(Circular) (Six-base) MAPSORT of: pFN21AE3225.seq Check: 5764 from: 1 to: 6256 pFN21AE3225 6256 bp With 182 enzymes: SgfI * March 28, 2012 14:21 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2240 2240 Size: 6256 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4066 278 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3599 1937 Size: 1662 4594 Acc65I G'GTAC_C Cuts at: 3577 3577 Size: 6256 AclI AA'CG_TT Cuts at: 1563 2888 4383 4756 1563 Size: 1325 1495 373 3063 Fragments arranged by size: 3063 1495 1325 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3035 3570 4319 5703 5971 1632 Size: 1403 535 749 1384 268 1917 Fragments arranged by size: 1917 1403 1384 749 535 268 AfeI AGC'GCT Cuts at: 2060 2060 Size: 6256 AflII C'TTAA_G Cuts at: 820 1017 2443 820 Size: 197 1426 4633 Fragments arranged by size: 4633 1426 197 AflIII A'CryG_T Cuts at: 1203 5155 6132 1203 Size: 3952 977 1327 Fragments arranged by size: 3952 1327 977 AgeI A'CCGG_T Cuts at: 5087 5087 Size: 6256 AhdI GACnn_n'nnGTC Cuts at: 3362 4985 3362 Size: 1623 4633 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6256 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6256 AlwNI CAG_nnn'CTG Cuts at: 2986 3424 3999 5571 2986 Size: 438 575 1572 3671 Fragments arranged by size: 3671 1572 575 438 ApaI G_GGCC'C Cuts at: 3193 3193 Size: 6256 ApaLI G'TGCA_C Cuts at: 4313 5469 4313 Size: 1156 5100 ApoI r'AATT_y Cuts at: 1493 3565 3791 3943 6052 1493 Size: 2072 226 152 2109 1697 Fragments arranged by size: 2109 2072 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4810 160 Size: 872 3778 1606 Fragments arranged by size: 3778 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 AvaI C'yCGr_G Cuts at: 1870 1943 2016 2547 3581 1870 Size: 73 73 531 1034 4545 Fragments arranged by size: 4545 1034 531 73 73 BamHI G'GATC_C Cuts at: 3586 3953 3586 Size: 367 5889 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3218 3577 5032 618 Size: 325 217 627 1431 359 1455 1842 Fragments arranged by size: 1842 1455 1431 627 359 325 217 BanII G_rGCy'C Cuts at: 1391 2015 2334 3163 3193 3575 1391 Size: 624 319 829 30 382 4072 Fragments arranged by size: 4072 829 624 382 319 30 BbeI G_GCGC'C Cuts at: 3222 3222 Size: 6256 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2689 928 Size: 971 790 4495 Fragments arranged by size: 4495 971 790 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4447 4481 1903 Size: 34 50 34 2426 34 3678 Fragments arranged by size: 3678 2426 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4148 5358 4148 Size: 1210 5046 BclI T'GATC_A Cuts at: 1548 1548 Size: 6256 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2027 2048 4867 Size: 107 122 71 631 605 355 21 2819 Cuts at: 4867 136 Size: 1525 Fragments arranged by size: 2819 1525 631 605 355 122 107 71 21 BglII A'GATC_T Cuts at: 2463 6251 2463 Size: 3788 2468 BlpI GC'TnA_GC Cuts at: 2009 2987 3673 2009 Size: 978 686 4592 Fragments arranged by size: 4592 978 686 Bme1580I G_kGCm'C Cuts at: 1790 3193 4317 5473 1790 Size: 1403 1124 1156 2573 Fragments arranged by size: 2573 1403 1156 1124 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6256 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2116 2302 3269 Size: 416 53 83 399 24 699 186 967 Cuts at: 3269 3987 4945 442 Size: 718 958 1753 Fragments arranged by size: 1753 967 958 718 699 416 399 186 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6256 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2320 2353 2320 Size: 33 6223 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2299 2600 3158 3463 4916 1239 Size: 1060 301 558 305 1453 2579 Fragments arranged by size: 2579 1453 1060 558 305 301 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4381 5246 5544 4381 Size: 865 298 5093 Fragments arranged by size: 5093 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2529 3436 4919 882 Size: 617 1030 907 1483 2219 Fragments arranged by size: 2219 1483 1030 907 617 BsaAI yAC'GTr Cuts at: 493 1204 3031 4047 493 Size: 711 1827 1016 2702 Fragments arranged by size: 2702 1827 1016 711 BsaBI GATnn'nnATC Cuts at: 6250 6250 Size: 6256 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3219 4063 4445 5080 Size: 53 83 186 936 1686 844 382 635 Cuts at: 5080 275 Size: 1451 Fragments arranged by size: 1686 1451 936 844 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4689 5087 5361 5508 5919 4689 Size: 398 274 147 411 5026 Fragments arranged by size: 5026 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2573 2603 3278 3281 3308 Size: 30 165 30 1170 30 675 3 27 Cuts at: 3308 3311 3458 3488 3530 3560 1178 Size: 3 147 30 42 30 3874 Fragments arranged by size: 3874 1170 675 165 147 42 30 30 30 30 30 27 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3286 1186 Size: 2100 4156 BseYI C'CCAG_C Cuts at: 5459 5955 5459 Size: 496 5760 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3053 1163 Size: 573 1317 4366 Fragments arranged by size: 4366 1317 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2043 2082 3706 4467 5495 1540 Size: 275 186 42 39 1624 761 1028 2301 Fragments arranged by size: 2301 1624 1028 761 275 186 42 39 BsiHKAI G_wGCw'C Cuts at: 2015 3030 4317 4402 5473 2015 Size: 1015 1287 85 1071 2798 Fragments arranged by size: 2798 1287 1071 1015 85 BsmI GAATG_Cn' Cuts at: 2572 3767 3860 2572 Size: 1195 93 4968 Fragments arranged by size: 4968 1195 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2334 3030 3163 3193 3575 4317 Size: 399 225 319 696 133 30 382 742 Cuts at: 4317 4402 5473 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 696 399 382 319 225 133 85 30 BspEI T'CCGG_A Cuts at: 5919 5919 Size: 6256 BspHI T'CATG_A Cuts at: 2097 2238 4145 5875 2097 Size: 141 1907 1730 2478 Fragments arranged by size: 2478 1907 1730 141 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2240 2624 3498 3611 844 Size: 1396 384 874 113 3489 Fragments arranged by size: 3489 1396 874 384 113 BsrBI CCG'CTC Cuts at: 1394 1823 4143 1394 Size: 429 2320 3507 Fragments arranged by size: 3507 2320 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4751 4925 65 Size: 1183 3503 174 1396 Fragments arranged by size: 3503 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4900 5087 1700 Size: 110 3090 187 2869 Fragments arranged by size: 3090 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2451 2864 96 Size: 2355 413 3488 Fragments arranged by size: 3488 2355 413 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6256 BssSI C'ACGA_G Cuts at: 4316 5328 4316 Size: 1012 5244 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6256 BstBI TT'CG_AA Cuts at: 5060 5060 Size: 6256 BstEII G'GTnAC_C Cuts at: 1184 2631 2786 1184 Size: 1447 155 4654 Fragments arranged by size: 4654 1447 155 BstXI CCAn_nnnn'nTGG Cuts at: 3080 3080 Size: 6256 BstYI r'GATC_y Cuts at: 1972 2463 3586 3953 4338 4355 5807 5893 5905 Size: 491 1123 367 385 17 1452 86 12 Cuts at: 5905 6251 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1123 491 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2037 3001 3337 513 Size: 552 972 964 336 3432 Fragments arranged by size: 3432 972 964 552 336 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2045 487 Size: 683 56 819 4698 Fragments arranged by size: 4698 819 683 56 BtsI GCAGTG_nn' Cuts at: 2806 3772 4566 4586 2806 Size: 966 794 20 4476 Fragments arranged by size: 4476 966 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6256 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6221 DraI TTT'AAA Cuts at: 3560 3560 Size: 6256 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5263 809 Size: 507 3947 1802 Fragments arranged by size: 3947 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2079 3703 Size: 54 1425 50 275 18 210 39 1624 Cuts at: 3703 4592 6221 8 Size: 889 1629 43 Fragments arranged by size: 1629 1624 1425 889 275 210 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 2040 2079 3703 1537 Size: 275 228 39 1624 4090 Fragments arranged by size: 4090 1624 275 228 39 EarI CTCTTCn'nnn_ Cuts at: 1353 2289 3314 4186 1353 Size: 936 1025 872 3423 Fragments arranged by size: 3423 1025 936 872 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4843 5217 5363 387 Size: 1064 138 3254 374 146 1280 Fragments arranged by size: 3254 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2013 2013 Size: 6256 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2299 2600 3035 3158 3463 3570 4319 Size: 393 667 301 435 123 305 107 749 Cuts at: 4319 4916 5703 5971 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 749 667 597 435 393 305 301 268 123 107 EcoNI CCTnn'n_nnAGG Cuts at: 3464 3464 Size: 6256 EcoO109I rG'GnC_Cy Cuts at: 2165 3128 3189 3190 3469 2165 Size: 963 61 1 279 4952 Fragments arranged by size: 4952 963 279 61 1 EcoRI G'AATT_C Cuts at: 3565 3943 3565 Size: 378 5878 FspI TGC'GCA Cuts at: 4762 4762 Size: 6256 HaeII r_GCGC'y Cuts at: 1678 2062 3222 3257 3486 5403 1678 Size: 384 1160 35 229 1917 2531 Fragments arranged by size: 2531 1917 1160 384 229 35 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2079 2111 2448 2480 2573 Size: 32 135 32 507 32 337 32 93 Cuts at: 2573 2605 3278 3310 3335 3348 3367 3380 3458 Size: 32 673 32 25 13 19 13 78 Cuts at: 3458 3490 3530 3562 4897 4929 4971 5003 1373 Size: 32 40 32 1335 32 42 32 2626 Fragments arranged by size: 2626 1335 673 507 337 135 93 78 42 40 32 32 32 32 32 32 32 32 32 32 25 19 13 13 HincII GTy'rAC Cuts at: 669 1657 1938 3098 3600 3846 669 Size: 988 281 1160 502 246 3079 Fragments arranged by size: 3079 1160 988 502 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6256 HpaI GTT'AAC Cuts at: 3846 3846 Size: 6256 KasI G'GCGC_C Cuts at: 3218 3218 Size: 6256 KpnI G_GTAC'C Cuts at: 3581 3581 Size: 6256 MfeI C'AATT_G Cuts at: 3855 3855 Size: 6256 MluI A'CGCG_T Cuts at: 6132 6132 Size: 6256 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2415 5370 5554 1305 Size: 1110 2955 184 2007 Fragments arranged by size: 2955 2007 1110 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4434 Fragments arranged by size: 4434 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4214 4573 4732 518 Size: 648 3048 359 159 2042 Fragments arranged by size: 3048 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2039 2983 3672 4349 5497 5742 1606 Size: 433 944 689 677 1148 245 2120 Fragments arranged by size: 2120 1148 944 689 677 433 245 NarI GG'CG_CC Cuts at: 3219 3219 Size: 6256 NcoI C'CATG_G Cuts at: 513 1065 3337 513 Size: 552 2272 3432 Fragments arranged by size: 3432 2272 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6256 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6256 NotI GC'GGCC_GC Cuts at: 3703 3703 Size: 6256 NspI r_CATG'y Cuts at: 1132 1229 3349 3614 5159 1132 Size: 97 2120 265 1545 2229 Fragments arranged by size: 2229 2120 1545 265 97 PasI CC'CwG_GG Cuts at: 1348 3125 1348 Size: 1777 4479 PciI A'CATG_T Cuts at: 5155 5155 Size: 6256 PflMI CCAn_nnn'nTGG Cuts at: 1108 2655 1108 Size: 1547 4709 PfoI T'CCnGG_A Cuts at: 3515 3515 Size: 6256 PmeI GTTT'AAAC Cuts at: 3560 3560 Size: 6256 PmlI CAC'GTG Cuts at: 3031 3031 Size: 6256 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3389 3421 4325 4357 5838 5870 3389 Size: 32 904 32 1481 32 3775 Fragments arranged by size: 3775 1481 904 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2165 3469 2165 Size: 1304 4952 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6256 PsiI TTA'TAA Cuts at: 3826 3826 Size: 6256 PspOMI G'GGCC_C Cuts at: 3189 3189 Size: 6256 PstI C_TGCA'G Cuts at: 830 3147 3465 3608 830 Size: 2317 318 143 3478 Fragments arranged by size: 3478 2317 318 143 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6256 RsrII CG'GwC_CG Cuts at: 2070 2070 Size: 6256 SacI G_AGCT'C Cuts at: 2015 2015 Size: 6256 SacII CC_GC'GG Cuts at: 2040 2040 Size: 6256 SalI G'TCGA_C Cuts at: 1936 3598 1936 Size: 1662 4594 SanDI GG'GwC_CC Cuts at: 2165 2165 Size: 6256 SbfI CC_TGCA'GG Cuts at: 3465 3608 3465 Size: 143 6113 ScaI AGT'ACT Cuts at: 2312 4504 6216 2312 Size: 2192 1712 2352 Fragments arranged by size: 2352 2192 1712 SfcI C'TryA_G Cuts at: 826 1045 2978 3143 3289 3461 3604 4739 5420 Size: 219 1933 165 146 172 143 1135 681 Cuts at: 5420 5611 826 Size: 191 1471 Fragments arranged by size: 1933 1471 1135 681 219 191 172 165 146 143 SfiI GGCCn_nnn'nGGCC Cuts at: 2048 2048 Size: 6256 SfoI GGC'GCC Cuts at: 3220 3220 Size: 6256 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6256 SmaI CCC'GGG Cuts at: 1872 2018 3583 1872 Size: 146 1565 4545 Fragments arranged by size: 4545 1565 146 SmlI C'TyrA_G Cuts at: 820 1017 1943 2443 4360 5261 5523 820 Size: 197 926 500 1917 901 262 1553 Fragments arranged by size: 1917 1553 926 901 500 262 197 SnaBI TAC'GTA Cuts at: 493 4047 493 Size: 3554 2702 SpeI A'CTAG_T Cuts at: 152 2690 152 Size: 2538 3718 SphI G_CATG'C Cuts at: 1132 3614 1132 Size: 2482 3774 SspI AAT'ATT Cuts at: 5 52 4180 5 Size: 47 4128 2081 Fragments arranged by size: 4128 2081 47 StuI AGG'CCT Cuts at: 1517 2259 1517 Size: 742 5514 StyI C'CwwG_G Cuts at: 513 1065 2880 3079 3337 3695 513 Size: 552 1815 199 258 358 3074 Fragments arranged by size: 3074 1815 552 358 258 199 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2805 4283 4300 4453 4638 5083 325 Size: 1255 1225 1478 17 153 185 445 1498 Fragments arranged by size: 1498 1478 1255 1225 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2310 2451 2864 Size: 274 80 33 51 1443 333 141 413 Cuts at: 2864 4502 6214 96 Size: 1638 1712 138 Fragments arranged by size: 1712 1638 1443 413 333 274 141 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2630 2662 2837 2869 6216 6248 2630 Size: 32 175 32 3347 32 2638 Fragments arranged by size: 3347 2638 175 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3443 1316 Size: 2127 4129 XbaI T'CTAG_A Cuts at: 3592 3592 Size: 6256 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2778 2778 Size: 6256 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6256 XmaI C'CCGG_G Cuts at: 1870 2016 3581 1870 Size: 146 1565 4545 Fragments arranged by size: 4545 1565 146 XmnI GAAnn'nnTTC Cuts at: 4385 4385 Size: 6256 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4064 276 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI PvuII SapI SexAI SrfI SwaI