################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BanI BbeI BbrPI BcgI BfrI BlnI BlpI Bpu1102I BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BspDI BspEI BspLU11I BsrBI BsrFI BssHII BstAPI BstBI Bsu36I CelII Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FseI FspI HincII HindII HpaI KasI KpnI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI Sse8387I SwaI Tsp45I Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccII 21 CGCG CG^CG AlwNI 1981 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 272 GGGCCC GGGCC^C Asp700I 614 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1260 GTGCTC G[AT]GC[AT]^C BalI 83 TGGCCA TGG^CCA BanII 272 GGGCCC G[GA]GC[CT]^C BbvCI 1231 GCTGAGG CCTCAGC(-5/-2) BclI 1440 TGATCA T^GATCA BglI 58 GCCGCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 455 AGATCT A^GATCT BsaAI 1807 TACGTG [CT]AC^GT[GA] BsaBI 711 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 1260 GTGCTC G[AT]GC[AT]^C BsmAI 1983 GAGAC GTCTC(1/5) Bsp1407I 2054 TGTACA T^GTACA BspHI 954 TCATGA T^CATGA BsrGI 2054 TGTACA T^GTACA BssSI 403 CTCGTG CACGAG(-5/-1) BstEII 748 GGTTACC G^GT[ACGT]ACC BstPI 748 GGTTACC G^GT[ACGT]ACC BstUI 21 CGCG CG^CG DraI 1348 TTTAAA TTT^AAA DraII 272 GGGCCCC [GA]G^G[ACGT]CC[CT] DraIII 1203 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 83 TGGCCA [CT]^GGCC[GA] EarI 799 GAAGAG CTCTTC(1/4) EcoO109I 272 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoO65I 748 GGTTACC G^GT[ACGT]ACC FauI 19 CCCGC CCCGC(4/6) FbaI 1440 TGATCA T^GATCA HapII 277 CCGG C^CGG HindIII 762 AAGCTT A^AGCTT HpaII 277 CCGG C^CGG Ksp632I 799 GAAGAG CTCTTC(1/4) KspI 20 CCGCGG CCGC^GG MamI 711 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 83 TGGCCA TGG^CCA MscI 83 TGGCCA TGG^CCA MspI 277 CCGG C^CGG MvnI 21 CGCG CG^CG NdeI 548 CATATG CA^TATG PflMI 1098 CCATGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 990 CAGCTG CAG^CTG RcaI 954 TCATGA T^CATGA SacII 20 CCGCGG CCGC^GG SapI 799 GAAGAGC GCTCTTC(1/4) SmaI 276 CCCGGG CCC^GGG SpeI 439 ACTAGT A^CTAGT SphI 726 GCATGC GCATG^C SspBI 2054 TGTACA T^GTACA SspI 1136 AATATT AAT^ATT Van91I 1098 CCATGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1275 CCAGGGAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 276 CCCGGG C^CCGGG XmaI 276 CCCGGG C^CCGGG XmnI 614 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcyI 229 GGCGTC G[GA]^CG[CT]C 2033 GGCGTC AvaI 31 CCCGAG C^[CT]CG[GA]G 276 CCCGGG AvaII 940 GGACC G^G[AT]CC 1989 GGACC BamHI 415 GGATCC G^GATCC 534 GGATCC BbsI 1357 GAAGAC GAAGAC(2/6) 1859 GTCTTC BciVI 524 GGATAC GTATCC(6/5) 691 GGATAC BcnI 276 CCCGG CC^[GC]GG 277 CCGGG BpuAI 1357 GAAGAC GAAGAC(2/6) 1859 GTCTTC BsaHI 229 GGCGTC G[GA]^CG[CT]C 2033 GGCGTC BsoBI 31 CCCGAG C^[CT]CG[GA]G 276 CCCGGG BspMI 140 GCAGGT ACCTGC(4/8) 1855 GCAGGT Bst1107I 1381 GTATAC GTA^TAC 1497 GTATAC BstXI 86 CCAGACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1318 CCAGATAAGTGG BstZ17I 1381 GTATAC GTA^TAC 1497 GTATAC HaeII 48 GGCGCT [GA]GCGC^[CT] 1400 GGCGCT HgaI 230 GCGTC GACGC(5/10) 2034 GCGTC Hin1I 229 GGCGTC G[GA]^CG[CT]C 2033 GGCGTC NciI 276 CCCGG CC^[GC]GG 277 CCGGG NspI 658 ACATGC [GA]CATG^[CT] 726 GCATGC StuI 684 AGGCCT AGG^CCT 1802 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI ApoI BbvI BfaI BmyI BpmI BsaJI BsiYI BslI BsmFI BsmI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EcoRII EcoT14I Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NcoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII