(Circular) (Six-base) MAPSORT of: pFN21AB3799.seq Check: 726 from: 1 to: 6160 pFN21AB3799 6160 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3970 278 Size: 53 83 186 936 2434 2468 Fragments arranged by size: 2468 2434 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3165 3503 1937 Size: 1228 338 4594 Fragments arranged by size: 4594 1228 338 Acc65I G'GTAC_C Cuts at: 3481 3481 Size: 6160 AclI AA'CG_TT Cuts at: 1563 2069 4287 4660 1563 Size: 506 2218 373 3063 Fragments arranged by size: 3063 2218 506 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3209 4223 5607 5875 1632 Size: 1577 1014 1384 268 1917 Fragments arranged by size: 1917 1577 1384 1014 268 AfeI AGC'GCT Cuts at: 2151 2198 2785 2151 Size: 47 587 5526 Fragments arranged by size: 5526 587 47 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5963 AflIII A'CryG_T Cuts at: 1203 2052 2457 3294 5059 6036 1203 Size: 849 405 837 1765 977 1327 Fragments arranged by size: 1765 1327 977 849 837 405 AgeI A'CCGG_T Cuts at: 4991 4991 Size: 6160 AhdI GACnn_n'nnGTC Cuts at: 4889 4889 Size: 6160 AleI CACnn'nnGTG Cuts at: 1166 2655 2834 1166 Size: 1489 179 4492 Fragments arranged by size: 4492 1489 179 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2129 2429 1184 Size: 945 300 4915 Fragments arranged by size: 4915 945 300 AlwNI CAG_nnn'CTG Cuts at: 2116 2167 2188 3903 5475 2116 Size: 51 21 1715 1572 2801 Fragments arranged by size: 2801 1715 1572 51 21 ApaI G_GGCC'C Cuts at: 2208 2311 2320 2208 Size: 103 9 6048 Fragments arranged by size: 6048 103 9 ApaLI G'TGCA_C Cuts at: 3302 3363 4217 5373 3302 Size: 61 854 1156 4089 Fragments arranged by size: 4089 1156 854 61 ApoI r'AATT_y Cuts at: 1493 3469 3695 3847 5956 1493 Size: 1976 226 152 2109 1697 Fragments arranged by size: 2109 1976 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4714 160 Size: 872 3682 1606 Fragments arranged by size: 3682 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6160 AvaI C'yCGr_G Cuts at: 1870 1943 2304 3105 3485 1870 Size: 73 361 801 380 4545 Fragments arranged by size: 4545 801 380 361 73 BamHI G'GATC_C Cuts at: 3109 3490 3857 3109 Size: 381 367 5412 Fragments arranged by size: 5412 381 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2139 2325 2413 2611 3481 Size: 325 217 627 352 186 88 198 870 Cuts at: 3481 4936 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 870 627 352 325 217 198 186 88 BanII G_rGCy'C Cuts at: 1391 2208 2311 2320 2903 3062 3218 3479 1391 Size: 817 103 9 583 159 156 261 4072 Fragments arranged by size: 4072 817 583 261 159 156 103 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2980 928 Size: 971 1081 4108 Fragments arranged by size: 4108 1081 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2230 2264 2459 2493 3418 3452 4351 Size: 34 293 34 195 34 925 34 899 Cuts at: 4351 4385 1903 Size: 34 3678 Fragments arranged by size: 3678 925 899 293 195 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4052 5262 4052 Size: 1210 4950 BclI T'GATC_A Cuts at: 1548 1548 Size: 6160 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2315 4771 136 Size: 107 122 71 631 605 643 2456 1525 Fragments arranged by size: 2456 1525 643 631 605 122 107 71 BglII A'GATC_T Cuts at: 6155 6155 Size: 6160 BlpI GC'TnA_GC Cuts at: 3577 3577 Size: 6160 Bme1580I G_kGCm'C Cuts at: 1790 2142 2208 2311 2320 2328 2418 3306 3367 Size: 352 66 103 9 8 90 888 61 Cuts at: 3367 4221 5377 1790 Size: 854 1156 2573 Fragments arranged by size: 2573 1156 888 854 352 103 90 66 61 9 8 BmgBI CAC'GTC Cuts at: 1318 2421 2460 1318 Size: 1103 39 5018 Fragments arranged by size: 5018 1103 39 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3891 4849 442 Size: 416 53 83 399 24 2474 958 1753 Fragments arranged by size: 2474 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6160 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3232 3232 Size: 6160 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2887 3397 3463 4820 1239 Size: 1648 510 66 1357 2579 Fragments arranged by size: 2579 1648 1357 510 66 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4285 5150 5448 4285 Size: 865 298 4997 Fragments arranged by size: 4997 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4823 882 Size: 617 3324 2219 Fragments arranged by size: 3324 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2515 3951 493 Size: 711 1311 1436 2702 Fragments arranged by size: 2702 1436 1311 711 BsaBI GATnn'nnATC Cuts at: 6154 6154 Size: 6160 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3420 3967 4349 4984 Size: 53 83 186 936 1887 547 382 635 Cuts at: 4984 275 Size: 1451 Fragments arranged by size: 1887 1451 936 635 547 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4593 4991 5265 5412 5823 4593 Size: 398 274 147 411 4930 Fragments arranged by size: 4930 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5935 Fragments arranged by size: 5935 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6160 BseYI C'CCAG_C Cuts at: 2135 2146 2188 5363 5859 2135 Size: 11 42 3175 496 2436 Fragments arranged by size: 3175 2436 496 42 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2785 1163 Size: 573 1049 4538 Fragments arranged by size: 4538 1049 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3610 4371 5399 1540 Size: 275 186 1609 761 1028 2301 Fragments arranged by size: 2301 1609 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2230 3218 3306 3367 4221 4306 5377 2230 Size: 988 88 61 854 85 1071 3013 Fragments arranged by size: 3013 1071 988 854 88 85 61 BsmI GAATG_Cn' Cuts at: 2131 3671 3764 2131 Size: 1540 93 4527 Fragments arranged by size: 4527 1540 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2142 2208 2230 2311 2320 2328 2418 Size: 399 352 66 22 81 9 8 90 Cuts at: 2418 2903 3062 3218 3306 3367 3479 4221 4306 Size: 485 159 156 88 61 112 742 85 Cuts at: 4306 5377 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 485 399 352 159 156 112 90 88 85 81 66 61 22 9 8 BspEI T'CCGG_A Cuts at: 5823 5823 Size: 6160 BspHI T'CATG_A Cuts at: 2865 4049 5779 2865 Size: 1184 1730 3246 Fragments arranged by size: 3246 1730 1184 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3515 844 Size: 2671 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3322 4047 1394 Size: 429 1499 725 3507 Fragments arranged by size: 3507 1499 725 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4655 4829 65 Size: 1183 3407 174 1396 Fragments arranged by size: 3407 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2100 2952 4804 4991 1700 Size: 110 290 852 1852 187 2869 Fragments arranged by size: 2869 1852 852 290 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6160 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6160 BssSI C'ACGA_G Cuts at: 2229 2699 4220 5232 2229 Size: 470 1521 1012 3157 Fragments arranged by size: 3157 1521 1012 470 BstAPI GCAn_nnn'nTGC Cuts at: 794 2857 794 Size: 2063 4097 BstBI TT'CG_AA Cuts at: 4964 4964 Size: 6160 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6160 BstXI CCAn_nnnn'nTGG Cuts at: 2657 2713 3040 3430 2657 Size: 56 327 390 5387 Fragments arranged by size: 5387 390 327 56 BstYI r'GATC_y Cuts at: 1972 2766 3109 3490 3857 4242 4259 5711 5797 Size: 794 343 381 367 385 17 1452 86 Cuts at: 5797 5809 6155 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 794 385 381 367 346 343 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2168 2168 Size: 6160 BtgI C'CryG_G Cuts at: 513 1065 2004 2010 2320 2445 2656 2977 513 Size: 552 939 6 310 125 211 321 3696 Fragments arranged by size: 3696 939 552 321 310 211 125 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5421 Fragments arranged by size: 5421 683 56 BtsI GCAGTG_nn' Cuts at: 2040 2230 2989 3116 3292 3676 4470 4490 2040 Size: 190 759 127 176 384 794 20 3710 Fragments arranged by size: 3710 794 759 384 190 176 127 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6160 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6125 DraI TTT'AAA Cuts at: 3464 3464 Size: 6160 DraIII CAC_nnn'GTG Cuts at: 2857 2857 Size: 6160 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5167 809 Size: 507 3851 1802 Fragments arranged by size: 3851 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2448 2950 Size: 54 1425 50 275 18 177 441 502 Cuts at: 2950 3009 3327 3607 4496 6125 8 Size: 59 318 280 889 1629 43 Fragments arranged by size: 1629 1425 889 502 441 318 280 275 177 59 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3607 1537 Size: 275 1795 4090 Fragments arranged by size: 4090 1795 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3185 4090 1353 Size: 1832 905 3423 Fragments arranged by size: 3423 1832 905 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2087 3248 4747 5121 5267 387 Size: 1064 138 498 1161 1499 374 146 1280 Fragments arranged by size: 1499 1280 1161 1064 498 374 146 138 EcoICRI GAG'CTC Cuts at: 3216 3216 Size: 6160 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2887 3209 3397 3463 4223 4820 5607 Size: 393 1255 322 188 66 760 597 787 Cuts at: 5607 5875 1239 Size: 268 1524 Fragments arranged by size: 1524 1255 787 760 597 393 322 268 188 66 EcoO109I rG'GnC_Cy Cuts at: 2205 2308 2316 2397 3177 3267 2205 Size: 103 8 81 780 90 5098 Fragments arranged by size: 5098 780 103 90 81 8 EcoRI G'AATT_C Cuts at: 3469 3847 3469 Size: 378 5782 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3214 3214 Size: 6160 FspI TGC'GCA Cuts at: 2729 3230 4666 2729 Size: 501 1436 4223 Fragments arranged by size: 4223 1436 501 FspAI rTGC'GCAy Cuts at: 2729 2729 Size: 6160 HaeII r_GCGC'y Cuts at: 1678 2153 2200 2787 5307 1678 Size: 475 47 587 2520 2531 Fragments arranged by size: 2531 2520 587 475 47 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3205 3237 4801 4833 4875 Size: 32 135 32 1633 32 1564 32 42 Cuts at: 4875 4907 1373 Size: 32 2626 Fragments arranged by size: 2626 1633 1564 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2818 3343 3504 3750 669 Size: 988 281 880 525 161 246 3079 Fragments arranged by size: 3079 988 880 525 281 246 161 HindIII A'AGCT_T Cuts at: 748 3196 748 Size: 2448 3712 HpaI GTT'AAC Cuts at: 3750 3750 Size: 6160 KpnI G_GTAC'C Cuts at: 3485 3485 Size: 6160 MfeI C'AATT_G Cuts at: 3759 3759 Size: 6160 MluI A'CGCG_T Cuts at: 2052 6036 2052 Size: 3984 2176 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2691 5274 5458 1305 Size: 1386 2583 184 2007 Fragments arranged by size: 2583 2007 1386 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3011 10 Size: 54 1425 343 1179 3159 Fragments arranged by size: 3159 1425 1179 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2517 2655 2699 2834 3299 4118 4477 Size: 648 1351 138 44 135 465 819 359 Cuts at: 4477 4636 518 Size: 159 2042 Fragments arranged by size: 2042 1351 819 648 465 359 159 138 135 44 MspA1I CmG'CkG Cuts at: 1606 2012 2113 2185 2359 2608 3187 3426 3576 Size: 406 101 72 174 249 579 239 150 Cuts at: 3576 4253 5401 5646 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 579 406 249 245 239 174 150 101 72 NaeI GCC'GGC Cuts at: 2102 2954 2102 Size: 852 5308 NcoI C'CATG_G Cuts at: 513 1065 2004 2445 513 Size: 552 939 441 4228 Fragments arranged by size: 4228 939 552 441 NdeI CA'TA_TG Cuts at: 387 387 Size: 6160 NgoMIV G'CCGG_C Cuts at: 2100 2952 2100 Size: 852 5308 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6160 NotI GC'GGCC_GC Cuts at: 3607 3607 Size: 6160 NspI r_CATG'y Cuts at: 1132 1229 2494 2864 3298 3518 5063 1132 Size: 97 1265 370 434 220 1545 2229 Fragments arranged by size: 2229 1545 1265 434 370 220 97 PasI CC'CwG_GG Cuts at: 1348 2311 1348 Size: 963 5197 PciI A'CATG_T Cuts at: 3294 5059 3294 Size: 1765 4395 PflMI CCAn_nnn'nTGG Cuts at: 1108 2515 1108 Size: 1407 4753 PmeI GTTT'AAAC Cuts at: 3464 3464 Size: 6160 PmlI CAC'GTG Cuts at: 2515 2515 Size: 6160 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4229 4261 5742 5774 4229 Size: 32 1481 32 4615 Fragments arranged by size: 4615 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3177 3177 Size: 6160 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6160 PsiI TTA'TAA Cuts at: 3730 3730 Size: 6160 PspOMI G'GGCC_C Cuts at: 2204 2307 2316 2204 Size: 103 9 6048 Fragments arranged by size: 6048 103 9 PstI C_TGCA'G Cuts at: 830 2364 3186 3354 3512 830 Size: 1534 822 168 158 3478 Fragments arranged by size: 3478 1534 822 168 158 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6160 PvuII CAG'CTG Cuts at: 2113 2185 2359 2608 3187 2113 Size: 72 174 249 579 5086 Fragments arranged by size: 5086 579 249 174 72 SacI G_AGCT'C Cuts at: 3218 3218 Size: 6160 SacII CC_GC'GG Cuts at: 2013 2013 Size: 6160 SalI G'TCGA_C Cuts at: 1936 3502 1936 Size: 1566 4594 SanDI GG'GwC_CC Cuts at: 3177 3177 Size: 6160 SbfI CC_TGCA'GG Cuts at: 3512 3512 Size: 6160 ScaI AGT'ACT Cuts at: 4408 6120 4408 Size: 1712 4448 SfcI C'TryA_G Cuts at: 826 1045 2236 2360 3154 3182 3350 3508 4643 Size: 219 1191 124 794 28 168 158 1135 Cuts at: 4643 5324 5515 826 Size: 681 191 1471 Fragments arranged by size: 1471 1191 1135 794 681 219 191 168 158 124 28 SfiI GGCCn_nnn'nGGCC Cuts at: 2315 2315 Size: 6160 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6160 SgrAI Cr'CCGG_yG Cuts at: 1700 2100 1700 Size: 400 5760 SmaI CCC'GGG Cuts at: 1872 3107 3487 1872 Size: 1235 380 4545 Fragments arranged by size: 4545 1235 380 SmlI C'TyrA_G Cuts at: 820 1017 1943 4264 5165 5427 820 Size: 197 926 2321 901 262 1553 Fragments arranged by size: 2321 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3951 493 Size: 3458 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6160 SphI G_CATG'C Cuts at: 1132 3518 1132 Size: 2386 3774 SspI AAT'ATT Cuts at: 5 52 4084 5 Size: 47 4032 2081 Fragments arranged by size: 4032 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6160 StyI C'CwwG_G Cuts at: 513 1065 2004 2130 2445 2531 3330 3599 513 Size: 552 939 126 315 86 799 269 3074 Fragments arranged by size: 3074 939 799 552 315 269 126 86 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2571 2638 4187 4204 4357 4542 4987 Size: 1255 991 67 1549 17 153 185 445 Cuts at: 4987 325 Size: 1498 Fragments arranged by size: 1549 1498 1255 991 445 185 153 67 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4406 6118 96 Size: 274 80 33 51 1443 2429 1712 138 Fragments arranged by size: 2429 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6120 6152 6120 Size: 32 6128 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6160 XbaI T'CTAG_A Cuts at: 3496 3496 Size: 6160 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3040 3040 Size: 6160 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6160 XmaI C'CCGG_G Cuts at: 1870 3105 3485 1870 Size: 1235 380 4545 Fragments arranged by size: 4545 1235 380 XmnI GAAnn'nnTTC Cuts at: 4289 4289 Size: 6160 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3968 276 Size: 53 83 186 936 2434 2468 Fragments arranged by size: 2468 2434 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoRV FseI KasI NarI NruI NsiI PacI PfoI PspXI PsrI RsrII SapI SexAI SfoI SrfI SwaI