Restriction map for
pFN21AB3659
(Circular) (Six-base) MAPSORT of: pFN21AB3659.seq Check: 280 from: 1 to: 5641
pFN21AB3659 5641 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2305 2646 2305
Size: 341 5300
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3451 278
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2984 1937
Size: 1047 4594
Acc65I G'GTAC_C
Cuts at: 2962 2962
Size: 5641
AclI AA'CG_TT
Cuts at: 1563 3768 4141 1563
Size: 2205 373 3063
Fragments arranged by size:
3063 2205 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2007 2036 2749 3704 5088 5356 1632
Size: 375 29 713 955 1384 268 1917
Fragments arranged by size:
1917 1384 955 713 375 268 29
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5444
AflIII A'CryG_T
Cuts at: 1203 4540 5517 1203
Size: 3337 977 1327
Fragments arranged by size:
3337 1327 977
AgeI A'CCGG_T
Cuts at: 4472 4472
Size: 5641
AhdI GACnn_n'nnGTC
Cuts at: 2220 4370 2220
Size: 2150 3491
AleI CACnn'nnGTG
Cuts at: 1166 2417 2702 1166
Size: 1251 285 4105
Fragments arranged by size:
4105 1251 285
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5641
AlwNI CAG_nnn'CTG
Cuts at: 3384 4956 3384
Size: 1572 4069
ApaI G_GGCC'C
Cuts at: 2810 2905 2810
Size: 95 5546
ApaLI G'TGCA_C
Cuts at: 3698 4854 3698
Size: 1156 4485
ApoI r'AATT_y
Cuts at: 1493 2950 3176 3328 5437 1493
Size: 1457 226 152 2109 1697
Fragments arranged by size:
2109 1697 1457 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4195 160
Size: 872 3163 1606
Fragments arranged by size:
3163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
AvaI C'yCGr_G
Cuts at: 1870 1943 2453 2966 1870
Size: 73 510 513 4545
Fragments arranged by size:
4545 513 510 73
BamHI G'GATC_C
Cuts at: 2855 2971 3338 2855
Size: 116 367 5158
Fragments arranged by size:
5158 367 116
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2192 2962 4417 618
Size: 325 217 627 405 770 1455 1842
Fragments arranged by size:
1842 1455 770 627 405 325 217
BanII G_rGCy'C
Cuts at: 1391 2810 2905 2960 1391
Size: 1419 95 55 4072
Fragments arranged by size:
4072 1419 95 55
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2735 928
Size: 971 836 3834
Fragments arranged by size:
3834 971 836
BbvCI CC'TCA_GC
Cuts at: 2589 2589
Size: 5641
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2434 2468 3832 3866 1903
Size: 34 497 34 1364 34 3678
Fragments arranged by size:
3678 1364 497 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3533 4743 3533
Size: 1210 4431
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5641
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4252 136
Size: 107 122 71 631 605 2580 1525
Fragments arranged by size:
2580 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5636 5636
Size: 5641
BlpI GC'TnA_GC
Cuts at: 3058 3058
Size: 5641
Bme1580I G_kGCm'C
Cuts at: 1790 2195 2407 2810 2905 3702 4858 1790
Size: 405 212 403 95 797 1156 2573
Fragments arranged by size:
2573 1156 797 405 403 212 95
BmgBI CAC'GTC
Cuts at: 1318 2029 2722 1318
Size: 711 693 4237
Fragments arranged by size:
4237 711 693
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3372 4330 442
Size: 416 53 83 399 24 1955 958 1753
Fragments arranged by size:
1955 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2329 2736 4301 1239
Size: 1090 407 1565 2579
Fragments arranged by size:
2579 1565 1090 407
Bpu10I CC'TnA_GC
Cuts at: 2589 2589
Size: 5641
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2597 3766 4631 4929 2597
Size: 1169 865 298 3309
Fragments arranged by size:
3309 1169 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4304 882
Size: 617 2805 2219
Fragments arranged by size:
2805 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3432 493
Size: 711 2228 2702
Fragments arranged by size:
2702 2228 711
BsaBI GATnn'nnATC
Cuts at: 5635 5635
Size: 5641
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2496 3448 3830 4465
Size: 53 83 186 936 963 952 382 635
Cuts at: 4465 275
Size: 1451
Fragments arranged by size:
1451 963 952 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4074 4472 4746 4893 5304 4074
Size: 398 274 147 411 4411
Fragments arranged by size:
4411 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5416
Fragments arranged by size:
5416 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5641
BseYI C'CCAG_C
Cuts at: 2188 2406 4844 5340 2188
Size: 218 2438 496 2489
Fragments arranged by size:
2489 2438 496 218
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2192 2359 1163
Size: 573 456 167 4445
Fragments arranged by size:
4445 573 456 167
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3091 3852 4880 1540
Size: 275 186 1090 761 1028 2301
Fragments arranged by size:
2301 1090 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3702 3787 4858 3702
Size: 85 1071 4485
Fragments arranged by size:
4485 1071 85
BsmI GAATG_Cn'
Cuts at: 2575 3152 3245 2575
Size: 577 93 4971
Fragments arranged by size:
4971 577 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2195 2407 2810 2905 2960 3702 3787
Size: 399 405 212 403 95 55 742 85
Cuts at: 3787 4858 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 405 403 399 212 95
85 55
BspEI T'CCGG_A
Cuts at: 5304 5304
Size: 5641
BspHI T'CATG_A
Cuts at: 2562 3530 5260 2562
Size: 968 1730 2943
Fragments arranged by size:
2943 1730 968
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2305 2646 2996 844
Size: 1461 341 350 3489
Fragments arranged by size:
3489 1461 350 341
BsrBI CCG'CTC
Cuts at: 1394 1823 3528 1394
Size: 429 1705 3507
Fragments arranged by size:
3507 1705 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4136 4310 65
Size: 1183 2888 174 1396
Fragments arranged by size:
2888 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2252 2369 2684 4285 4472 1700
Size: 110 442 117 315 1601 187 2869
Fragments arranged by size:
2869 1601 442 315 187 117 110
BsrGI T'GTAC_A
Cuts at: 96 2319 96
Size: 2223 3418
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5641
BssSI C'ACGA_G
Cuts at: 2168 3701 4713 2168
Size: 1533 1012 3096
Fragments arranged by size:
3096 1533 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5641
BstBI TT'CG_AA
Cuts at: 4445 4445
Size: 5641
BstEII G'GTnAC_C
Cuts at: 1184 2395 2596 1184
Size: 1211 201 4229
Fragments arranged by size:
4229 1211 201
BstXI CCAn_nnnn'nTGG
Cuts at: 2419 2704 2419
Size: 285 5356
BstYI r'GATC_y
Cuts at: 1972 2130 2855 2971 3338 3723 3740 5192 5278
Size: 158 725 116 367 385 17 1452 86
Cuts at: 5278 5290 5636 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 725 385 367 346 158 116
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2040 2179 2205 2388 513
Size: 552 939 36 139 26 183 3766
Fragments arranged by size:
3766 939 552 183 139 36 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2494 487
Size: 683 56 1268 3634
Fragments arranged by size:
3634 1268 683 56
BtsI GCAGTG_nn'
Cuts at: 3157 3951 3971 3157
Size: 794 20 4827
Fragments arranged by size:
4827 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5606
DraI TTT'AAA
Cuts at: 2945 2945
Size: 5641
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4648 809
Size: 507 3332 1802
Fragments arranged by size:
3332 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2250 2367 3088
Size: 54 1425 50 275 18 420 117 721
Cuts at: 3088 3977 5606 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 721 420 275 117 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3088 1537
Size: 275 1276 4090
Fragments arranged by size:
4090 1276 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3571 1353
Size: 2218 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2111 4228 4602 4748 387
Size: 1064 138 522 2117 374 146 1280
Fragments arranged by size:
2117 1280 1064 522 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2007 2036 2329 2736 2749 3704 4301
Size: 393 375 29 293 407 13 955 597
Cuts at: 4301 5088 5356 1239
Size: 787 268 1524
Fragments arranged by size:
1524 955 787 597 407 393 375 293
268 29 13
EcoO109I rG'GnC_Cy
Cuts at: 2222 2533 2806 2807 2902 2222
Size: 311 273 1 95 4961
Fragments arranged by size:
4961 311 273 95 1
EcoRI G'AATT_C
Cuts at: 2950 3328 2950
Size: 378 5263
FseI GG_CCGG'CC
Cuts at: 2256 2256
Size: 5641
FspI TGC'GCA
Cuts at: 4147 4147
Size: 5641
HaeII r_GCGC'y
Cuts at: 1678 2266 4788 1678
Size: 588 2522 2531
Fragments arranged by size:
2531 2522 588
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2206 2238 4282 4314 4356
Size: 32 135 32 634 32 2044 32 42
Cuts at: 4356 4388 1373
Size: 32 2626
Fragments arranged by size:
2626 2044 634 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2581 2985 3231 669
Size: 988 281 643 404 246 3079
Fragments arranged by size:
3079 988 643 404 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5641
HpaI GTT'AAC
Cuts at: 3231 3231
Size: 5641
KpnI G_GTAC'C
Cuts at: 2966 2966
Size: 5641
MfeI C'AATT_G
Cuts at: 3240 3240
Size: 5641
MluI A'CGCG_T
Cuts at: 5517 5517
Size: 5641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1990 4755 4939 1305
Size: 685 2765 184 2007
Fragments arranged by size:
2765 2007 685 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3819
Fragments arranged by size:
3819 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2045 2393 2417 2702 3599 3958 4117
Size: 648 879 348 24 285 897 359 159
Cuts at: 4117 518
Size: 2042
Fragments arranged by size:
2042 897 879 648 359 348 285 159
24
MspA1I CmG'CkG
Cuts at: 1606 2484 2893 3057 3734 4882 5127 1606
Size: 878 409 164 677 1148 245 2120
Fragments arranged by size:
2120 1148 878 677 409 245 164
NaeI GCC'GGC
Cuts at: 2254 2371 2686 2254
Size: 117 315 5209
Fragments arranged by size:
5209 315 117
NcoI C'CATG_G
Cuts at: 513 1065 2004 2040 2205 2388 513
Size: 552 939 36 165 183 3766
Fragments arranged by size:
3766 939 552 183 165 36
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5641
NgoMIV G'CCGG_C
Cuts at: 2252 2369 2684 2252
Size: 117 315 5209
Fragments arranged by size:
5209 315 117
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5641
NotI GC'GGCC_GC
Cuts at: 3088 3088
Size: 5641
NspI r_CATG'y
Cuts at: 1132 1229 2999 4544 1132
Size: 97 1770 1545 2229
Fragments arranged by size:
2229 1770 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5641
PciI A'CATG_T
Cuts at: 4540 4540
Size: 5641
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5641
PfoI T'CCnGG_A
Cuts at: 2462 2858 2462
Size: 396 5245
PmeI GTTT'AAAC
Cuts at: 2945 2945
Size: 5641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 5223 5255 3710
Size: 32 1481 32 4096
Fragments arranged by size:
4096 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2222 2222
Size: 5641
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5641
PsiI TTA'TAA
Cuts at: 3211 3211
Size: 5641
PspOMI G'GGCC_C
Cuts at: 2806 2901 2806
Size: 95 5546
PstI C_TGCA'G
Cuts at: 830 2130 2231 2262 2993 830
Size: 1300 101 31 731 3478
Fragments arranged by size:
3478 1300 731 101 31
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5641
PvuII CAG'CTG
Cuts at: 2893 2893
Size: 5641
SalI G'TCGA_C
Cuts at: 1936 2983 1936
Size: 1047 4594
SanDI GG'GwC_CC
Cuts at: 2222 2222
Size: 5641
SbfI CC_TGCA'GG
Cuts at: 2993 2993
Size: 5641
ScaI AGT'ACT
Cuts at: 2762 3889 5601 2762
Size: 1127 1712 2802
Fragments arranged by size:
2802 1712 1127
SfcI C'TryA_G
Cuts at: 826 1045 2126 2227 2258 2500 2989 4124 4805
Size: 219 1081 101 31 242 489 1135 681
Cuts at: 4805 4996 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1081 681 489 242 219 191
101 31
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5641
SmaI CCC'GGG
Cuts at: 1872 2968 1872
Size: 1096 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2612 3745 4646 4908 820
Size: 197 926 669 1133 901 262 1553
Fragments arranged by size:
1553 1133 926 901 669 262 197
SnaBI TAC'GTA
Cuts at: 493 3432 493
Size: 2939 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5641
SphI G_CATG'C
Cuts at: 1132 2999 1132
Size: 1867 3774
SspI AAT'ATT
Cuts at: 5 52 3565 5
Size: 47 3513 2081
Fragments arranged by size:
3513 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5641
StyI C'CwwG_G
Cuts at: 513 1065 2004 2040 2115 2205 2388 3080 513
Size: 552 939 36 75 90 183 692 3074
Fragments arranged by size:
3074 939 692 552 183 90 75 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3668 3685 3838 4023 4468 325
Size: 1255 2088 17 153 185 445 1498
Fragments arranged by size:
2088 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2319 2760 3887
Size: 274 80 33 51 1443 342 441 1127
Cuts at: 3887 5599 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1127 441 342 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5601 5633 5601
Size: 32 5609
Tth111I GACn'n_nGTC
Cuts at: 1316 2306 1316
Size: 990 4651
XbaI T'CTAG_A
Cuts at: 2977 2977
Size: 5641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2203 2386 2203
Size: 183 5458
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5641
XmaI C'CCGG_G
Cuts at: 1870 2966 1870
Size: 1096 4545
XmnI GAAnn'nnTTC
Cuts at: 3770 3770
Size: 5641
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3449 276
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FspAI KasI NarI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacI SacII SapI SexAI SfiI
SfoI SrfI SwaI