Restriction map for
pFN21AB3655
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII BanI BbeI BbrPI BbvCI
BclI BfrI BglI BsaAI BsaBI
BsaHI BsaI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspHI BsrBI
BsrFI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI Tth111I Van91I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcsI 536 GAATTC [GA]^AATT[CT]
AflIII 803 ACATGT A^C[GA][CT]GT
AlwNI 293 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 172 CCTGAGG CC^T[ACGT]AGG
ApoI 536 GAATTC [GA]^AATT[CT]
Asp700I 1169 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1152 GAGCTC G[AT]GC[AT]^C
AvaI 1229 CCCGAG C^[CT]CG[GA]G
AvrII 1503 CCTAGG C^CTAGG
BanII 1152 GAGCTC G[GA]GC[CT]^C
BbsI 1282 GTCTTC GAAGAC(2/6)
BcgI 858 GCAGGAAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 420 GTATCC GTATCC(6/5)
BcnI 1497 CCGGG CC^[GC]GG
BglII 161 AGATCT A^GATCT
BlnI 1503 CCTAGG C^CTAGG
BlpI 500 GCTCAGC GC^T[ACGT]AGC
BmyI 1152 GAGCTC G[AGT]GC[ACT]^C
Bpu1102I 500 GCTCAGC GC^T[ACGT]AGC
BpuAI 1282 GTCTTC GAAGAC(2/6)
BsgI 1109 CTGCAC GTGCAG(16/14)
BsiHKAI 1152 GAGCTC G[AT]GC[AT]^C
BsmAI 943 GTCTC GTCTC(1/5)
BsmI 813 GCATTC GAATGC(1/-1)
BsoBI 1229 CCCGAG C^[CT]CG[GA]G
Bsp1286I 1152 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 1000 TGTACA T^GTACA
BspLU11I 803 ACATGT A^CATGT
BsrGI 1000 TGTACA T^GTACA
BssSI 434 CACGAG CACGAG(-5/-1)
BstXI 332 CCACCCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 172 CCTGAGG CC^T[ACGT]AGG
CelII 500 GCTCAGC GC^T[ACGT]AGC
DsaI 1668 CCGTGG C^C[GA][CT]GG
EarI 589 CTCTTC CTCTTC(1/4)
Eco81I 172 CCTGAGG CC^T[ACGT]AGG
EcoRI 536 GAATTC G^AATTC
Ksp632I 589 CTCTTC CTCTTC(1/4)
MaeII 1676 ACGT A^CGT
MspA1I 355 CAGCGG C[AC]G^C[GT]G
NciI 1497 CCGGG CC^[GC]GG
PstI 1142 CTGCAG CTGCA^G
SacI 1152 GAGCTC GAGCT^C
SexAI 225 ACCAGGT A^CC[AT]GGT
SmlI 709 CTCAAG C^T[CT][GA]AG
SspBI 1000 TGTACA T^GTACA
SspI 265 AATATT AAT^ATT
StuI 1644 AGGCCT AGG^CCT
Tsp509I 537 AATT ^AATT
XbaI 832 TCTAGA T^CTAGA
XcmI 977 CCAACGAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1169 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 10 enzymes) #
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BalI 329 TGGCCA TGG^CCA
1179 TGGCCA
BsaWI 220 ACCGGA [AT]^CCGG[AT]
845 ACCGGA
BspMI 809 ACCTGC ACCTGC(4/8)
984 GCAGGT
EcoT14I 425 CCAAGG C^C[AT][AT]GG
1503 CCTAGG
MluNI 329 TGGCCA TGG^CCA
1179 TGGCCA
MscI 329 TGGCCA TGG^CCA
1179 TGGCCA
MwoI 348 GCTGCTCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
351 GCTCCAGCGGC
StyI 425 CCAAGG C^C[AT][AT]GG
1503 CCTAGG
TaqI 867 TCGA T^CGA
1048 TCGA
TthHB8I 867 TCGA T^CGA
1048 TCGA
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BamHI BbvI BfaI BpmI BsaJI
BseRI BsiYI BslI BsmFI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI NdeII NlaIII NlaIV NspI
PleI PpuMI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI TfiI
Tru9I TseI Tsp45I TspRI XhoII