(Linear) (Six-base) MAPSORT of: KIEE3225.seq Check: 8998 from: 1 to: 1551 KIEE3225.orf With 182 enzymes: SgfI * March 22, 2012 16:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 235 1551 Size: 235 1316 AclI AA'CG_TT Cuts at: 0 883 1551 Size: 883 668 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1030 1551 Size: 1030 521 AfeI AGC'GCT Cuts at: 0 55 1551 Size: 55 1496 AflII C'TTAA_G Cuts at: 0 438 1551 Size: 438 1113 AhdI GACnn_n'nnGTC Cuts at: 0 1357 1551 Size: 1357 194 AlwNI CAG_nnn'CTG Cuts at: 0 981 1419 1551 Size: 981 438 132 Fragments arranged by size: 981 438 132 ApaI G_GGCC'C Cuts at: 0 1188 1551 Size: 1188 363 AvaI C'yCGr_G Cuts at: 0 11 542 1551 Size: 11 531 1009 Fragments arranged by size: 1009 531 11 BanI G'GyrC_C Cuts at: 0 1213 1551 Size: 1213 338 BanII G_rGCy'C Cuts at: 0 10 329 1158 1188 1551 Size: 10 319 829 30 363 Fragments arranged by size: 829 363 319 30 10 BbeI G_GCGC'C Cuts at: 0 1217 1551 Size: 1217 334 BbsI GAAGACnn'nnnn_ Cuts at: 0 684 1551 Size: 684 867 BglI GCCn_nnn'nGGC Cuts at: 0 22 43 1551 Size: 22 21 1508 Fragments arranged by size: 1508 22 21 BglII A'GATC_T Cuts at: 0 458 1551 Size: 458 1093 BlpI GC'TnA_GC Cuts at: 0 4 982 1551 Size: 4 978 569 Fragments arranged by size: 978 569 4 Bme1580I G_kGCm'C Cuts at: 0 1188 1551 Size: 1188 363 BmrI ACTGGGnnnn_n' Cuts at: 0 111 297 1264 1551 Size: 111 186 967 287 Fragments arranged by size: 967 287 186 111 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 315 348 1551 Size: 315 33 1203 Fragments arranged by size: 1203 315 33 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 294 595 1153 1458 1551 Size: 294 301 558 305 93 Fragments arranged by size: 558 305 301 294 93 BsaI GGTCTCn'nnnn_ Cuts at: 0 524 1431 1551 Size: 524 907 120 Fragments arranged by size: 907 524 120 BsaAI yAC'GTr Cuts at: 0 1026 1551 Size: 1026 525 BsaHI Gr'CG_yC Cuts at: 0 1214 1551 Size: 1214 337 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 568 598 1273 1276 1303 1306 1453 1483 Size: 568 30 675 3 27 3 147 30 Cuts at: 1483 1525 1551 Size: 42 26 Fragments arranged by size: 675 568 147 42 30 30 27 26 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1281 1551 Size: 1281 270 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1048 1551 Size: 1048 503 BsiEI CG_ry'CG Cuts at: 0 38 77 1551 Size: 38 39 1474 Fragments arranged by size: 1474 39 38 BsiHKAI G_wGCw'C Cuts at: 0 10 1025 1551 Size: 10 1015 526 Fragments arranged by size: 1015 526 10 BsmI GAATG_Cn' Cuts at: 0 567 1551 Size: 567 984 Bsp1286I G_dGCh'C Cuts at: 0 10 329 1025 1158 1188 1551 Size: 10 319 696 133 30 363 Fragments arranged by size: 696 363 319 133 30 10 BspHI T'CATG_A Cuts at: 0 92 233 1551 Size: 92 141 1318 Fragments arranged by size: 1318 141 92 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 235 619 1493 1551 Size: 235 384 874 58 Fragments arranged by size: 874 384 235 58 BsrGI T'GTAC_A Cuts at: 0 446 859 1551 Size: 446 413 692 Fragments arranged by size: 692 446 413 BstEII G'GTnAC_C Cuts at: 0 626 781 1551 Size: 626 155 770 Fragments arranged by size: 770 626 155 BstXI CCAn_nnnn'nTGG Cuts at: 0 1075 1551 Size: 1075 476 BstYI r'GATC_y Cuts at: 0 458 1551 Size: 458 1093 BtgI C'CryG_G Cuts at: 0 32 996 1332 1551 Size: 32 964 336 219 Fragments arranged by size: 964 336 219 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 40 1551 Size: 40 1511 BtsI GCAGTG_nn' Cuts at: 0 801 1551 Size: 801 750 EaeI y'GGCC_r Cuts at: 0 35 74 1551 Size: 35 39 1477 Fragments arranged by size: 1477 39 35 EagI C'GGCC_G Cuts at: 0 35 74 1551 Size: 35 39 1477 Fragments arranged by size: 1477 39 35 EarI CTCTTCn'nnn_ Cuts at: 0 284 1309 1551 Size: 284 1025 242 Fragments arranged by size: 1025 284 242 EcoICRI GAG'CTC Cuts at: 0 8 1551 Size: 8 1543 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 294 595 1030 1153 1458 1551 Size: 294 301 435 123 305 93 Fragments arranged by size: 435 305 301 294 123 93 EcoNI CCTnn'n_nnAGG Cuts at: 0 1459 1551 Size: 1459 92 EcoO109I rG'GnC_Cy Cuts at: 0 160 1123 1184 1185 1464 1551 Size: 160 963 61 1 279 87 Fragments arranged by size: 963 279 160 87 61 1 HaeII r_GCGC'y Cuts at: 0 57 1217 1252 1481 1551 Size: 57 1160 35 229 70 Fragments arranged by size: 1160 229 70 57 35 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 74 106 443 475 568 600 1273 1305 Size: 74 32 337 32 93 32 673 32 Cuts at: 1305 1330 1343 1362 1375 1453 1485 1525 1551 Size: 25 13 19 13 78 32 40 26 Fragments arranged by size: 673 337 93 78 74 40 32 32 32 32 32 26 25 19 13 13 HincII GTy'rAC Cuts at: 0 1093 1551 Size: 1093 458 KasI G'GCGC_C Cuts at: 0 1213 1551 Size: 1213 338 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 410 1551 Size: 410 1141 MspA1I CmG'CkG Cuts at: 0 34 978 1551 Size: 34 944 573 Fragments arranged by size: 944 573 34 NarI GG'CG_CC Cuts at: 0 1214 1551 Size: 1214 337 NcoI C'CATG_G Cuts at: 0 1332 1551 Size: 1332 219 NspI r_CATG'y Cuts at: 0 1344 1551 Size: 1344 207 PasI CC'CwG_GG Cuts at: 0 1120 1551 Size: 1120 431 PflMI CCAn_nnn'nTGG Cuts at: 0 650 1551 Size: 650 901 PfoI T'CCnGG_A Cuts at: 0 1510 1551 Size: 1510 41 PmlI CAC'GTG Cuts at: 0 1026 1551 Size: 1026 525 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1384 1416 1551 Size: 1384 32 135 Fragments arranged by size: 1384 135 32 PpuMI rG'GwC_Cy Cuts at: 0 160 1464 1551 Size: 160 1304 87 Fragments arranged by size: 1304 160 87 PspOMI G'GGCC_C Cuts at: 0 1184 1551 Size: 1184 367 PstI C_TGCA'G Cuts at: 0 1142 1460 1551 Size: 1142 318 91 Fragments arranged by size: 1142 318 91 RsrII CG'GwC_CG Cuts at: 0 65 1551 Size: 65 1486 SacI G_AGCT'C Cuts at: 0 10 1551 Size: 10 1541 SacII CC_GC'GG Cuts at: 0 35 1551 Size: 35 1516 SanDI GG'GwC_CC Cuts at: 0 160 1551 Size: 160 1391 SbfI CC_TGCA'GG Cuts at: 0 1460 1551 Size: 1460 91 ScaI AGT'ACT Cuts at: 0 307 1551 Size: 307 1244 SfcI C'TryA_G Cuts at: 0 973 1138 1284 1456 1551 Size: 973 165 146 172 95 Fragments arranged by size: 973 172 165 146 95 SfiI GGCCn_nnn'nGGCC Cuts at: 0 43 1551 Size: 43 1508 SfoI GGC'GCC Cuts at: 0 1215 1551 Size: 1215 336 SmaI CCC'GGG Cuts at: 0 13 1551 Size: 13 1538 SmlI C'TyrA_G Cuts at: 0 438 1551 Size: 438 1113 SpeI A'CTAG_T Cuts at: 0 685 1551 Size: 685 866 StuI AGG'CCT Cuts at: 0 254 1551 Size: 254 1297 StyI C'CwwG_G Cuts at: 0 875 1074 1332 1551 Size: 875 199 258 219 Fragments arranged by size: 875 258 219 199 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 800 1551 Size: 800 751 TatI w'GTAC_w Cuts at: 0 305 446 859 1551 Size: 305 141 413 692 Fragments arranged by size: 692 413 305 141 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 625 657 832 864 1551 Size: 625 32 175 32 687 Fragments arranged by size: 687 625 175 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1438 1551 Size: 1438 113 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 773 1551 Size: 773 778 XmaI C'CCGG_G Cuts at: 0 11 1551 Size: 11 1540 Enzymes that do cut: AarI AclI AcuI AfeI AflII AhdI AlwNI ApaI AvaI BanI BanII BbeI BbsI BglI BglII BlpI Bme1580I BmrI BplI BpmI BsaI BsaAI BsaHI BsaXI BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrGI BstEII BstXI BstYI BtgI BtgZI BtsI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI MmeI MspA1I NarI NcoI NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI RsrII SacI SacII SanDI SbfI ScaI SfcI SfiI SfoI SmaI SmlI SpeI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AflIII AgeI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BmgBI BmtI Bpu10I BpuEI BsaBI BsaWI BseYI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EciI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MscI MslI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PshAI PsiI PspXI PsrI PvuI PvuII SalI SapI SexAI SgfI SgrAI SnaBI SphI SrfI SspI SwaI XbaI XhoI XmnI ZraI