(Circular) (Six-base) MAPSORT of: pFN21AB3738.seq Check: 3655 from: 1 to: 5776 pFN21AB3738 5776 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3586 278 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3119 1937 Size: 1182 4594 Acc65I G'GTAC_C Cuts at: 3097 3097 Size: 5776 AclI AA'CG_TT Cuts at: 1563 3903 4276 1563 Size: 2340 373 3063 Fragments arranged by size: 3063 2340 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3839 5223 5491 1632 Size: 2207 1384 268 1917 Fragments arranged by size: 2207 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5579 AflIII A'CryG_T Cuts at: 1203 2910 4675 5652 1203 Size: 1707 1765 977 1327 Fragments arranged by size: 1765 1707 1327 977 AgeI A'CCGG_T Cuts at: 4607 4607 Size: 5776 AhdI GACnn_n'nnGTC Cuts at: 2528 4505 2528 Size: 1977 3799 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5776 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5776 AlwNI CAG_nnn'CTG Cuts at: 3519 5091 3519 Size: 1572 4204 ApaLI G'TGCA_C Cuts at: 2045 2752 3833 4989 2045 Size: 707 1081 1156 2832 Fragments arranged by size: 2832 1156 1081 707 ApoI r'AATT_y Cuts at: 1493 2179 2315 3085 3311 3463 5572 1493 Size: 686 136 770 226 152 2109 1697 Fragments arranged by size: 2109 1697 770 686 226 152 136 AseI AT'TA_AT Cuts at: 160 1032 4330 160 Size: 872 3298 1606 Fragments arranged by size: 3298 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 AvaI C'yCGr_G Cuts at: 1870 1943 2301 3101 1870 Size: 73 358 800 4545 Fragments arranged by size: 4545 800 358 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2832 2865 2832 Size: 33 5743 BamHI G'GATC_C Cuts at: 2487 3106 3473 2487 Size: 619 367 4790 Fragments arranged by size: 4790 619 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2459 3097 4552 618 Size: 325 217 627 672 638 1455 1842 Fragments arranged by size: 1842 1455 672 638 627 325 217 BanII G_rGCy'C Cuts at: 1391 2690 2834 3095 1391 Size: 1299 144 261 4072 Fragments arranged by size: 4072 1299 261 144 BbeI G_GCGC'C Cuts at: 2463 2463 Size: 5776 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4805 BbvCI CC'TCA_GC Cuts at: 2114 2114 Size: 5776 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2276 2310 2914 2948 3967 4001 1903 Size: 34 339 34 604 34 1019 34 3678 Fragments arranged by size: 3678 1019 604 339 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3668 4878 3668 Size: 1210 4566 BclI T'GATC_A Cuts at: 1548 1548 Size: 5776 BfrBI ATG'CAT Cuts at: 2122 2122 Size: 5776 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4387 136 Size: 107 122 71 631 605 337 2378 1525 Fragments arranged by size: 2378 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2673 5771 2673 Size: 3098 2678 BlpI GC'TnA_GC Cuts at: 3193 3193 Size: 5776 Bme1580I G_kGCm'C Cuts at: 1790 2049 2756 3837 4993 1790 Size: 259 707 1081 1156 2573 Fragments arranged by size: 2573 1156 1081 707 259 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5776 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2167 3054 3507 Size: 416 53 83 399 24 750 887 453 Cuts at: 3507 4465 442 Size: 958 1753 Fragments arranged by size: 1753 958 887 750 453 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5776 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2090 2654 3008 3020 4436 1239 Size: 851 564 354 12 1416 2579 Fragments arranged by size: 2579 1416 851 564 354 12 Bpu10I CC'TnA_GC Cuts at: 2114 2114 Size: 5776 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2583 3032 3901 4766 5064 2583 Size: 449 869 865 298 3295 Fragments arranged by size: 3295 869 865 449 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4439 882 Size: 617 2940 2219 Fragments arranged by size: 2940 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2913 3567 493 Size: 711 1709 654 2702 Fragments arranged by size: 2702 1709 711 654 BsaBI GATnn'nnATC Cuts at: 5770 5770 Size: 5776 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2460 3583 3965 4600 Size: 53 83 186 936 927 1123 382 635 Cuts at: 4600 275 Size: 1451 Fragments arranged by size: 1451 1123 936 927 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4209 4607 4881 5028 5439 4209 Size: 398 274 147 411 4546 Fragments arranged by size: 4546 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5551 Fragments arranged by size: 5551 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5776 BseYI C'CCAG_C Cuts at: 2206 2546 2722 2827 4979 5475 2206 Size: 340 176 105 2152 496 2507 Fragments arranged by size: 2507 2152 496 340 176 105 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5203 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3226 3987 5015 1540 Size: 275 186 1225 761 1028 2301 Fragments arranged by size: 2301 1225 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2049 2299 2756 3837 3922 4993 2049 Size: 250 457 1081 85 1071 2832 Fragments arranged by size: 2832 1081 1071 457 250 85 BsmI GAATG_Cn' Cuts at: 2122 3287 3380 2122 Size: 1165 93 4518 Fragments arranged by size: 4518 1165 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2299 2690 2756 2834 3095 3837 Size: 399 259 250 391 66 78 261 742 Cuts at: 3837 3922 4993 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 391 261 259 250 85 78 66 BspEI T'CCGG_A Cuts at: 5439 5439 Size: 5776 BspHI T'CATG_A Cuts at: 3665 5395 3665 Size: 1730 4046 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3131 844 Size: 2287 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3663 1394 Size: 429 1840 3507 Fragments arranged by size: 3507 1840 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2592 4271 4445 65 Size: 1183 1344 1679 174 1396 Fragments arranged by size: 1679 1396 1344 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2394 4420 4607 1700 Size: 110 584 2026 187 2869 Fragments arranged by size: 2869 2026 584 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5776 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5776 BssSI C'ACGA_G Cuts at: 2573 2813 3836 4848 2573 Size: 240 1023 1012 3501 Fragments arranged by size: 3501 1023 1012 240 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5776 BstBI TT'CG_AA Cuts at: 4580 4580 Size: 5776 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5776 BstXI CCAn_nnnn'nTGG Cuts at: 2206 2587 2206 Size: 381 5395 BstYI r'GATC_y Cuts at: 1972 2487 2673 3106 3473 3858 3875 5327 5413 Size: 515 186 433 367 385 17 1452 86 Cuts at: 5413 5425 5771 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 515 433 385 367 346 186 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2691 2691 Size: 5776 BtgI C'CryG_G Cuts at: 513 1065 2004 2199 513 Size: 552 939 195 4090 Fragments arranged by size: 4090 939 552 195 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5037 Fragments arranged by size: 5037 683 56 BtsI GCAGTG_nn' Cuts at: 3292 4086 4106 3292 Size: 794 20 4962 Fragments arranged by size: 4962 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5776 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5741 DraI TTT'AAA Cuts at: 3019 3080 3019 Size: 61 5715 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2617 4783 809 Size: 507 1301 2166 1802 Fragments arranged by size: 2166 1802 1301 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 2577 2915 Size: 54 1425 50 275 18 186 561 338 Cuts at: 2915 3223 4112 5741 8 Size: 308 889 1629 43 Fragments arranged by size: 1629 1425 889 561 338 308 275 186 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3223 1537 Size: 275 1411 4090 Fragments arranged by size: 4090 1411 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3706 1353 Size: 2353 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2523 4363 4737 4883 387 Size: 1064 138 934 1840 374 146 1280 Fragments arranged by size: 1840 1280 1064 934 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 2654 3008 3020 3839 4436 5223 Size: 393 458 564 354 12 819 597 787 Cuts at: 5223 5491 1239 Size: 268 1524 Fragments arranged by size: 1524 819 787 597 564 458 393 354 268 12 EcoNI CCTnn'n_nnAGG Cuts at: 2091 2091 Size: 5776 EcoO109I rG'GnC_Cy Cuts at: 2530 2808 2530 Size: 278 5498 EcoRI G'AATT_C Cuts at: 2315 3085 3463 2315 Size: 770 378 4628 Fragments arranged by size: 4628 770 378 EcoRV GAT'ATC Cuts at: 2884 2884 Size: 5776 FspI TGC'GCA Cuts at: 4282 4282 Size: 5776 HaeII r_GCGC'y Cuts at: 1678 2463 4923 1678 Size: 785 2460 2531 Fragments arranged by size: 2531 2460 785 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2514 2546 4417 4449 4491 Size: 32 135 32 942 32 1871 32 42 Cuts at: 4491 4523 1373 Size: 32 2626 Fragments arranged by size: 2626 1871 942 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3120 3366 669 Size: 988 281 1182 246 3079 Fragments arranged by size: 3079 1182 988 281 246 HindIII A'AGCT_T Cuts at: 748 2217 2429 748 Size: 1469 212 4095 Fragments arranged by size: 4095 1469 212 HpaI GTT'AAC Cuts at: 3366 3366 Size: 5776 KasI G'GCGC_C Cuts at: 2459 2459 Size: 5776 KpnI G_GTAC'C Cuts at: 3101 3101 Size: 5776 MfeI C'AATT_G Cuts at: 3375 3375 Size: 5776 MluI A'CGCG_T Cuts at: 5652 5652 Size: 5776 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2110 4890 5074 1305 Size: 805 2780 184 2007 Fragments arranged by size: 2780 2007 805 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2579 2917 10 Size: 54 1425 343 747 338 2869 Fragments arranged by size: 2869 1425 747 343 338 54 MslI CAynn'nnrTG Cuts at: 518 1166 3734 4093 4252 518 Size: 648 2568 359 159 2042 Fragments arranged by size: 2568 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2722 3192 3869 5017 5262 1606 Size: 1116 470 677 1148 245 2120 Fragments arranged by size: 2120 1148 1116 677 470 245 NarI GG'CG_CC Cuts at: 2460 2460 Size: 5776 NcoI C'CATG_G Cuts at: 513 1065 2004 2199 513 Size: 552 939 195 4090 Fragments arranged by size: 4090 939 552 195 NdeI CA'TA_TG Cuts at: 387 387 Size: 5776 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5776 NotI GC'GGCC_GC Cuts at: 3223 3223 Size: 5776 NsiI A_TGCA'T Cuts at: 2124 2124 Size: 5776 NspI r_CATG'y Cuts at: 1132 1229 2864 3134 4679 1132 Size: 97 1635 270 1545 2229 Fragments arranged by size: 2229 1635 1545 270 97 PasI CC'CwG_GG Cuts at: 1348 2518 1348 Size: 1170 4606 PciI A'CATG_T Cuts at: 4675 4675 Size: 5776 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5776 PfoI T'CCnGG_A Cuts at: 2871 2871 Size: 5776 PmeI GTTT'AAAC Cuts at: 3080 3080 Size: 5776 PmlI CAC'GTG Cuts at: 2913 2913 Size: 5776 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3845 3877 5358 5390 3845 Size: 32 1481 32 4231 Fragments arranged by size: 4231 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2530 2808 2530 Size: 278 5498 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5776 PsiI TTA'TAA Cuts at: 3346 3346 Size: 5776 PstI C_TGCA'G Cuts at: 830 2501 3128 830 Size: 1671 627 3478 Fragments arranged by size: 3478 1671 627 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5776 PvuII CAG'CTG Cuts at: 2722 2722 Size: 5776 RsrII CG'GwC_CG Cuts at: 2051 2051 Size: 5776 SalI G'TCGA_C Cuts at: 1936 3118 1936 Size: 1182 4594 SanDI GG'GwC_CC Cuts at: 2808 2808 Size: 5776 SbfI CC_TGCA'GG Cuts at: 2501 3128 2501 Size: 627 5149 ScaI AGT'ACT Cuts at: 4024 5736 4024 Size: 1712 4064 SexAI A'CCwGG_T Cuts at: 2266 2266 Size: 5776 SfcI C'TryA_G Cuts at: 826 1045 2497 3124 4259 4940 5131 826 Size: 219 1452 627 1135 681 191 1471 Fragments arranged by size: 1471 1452 1135 681 627 219 191 SfoI GGC'GCC Cuts at: 2461 2461 Size: 5776 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5776 SmaI CCC'GGG Cuts at: 1872 3103 1872 Size: 1231 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2598 3047 3880 4781 5043 820 Size: 197 926 655 449 833 901 262 1553 Fragments arranged by size: 1553 926 901 833 655 449 262 197 SnaBI TAC'GTA Cuts at: 493 3567 493 Size: 3074 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5776 SphI G_CATG'C Cuts at: 1132 2864 3134 1132 Size: 1732 270 3774 Fragments arranged by size: 3774 1732 270 SspI AAT'ATT Cuts at: 5 52 3700 5 Size: 47 3648 2081 Fragments arranged by size: 3648 2081 47 StuI AGG'CCT Cuts at: 1517 2768 3005 1517 Size: 1251 237 4288 Fragments arranged by size: 4288 1251 237 StyI C'CwwG_G Cuts at: 513 1065 2004 2199 2643 2679 3215 513 Size: 552 939 195 444 36 536 3074 Fragments arranged by size: 3074 939 552 536 444 195 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2987 3803 3820 3973 4158 4603 325 Size: 1255 1407 816 17 153 185 445 1498 Fragments arranged by size: 1498 1407 1255 816 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4022 5734 96 Size: 274 80 33 51 1443 2045 1712 138 Fragments arranged by size: 2045 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5736 5768 5736 Size: 32 5744 Tth111I GACn'n_nGTC Cuts at: 1316 2441 1316 Size: 1125 4651 XbaI T'CTAG_A Cuts at: 3112 3112 Size: 5776 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2875 2875 Size: 5776 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5776 XmaI C'CCGG_G Cuts at: 1870 3101 1870 Size: 1231 4545 XmnI GAAnn'nnTTC Cuts at: 3905 3905 Size: 5776 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3584 276 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BplI BsiWI BsmBI BstZ17I DraIII EcoICRI FalI FseI FspAI NaeI NgoMIV NruI PacI PspOMI PspXI PsrI SacI SacII SapI SfiI SrfI SwaI