Restriction map for
pFN21AB3659
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspHI AviII AvrII BalI BbeI
BbrPI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BspDI BspEI BspLU11I BsrBI BsrDI
BssHII Bst1107I BstAPI BstBI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FspI HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MseI MunI NarI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tru9I Tsp509I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccII 330 CGCG CG^CG
AcyI 490 GACGCC G[GA]^CG[CT]C
AhdI 210 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 210 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 298 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 448 CTCGGG C^[CT]CG[GA]G
BamHI 850 GGATCC G^GATCC
BanI 187 GGCACC G^G[CT][GA]CC
BbsI 723 GAAGAC GAAGAC(2/6)
BbvCI 583 GCTGAGG CCTCAGC(-5/-2)
BcgI 440 GCATACACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 723 GAAGAC GAAGAC(2/6)
BsaHI 490 GACGCC G[GA]^CG[CT]C
BsmAI 664 GTCTC GTCTC(1/5)
BsmI 564 GAATGC GAATGC(1/-1)
BsoBI 448 CTCGGG C^[CT]CG[GA]G
Bsp1407I 314 TGTACA T^GTACA
BspHI 557 TCATGA T^CATGA
BsrGI 314 TGTACA T^GTACA
BssSI 163 CTCGTG CACGAG(-5/-1)
BstUI 330 CGCG CG^CG
Eam1105I 210 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 479 GCGGG CCCGC(4/6)
FseI 246 GGCCGGCC GGCCGG^CC
HaeII 257 AGCGCC [GA]GCGC^[CT]
HgaI 490 GACGC GACGC(5/10)
Hin1I 490 GACGCC G[GA]^CG[CT]C
HincII 574 GTCAAC GT[CT]^[GA]AC
HindII 574 GTCAAC GT[CT]^[GA]AC
MboII 723 GAAGA GAAGA(8/7)
MvnI 330 CGCG CG^CG
PflFI 298 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 216 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 886 CAGCTG CAG^CTG
RcaI 557 TCATGA T^CATGA
ScaI 755 AGTACT AGT^ACT
SfaNI 284 GCATC GCATC(5/9)
SmlI 607 CTCAAG C^T[CT][GA]AG
SspBI 314 TGTACA T^GTACA
TaqI 770 TCGA T^CGA
TfiI 925 GAATC G^A[AT]TC
Tth111I 298 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 770 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
ApaI 801 GGGCCC GGGCC^C
896 GGGCCC
BanII 801 GGGCCC G[GA]GC[CT]^C
896 GGGCCC
BpmI 339 CTCCAG CTGGAG(16/14)
746 CTCCAG
BsgI 369 CTGCAC GTGCAG(16/14)
166 GTGCAG
BsmFI 481 GGGAC GGGAC(10/14)
218 GTCCC
BspMI 632 ACCTGC ACCTGC(4/8)
309 GCAGGT
BsrI 99 ACTGG ACTGG(1/-1)
753 CCAGT
BstEII 390 GGTGACC G^GT[ACGT]ACC
591 GGTGACC
BstPI 390 GGTGACC G^GT[ACGT]ACC
591 GGTGACC
BstXI 407 CCACAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
692 CCACAGTGGTGG
BstYI 125 AGATCC [GA]^GATC[CT]
850 GGATCC
CfoI 258 GCGC GCG^C
329 GCGC
DdeI 212 CTGAG C^T[ACGT]AG
584 CTGAG
DpnII 126 GATC ^GATC
851 GATC
EaeI 245 TGGCCG [CT]^GGCC[GA]
362 TGGCCG
EcoO65I 390 GGTGACC G^GT[ACGT]ACC
591 GGTGACC
HhaI 258 GCGC GCG^C
329 GCGC
HinP1I 258 GCGC G^CGC
329 GCGC
MboI 126 GATC ^GATC
851 GATC
MflI 125 AGATCC [GA]^GATC[CT]
850 GGATCC
MspA1I 477 CAGCGG C[AC]G^C[GT]G
886 CAGCTG
NdeII 126 GATC ^GATC
851 GATC
PleI 298 GACTC GAGTC(4/5)
521 GAGTC
Sau3AI 126 GATC ^GATC
851 GATC
Tsp45I 391 GTGAC ^GT[GC]AC
592 GTGAC
XcmI 191 CCATCCTGACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
374 CCACAGGAGCCATGG
XhoII 125 AGATCC [GA]^GATC[CT]
850 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BcnI BmyI BsaJI BsiYI
BslI Bsp1286I BsrFI BssKI BstF5I
BstNI Cac8I Cfr10I Cfr13I DraII
DsaI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeII MaeIII MnlI
MslI MspI MvaI MwoI NaeI
NciI NcoI NgoMIV NlaIII NlaIV
PstI RsaI Sau96I ScrFI SfcI
StyI TseI TspRI