Restriction map for
pFN21AE3225
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccI AccIII AcsI
AflIII AgeI Alw44I AocI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BalI BamHI
BbvCI BcgI BciVI BclI BlnI
BsaBI BsaWI BseAI BsiWI BsmBI
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I Bsu36I Cfr10I ClaI
DraI DraIII DrdI Eco81I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI MamI MfeI
MluI MluNI MroI MscI MslI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
PvuI PvuII SalI SapI SexAI
SfuI SgrAI SnaBI SphI SspI
SwaI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AclI 882 AACGTT AA^CGTT
AcyI 1213 GGCGCC G[GA]^CG[CT]C
AflII 438 CTTAAG C^TTAAG
AhdI 1352 GACACTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 315 GGATC GGATC(4/5)
Aor51HI 53 AGCGCT AGC^GCT
ApaI 1184 GGGCCC GGGCC^C
AspEI 1352 GACACTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1435 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 1213 GGCGCC G^G[CT][GA]CC
BbeI 1213 GGCGCC GGCGC^C
BbrPI 1024 CACGTG CAC^GTG
BbsI 677 GAAGAC GAAGAC(2/6)
BfrI 438 CTTAAG C^TTAAG
BglII 458 AGATCT A^GATCT
BpuAI 677 GAAGAC GAAGAC(2/6)
BsaAI 1024 CACGTG [CT]AC^GT[GA]
BsaHI 1213 GGCGCC G[GA]^CG[CT]C
BseRI 1290 CTCCTC GAGGAG(10/8)
BsgI 1027 GTGCAG GTGCAG(16/14)
BsmI 567 GCATTC GAATGC(1/-1)
BstXI 1068 CCACCTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 458 AGATCT [GA]^GATC[CT]
CpoI 64 CGGACCG CG^G[AT]CCG
Eam1105I 1352 GACACTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 53 AGCGCT AGC^GCT
EcoNI 1455 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 837 GCGGG CCCGC(4/6)
HgaI 1502 GCGTC GACGC(5/10)
Hin1I 1213 GGCGCC G[GA]^CG[CT]C
HincII 1091 GTCAAC GT[CT]^[GA]AC
HindII 1091 GTCAAC GT[CT]^[GA]AC
KasI 1213 GGCGCC G^GCGCC
KspI 32 CCGCGG CCGC^GG
MflI 458 AGATCT [GA]^GATC[CT]
NarI 1213 GGCGCC GG^CGCC
NcoI 1332 CCATGG C^CATGG
NspI 1340 ACATGC [GA]CATG^[CT]
PflFI 1435 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 644 CCAATCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1024 CACGTG CAC^GTG
PmlI 1024 CACGTG CAC^GTG
Psp1406I 882 AACGTT AA^CGTT
RsrII 64 CGGACCG CG^G[AT]CCG
SacI 6 GAGCTC GAGCT^C
SacII 32 CCGCGG CCGC^GG
SbfI 1455 CCTGCAGG CCTGCA^GG
ScaI 305 AGTACT AGT^ACT
SfiI 36 GGCCGACAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1213 GGCGCC GGC^GCC
SmaI 11 CCCGGG CCC^GGG
SmlI 438 CTTAAG C^T[CT][GA]AG
SpeI 685 ACTAGT A^CTAGT
Sse8387I 1455 CCTGCAGG CCTGCA^GG
StuI 252 AGGCCT AGG^CCT
TaqI 729 TCGA T^CGA
Tth111I 1435 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 729 TCGA T^CGA
Van91I 644 CCAATCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 766 CCAGCCGCCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 458 AGATCT [GA]^GATC[CT]
XmaCI 11 CCCGGG C^CCGGG
XmaI 11 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
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AccII 33 CGCG CG^CG
1501 CGCG
AlwNI 976 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1414 CAGTGGCTG
AspHI 6 GAGCTC G[AT]GC[AT]^C
1021 GAGCAC
AvaI 11 CCCGGG C^[CT]CG[GA]G
542 CTCGGG
BglI 16 GCCGAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
37 GCCGACAGGGC
BlpI 3 GCTGAGC GC^T[ACGT]AGC
981 GCTGAGC
Bpu1102I 3 GCTGAGC GC^T[ACGT]AGC
981 GCTGAGC
BsaI 530 GAGACC GGTCTC(1/5)
1425 GGTCTC
BsiEI 35 CGGCCG CG[GA][CT]^CG
74 CGGCCG
BsiHKAI 6 GAGCTC G[AT]GC[AT]^C
1021 GAGCAC
BsmFI 161 GTCCC GGGAC(10/14)
1374 GTCCC
BsoBI 11 CCCGGG C^[CT]CG[GA]G
542 CTCGGG
Bsp1407I 446 TGTACA T^GTACA
859 TGTACA
BspHI 92 TCATGA T^CATGA
233 TCATGA
BsrGI 446 TGTACA T^GTACA
859 TGTACA
BstEII 626 GGTCACC G^GT[ACGT]ACC
781 GGTCACC
BstPI 626 GGTCACC G^GT[ACGT]ACC
781 GGTCACC
BstUI 33 CGCG CG^CG
1501 CGCG
CelII 3 GCTGAGC GC^T[ACGT]AGC
981 GCTGAGC
DpnII 316 GATC ^GATC
459 GATC
EaeI 35 CGGCCG [CT]^GGCC[GA]
74 CGGCCG
EagI 35 CGGCCG C^GGCCG
74 CGGCCG
EarI 1303 CTCTTC CTCTTC(1/4)
289 GAAGAG
EclXI 35 CGGCCG C^GGCCG
74 CGGCCG
Eco52I 35 CGGCCG C^GGCCG
74 CGGCCG
EcoO65I 626 GGTCACC G^GT[ACGT]ACC
781 GGTCACC
Ksp632I 1303 CTCTTC CTCTTC(1/4)
289 GAAGAG
MaeII 883 ACGT A^CGT
1025 ACGT
MboI 316 GATC ^GATC
459 GATC
MspA1I 32 CCGCGG C[AC]G^C[GT]G
976 CAGCGG
MvnI 33 CGCG CG^CG
1501 CGCG
NdeII 316 GATC ^GATC
459 GATC
PleI 680 GACTC GAGTC(4/5)
720 GAGTC
PpuMI 159 GGGTCCC [GA]G^G[AT]CC[CT]
1463 AGGTCCT
PstI 1138 CTGCAG CTGCA^G
1456 CTGCAG
RcaI 92 TCATGA T^CATGA
233 TCATGA
Sau3AI 316 GATC ^GATC
459 GATC
SspBI 446 TGTACA T^GTACA
859 TGTACA
TfiI 829 GAATC G^A[AT]TC
1173 GAATC
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AvaII BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BsiYI BslI BsmAI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DraII
DsaI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboII MnlI MseI
MspI MvaI MwoI NciI NlaIII
NlaIV RsaI Sau96I ScrFI SfaNI
SfcI StyI Tru9I TseI Tsp45I
Tsp509I TspRI