Restriction map for
pFN21AB3659
(Linear) (Six-base) MAPSORT of: KIBB3659.seq Check: 1107 from: 1 to: 936
KIBB3659.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 300 641 936
Size: 300 341 295
Fragments arranged by size:
341 300 295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 31 744 936
Size: 2 29 713 192
Fragments arranged by size:
713 192 29 2
AhdI GACnn_n'nnGTC
Cuts at: 0 215 936
Size: 215 721
AleI CACnn'nnGTG
Cuts at: 0 412 697 936
Size: 412 285 239
Fragments arranged by size:
412 285 239
ApaI G_GGCC'C
Cuts at: 0 805 900 936
Size: 805 95 36
Fragments arranged by size:
805 95 36
AvaI C'yCGr_G
Cuts at: 0 448 936
Size: 448 488
BamHI G'GATC_C
Cuts at: 0 850 936
Size: 850 86
BanI G'GyrC_C
Cuts at: 0 187 936
Size: 187 749
BanII G_rGCy'C
Cuts at: 0 805 900 936
Size: 805 95 36
Fragments arranged by size:
805 95 36
BbsI GAAGACnn'nnnn_
Cuts at: 0 730 936
Size: 730 206
BbvCI CC'TCA_GC
Cuts at: 0 584 936
Size: 584 352
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 429 463 936
Size: 429 34 473
Fragments arranged by size:
473 429 34
Bme1580I G_kGCm'C
Cuts at: 0 190 402 805 900 936
Size: 190 212 403 95 36
Fragments arranged by size:
403 212 190 95 36
BmgBI CAC'GTC
Cuts at: 0 24 717 936
Size: 24 693 219
Fragments arranged by size:
693 219 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 731 936
Size: 324 407 205
Fragments arranged by size:
407 324 205
Bpu10I CC'TnA_GC
Cuts at: 0 584 936
Size: 584 352
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 936
Size: 592 344
BsaHI Gr'CG_yC
Cuts at: 0 491 936
Size: 491 445
BseYI C'CCAG_C
Cuts at: 0 183 401 936
Size: 183 218 535
Fragments arranged by size:
535 218 183
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 354 936
Size: 187 167 582
Fragments arranged by size:
582 187 167
BsmI GAATG_Cn'
Cuts at: 0 570 936
Size: 570 366
Bsp1286I G_dGCh'C
Cuts at: 0 190 402 805 900 936
Size: 190 212 403 95 36
Fragments arranged by size:
403 212 190 95 36
BspHI T'CATG_A
Cuts at: 0 557 936
Size: 557 379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 300 641 936
Size: 300 341 295
Fragments arranged by size:
341 300 295
BsrFI r'CCGG_y
Cuts at: 0 247 364 679 936
Size: 247 117 315 257
Fragments arranged by size:
315 257 247 117
BsrGI T'GTAC_A
Cuts at: 0 314 936
Size: 314 622
BssSI C'ACGA_G
Cuts at: 0 163 936
Size: 163 773
BstEII G'GTnAC_C
Cuts at: 0 390 591 936
Size: 390 201 345
Fragments arranged by size:
390 345 201
BstXI CCAn_nnnn'nTGG
Cuts at: 0 414 699 936
Size: 414 285 237
Fragments arranged by size:
414 285 237
BstYI r'GATC_y
Cuts at: 0 125 850 936
Size: 125 725 86
Fragments arranged by size:
725 125 86
BtgI C'CryG_G
Cuts at: 0 35 174 200 383 936
Size: 35 139 26 183 553
Fragments arranged by size:
553 183 139 35 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 489 936
Size: 489 447
EaeI y'GGCC_r
Cuts at: 0 245 362 936
Size: 245 117 574
Fragments arranged by size:
574 245 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 106 936
Size: 106 830
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 31 324 731 744 936
Size: 2 29 293 407 13 192
Fragments arranged by size:
407 293 192 29 13 2
EcoO109I rG'GnC_Cy
Cuts at: 0 217 528 801 802 897 936
Size: 217 311 273 1 95 39
Fragments arranged by size:
311 273 217 95 39 1
FseI GG_CCGG'CC
Cuts at: 0 251 936
Size: 251 685
HaeII r_GCGC'y
Cuts at: 0 261 936
Size: 261 675
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 201 233 936
Size: 201 32 703
Fragments arranged by size:
703 201 32
HincII GTy'rAC
Cuts at: 0 576 936
Size: 576 360
MslI CAynn'nnrTG
Cuts at: 0 40 388 412 697 936
Size: 40 348 24 285 239
Fragments arranged by size:
348 285 239 40 24
MspA1I CmG'CkG
Cuts at: 0 479 888 936
Size: 479 409 48
Fragments arranged by size:
479 409 48
NaeI GCC'GGC
Cuts at: 0 249 366 681 936
Size: 249 117 315 255
Fragments arranged by size:
315 255 249 117
NcoI C'CATG_G
Cuts at: 0 35 200 383 936
Size: 35 165 183 553
Fragments arranged by size:
553 183 165 35
NgoMIV G'CCGG_C
Cuts at: 0 247 364 679 936
Size: 247 117 315 257
Fragments arranged by size:
315 257 247 117
PfoI T'CCnGG_A
Cuts at: 0 457 853 936
Size: 457 396 83
Fragments arranged by size:
457 396 83
PpuMI rG'GwC_Cy
Cuts at: 0 217 936
Size: 217 719
PspOMI G'GGCC_C
Cuts at: 0 801 896 936
Size: 801 95 40
Fragments arranged by size:
801 95 40
PstI C_TGCA'G
Cuts at: 0 125 226 257 936
Size: 125 101 31 679
Fragments arranged by size:
679 125 101 31
PvuII CAG'CTG
Cuts at: 0 888 936
Size: 888 48
SanDI GG'GwC_CC
Cuts at: 0 217 936
Size: 217 719
ScaI AGT'ACT
Cuts at: 0 757 936
Size: 757 179
SfcI C'TryA_G
Cuts at: 0 121 222 253 495 936
Size: 121 101 31 242 441
Fragments arranged by size:
441 242 121 101 31
SmlI C'TyrA_G
Cuts at: 0 607 936
Size: 607 329
StyI C'CwwG_G
Cuts at: 0 35 110 200 383 936
Size: 35 75 90 183 553
Fragments arranged by size:
553 183 90 75 35
TatI w'GTAC_w
Cuts at: 0 314 755 936
Size: 314 441 181
Fragments arranged by size:
441 314 181
Tth111I GACn'n_nGTC
Cuts at: 0 301 936
Size: 301 635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 198 381 936
Size: 198 183 555
Fragments arranged by size:
555 198 183
Enzymes that do cut:
AarI AcuI AhdI AleI ApaI AvaI BamHI BanI
BanII BbsI BbvCI BcgI Bme1580I BmgBI BpmI Bpu10I
BpuEI BsaHI BseYI BsgI BsmI Bsp1286I BspHI BspMI
BsrFI BsrGI BssSI BstEII BstXI BstYI BtgI BtgZI
EaeI EciI Eco57MI EcoO109I FseI HaeII Hin4I HincII
MslI MspA1I NaeI NcoI NgoMIV PfoI PpuMI PspOMI
PstI PvuII SanDI ScaI SfcI SmlI StyI TatI
Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BciVI BclI BfrBI BglI BglII
BlpI BmrI BmtI BplI BsaI BsaAI BsaBI BsaWI
BsaXI BseRI BsiEI BsiHKAI BsiWI BsmBI BspEI BsrBI
BsrDI BssHII BstAPI BstBI BstZ17I Bsu36I BtsI ClaI
CspCI DraI DraIII DrdI EagI EarI EcoICRI EcoNI
EcoRI EcoRV FalI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI NarI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TstI XbaI XhoI XmaI
XmnI ZraI