 4072 1059 655 128 BbeI G_GCGC'C Cuts at: 2782 2782 Size: 5914 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2212 928 Size: 971 313 4630 Fragments arranged by size: 4630 971 313 BbvCI CC'TCA_GC Cuts at: 2637 2637 Size: 5914 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2083 2117 4105 4139 1903 Size: 34 146 34 1988 34 3678 Fragments arranged by size: 3678 1988 146 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3806 5016 3806 Size: 1210 4704 BclI T'GATC_A Cuts at: 1548 1548 Size: 5914 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3042 3161 4525 Size: 107 122 71 631 605 1370 119 1364 Cuts at: 4525 136 Size: 1525 Fragments arranged by size: 1525 1370 1364 631 605 122 119 107 71 BglII A'GATC_T Cuts at: 2499 5909 2499 Size: 3410 2504 BlpI GC'TnA_GC Cuts at: 2045 3189 3331 2045 Size: 1144 142 4628 Fragments arranged by size: 4628 1144 142 Bme1580I G_kGCm'C Cuts at: 1790 2152 2404 2824 3047 3975 5131 1790 Size: 362 252 420 223 928 1156 2573 Fragments arranged by size: 2573 1156 928 420 362 252 223 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5914 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2902 3645 4603 Size: 416 53 83 399 24 1485 743 958 Cuts at: 4603 442 Size: 1753 Fragments arranged by size: 1753 1485 958 743 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5914 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2048 2281 2289 2537 2636 4574 1239 Size: 809 233 8 248 99 1938 2579 Fragments arranged by size: 2579 1938 809 248 233 99 8 Bpu10I CC'TnA_GC Cuts at: 2637 2637 Size: 5914 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2647 3068 4039 4904 5202 2647 Size: 421 971 865 298 3359 Fragments arranged by size: 3359 971 865 421 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2896 4577 882 Size: 617 1397 1681 2219 Fragments arranged by size: 2219 1681 1397 617 BsaAI yAC'GTr Cuts at: 493 1204 3705 493 Size: 711 2501 2702 Fragments arranged by size: 2702 2501 711 BsaBI GATnn'nnATC Cuts at: 2159 5908 2159 Size: 3749 2165 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2779 2960 3721 4103 Size: 53 83 186 936 1246 181 761 382 Cuts at: 4103 4738 275 Size: 635 1451 Fragments arranged by size: 1451 1246 936 761 635 382 186 181 83 53 BsaWI w'CCGG_w Cuts at: 2887 4347 4745 5019 5166 5577 2887 Size: 1460 398 274 147 411 3224 Fragments arranged by size: 3224 1460 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2869 2899 1178 Size: 30 165 30 1466 30 4193 Fragments arranged by size: 4193 1466 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2045 2048 1186 Size: 859 3 5052 Fragments arranged by size: 5052 859 3 BseYI C'CCAG_C Cuts at: 2632 2690 2855 2963 3040 5117 5613 2632 Size: 58 165 108 77 2077 496 2933 Fragments arranged by size: 2933 2077 496 165 108 77 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5341 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3364 4125 5153 1540 Size: 275 186 1363 761 1028 2301 Fragments arranged by size: 2301 1363 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2046 3975 4060 5131 2046 Size: 1929 85 1071 2829 Fragments arranged by size: 2829 1929 1071 85 BsmI GAATG_Cn' Cuts at: 3425 3518 3425 Size: 93 5821 BsmBI CGTCTCn'nnnn_ Cuts at: 2927 2927 Size: 5914 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2152 2174 2404 2824 3047 3233 Size: 399 256 106 22 230 420 223 186 Cuts at: 3233 3975 4060 5131 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 420 399 256 230 223 186 106 85 22 BspEI T'CCGG_A Cuts at: 5577 5577 Size: 5914 BspHI T'CATG_A Cuts at: 3803 5533 3803 Size: 1730 4184 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2433 2991 3121 3269 844 Size: 1589 558 130 148 3489 Fragments arranged by size: 3489 1589 558 148 130 BsrBI CCG'CTC Cuts at: 1394 1823 2010 2863 3193 3801 1394 Size: 429 187 853 330 608 3507 Fragments arranged by size: 3507 853 608 429 330 187 BsrDI GCAATG_nn' Cuts at: 65 1248 4409 4583 65 Size: 1183 3161 174 1396 Fragments arranged by size: 3161 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4558 4745 1700 Size: 110 2748 187 2869 Fragments arranged by size: 2869 2748 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5914 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5914 BssSI C'ACGA_G Cuts at: 3974 4986 3974 Size: 1012 4902 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5914 BstBI TT'CG_AA Cuts at: 4718 4718 Size: 5914 BstEII G'GTnAC_C Cuts at: 1184 2641 1184 Size: 1457 4457 BstXI CCAn_nnnn'nTGG Cuts at: 2902 2902 Size: 5914 BstYI r'GATC_y Cuts at: 1972 2382 2499 2710 2802 3244 3611 3996 4013 Size: 410 117 211 92 442 367 385 17 Cuts at: 4013 5465 5551 5563 5909 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 442 410 385 367 346 211 117 92 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5362 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2881 487 Size: 683 56 1655 3520 Fragments arranged by size: 3520 1655 683 56 BtsI GCAGTG_nn' Cuts at: 2152 2919 3150 3430 4224 4244 2152 Size: 767 231 280 794 20 3822 Fragments arranged by size: 3822 794 767 280 231 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5914 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5879 DraI TTT'AAA Cuts at: 3218 3218 Size: 5914 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2941 4921 809 Size: 507 1625 1980 1802 Fragments arranged by size: 1980 1802 1625 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2937 3361 4250 Size: 54 1425 50 275 18 1107 424 889 Cuts at: 4250 5879 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1107 889 424 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3361 1537 Size: 275 1549 4090 Fragments arranged by size: 4090 1549 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3844 1353 Size: 2491 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4501 4875 5021 387 Size: 1064 138 2912 374 146 1280 Fragments arranged by size: 2912 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2044 2044 Size: 5914 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2281 2289 2537 2636 3977 4574 Size: 393 416 233 8 248 99 1341 597 Cuts at: 4574 5361 5629 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1341 787 597 416 393 268 248 233 99 8 EcoO109I rG'GnC_Cy Cuts at: 2260 2422 2260 Size: 162 5752 EcoRI G'AATT_C Cuts at: 3223 3601 3223 Size: 378 5536 FspI TGC'GCA Cuts at: 4420 4420 Size: 5914 HaeII r_GCGC'y Cuts at: 1678 2782 2856 5061 1678 Size: 1104 74 2205 2531 Fragments arranged by size: 2531 2205 1104 74 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2526 2558 4555 4587 4629 Size: 32 135 32 954 32 1997 32 42 Cuts at: 4629 4661 1373 Size: 32 2626 Fragments arranged by size: 2626 1997 954 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3258 3504 669 Size: 988 281 1320 246 3079 Fragments arranged by size: 3079 1320 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5914 HpaI GTT'AAC Cuts at: 3504 3504 Size: 5914 KasI G'GCGC_C Cuts at: 2778 2778 Size: 5914 KpnI G_GTAC'C Cuts at: 3239 3239 Size: 5914 MfeI C'AATT_G Cuts at: 3513 3513 Size: 5914 MluI A'CGCG_T Cuts at: 5790 5790 Size: 5914 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3093 5028 5212 1305 Size: 1788 1935 184 2007 Fragments arranged by size: 2007 1935 1788 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4092 Fragments arranged by size: 4092 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3872 4231 4390 518 Size: 648 2706 359 159 2042 Fragments arranged by size: 2706 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2216 2636 3330 4007 5155 5400 1606 Size: 610 420 694 677 1148 245 2120 Fragments arranged by size: 2120 1148 694 677 610 420 245 NarI GG'CG_CC Cuts at: 2779 2779 Size: 5914 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5362 NdeI CA'TA_TG Cuts at: 387 387 Size: 5914 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5914 NotI GC'GGCC_GC Cuts at: 3361 3361 Size: 5914 NspI r_CATG'y Cuts at: 1132 1229 2870 3272 4817 1132 Size: 97 1641 402 1545 2229 Fragments arranged by size: 2229 1641 1545 402 97 PasI CC'CwG_GG Cuts at: 1348 3122 1348 Size: 1774 4140 PciI A'CATG_T Cuts at: 4813 4813 Size: 5914 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5914 PfoI T'CCnGG_A Cuts at: 2133 2514 2133 Size: 381 5533 PmeI GTTT'AAAC Cuts at: 3218 3218 Size: 5914 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3983 4015 5496 5528 3983 Size: 32 1481 32 4369 Fragments arranged by size: 4369 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2260 2422 2260 Size: 162 5752 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5914 PsiI TTA'TAA Cuts at: 3484 3484 Size: 5914 PstI C_TGCA'G Cuts at: 830 3266 830 Size: 2436 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5914 PvuII CAG'CTG Cuts at: 2216 2636 2216 Size: 420 5494 SacI G_AGCT'C Cuts at: 2046 2046 Size: 5914 SalI G'TCGA_C Cuts at: 1936 3256 1936 Size: 1320 4594 SbfI CC_TGCA'GG Cuts at: 3266 3266 Size: 5914 ScaI AGT'ACT Cuts at: 4162 5874 4162 Size: 1712 4202 SfcI C'TryA_G Cuts at: 826 1045 2208 3262 4397 5078 5269 826 Size: 219 1163 1054 1135 681 191 1471 Fragments arranged by size: 1471 1163 1135 1054 681 219 191 SfoI GGC'GCC Cuts at: 2780 2780 Size: 5914 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5914 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5914 SmaI CCC'GGG Cuts at: 1872 3241 1872 Size: 1369 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2662 3083 4018 4919 5181 820 Size: 197 926 719 421 935 901 262 1553 Fragments arranged by size: 1553 935 926 901 719 421 262 197 SnaBI TAC'GTA Cuts at: 493 3705 493 Size: 3212 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5914 SphI G_CATG'C Cuts at: 1132 3272 1132 Size: 2140 3774 SspI AAT'ATT Cuts at: 5 52 3838 5 Size: 47 3786 2081 Fragments arranged by size: 3786 2081 47 StuI AGG'CCT Cuts at: 1517 2302 1517 Size: 785 5129 StyI C'CwwG_G Cuts at: 513 1065 2566 3353 513 Size: 552 1501 787 3074 Fragments arranged by size: 3074 1501 787 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2542 2704 3941 3958 4111 4296 4741 Size: 1255 962 162 1237 17 153 185 445 Cuts at: 4741 325 Size: 1498 Fragments arranged by size: 1498 1255 1237 962 445 185 162 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4160 5872 96 Size: 274 80 33 51 1443 2183 1712 138 Fragments arranged by size: 2183 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2869 2901 5874 5906 2869 Size: 32 2973 32 2877 Fragments arranged by size: 2973 2877 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5914 XbaI T'CTAG_A Cuts at: 2929 3250 2929 Size: 321 5593 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2615 2692 3097 2615 Size: 77 405 5432 Fragments arranged by size: 5432 405 77 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5914 XmaI C'CCGG_G Cuts at: 1870 3239 1870 Size: 1369 4545 XmnI GAAnn'nnTTC Cuts at: 4043 4043 Size: 5914 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3722 276 Size: 53 83 186 936 2188 2468 Fragments arranged by size: 2468 2188 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI