Restriction map for
pFN21AB3655
(Linear) (Six-base) MAPSORT of: KIBB3655.seq Check: 882 from: 1 to: 1683
KIBB3655.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 975 1683
Size: 975 708
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 422 977 1683
Size: 422 555 706
Fragments arranged by size:
706 555 422
AflIII A'CryG_T
Cuts at: 0 803 1683
Size: 803 880
AleI CACnn'nnGTG
Cuts at: 0 337 1683
Size: 337 1346
AlwNI CAG_nnn'CTG
Cuts at: 0 298 1683
Size: 298 1385
ApoI r'AATT_y
Cuts at: 0 536 1683
Size: 536 1147
AvaI C'yCGr_G
Cuts at: 0 1229 1683
Size: 1229 454
AvrII C'CTAG_G
Cuts at: 0 1503 1683
Size: 1503 180
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 566 599 1683
Size: 566 33 1084
Fragments arranged by size:
1084 566 33
BamHI G'GATC_C
Cuts at: 0 255 317 1133 1683
Size: 255 62 816 550
Fragments arranged by size:
816 550 255 62
BanII G_rGCy'C
Cuts at: 0 1156 1683
Size: 1156 527
BbsI GAAGACnn'nnnn_
Cuts at: 0 1275 1683
Size: 1275 408
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 847 881 1683
Size: 847 34 802
Fragments arranged by size:
847 802 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 431 1683
Size: 431 1252
BglII A'GATC_T
Cuts at: 0 161 1683
Size: 161 1522
BlpI GC'TnA_GC
Cuts at: 0 501 1683
Size: 501 1182
BmrI ACTGGGnnnn_n'
Cuts at: 0 382 395 1606 1683
Size: 382 13 1211 77
Fragments arranged by size:
1211 382 77 13
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 180 201 1683
Size: 180 21 1482
Fragments arranged by size:
1482 180 21
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 308 337 1683
Size: 65 243 29 1346
Fragments arranged by size:
1346 243 65 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 1683
Size: 694 989
BsaWI w'CCGG_w
Cuts at: 0 220 845 1683
Size: 220 625 838
Fragments arranged by size:
838 625 220
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 145 190 193 1683
Size: 145 45 3 1490
Fragments arranged by size:
1490 145 45 3
BseYI C'CCAG_C
Cuts at: 0 6 691 1086 1683
Size: 6 685 395 597
Fragments arranged by size:
685 597 395 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1094 1683
Size: 1094 589
BsiHKAI G_wGCw'C
Cuts at: 0 1156 1683
Size: 1156 527
BsmI GAATG_Cn'
Cuts at: 0 813 1683
Size: 813 870
Bsp1286I G_dGCh'C
Cuts at: 0 1156 1683
Size: 1156 527
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 818 975 1683
Size: 818 157 708
Fragments arranged by size:
818 708 157
BsrDI GCAATG_nn'
Cuts at: 0 242 506 566 902 1588 1683
Size: 242 264 60 336 686 95
Fragments arranged by size:
686 336 264 242 95 60
BsrGI T'GTAC_A
Cuts at: 0 1000 1683
Size: 1000 683
BssSI C'ACGA_G
Cuts at: 0 434 1683
Size: 434 1249
BstXI CCAn_nnnn'nTGG
Cuts at: 0 339 1683
Size: 339 1344
BstYI r'GATC_y
Cuts at: 0 161 255 317 1133 1683
Size: 161 94 62 816 550
Fragments arranged by size:
816 550 161 94 62
Bsu36I CC'TnA_GG
Cuts at: 0 173 1683
Size: 173 1510
BtgI C'CryG_G
Cuts at: 0 1668 1683
Size: 1668 15
BtsI GCAGTG_nn'
Cuts at: 0 299 1133 1683
Size: 299 834 550
Fragments arranged by size:
834 550 299
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1254 1289 1683
Size: 1254 35 394
Fragments arranged by size:
1254 394 35
EaeI y'GGCC_r
Cuts at: 0 329 1179 1390 1683
Size: 329 850 211 293
Fragments arranged by size:
850 329 293 211
EarI CTCTTCn'nnn_
Cuts at: 0 595 1683
Size: 595 1088
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1443 1683
Size: 1443 240
EcoICRI GAG'CTC
Cuts at: 0 1154 1683
Size: 1154 529
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 308 337 422 977 1683
Size: 65 243 29 85 555 706
Fragments arranged by size:
706 555 243 85 65 29
EcoO109I rG'GnC_Cy
Cuts at: 0 10 429 1500 1683
Size: 10 419 1071 183
Fragments arranged by size:
1071 419 183 10
EcoRI G'AATT_C
Cuts at: 0 536 1683
Size: 536 1147
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 268 300 773 805 1683
Size: 268 32 473 32 878
Fragments arranged by size:
878 473 268 32 32
MscI TGG'CCA
Cuts at: 0 331 1181 1683
Size: 331 850 502
Fragments arranged by size:
850 502 331
MslI CAynn'nnrTG
Cuts at: 0 337 649 1471 1683
Size: 337 312 822 212
Fragments arranged by size:
822 337 312 212
MspA1I CmG'CkG
Cuts at: 0 357 1683
Size: 357 1326
NspI r_CATG'y
Cuts at: 0 486 720 807 1513 1605 1683
Size: 486 234 87 706 92 78
Fragments arranged by size:
706 486 234 92 87 78
PasI CC'CwG_GG
Cuts at: 0 118 1683
Size: 118 1565
PciI A'CATG_T
Cuts at: 0 803 1683
Size: 803 880
PfoI T'CCnGG_A
Cuts at: 0 258 1496 1610 1683
Size: 258 1238 114 73
Fragments arranged by size:
1238 258 114 73
PpuMI rG'GwC_Cy
Cuts at: 0 10 429 1500 1683
Size: 10 419 1071 183
Fragments arranged by size:
1071 419 183 10
PstI C_TGCA'G
Cuts at: 0 1146 1683
Size: 1146 537
SacI G_AGCT'C
Cuts at: 0 1156 1683
Size: 1156 527
ScaI AGT'ACT
Cuts at: 0 136 184 883 1683
Size: 136 48 699 800
Fragments arranged by size:
800 699 136 48
SexAI A'CCwGG_T
Cuts at: 0 225 1683
Size: 225 1458
SfcI C'TryA_G
Cuts at: 0 90 527 1142 1333 1683
Size: 90 437 615 191 350
Fragments arranged by size:
615 437 350 191 90
SmlI C'TyrA_G
Cuts at: 0 709 1683
Size: 709 974
SspI AAT'ATT
Cuts at: 0 267 1683
Size: 267 1416
StuI AGG'CCT
Cuts at: 0 1646 1683
Size: 1646 37
StyI C'CwwG_G
Cuts at: 0 425 1503 1683
Size: 425 1078 180
Fragments arranged by size:
1078 425 180
TatI w'GTAC_w
Cuts at: 0 134 182 881 1000 1475 1683
Size: 134 48 699 119 475 208
Fragments arranged by size:
699 475 208 134 119 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 425 457 1683
Size: 425 32 1226
Fragments arranged by size:
1226 425 32
XbaI T'CTAG_A
Cuts at: 0 832 1683
Size: 832 851
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 984 1683
Size: 984 699
XmnI GAAnn'nnTTC
Cuts at: 0 1173 1683
Size: 1173 510
Enzymes that do cut:
AarI AcuI AflIII AleI AlwNI ApoI AvaI AvrII
BaeI BamHI BanII BbsI BcgI BciVI BglII BlpI
BmrI BplI BpmI BpuEI BsaWI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrGI BssSI BstXI
BstYI Bsu36I BtgI BtsI CspCI EaeI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI Hin4I MscI MslI MspA1I
NspI PasI PciI PfoI PpuMI PstI SacI ScaI
SexAI SfcI SmlI SspI StuI StyI TatI TstI
XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI BanI
BbeI BbvCI BclI BfrBI BglI Bme1580I BmgBI BmtI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrFI BssHII BstAPI BstBI
BstEII BstZ17I BtgZI ClaI DraI DraIII DrdI EagI
EcoNI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI TaqII Tth111I XhoI XmaI
ZraI