Restriction map for
pFN21AE3602
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# NO CUT ENZYMES ( 118 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AgeI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AvrII
BamHI BbeI BbrPI BciVI BclI
BfaI BfrI BlnI BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KasI KpnI MaeI
MamI MfeI MluI MroI MseI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SbfI ScaI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp509I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 91 GACGTC GACGT^C
AccI 703 GTCTAC GT^[AC][GT]AC
AcyI 91 GACGTC G[GA]^CG[CT]C
AflIII 149 ACACGT A^C[GA][CT]GT
AhdI 636 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1341 GATCC GGATC(4/5)
ApaI 1175 GGGCCC GGGCC^C
AspEI 636 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 736 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1364 TGCGCA TGC^GCA
BbsI 997 GTCTTC GAAGAC(2/6)
BbvCI 649 GCTGAGG CCTCAGC(-5/-2)
BglII 933 AGATCT A^GATCT
BlpI 1144 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1144 GCTCAGC GC^T[ACGT]AGC
BpuAI 997 GTCTTC GAAGAC(2/6)
BsaHI 91 GACGTC G[GA]^CG[CT]C
BseRI 960 CTCCTC GAGGAG(10/8)
BsgI 1213 GTGCAG GTGCAG(16/14)
BspMI 524 ACCTGC ACCTGC(4/8)
BssHII 1251 GCGCGC G^CGCGC
BstAPI 1215 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 596 CCATGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 933 AGATCT [GA]^GATC[CT]
CelII 1144 GCTCAGC GC^T[ACGT]AGC
DraII 740 AGGTCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 636 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 297 CCTCGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 740 AGGTCCT [GA]G^G[ACGT]CC[CT]
FspI 1364 TGCGCA TGC^GCA
Hin1I 91 GACGTC G[GA]^CG[CT]C
MflI 933 AGATCT [GA]^GATC[CT]
NspI 1319 ACATGC [GA]CATG^[CT]
PflFI 736 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1009 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 740 AGGTCCT [GA]G^G[AT]CC[CT]
SexAI 737 ACCAGGT A^CC[AT]GGT
SmlI 112 CTCAAG C^T[CT][GA]AG
StuI 401 AGGCCT AGG^CCT
Tth111I 736 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1009 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 933 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
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Alw44I 467 GTGCAC G^TGCAC
1456 GTGCAC
ApaLI 467 GTGCAC G^TGCAC
1456 GTGCAC
BalI 758 TGGCCA TGG^CCA
1088 TGGCCA
BanI 610 GGTGCC G^G[CT][GA]CC
1161 GGTGCC
BcgI 407 CGACCAGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1073 CGATGGGGCTGC
BglI 53 GCCTCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1177 GCCCGCCTGGC
BsmAI 330 GAGAC GTCTC(1/5)
634 GAGAC
BsmFI 583 GGGAC GGGAC(10/14)
79 GTCCC
EcoT14I 596 CCATGG C^C[AT][AT]GG
854 CCATGG
HaeII 306 GGCGCT [GA]GCGC^[CT]
1308 GGCGCT
HphI 510 GGTGA GGTGA(8/7)
844 TCACC
KspI 47 CCGCGG CCGC^GG
1286 CCGCGG
MaeII 92 ACGT A^CGT
151 ACGT
MluNI 758 TGGCCA TGG^CCA
1088 TGGCCA
MscI 758 TGGCCA TGG^CCA
1088 TGGCCA
NaeI 58 GCCGGC GCC^GGC
174 GCCGGC
NcoI 596 CCATGG C^CATGG
854 CCATGG
NgoMIV 58 GCCGGC G^CCGGC
174 GCCGGC
PstI 1096 CTGCAG CTGCA^G
1295 CTGCAG
SacII 47 CCGCGG CCGC^GG
1286 CCGCGG
SapI 948 GCTCTTC GCTCTTC(1/4)
1407 GCTCTTC
SfcI 1096 CTGCAG C^T[GA][CT]AG
1295 CTGCAG
SfiI 52 GGCCTCGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1176 GGCCCGCCTGGCC
StyI 596 CCATGG C^C[AT][AT]GG
854 CCATGG
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
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AccII AciI AfaI AluI AlwNI
AspHI AvaI AvaII BanII BbvI
BcnI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DsaI EaeI EarI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
ItaI Ksp632I MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TseI
Tsp45I TspRI TthHB8I XcmI