Restriction map for
pFN21AB3799
##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccIII AcsI AflII
AgeI AhdI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BbeI BbvCI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspMI BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HinfI HpaI KasI
KpnI MaeI MamI MfeI MroI
MseI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmeI
PshAI PshBI PvuI RsrII SalI
SapI SbfI ScaI SexAI SfoI
SfuI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp509I Tth111I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 1159 GTAGAC GT^[AC][GT]AC
AclI 63 AACGTT AA^CGTT
AcyI 1414 GACGCC G[GA]^CG[CT]C
AocI 162 CCTGAGG CC^T[ACGT]AGG
BalI 1004 TGGCCA TGG^CCA
BamHI 1104 GGATCC G^GATCC
BbrPI 508 CACGTG CAC^GTG
BbsI 982 GTCTTC GAAGAC(2/6)
BglI 304 GCCCTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 982 GTCTTC GAAGAC(2/6)
BsaAI 508 CACGTG [CT]AC^GT[GA]
BsaHI 1414 GACGCC G[GA]^CG[CT]C
BsgI 795 CTGCAC GTGCAG(16/14)
BsmAI 658 GTCTC GTCTC(1/5)
BsmI 120 GAATGC GAATGC(1/-1)
BspHI 860 TCATGA T^CATGA
BspLU11I 1289 ACATGT A^CATGT
BsrBI 1315 GAGCGG CCGCTC(-3/-3)
BstAPI 846 GCACAACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 162 CCTGAGG CC^T[ACGT]AGG
DraIII 847 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1185 GAAGAG CTCTTC(1/4)
Eco81I 162 CCTGAGG CC^T[ACGT]AGG
Hin1I 1414 GACGCC G[GA]^CG[CT]C
HindIII 1191 AAGCTT A^AGCTT
Ksp632I 1185 GAAGAG CTCTTC(1/4)
KspI 5 CCGCGG CCGC^GG
MluI 47 ACGCGT A^CGCGT
MluNI 1004 TGGCCA TGG^CCA
MscI 1004 TGGCCA TGG^CCA
NcoI 440 CCATGG C^CATGG
PflMI 504 CCAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 508 CACGTG CAC^GTG
PmlI 508 CACGTG CAC^GTG
PpuMI 1171 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 63 AACGTT AA^CGTT
RcaI 860 TCATGA T^CATGA
SacI 1209 GAGCTC GAGCT^C
SacII 5 CCGCGG CCGC^GG
SfiI 303 GGCCCTGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 94 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1100 CCCGGG CCC^GGG
Van91I 504 CCAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1028 CCAAGCGTTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1100 CCCGGG C^CCGGG
XmaI 1100 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Alw44I 1297 GTGCAC G^TGCAC
1358 GTGCAC
ApaLI 1297 GTGCAC G^TGCAC
1358 GTGCAC
AvaI 299 CTCGGG C^[CT]CG[GA]G
1100 CCCGGG
AviII 722 TGCGCA TGC^GCA
1223 TGCGCA
BsoBI 299 CTCGGG C^[CT]CG[GA]G
1100 CCCGGG
BsrFI 95 GCCGGC [GA]^CCGG[CT]
947 GCCGGC
BssSI 224 CTCGTG CACGAG(-5/-1)
694 CTCGTG
BstYI 761 AGATCC [GA]^GATC[CT]
1104 GGATCC
Cfr10I 95 GCCGGC [GA]^CCGG[CT]
947 GCCGGC
FspI 722 TGCGCA TGC^GCA
1223 TGCGCA
HincII 811 GTCAAC GT[CT]^[GA]AC
1336 GTCAAC
HindII 811 GTCAAC GT[CT]^[GA]AC
1336 GTCAAC
HphI 964 GGTGA GGTGA(8/7)
1272 GGTGA
MflI 761 AGATCC [GA]^GATC[CT]
1104 GGATCC
NaeI 95 GCCGGC GCC^GGC
947 GCCGGC
NgoMIV 95 GCCGGC G^CCGGC
947 GCCGGC
TaqI 578 TCGA T^CGA
1202 TCGA
TthHB8I 578 TCGA T^CGA
1202 TCGA
XhoII 761 AGATCC [GA]^GATC[CT]
1104 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI Aor51HI ApaI AspHI
AvaII BanI BanII BbvI BcgI
BcnI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI Eco47III EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HpaII ItaI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TseI Tsp45I
TspRI