(Linear) (Six-base) MAPSORT of: KIEE3602.seq Check: 8303 from: 1 to: 1485 KIEE3602.orf With 182 enzymes: SgfI * March 22, 2012 16:37 .. AatII G_ACGT'C Cuts at: 0 95 1485 Size: 95 1390 AccI GT'mk_AC Cuts at: 0 704 1485 Size: 704 781 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 295 936 1365 1485 Size: 295 641 429 120 Fragments arranged by size: 641 429 295 120 AflIII A'CryG_T Cuts at: 0 149 1485 Size: 149 1336 AhdI GACnn_n'nnGTC Cuts at: 0 641 1485 Size: 641 844 AleI CACnn'nnGTG Cuts at: 0 817 1297 1485 Size: 817 480 188 Fragments arranged by size: 817 480 188 AlwNI CAG_nnn'CTG Cuts at: 0 221 753 1015 1104 1221 1224 1388 1485 Size: 221 532 262 89 117 3 164 97 Fragments arranged by size: 532 262 221 164 117 97 89 3 ApaI G_GGCC'C Cuts at: 0 1179 1485 Size: 1179 306 ApaLI G'TGCA_C Cuts at: 0 467 1456 1485 Size: 467 989 29 Fragments arranged by size: 989 467 29 AvaI C'yCGr_G Cuts at: 0 480 826 1157 1485 Size: 480 346 331 328 Fragments arranged by size: 480 346 331 328 BanI G'GyrC_C Cuts at: 0 610 1161 1485 Size: 610 551 324 Fragments arranged by size: 610 551 324 BanII G_rGCy'C Cuts at: 0 950 1146 1179 1485 Size: 950 196 33 306 Fragments arranged by size: 950 306 196 33 BbsI GAAGACnn'nnnn_ Cuts at: 0 990 1485 Size: 990 495 BbvCI CC'TCA_GC Cuts at: 0 650 1485 Size: 650 835 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 396 430 1062 1096 1485 Size: 396 34 632 34 389 Fragments arranged by size: 632 396 389 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 59 1183 1485 Size: 59 1124 302 Fragments arranged by size: 1124 302 59 BglII A'GATC_T Cuts at: 0 933 1485 Size: 933 552 BlpI GC'TnA_GC Cuts at: 0 1145 1485 Size: 1145 340 Bme1580I G_kGCm'C Cuts at: 0 323 343 471 768 1179 1375 1460 1485 Size: 323 20 128 297 411 196 85 25 Fragments arranged by size: 411 323 297 196 128 85 25 20 BmgBI CAC'GTC Cuts at: 0 152 1485 Size: 152 1333 BmrI ACTGGGnnnn_n' Cuts at: 0 438 598 1003 1485 Size: 438 160 405 482 Fragments arranged by size: 482 438 405 160 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 231 550 730 754 1485 Size: 231 319 180 24 731 Fragments arranged by size: 731 319 231 180 24 Bpu10I CC'TnA_GC Cuts at: 0 650 1485 Size: 650 835 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 97 1485 Size: 97 1388 BsaHI Gr'CG_yC Cuts at: 0 92 1485 Size: 92 1393 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1369 1399 1485 Size: 1369 30 86 Fragments arranged by size: 1369 86 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 951 1485 Size: 951 534 BseYI C'CCAG_C Cuts at: 0 506 551 860 1015 1304 1485 Size: 506 45 309 155 289 181 Fragments arranged by size: 506 309 289 181 155 45 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1234 1485 Size: 1234 251 BsiHKAI G_wGCw'C Cuts at: 0 419 471 1460 1485 Size: 419 52 989 25 Fragments arranged by size: 989 419 52 25 Bsp1286I G_dGCh'C Cuts at: 0 323 343 419 471 768 950 1146 1179 Size: 323 20 76 52 297 182 196 33 Cuts at: 1179 1375 1460 1485 Size: 196 85 25 Fragments arranged by size: 323 297 196 196 182 85 76 52 33 25 20 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 533 1485 Size: 533 952 BsrFI r'CCGG_y Cuts at: 0 58 174 560 1485 Size: 58 116 386 925 Fragments arranged by size: 925 386 116 58 BssHII G'CGCG_C Cuts at: 0 1251 1485 Size: 1251 234 BstAPI GCAn_nnn'nTGC Cuts at: 0 1221 1485 Size: 1221 264 BstXI CCAn_nnnn'nTGG Cuts at: 0 603 1485 Size: 603 882 BstYI r'GATC_y Cuts at: 0 933 1485 Size: 933 552 BtgI C'CryG_G Cuts at: 0 47 574 596 777 854 1085 1286 1485 Size: 47 527 22 181 77 231 201 199 Fragments arranged by size: 527 231 201 199 181 77 47 22 BtsI GCAGTG_nn' Cuts at: 0 19 431 918 1124 1292 1304 1485 Size: 19 412 487 206 168 12 181 Fragments arranged by size: 487 412 206 181 168 19 12 EaeI y'GGCC_r Cuts at: 0 2 41 172 571 687 729 758 796 Size: 2 39 131 399 116 42 29 38 Cuts at: 796 1088 1184 1485 Size: 292 96 301 Fragments arranged by size: 399 301 292 131 116 96 42 39 38 29 2 EarI CTCTTCn'nnn_ Cuts at: 0 485 955 1414 1485 Size: 485 470 459 71 Fragments arranged by size: 485 470 459 71 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1452 1485 Size: 1452 33 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 231 295 550 730 754 936 1365 1485 Size: 231 64 255 180 24 182 429 120 Fragments arranged by size: 429 255 231 182 180 120 64 24 EcoNI CCTnn'n_nnAGG Cuts at: 0 301 1485 Size: 301 1184 EcoO109I rG'GnC_Cy Cuts at: 0 741 1485 Size: 741 744 FspI TGC'GCA Cuts at: 0 1366 1485 Size: 1366 119 HaeII r_GCGC'y Cuts at: 0 310 1312 1485 Size: 310 1002 173 Fragments arranged by size: 1002 310 173 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 627 659 1128 1160 1369 1401 1485 Size: 627 32 469 32 209 32 84 Fragments arranged by size: 627 469 209 84 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 270 1485 Size: 270 1215 MscI TGG'CCA Cuts at: 0 760 1090 1485 Size: 760 330 395 Fragments arranged by size: 760 395 330 MslI CAynn'nnrTG Cuts at: 0 154 424 817 1210 1297 1485 Size: 154 270 393 393 87 188 Fragments arranged by size: 393 393 270 188 154 87 MspA1I CmG'CkG Cuts at: 0 33 49 367 649 675 864 1104 1149 Size: 33 16 318 282 26 189 240 45 Cuts at: 1149 1221 1288 1485 Size: 72 67 197 Fragments arranged by size: 318 282 240 197 189 72 67 45 33 26 16 NaeI GCC'GGC Cuts at: 0 60 176 1485 Size: 60 116 1309 Fragments arranged by size: 1309 116 60 NcoI C'CATG_G Cuts at: 0 596 854 1485 Size: 596 258 631 Fragments arranged by size: 631 596 258 NgoMIV G'CCGG_C Cuts at: 0 58 174 1485 Size: 58 116 1311 Fragments arranged by size: 1311 116 58 NspI r_CATG'y Cuts at: 0 1323 1485 Size: 1323 162 PflMI CCAn_nnn'nTGG Cuts at: 0 1015 1485 Size: 1015 470 PfoI T'CCnGG_A Cuts at: 0 95 128 1485 Size: 95 33 1357 Fragments arranged by size: 1357 95 33 PpuMI rG'GwC_Cy Cuts at: 0 741 1485 Size: 741 744 PspOMI G'GGCC_C Cuts at: 0 1175 1485 Size: 1175 310 PstI C_TGCA'G Cuts at: 0 1100 1299 1485 Size: 1100 199 186 Fragments arranged by size: 1100 199 186 PvuII CAG'CTG Cuts at: 0 649 675 1104 1149 1221 1485 Size: 649 26 429 45 72 264 Fragments arranged by size: 649 429 264 72 45 26 SacII CC_GC'GG Cuts at: 0 50 1289 1485 Size: 50 1239 196 Fragments arranged by size: 1239 196 50 SapI GCTCTTCn'nnn_ Cuts at: 0 955 1414 1485 Size: 955 459 71 Fragments arranged by size: 955 459 71 SexAI A'CCwGG_T Cuts at: 0 737 1485 Size: 737 748 SfcI C'TryA_G Cuts at: 0 1096 1295 1485 Size: 1096 199 190 Fragments arranged by size: 1096 199 190 SfiI GGCCn_nnn'nGGCC Cuts at: 0 59 1183 1485 Size: 59 1124 302 Fragments arranged by size: 1124 302 59 SmlI C'TyrA_G Cuts at: 0 112 1485 Size: 112 1373 StuI AGG'CCT Cuts at: 0 403 1485 Size: 403 1082 StyI C'CwwG_G Cuts at: 0 596 854 1485 Size: 596 258 631 Fragments arranged by size: 631 596 258 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 498 543 724 829 1485 Size: 498 45 181 105 656 Fragments arranged by size: 656 498 181 105 45 Tth111I GACn'n_nGTC Cuts at: 0 739 1485 Size: 739 746 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 286 417 525 1485 Size: 286 131 108 960 Fragments arranged by size: 960 286 131 108 ZraI GAC'GTC Cuts at: 0 93 1485 Size: 93 1392 Enzymes that do cut: AatII AccI AcuI AflIII AhdI AleI AlwNI ApaI ApaLI AvaI BanI BanII BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI BssHII BstAPI BstXI BstYI BtgI BtsI EaeI EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PflMI PfoI PpuMI PspOMI PstI PvuII SacII SapI SexAI SfcI SfiI SmlI StuI StyI TaqII Tth111I XcmI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AgeI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BclI BfrBI BmtI BplI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SbfI ScaI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI XbaI XhoI XmaI XmnI