(Circular) (Six-base) MAPSORT of: pFN21AE3602.seq Check: 4938 from: 1 to: 6190 pFN21AE3602 6190 bp With 182 enzymes: SgfI * March 28, 2012 14:20 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2100 4000 278 Size: 53 83 186 936 564 1900 2468 Fragments arranged by size: 2468 1900 936 564 186 83 53 AccI GT'mk_AC Cuts at: 1937 2709 3533 1937 Size: 772 824 4594 Fragments arranged by size: 4594 824 772 Acc65I G'GTAC_C Cuts at: 3511 3511 Size: 6190 AclI AA'CG_TT Cuts at: 1563 4317 4690 1563 Size: 2754 373 3063 Fragments arranged by size: 3063 2754 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2300 2941 3370 4253 5637 5905 1632 Size: 668 641 429 883 1384 268 1917 Fragments arranged by size: 1917 1384 883 668 641 429 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5993 AflIII A'CryG_T Cuts at: 1203 2154 5089 6066 1203 Size: 951 2935 977 1327 Fragments arranged by size: 2935 1327 977 951 AgeI A'CCGG_T Cuts at: 5021 5021 Size: 6190 AhdI GACnn_n'nnGTC Cuts at: 2646 4919 2646 Size: 2273 3917 AleI CACnn'nnGTG Cuts at: 1166 2822 3302 1166 Size: 1656 480 4054 Fragments arranged by size: 4054 1656 480 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6190 AlwNI CAG_nnn'CTG Cuts at: 2226 2758 3020 3109 3226 3229 3393 3933 5505 Size: 532 262 89 117 3 164 540 1572 Cuts at: 5505 2226 Size: 2911 Fragments arranged by size: 2911 1572 540 532 262 164 117 89 3 ApaI G_GGCC'C Cuts at: 3184 3184 Size: 6190 ApaLI G'TGCA_C Cuts at: 2472 3461 4247 5403 2472 Size: 989 786 1156 3259 Fragments arranged by size: 3259 1156 989 786 ApoI r'AATT_y Cuts at: 1493 3499 3725 3877 5986 1493 Size: 2006 226 152 2109 1697 Fragments arranged by size: 2109 2006 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4744 160 Size: 872 3712 1606 Fragments arranged by size: 3712 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6190 AvaI C'yCGr_G Cuts at: 1870 1943 2485 2831 3162 3515 1870 Size: 73 542 346 331 353 4545 Fragments arranged by size: 4545 542 353 346 331 73 BamHI G'GATC_C Cuts at: 3520 3887 3520 Size: 367 5823 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2615 3166 3511 4966 618 Size: 325 217 627 828 551 345 1455 1842 Fragments arranged by size: 1842 1455 828 627 551 345 325 217 BanII G_rGCy'C Cuts at: 1391 2955 3151 3184 3509 1391 Size: 1564 196 33 325 4072 Fragments arranged by size: 4072 1564 325 196 33 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2995 928 Size: 971 1096 4123 Fragments arranged by size: 4123 1096 971 BbvCI CC'TCA_GC Cuts at: 2655 2655 Size: 6190 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2401 2435 3067 3101 4381 4415 1903 Size: 34 464 34 632 34 1280 34 3678 Fragments arranged by size: 3678 1280 632 464 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4082 5292 4082 Size: 1210 4980 BclI T'GATC_A Cuts at: 1548 1548 Size: 6190 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2064 3188 4801 Size: 107 122 71 631 605 392 1124 1613 Cuts at: 4801 136 Size: 1525 Fragments arranged by size: 1613 1525 1124 631 605 392 122 107 71 BglII A'GATC_T Cuts at: 2938 6185 2938 Size: 3247 2943 BlpI GC'TnA_GC Cuts at: 3150 3607 3150 Size: 457 5733 Bme1580I G_kGCm'C Cuts at: 1790 2328 2348 2476 2773 3184 3380 3465 4251 Size: 538 20 128 297 411 196 85 786 Cuts at: 4251 5407 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 786 538 411 297 196 128 85 20 BmgBI CAC'GTC Cuts at: 1318 2157 1318 Size: 839 5351 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2443 2603 3008 Size: 416 53 83 399 24 1026 160 405 Cuts at: 3008 3921 4879 442 Size: 913 958 1753 Fragments arranged by size: 1753 1026 958 913 416 405 399 160 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6190 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2236 2555 2735 2759 4850 1239 Size: 997 319 180 24 2091 2579 Fragments arranged by size: 2579 2091 997 319 180 24 Bpu10I CC'TnA_GC Cuts at: 2655 2655 Size: 6190 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2102 4315 5180 5478 2102 Size: 2213 865 298 2814 Fragments arranged by size: 2814 2213 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4853 882 Size: 617 3354 2219 Fragments arranged by size: 3354 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3981 493 Size: 711 2777 2702 Fragments arranged by size: 2777 2702 711 BsaBI GATnn'nnATC Cuts at: 6184 6184 Size: 6190 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2097 3997 4379 5014 Size: 53 83 186 936 564 1900 382 635 Cuts at: 5014 275 Size: 1451 Fragments arranged by size: 1900 1451 936 635 564 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4623 5021 5295 5442 5853 4623 Size: 398 274 147 411 4960 Fragments arranged by size: 4960 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3374 3404 1178 Size: 30 165 30 1971 30 3964 Fragments arranged by size: 3964 1971 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2956 1186 Size: 1770 4420 BseYI C'CCAG_C Cuts at: 2511 2556 2865 3020 3309 5393 5889 2511 Size: 45 309 155 289 2084 496 2812 Fragments arranged by size: 2812 2084 496 309 289 155 45 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3239 1163 Size: 573 1503 4114 Fragments arranged by size: 4114 1503 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3640 4401 5429 1540 Size: 275 186 1639 761 1028 2301 Fragments arranged by size: 2301 1639 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2424 2476 3465 4251 4336 5407 2424 Size: 52 989 786 85 1071 3207 Fragments arranged by size: 3207 1071 989 786 85 52 BsmI GAATG_Cn' Cuts at: 3701 3794 3701 Size: 93 6097 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2328 2348 2424 2476 2773 2955 3151 Size: 399 538 20 76 52 297 182 196 Cuts at: 3151 3184 3380 3465 3509 4251 4336 5407 1391 Size: 33 196 85 44 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 538 399 297 196 196 182 85 85 76 52 44 33 20 BspEI T'CCGG_A Cuts at: 5853 5853 Size: 6190 BspHI T'CATG_A Cuts at: 4079 5809 4079 Size: 1730 4460 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2538 3545 844 Size: 1694 1007 3489 Fragments arranged by size: 3489 1694 1007 BsrBI CCG'CTC Cuts at: 1394 1823 4077 1394 Size: 429 2254 3507 Fragments arranged by size: 3507 2254 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4685 4859 65 Size: 1183 3437 174 1396 Fragments arranged by size: 3437 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2063 2179 2565 4834 5021 1700 Size: 110 253 116 386 2269 187 2869 Fragments arranged by size: 2869 2269 386 253 187 116 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6190 BssHII G'CGCG_C Cuts at: 1925 3256 1925 Size: 1331 4859 BssSI C'ACGA_G Cuts at: 4250 5262 4250 Size: 1012 5178 BstAPI GCAn_nnn'nTGC Cuts at: 794 3226 794 Size: 2432 3758 BstBI TT'CG_AA Cuts at: 4994 4994 Size: 6190 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6190 BstXI CCAn_nnnn'nTGG Cuts at: 2608 2608 Size: 6190 BstYI r'GATC_y Cuts at: 1972 2938 3520 3887 4272 4289 5741 5827 5839 Size: 966 582 367 385 17 1452 86 12 Cuts at: 5839 6185 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 966 582 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2052 2579 2601 2782 2859 3090 Size: 552 939 48 527 22 181 77 231 Cuts at: 3090 3291 513 Size: 201 3412 Fragments arranged by size: 3412 939 552 527 231 201 181 77 48 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5451 Fragments arranged by size: 5451 683 56 BtsI GCAGTG_nn' Cuts at: 2024 2436 2923 3129 3297 3309 3706 4500 4520 Size: 412 487 206 168 12 397 794 20 Cuts at: 4520 2024 Size: 3694 Fragments arranged by size: 3694 794 487 412 397 206 168 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6190 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6155 DraI TTT'AAA Cuts at: 3494 3494 Size: 6190 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5197 809 Size: 507 3881 1802 Fragments arranged by size: 3881 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2046 2177 Size: 54 1425 50 275 18 177 39 131 Cuts at: 2177 2576 2692 2734 2763 2801 3093 3189 3637 Size: 399 116 42 29 38 292 96 448 Cuts at: 3637 4526 6155 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 448 399 292 275 177 131 116 96 54 50 43 42 39 38 29 18 EagI C'GGCC_G Cuts at: 1537 1812 3637 1537 Size: 275 1825 4090 Fragments arranged by size: 4090 1825 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2490 2960 3419 4120 1353 Size: 1137 470 459 701 3423 Fragments arranged by size: 3423 1137 701 470 459 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3457 4777 5151 5297 387 Size: 1064 138 1868 1320 374 146 1280 Fragments arranged by size: 1868 1320 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2236 2300 2555 2735 2759 2941 3370 Size: 393 604 64 255 180 24 182 429 Cuts at: 3370 4253 4850 5637 5905 1239 Size: 883 597 787 268 1524 Fragments arranged by size: 1524 883 787 604 597 429 393 268 255 182 180 64 24 EcoNI CCTnn'n_nnAGG Cuts at: 2306 2306 Size: 6190 EcoO109I rG'GnC_Cy Cuts at: 2746 2746 Size: 6190 EcoRI G'AATT_C Cuts at: 3499 3877 3499 Size: 378 5812 FspI TGC'GCA Cuts at: 3371 4696 3371 Size: 1325 4865 HaeII r_GCGC'y Cuts at: 1678 2315 3317 5337 1678 Size: 637 1002 2020 2531 Fragments arranged by size: 2531 2020 1002 637 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2632 2664 3133 3165 3374 Size: 32 135 32 1060 32 469 32 209 Cuts at: 3374 3406 4831 4863 4905 4937 1373 Size: 32 1425 32 42 32 2626 Fragments arranged by size: 2626 1425 1060 469 209 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3534 3780 669 Size: 988 281 1596 246 3079 Fragments arranged by size: 3079 1596 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6190 HpaI GTT'AAC Cuts at: 3780 3780 Size: 6190 KpnI G_GTAC'C Cuts at: 3515 3515 Size: 6190 MfeI C'AATT_G Cuts at: 3789 3789 Size: 6190 MluI A'CGCG_T Cuts at: 6066 6066 Size: 6190 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2275 5304 5488 1305 Size: 970 3029 184 2007 Fragments arranged by size: 3029 2007 970 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2765 3095 10 Size: 54 1425 343 933 330 3105 Fragments arranged by size: 3105 1425 933 343 330 54 MslI CAynn'nnrTG Cuts at: 518 1166 2159 2429 2822 3215 3302 4148 4507 Size: 648 993 270 393 393 87 846 359 Cuts at: 4507 4666 518 Size: 159 2042 Fragments arranged by size: 2042 993 846 648 393 393 359 270 159 87 MspA1I CmG'CkG Cuts at: 1606 2038 2054 2372 2654 2680 2869 3109 3154 Size: 432 16 318 282 26 189 240 45 Cuts at: 3154 3226 3293 3606 4283 5431 5676 1606 Size: 72 67 313 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 432 318 313 282 245 240 189 72 67 45 26 16 NaeI GCC'GGC Cuts at: 2065 2181 2065 Size: 116 6074 NcoI C'CATG_G Cuts at: 513 1065 2004 2601 2859 513 Size: 552 939 597 258 3844 Fragments arranged by size: 3844 939 597 552 258 NdeI CA'TA_TG Cuts at: 387 387 Size: 6190 NgoMIV G'CCGG_C Cuts at: 2063 2179 2063 Size: 116 6074 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6190 NotI GC'GGCC_GC Cuts at: 3637 3637 Size: 6190 NspI r_CATG'y Cuts at: 1132 1229 3328 3548 5093 1132 Size: 97 2099 220 1545 2229 Fragments arranged by size: 2229 2099 1545 220 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6190 PciI A'CATG_T Cuts at: 5089 5089 Size: 6190 PflMI CCAn_nnn'nTGG Cuts at: 1108 3020 1108 Size: 1912 4278 PfoI T'CCnGG_A Cuts at: 2100 2133 2100 Size: 33 6157 PmeI GTTT'AAAC Cuts at: 3494 3494 Size: 6190 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4259 4291 5772 5804 4259 Size: 32 1481 32 4645 Fragments arranged by size: 4645 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2746 2746 Size: 6190 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6190 PsiI TTA'TAA Cuts at: 3760 3760 Size: 6190 PspOMI G'GGCC_C Cuts at: 3180 3180 Size: 6190 PstI C_TGCA'G Cuts at: 830 3105 3304 3542 830 Size: 2275 199 238 3478 Fragments arranged by size: 3478 2275 238 199 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6190 PvuII CAG'CTG Cuts at: 2654 2680 3109 3154 3226 2654 Size: 26 429 45 72 5618 Fragments arranged by size: 5618 429 72 45 26 SacII CC_GC'GG Cuts at: 2055 3294 2055 Size: 1239 4951 SalI G'TCGA_C Cuts at: 1936 3532 1936 Size: 1596 4594 SapI GCTCTTCn'nnn_ Cuts at: 2960 3419 2960 Size: 459 5731 SbfI CC_TGCA'GG Cuts at: 3542 3542 Size: 6190 ScaI AGT'ACT Cuts at: 4438 6150 4438 Size: 1712 4478 SexAI A'CCwGG_T Cuts at: 2742 2742 Size: 6190 SfcI C'TryA_G Cuts at: 826 1045 3101 3300 3538 4673 5354 5545 826 Size: 219 2056 199 238 1135 681 191 1471 Fragments arranged by size: 2056 1471 1135 681 238 219 199 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2064 3188 2064 Size: 1124 5066 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6190 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6190 SmaI CCC'GGG Cuts at: 1872 3517 1872 Size: 1645 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2117 4294 5195 5457 820 Size: 197 926 174 2177 901 262 1553 Fragments arranged by size: 2177 1553 926 901 262 197 174 SnaBI TAC'GTA Cuts at: 493 3981 493 Size: 3488 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6190 SphI G_CATG'C Cuts at: 1132 3548 1132 Size: 2416 3774 SspI AAT'ATT Cuts at: 5 52 4114 5 Size: 47 4062 2081 Fragments arranged by size: 4062 2081 47 StuI AGG'CCT Cuts at: 1517 2408 1517 Size: 891 5299 StyI C'CwwG_G Cuts at: 513 1065 2004 2601 2859 3629 513 Size: 552 939 597 258 770 3074 Fragments arranged by size: 3074 939 770 597 552 258 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2503 2548 2729 2834 4217 4234 4387 Size: 1255 923 45 181 105 1383 17 153 Cuts at: 4387 4572 5017 325 Size: 185 445 1498 Fragments arranged by size: 1498 1383 1255 923 445 185 181 153 105 45 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4436 6148 96 Size: 274 80 33 51 1443 2459 1712 138 Fragments arranged by size: 2459 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6150 6182 6150 Size: 32 6158 Tth111I GACn'n_nGTC Cuts at: 1316 2744 1316 Size: 1428 4762 XbaI T'CTAG_A Cuts at: 3526 3526 Size: 6190 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2291 2422 2530 2291 Size: 131 108 5951 Fragments arranged by size: 5951 131 108 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6190 XmaI C'CCGG_G Cuts at: 1870 3515 1870 Size: 1645 4545 XmnI GAAnn'nnTTC Cuts at: 4319 4319 Size: 6190 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2098 3998 276 Size: 53 83 186 936 564 1900 2468 Fragments arranged by size: 2468 1900 936 564 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SfoI SrfI SwaI