(Circular) (Six-base) MAPSORT of: pFN21AB3632.seq Check: 7907 from: 1 to: 5806 pFN21AB3632 5806 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3616 278 Size: 53 83 186 936 2080 2468 Fragments arranged by size: 2468 2080 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3149 1937 Size: 1212 4594 Acc65I G'GTAC_C Cuts at: 2305 3127 2305 Size: 822 4984 AclI AA'CG_TT Cuts at: 1563 3105 3933 4306 1563 Size: 1542 828 373 3063 Fragments arranged by size: 3063 1542 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2066 3869 5253 5521 1632 Size: 434 1803 1384 268 1917 Fragments arranged by size: 1917 1803 1384 434 268 AfeI AGC'GCT Cuts at: 2540 2540 Size: 5806 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5609 AflIII A'CryG_T Cuts at: 1203 2177 2446 3049 4705 5682 1203 Size: 974 269 603 1656 977 1327 Fragments arranged by size: 1656 1327 977 974 603 269 AgeI A'CCGG_T Cuts at: 4637 4637 Size: 5806 AhdI GACnn_n'nnGTC Cuts at: 4535 4535 Size: 5806 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5806 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5806 AlwNI CAG_nnn'CTG Cuts at: 2221 2284 2977 3549 5121 2221 Size: 63 693 572 1572 2906 Fragments arranged by size: 2906 1572 693 572 63 ApaLI G'TGCA_C Cuts at: 3863 5019 3863 Size: 1156 4650 ApoI r'AATT_y Cuts at: 1493 3115 3341 3493 5602 1493 Size: 1622 226 152 2109 1697 Fragments arranged by size: 2109 1697 1622 226 152 AseI AT'TA_AT Cuts at: 160 1032 4360 160 Size: 872 3328 1606 Fragments arranged by size: 3328 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5806 AvaI C'yCGr_G Cuts at: 1870 1943 2151 2877 3131 1870 Size: 73 208 726 254 4545 Fragments arranged by size: 4545 726 254 208 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2289 2322 2289 Size: 33 5773 BamHI G'GATC_C Cuts at: 2371 3136 3503 2371 Size: 765 367 4674 Fragments arranged by size: 4674 765 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2305 2471 2633 2764 3056 Size: 325 217 627 518 166 162 131 292 Cuts at: 3056 3127 4582 618 Size: 71 1455 1842 Fragments arranged by size: 1842 1455 627 518 325 292 217 166 162 131 71 BanII G_rGCy'C Cuts at: 1391 3125 1391 Size: 1734 4072 BbeI G_GCGC'C Cuts at: 3060 3060 Size: 5806 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2900 928 Size: 971 1001 3834 Fragments arranged by size: 3834 1001 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3997 4031 1903 Size: 34 2060 34 3678 Fragments arranged by size: 3678 2060 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3698 4908 3698 Size: 1210 4596 BclI T'GATC_A Cuts at: 1548 2778 1548 Size: 1230 4576 BfrBI ATG'CAT Cuts at: 2939 2939 Size: 5806 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2121 4417 136 Size: 107 122 71 631 605 449 2296 1525 Fragments arranged by size: 2296 1525 631 605 449 122 107 71 BglII A'GATC_T Cuts at: 5801 5801 Size: 5806 BlpI GC'TnA_GC Cuts at: 3223 3223 Size: 5806 Bme1580I G_kGCm'C Cuts at: 1790 2476 2638 2775 3867 5023 1790 Size: 686 162 137 1092 1156 2573 Fragments arranged by size: 2573 1156 1092 686 162 137 BmgBI CAC'GTC Cuts at: 1318 2447 3089 1318 Size: 1129 642 4035 Fragments arranged by size: 4035 1129 642 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2294 3537 4495 Size: 416 53 83 399 24 877 1243 958 Cuts at: 4495 442 Size: 1753 Fragments arranged by size: 1753 1243 958 877 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5806 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2831 4466 1239 Size: 1592 1635 2579 Fragments arranged by size: 2579 1635 1592 Bpu10I CC'TnA_GC Cuts at: 2529 2790 2529 Size: 261 5545 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2500 3931 4796 5094 2500 Size: 1431 865 298 3212 Fragments arranged by size: 3212 1431 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2548 4469 882 Size: 617 1049 1921 2219 Fragments arranged by size: 2219 1921 1049 617 BsaAI yAC'GTr Cuts at: 493 1204 2178 3597 493 Size: 711 974 1419 2702 Fragments arranged by size: 2702 1419 974 711 BsaBI GATnn'nnATC Cuts at: 5800 5800 Size: 5806 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3057 3613 3995 4630 Size: 53 83 186 936 1524 556 382 635 Cuts at: 4630 275 Size: 1451 Fragments arranged by size: 1524 1451 936 635 556 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2038 4239 4637 4911 5058 5469 2038 Size: 2201 398 274 147 411 2375 Fragments arranged by size: 2375 2201 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5581 Fragments arranged by size: 5581 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2828 2960 2963 1186 Size: 1642 132 3 4029 Fragments arranged by size: 4029 1642 132 3 BseYI C'CCAG_C Cuts at: 2321 5009 5505 2321 Size: 2688 496 2622 Fragments arranged by size: 2688 2622 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5233 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3256 4017 5045 1540 Size: 275 186 1255 761 1028 2301 Fragments arranged by size: 2301 1255 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3867 3952 5023 3867 Size: 85 1071 4650 Fragments arranged by size: 4650 1071 85 BsmI GAATG_Cn' Cuts at: 3317 3410 3317 Size: 93 5713 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2476 2638 2775 3125 3867 3952 5023 Size: 399 686 162 137 350 742 85 1071 Cuts at: 5023 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 686 399 350 162 137 85 BspEI T'CCGG_A Cuts at: 2038 5469 2038 Size: 3431 2375 BspHI T'CATG_A Cuts at: 3695 5425 3695 Size: 1730 4076 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3161 844 Size: 2317 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3693 1394 Size: 429 1870 3507 Fragments arranged by size: 3507 1870 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2642 2903 3003 4301 4475 65 Size: 1183 1394 261 100 1298 174 1396 Fragments arranged by size: 1396 1394 1298 1183 261 174 100 BsrFI r'CCGG_y Cuts at: 1700 1810 4450 4637 1700 Size: 110 2640 187 2869 Fragments arranged by size: 2869 2640 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5806 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5806 BssSI C'ACGA_G Cuts at: 3866 4878 3866 Size: 1012 4794 BstAPI GCAn_nnn'nTGC Cuts at: 794 2221 794 Size: 1427 4379 BstBI TT'CG_AA Cuts at: 4610 4610 Size: 5806 BstEII G'GTnAC_C Cuts at: 1184 2296 1184 Size: 1112 4694 BstYI r'GATC_y Cuts at: 1972 2371 3136 3503 3888 3905 5357 5443 5455 Size: 399 765 367 385 17 1452 86 12 Cuts at: 5455 5801 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 765 399 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2028 2190 2559 2028 Size: 162 369 5275 Fragments arranged by size: 5275 369 162 BtgI C'CryG_G Cuts at: 513 1065 2004 2301 2477 2523 3019 513 Size: 552 939 297 176 46 496 3300 Fragments arranged by size: 3300 939 552 496 297 176 46 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2981 487 Size: 683 56 1755 3312 Fragments arranged by size: 3312 1755 683 56 BtsI GCAGTG_nn' Cuts at: 2219 2702 3322 4116 4136 2219 Size: 483 620 794 20 3889 Fragments arranged by size: 3889 794 620 483 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5806 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5771 DraI TTT'AAA Cuts at: 3110 3110 Size: 5806 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4813 809 Size: 507 3497 1802 Fragments arranged by size: 3497 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2724 3253 4142 Size: 54 1425 50 275 18 894 529 889 Cuts at: 4142 5771 8 Size: 1629 43 Fragments arranged by size: 1629 1425 894 889 529 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3253 1537 Size: 275 1441 4090 Fragments arranged by size: 4090 1441 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2340 2693 3736 1353 Size: 987 353 1043 3423 Fragments arranged by size: 3423 1043 987 353 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4393 4767 4913 387 Size: 1064 138 2804 374 146 1280 Fragments arranged by size: 2804 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 2831 3869 4466 5253 5521 1239 Size: 393 434 765 1038 597 787 268 1524 Fragments arranged by size: 1524 1038 787 765 597 434 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2313 2313 Size: 5806 EcoO109I rG'GnC_Cy Cuts at: 2024 2318 2353 2024 Size: 294 35 5477 Fragments arranged by size: 5477 294 35 EcoRI G'AATT_C Cuts at: 3115 3493 3115 Size: 378 5428 FspI TGC'GCA Cuts at: 4312 4312 Size: 5806 HaeII r_GCGC'y Cuts at: 1678 2542 3060 4953 1678 Size: 864 518 1893 2531 Fragments arranged by size: 2531 1893 864 518 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4447 4479 4521 4553 1373 Size: 32 135 32 2875 32 42 32 2626 Fragments arranged by size: 2875 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3150 3396 669 Size: 988 281 1212 246 3079 Fragments arranged by size: 3079 1212 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5806 HpaI GTT'AAC Cuts at: 3396 3396 Size: 5806 KasI G'GCGC_C Cuts at: 3056 3056 Size: 5806 KpnI G_GTAC'C Cuts at: 2309 3131 2309 Size: 822 4984 MfeI C'AATT_G Cuts at: 3405 3405 Size: 5806 MluI A'CGCG_T Cuts at: 5682 5682 Size: 5806 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2283 4920 5104 1305 Size: 978 2637 184 2007 Fragments arranged by size: 2637 2007 978 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2726 10 Size: 54 1425 343 894 3090 Fragments arranged by size: 3090 1425 894 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2294 3764 4123 4282 518 Size: 648 1128 1470 359 159 2042 Fragments arranged by size: 2042 1470 1128 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2455 2575 2650 3222 3899 5047 5292 1606 Size: 849 120 75 572 677 1148 245 2120 Fragments arranged by size: 2120 1148 849 677 572 245 120 75 NarI GG'CG_CC Cuts at: 3057 3057 Size: 5806 NcoI C'CATG_G Cuts at: 513 1065 2004 2301 2477 2523 3019 513 Size: 552 939 297 176 46 496 3300 Fragments arranged by size: 3300 939 552 496 297 176 46 NdeI CA'TA_TG Cuts at: 387 387 Size: 5806 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5806 NotI GC'GGCC_GC Cuts at: 3253 3253 Size: 5806 NsiI A_TGCA'T Cuts at: 2941 2941 Size: 5806 NspI r_CATG'y Cuts at: 1132 1229 2113 3053 3164 4709 1132 Size: 97 884 940 111 1545 2229 Fragments arranged by size: 2229 1545 940 884 111 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5806 PciI A'CATG_T Cuts at: 3049 4705 3049 Size: 1656 4150 PflMI CCAn_nnn'nTGG Cuts at: 1108 2284 2541 1108 Size: 1176 257 4373 Fragments arranged by size: 4373 1176 257 PmeI GTTT'AAAC Cuts at: 3110 3110 Size: 5806 PmlI CAC'GTG Cuts at: 2178 2178 Size: 5806 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3875 3907 5388 5420 3875 Size: 32 1481 32 4261 Fragments arranged by size: 4261 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2024 2318 2353 2024 Size: 294 35 5477 Fragments arranged by size: 5477 294 35 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5806 PsiI TTA'TAA Cuts at: 3376 3376 Size: 5806 PstI C_TGCA'G Cuts at: 830 2217 3158 830 Size: 1387 941 3478 Fragments arranged by size: 3478 1387 941 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5806 PvuII CAG'CTG Cuts at: 2575 2650 2575 Size: 75 5731 SalI G'TCGA_C Cuts at: 1936 3148 1936 Size: 1212 4594 SapI GCTCTTCn'nnn_ Cuts at: 2340 2693 2340 Size: 353 5453 SbfI CC_TGCA'GG Cuts at: 3158 3158 Size: 5806 ScaI AGT'ACT Cuts at: 4054 5766 4054 Size: 1712 4094 SfcI C'TryA_G Cuts at: 826 1045 2213 3154 4289 4970 5161 826 Size: 219 1168 941 1135 681 191 1471 Fragments arranged by size: 1471 1168 1135 941 681 219 191 SfoI GGC'GCC Cuts at: 3058 3058 Size: 5806 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5806 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5806 SmaI CCC'GGG Cuts at: 1872 2153 2879 3133 1872 Size: 281 726 254 4545 Fragments arranged by size: 4545 726 281 254 SmlI C'TyrA_G Cuts at: 820 1017 1943 2515 3910 4811 5073 820 Size: 197 926 572 1395 901 262 1553 Fragments arranged by size: 1553 1395 926 901 572 262 197 SnaBI TAC'GTA Cuts at: 493 3597 493 Size: 3104 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5806 SphI G_CATG'C Cuts at: 1132 2113 3164 1132 Size: 981 1051 3774 Fragments arranged by size: 3774 1051 981 SspI AAT'ATT Cuts at: 5 52 3730 5 Size: 47 3678 2081 Fragments arranged by size: 3678 2081 47 StuI AGG'CCT Cuts at: 1517 2195 2760 1517 Size: 678 565 4563 Fragments arranged by size: 4563 678 565 StyI C'CwwG_G Cuts at: 513 1065 2004 2301 2477 2523 3019 3245 513 Size: 552 939 297 176 46 496 226 3074 Fragments arranged by size: 3074 939 552 496 297 226 176 46 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3833 3850 4003 4188 4633 325 Size: 1255 2253 17 153 185 445 1498 Fragments arranged by size: 2253 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4052 5764 96 Size: 274 80 33 51 1443 2075 1712 138 Fragments arranged by size: 2075 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2610 2642 5766 5798 2610 Size: 32 3124 32 2618 Fragments arranged by size: 3124 2618 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5806 XbaI T'CTAG_A Cuts at: 3142 3142 Size: 5806 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5806 XmaI C'CCGG_G Cuts at: 1870 2151 2877 3131 1870 Size: 281 726 254 4545 Fragments arranged by size: 4545 726 281 254 XmnI GAAnn'nnTTC Cuts at: 3935 3935 Size: 5806 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3614 276 Size: 53 83 186 936 2080 2468 Fragments arranged by size: 2468 2080 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbvCI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI