(Circular) (Six-base) MAPSORT of: pFN21AB3774.seq Check: 6844 from: 1 to: 5755 pFN21AB3774 5755 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2328 3565 278 Size: 53 83 186 936 792 1237 2468 Fragments arranged by size: 2468 1237 936 792 186 83 53 AccI GT'mk_AC Cuts at: 1937 3042 3098 1937 Size: 1105 56 4594 Fragments arranged by size: 4594 1105 56 Acc65I G'GTAC_C Cuts at: 2829 3076 2829 Size: 247 5508 AclI AA'CG_TT Cuts at: 1563 3054 3882 4255 1563 Size: 1491 828 373 3063 Fragments arranged by size: 3063 1491 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3818 5202 5470 1632 Size: 2186 1384 268 1917 Fragments arranged by size: 2186 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5558 AflIII A'CryG_T Cuts at: 1203 2889 4654 5631 1203 Size: 1686 1765 977 1327 Fragments arranged by size: 1765 1686 1327 977 AgeI A'CCGG_T Cuts at: 2946 4586 2946 Size: 1640 4115 AhdI GACnn_n'nnGTC Cuts at: 4484 4484 Size: 5755 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5755 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5755 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2381 2413 2381 Size: 32 5723 AlwNI CAG_nnn'CTG Cuts at: 3498 5070 3498 Size: 1572 4183 ApaI G_GGCC'C Cuts at: 2128 2128 Size: 5755 ApaLI G'TGCA_C Cuts at: 2978 3812 4968 2978 Size: 834 1156 3765 Fragments arranged by size: 3765 1156 834 ApoI r'AATT_y Cuts at: 1493 3064 3290 3442 5551 1493 Size: 1571 226 152 2109 1697 Fragments arranged by size: 2109 1697 1571 226 152 AseI AT'TA_AT Cuts at: 160 1032 4309 160 Size: 872 3277 1606 Fragments arranged by size: 3277 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 AvaI C'yCGr_G Cuts at: 1870 1943 2200 2298 2476 3080 1870 Size: 73 257 98 178 604 4545 Fragments arranged by size: 4545 604 257 178 98 73 BamHI G'GATC_C Cuts at: 3085 3452 3085 Size: 367 5388 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2155 2243 2548 2653 2829 Size: 325 217 627 368 88 305 105 176 Cuts at: 2829 2925 3076 4531 618 Size: 96 151 1455 1842 Fragments arranged by size: 1842 1455 627 368 325 305 217 176 151 105 96 88 BanII G_rGCy'C Cuts at: 1391 2128 2495 3074 1391 Size: 737 367 579 4072 Fragments arranged by size: 4072 737 579 367 BbeI G_GCGC'C Cuts at: 2552 2552 Size: 5755 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2975 928 Size: 971 1076 3708 Fragments arranged by size: 3708 1076 971 BbvCI CC'TCA_GC Cuts at: 2183 2183 Size: 5755 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2225 2259 3946 3980 1903 Size: 34 288 34 1687 34 3678 Fragments arranged by size: 3678 1687 288 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3647 4857 3647 Size: 1210 4545 BclI T'GATC_A Cuts at: 1548 1548 Size: 5755 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4366 136 Size: 107 122 71 631 605 2694 1525 Fragments arranged by size: 2694 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2769 5750 2769 Size: 2981 2774 BlpI GC'TnA_GC Cuts at: 3172 3172 Size: 5755 Bme1580I G_kGCm'C Cuts at: 1790 2128 2160 2523 2658 2982 3816 4972 1790 Size: 338 32 363 135 324 834 1156 2573 Fragments arranged by size: 2573 1156 834 363 338 324 135 32 BmgBI CAC'GTC Cuts at: 1318 2512 1318 Size: 1194 4561 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2350 2723 2944 Size: 416 53 83 399 24 933 373 221 Cuts at: 2944 3486 4444 442 Size: 542 958 1753 Fragments arranged by size: 1753 958 933 542 416 399 373 221 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5755 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2215 2215 Size: 5755 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2408 4415 1239 Size: 1169 2007 2579 Fragments arranged by size: 2579 2007 1169 Bpu10I CC'TnA_GC Cuts at: 2183 2183 Size: 5755 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3880 4745 5043 3880 Size: 865 298 4592 Fragments arranged by size: 4592 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4418 882 Size: 617 2919 2219 Fragments arranged by size: 2919 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3546 493 Size: 711 2342 2702 Fragments arranged by size: 2702 2342 711 BsaBI GATnn'nnATC Cuts at: 5749 5749 Size: 5755 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2325 2549 3562 3944 Size: 53 83 186 936 792 224 1013 382 Cuts at: 3944 4579 275 Size: 635 1451 Fragments arranged by size: 1451 1013 936 792 635 382 224 186 83 53 BsaWI w'CCGG_w Cuts at: 2320 2946 2964 4188 4586 4860 5007 5418 2320 Size: 626 18 1224 398 274 147 411 2657 Fragments arranged by size: 2657 1224 626 411 398 274 147 18 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2381 2411 1178 Size: 30 165 30 978 30 4522 Fragments arranged by size: 4522 978 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5755 BseYI C'CCAG_C Cuts at: 2779 4958 5454 2779 Size: 2179 496 3080 Fragments arranged by size: 3080 2179 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5182 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3205 3966 4994 1540 Size: 275 186 1204 761 1028 2301 Fragments arranged by size: 2301 1204 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2015 2982 3816 3901 4972 2015 Size: 967 834 85 1071 2798 Fragments arranged by size: 2798 1071 967 834 85 BsmI GAATG_Cn' Cuts at: 3266 3359 3266 Size: 93 5662 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2128 2160 2495 2523 2658 2982 Size: 399 225 113 32 335 28 135 324 Cuts at: 2982 3074 3816 3901 4972 1391 Size: 92 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 335 324 225 135 113 92 85 32 28 BspEI T'CCGG_A Cuts at: 2964 5418 2964 Size: 2454 3301 BspHI T'CATG_A Cuts at: 2532 3644 5374 2532 Size: 1112 1730 2913 Fragments arranged by size: 2913 1730 1112 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2493 2634 3110 844 Size: 1649 141 476 3489 Fragments arranged by size: 3489 1649 476 141 BsrBI CCG'CTC Cuts at: 1394 1823 3642 1394 Size: 429 1819 3507 Fragments arranged by size: 3507 1819 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4250 4424 65 Size: 1183 3002 174 1396 Fragments arranged by size: 3002 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2168 2240 2451 2946 4399 4586 1700 Size: 110 358 72 211 495 1453 187 2869 Fragments arranged by size: 2869 1453 495 358 211 187 110 72 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5755 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5755 BssSI C'ACGA_G Cuts at: 3815 4827 3815 Size: 1012 4743 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5755 BstBI TT'CG_AA Cuts at: 4559 4559 Size: 5755 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5755 BstYI r'GATC_y Cuts at: 1972 2311 2709 2769 3085 3452 3837 3854 5306 Size: 339 398 60 316 367 385 17 1452 Cuts at: 5306 5392 5404 5750 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 398 385 367 346 339 316 86 60 17 12 BtgI C'CryG_G Cuts at: 513 1065 2037 2463 2556 2700 2838 513 Size: 552 972 426 93 144 138 3430 Fragments arranged by size: 3430 972 552 426 144 138 93 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2250 487 Size: 683 56 1024 3992 Fragments arranged by size: 3992 1024 683 56 BtsI GCAGTG_nn' Cuts at: 3271 4065 4085 3271 Size: 794 20 4941 Fragments arranged by size: 4941 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5755 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5720 DraI TTT'AAA Cuts at: 3059 3059 Size: 5755 DraIII CAC_nnn'GTG Cuts at: 2146 2146 Size: 5755 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4762 809 Size: 507 3446 1802 Fragments arranged by size: 3446 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2097 3018 3202 Size: 54 1425 50 275 18 267 921 184 Cuts at: 3202 4091 5720 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 921 889 275 267 184 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3202 1537 Size: 275 1390 4090 Fragments arranged by size: 4090 1390 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2078 3685 1353 Size: 725 1607 3423 Fragments arranged by size: 3423 1607 725 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4342 4716 4862 387 Size: 1064 138 2753 374 146 1280 Fragments arranged by size: 2753 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2408 3818 4415 5202 5470 1239 Size: 393 776 1410 597 787 268 1524 Fragments arranged by size: 1524 1410 787 776 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2186 2186 Size: 5755 EcoO109I rG'GnC_Cy Cuts at: 2124 2125 2437 2665 2124 Size: 1 312 228 5214 Fragments arranged by size: 5214 312 228 1 EcoRI G'AATT_C Cuts at: 3064 3442 3064 Size: 378 5377 FspI TGC'GCA Cuts at: 4261 4261 Size: 5755 HaeII r_GCGC'y Cuts at: 1678 2182 2458 2552 2694 4902 1678 Size: 504 276 94 142 2208 2531 Fragments arranged by size: 2531 2208 504 276 142 94 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2527 2559 2803 2835 4396 Size: 32 135 32 955 32 244 32 1561 Cuts at: 4396 4428 4470 4502 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1561 955 244 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3099 3345 669 Size: 988 281 1161 246 3079 Fragments arranged by size: 3079 1161 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5755 HpaI GTT'AAC Cuts at: 3345 3345 Size: 5755 KasI G'GCGC_C Cuts at: 2548 2548 Size: 5755 KpnI G_GTAC'C Cuts at: 2833 3080 2833 Size: 247 5508 MfeI C'AATT_G Cuts at: 3354 3354 Size: 5755 MluI A'CGCG_T Cuts at: 5631 5631 Size: 5755 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2274 4869 5053 1305 Size: 969 2595 184 2007 Fragments arranged by size: 2595 2007 969 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2099 3020 10 Size: 54 1425 343 267 921 2745 Fragments arranged by size: 2745 1425 921 343 267 54 MslI CAynn'nnrTG Cuts at: 518 1166 2537 2843 2874 3011 3713 4072 4231 Size: 648 1371 306 31 137 702 359 159 Cuts at: 4231 518 Size: 2042 Fragments arranged by size: 2042 1371 702 648 359 306 159 137 31 MspA1I CmG'CkG Cuts at: 1606 2226 3171 3848 4996 5241 1606 Size: 620 945 677 1148 245 2120 Fragments arranged by size: 2120 1148 945 677 620 245 NaeI GCC'GGC Cuts at: 2170 2453 2170 Size: 283 5472 NarI GG'CG_CC Cuts at: 2549 2549 Size: 5755 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5203 NdeI CA'TA_TG Cuts at: 387 387 Size: 5755 NgoMIV G'CCGG_C Cuts at: 2168 2451 2168 Size: 283 5472 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5755 NotI GC'GGCC_GC Cuts at: 3202 3202 Size: 5755 NspI r_CATG'y Cuts at: 1132 1229 2873 3016 3113 4658 1132 Size: 97 1644 143 97 1545 2229 Fragments arranged by size: 2229 1644 1545 143 97 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5755 PciI A'CATG_T Cuts at: 4654 4654 Size: 5755 PflMI CCAn_nnn'nTGG Cuts at: 1108 2095 1108 Size: 987 4768 PfoI T'CCnGG_A Cuts at: 2433 2667 2433 Size: 234 5521 PmeI GTTT'AAAC Cuts at: 3059 3059 Size: 5755 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2381 2413 3824 3856 5337 5369 2381 Size: 32 1411 32 1481 32 2767 Fragments arranged by size: 2767 1481 1411 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2437 2665 2437 Size: 228 5527 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5755 PsiI TTA'TAA Cuts at: 3325 3325 Size: 5755 PspOMI G'GGCC_C Cuts at: 2124 2124 Size: 5755 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2933 2965 2933 Size: 32 5723 PstI C_TGCA'G Cuts at: 830 2178 2352 3107 830 Size: 1348 174 755 3478 Fragments arranged by size: 3478 1348 755 174 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5755 SalI G'TCGA_C Cuts at: 1936 3097 1936 Size: 1161 4594 SanDI GG'GwC_CC Cuts at: 2437 2437 Size: 5755 SapI GCTCTTCn'nnn_ Cuts at: 2078 2078 Size: 5755 SbfI CC_TGCA'GG Cuts at: 3107 3107 Size: 5755 ScaI AGT'ACT Cuts at: 4003 5715 4003 Size: 1712 4043 SfcI C'TryA_G Cuts at: 826 1045 2174 2348 3103 4238 4919 5110 826 Size: 219 1129 174 755 1135 681 191 1471 Fragments arranged by size: 1471 1135 1129 755 681 219 191 174 SfoI GGC'GCC Cuts at: 2550 2550 Size: 5755 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5755 SmaI CCC'GGG Cuts at: 1872 3082 1872 Size: 1210 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3859 4760 5022 820 Size: 197 926 1916 901 262 1553 Fragments arranged by size: 1916 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3546 493 Size: 3053 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5755 SphI G_CATG'C Cuts at: 1132 3113 1132 Size: 1981 3774 SspI AAT'ATT Cuts at: 5 52 3679 5 Size: 47 3627 2081 Fragments arranged by size: 3627 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5755 StyI C'CwwG_G Cuts at: 513 1065 3021 3194 513 Size: 552 1956 173 3074 Fragments arranged by size: 3074 1956 552 173 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2606 3782 3799 3952 4137 4582 325 Size: 1255 1026 1176 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1176 1026 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4001 5713 96 Size: 274 80 33 51 1443 2024 1712 138 Fragments arranged by size: 2024 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2310 2342 2663 2695 5715 5747 2310 Size: 32 321 32 3020 32 2318 Fragments arranged by size: 3020 2318 321 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5755 XbaI T'CTAG_A Cuts at: 3091 3091 Size: 5755 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2383 2408 2845 2383 Size: 25 437 5293 Fragments arranged by size: 5293 437 25 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5755 XmaI C'CCGG_G Cuts at: 1870 3080 1870 Size: 1210 4545 XmnI GAAnn'nnTTC Cuts at: 2396 2738 3884 2396 Size: 342 1146 4267 Fragments arranged by size: 4267 1146 342 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2326 3563 276 Size: 53 83 186 936 792 1237 2468 Fragments arranged by size: 2468 1237 936 792 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PvuII RsrII SacI SacII SexAI SfiI SrfI SwaI