(Circular) (Six-base) MAPSORT of: pFN21AE4565.seq Check: 9042 from: 1 to: 6811 pFN21AE4565 6811 bp With 182 enzymes: SgfI * March 28, 2012 14:16 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4621 278 Size: 53 83 186 936 3085 2468 Fragments arranged by size: 3085 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2823 3387 3503 4154 1937 Size: 886 564 116 651 4594 Fragments arranged by size: 4594 886 651 564 116 Acc65I G'GTAC_C Cuts at: 4132 4132 Size: 6811 AclI AA'CG_TT Cuts at: 1563 4938 5311 1563 Size: 3375 373 3063 Fragments arranged by size: 3375 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2823 4073 4874 6258 6526 1632 Size: 1191 1250 801 1384 268 1917 Fragments arranged by size: 1917 1384 1250 1191 801 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6614 AflIII A'CryG_T Cuts at: 1203 5710 6687 1203 Size: 4507 977 1327 Fragments arranged by size: 4507 1327 977 AgeI A'CCGG_T Cuts at: 5642 5642 Size: 6811 AhdI GACnn_n'nnGTC Cuts at: 5540 5540 Size: 6811 AleI CACnn'nnGTG Cuts at: 1166 2244 1166 Size: 1078 5733 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6811 AlwNI CAG_nnn'CTG Cuts at: 3991 4554 6126 3991 Size: 563 1572 4676 Fragments arranged by size: 4676 1572 563 ApaI G_GGCC'C Cuts at: 2281 2281 Size: 6811 ApaLI G'TGCA_C Cuts at: 4868 6024 4868 Size: 1156 5655 ApoI r'AATT_y Cuts at: 1493 2166 2598 2619 2887 3629 3938 4120 4346 Size: 673 432 21 268 742 309 182 226 Cuts at: 4346 4498 6607 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 742 673 432 309 268 226 182 152 21 AseI AT'TA_AT Cuts at: 160 1032 5365 160 Size: 872 4333 1606 Fragments arranged by size: 4333 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6811 AvaI C'yCGr_G Cuts at: 1870 1943 2036 2281 4136 1870 Size: 73 93 245 1855 4545 Fragments arranged by size: 4545 1855 245 93 73 BamHI G'GATC_C Cuts at: 2420 2539 4141 4508 2420 Size: 119 1602 367 4723 Fragments arranged by size: 4723 1602 367 119 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4132 5587 618 Size: 325 217 627 2345 1455 1842 Fragments arranged by size: 2345 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2281 4130 1391 Size: 890 1849 4072 Fragments arranged by size: 4072 1849 890 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3369 3857 928 Size: 971 1470 488 3882 Fragments arranged by size: 3882 1470 971 488 BbvCI CC'TCA_GC Cuts at: 3237 3237 Size: 6811 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5002 5036 1903 Size: 34 3065 34 3678 Fragments arranged by size: 3678 3065 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2523 2690 4703 5913 2523 Size: 167 2013 1210 3421 Fragments arranged by size: 3421 2013 1210 167 BclI T'GATC_A Cuts at: 1548 3445 1548 Size: 1897 4914 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2069 5422 Size: 107 122 71 631 605 337 60 3353 Cuts at: 5422 136 Size: 1525 Fragments arranged by size: 3353 1525 631 605 337 122 107 71 60 BglII A'GATC_T Cuts at: 2460 6806 2460 Size: 4346 2465 BlpI GC'TnA_GC Cuts at: 4228 4228 Size: 6811 Bme1580I G_kGCm'C Cuts at: 1790 2082 2281 4872 6028 1790 Size: 292 199 2591 1156 2573 Fragments arranged by size: 2591 2573 1156 292 199 BmgBI CAC'GTC Cuts at: 1318 2077 1318 Size: 759 6052 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2545 4542 5500 Size: 416 53 83 399 24 1128 1997 958 Cuts at: 5500 442 Size: 1753 Fragments arranged by size: 1997 1753 1128 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6811 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4097 4097 Size: 6811 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2120 2328 2977 3519 5471 1239 Size: 881 208 649 542 1952 2579 Fragments arranged by size: 2579 1952 881 649 542 208 Bpu10I CC'TnA_GC Cuts at: 3237 3237 Size: 6811 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4936 5801 6099 4936 Size: 865 298 5648 Fragments arranged by size: 5648 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5474 882 Size: 617 3975 2219 Fragments arranged by size: 3975 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3814 4602 493 Size: 711 2610 788 2702 Fragments arranged by size: 2702 2610 788 711 BsaBI GATnn'nnATC Cuts at: 2720 6805 2720 Size: 4085 2726 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2235 4039 4618 5000 Size: 53 83 186 936 702 1804 579 382 Cuts at: 5000 5635 275 Size: 635 1451 Fragments arranged by size: 1804 1451 936 702 635 579 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5244 5642 5916 6063 6474 5244 Size: 398 274 147 411 5581 Fragments arranged by size: 5581 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6586 Fragments arranged by size: 6586 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6811 BseYI C'CCAG_C Cuts at: 2997 6014 6510 2997 Size: 3017 496 3298 Fragments arranged by size: 3298 3017 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6238 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4261 5022 6050 1540 Size: 275 186 2260 761 1028 2301 Fragments arranged by size: 2301 2260 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3269 4872 4957 6028 3269 Size: 1603 85 1071 4052 Fragments arranged by size: 4052 1603 1071 85 BsmI GAATG_Cn' Cuts at: 2566 2706 3258 4322 4415 2566 Size: 140 552 1064 93 4962 Fragments arranged by size: 4962 1064 552 140 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 2281 3269 4130 4872 4957 6028 Size: 399 292 199 988 861 742 85 1071 Cuts at: 6028 1391 Size: 2174 Fragments arranged by size: 2174 1071 988 861 742 399 292 199 85 BspEI T'CCGG_A Cuts at: 6474 6474 Size: 6811 BspHI T'CATG_A Cuts at: 2959 4700 6430 2959 Size: 1741 1730 3340 Fragments arranged by size: 3340 1741 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2136 3851 4166 844 Size: 1292 1715 315 3489 Fragments arranged by size: 3489 1715 1292 315 BsrBI CCG'CTC Cuts at: 1394 1823 4698 1394 Size: 429 2875 3507 Fragments arranged by size: 3507 2875 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2674 3423 3843 3926 5306 5480 65 Size: 1183 1426 749 420 83 1380 174 1396 Fragments arranged by size: 1426 1396 1380 1183 749 420 174 83 BsrFI r'CCGG_y Cuts at: 1700 1810 5455 5642 1700 Size: 110 3645 187 2869 Fragments arranged by size: 3645 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4059 96 Size: 3963 2848 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6811 BssSI C'ACGA_G Cuts at: 2408 4871 5883 2408 Size: 2463 1012 3336 Fragments arranged by size: 3336 2463 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6811 BstBI TT'CG_AA Cuts at: 5615 5615 Size: 6811 BstEII G'GTnAC_C Cuts at: 1184 2753 1184 Size: 1569 5242 BstXI CCAn_nnnn'nTGG Cuts at: 2098 3330 2098 Size: 1232 5579 BstYI r'GATC_y Cuts at: 1972 2420 2460 2539 4099 4141 4508 4893 4910 Size: 448 40 79 1560 42 367 385 17 Cuts at: 4910 6362 6448 6460 6806 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1560 1452 448 385 367 346 86 79 42 40 17 12 BstZ17I GTA'TAC Cuts at: 3388 3504 3388 Size: 116 6695 BtgI C'CryG_G Cuts at: 513 1065 2004 2025 2130 2480 2585 2838 3103 Size: 552 939 21 105 350 105 253 265 Cuts at: 3103 3312 513 Size: 209 4012 Fragments arranged by size: 4012 939 552 350 265 253 209 105 105 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6072 Fragments arranged by size: 6072 683 56 BtsI GCAGTG_nn' Cuts at: 4327 5121 5141 4327 Size: 794 20 5997 Fragments arranged by size: 5997 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6811 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2475 2510 4056 4091 529 Size: 35 1911 35 1546 35 3249 Fragments arranged by size: 3249 1911 1546 35 35 35 DraI TTT'AAA Cuts at: 3355 4115 3355 Size: 760 6051 DraIII CAC_nnn'GTG Cuts at: 3213 3213 Size: 6811 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5818 809 Size: 507 4502 1802 Fragments arranged by size: 4502 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2088 4258 5147 Size: 54 1425 50 275 18 258 2170 889 Cuts at: 5147 6776 8 Size: 1629 43 Fragments arranged by size: 2170 1629 1425 889 275 258 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4258 1537 Size: 275 2446 4090 Fragments arranged by size: 4090 2446 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2799 4741 1353 Size: 1446 1942 3423 Fragments arranged by size: 3423 1942 1446 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5398 5772 5918 387 Size: 1064 138 3809 374 146 1280 Fragments arranged by size: 3809 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2120 2328 2823 2977 3519 4073 4874 Size: 393 488 208 495 154 542 554 801 Cuts at: 4874 5471 6258 6526 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 801 787 597 554 542 495 488 393 268 208 154 EcoO109I rG'GnC_Cy Cuts at: 2278 2278 Size: 6811 EcoRI G'AATT_C Cuts at: 4120 4498 4120 Size: 378 6433 FspI TGC'GCA Cuts at: 5317 5317 Size: 6811 HaeII r_GCGC'y Cuts at: 1678 2057 3409 5958 1678 Size: 379 1352 2549 2531 Fragments arranged by size: 2549 2531 1352 379 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2452 2484 2947 2979 3230 Size: 32 135 32 880 32 463 32 251 Cuts at: 3230 3262 3430 3462 5452 5484 5526 5558 1373 Size: 32 168 32 1990 32 42 32 2626 Fragments arranged by size: 2626 1990 880 463 251 168 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4155 4401 669 Size: 988 281 2217 246 3079 Fragments arranged by size: 3079 2217 988 281 246 HindIII A'AGCT_T Cuts at: 748 2767 748 Size: 2019 4792 HpaI GTT'AAC Cuts at: 4401 4401 Size: 6811 KpnI G_GTAC'C Cuts at: 4136 4136 Size: 6811 MfeI C'AATT_G Cuts at: 4410 4410 Size: 6811 MluI A'CGCG_T Cuts at: 6687 6687 Size: 6811 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2303 3021 3898 5925 6109 1305 Size: 998 718 877 2027 184 2007 Fragments arranged by size: 2027 2007 998 877 718 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2090 10 Size: 54 1425 343 258 4731 Fragments arranged by size: 4731 1425 343 258 54 MslI CAynn'nnrTG Cuts at: 518 1166 2244 2553 2874 3102 4769 5128 5287 Size: 648 1078 309 321 228 1667 359 159 Cuts at: 5287 518 Size: 2042 Fragments arranged by size: 2042 1667 1078 648 359 321 309 228 159 MspA1I CmG'CkG Cuts at: 1606 2027 2997 3968 4227 4904 6052 6297 1606 Size: 421 970 971 259 677 1148 245 2120 Fragments arranged by size: 2120 1148 971 970 677 421 259 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2838 3103 3312 513 Size: 552 939 834 265 209 4012 Fragments arranged by size: 4012 939 834 552 265 209 NdeI CA'TA_TG Cuts at: 387 2554 387 Size: 2167 4644 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6811 NotI GC'GGCC_GC Cuts at: 4258 4258 Size: 6811 NspI r_CATG'y Cuts at: 1132 1229 2667 2735 4169 5714 1132 Size: 97 1438 68 1434 1545 2229 Fragments arranged by size: 2229 1545 1438 1434 97 68 PasI CC'CwG_GG Cuts at: 1348 3280 1348 Size: 1932 4879 PciI A'CATG_T Cuts at: 5710 5710 Size: 6811 PflMI CCAn_nnn'nTGG Cuts at: 1108 3109 1108 Size: 2001 4810 PfoI T'CCnGG_A Cuts at: 2097 2097 Size: 6811 PmeI GTTT'AAAC Cuts at: 4115 4115 Size: 6811 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4880 4912 6393 6425 4880 Size: 32 1481 32 5266 Fragments arranged by size: 5266 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6811 PsiI TTA'TAA Cuts at: 2652 4381 2652 Size: 1729 5082 PspOMI G'GGCC_C Cuts at: 2277 2277 Size: 6811 PstI C_TGCA'G Cuts at: 830 4163 830 Size: 3333 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6811 PvuII CAG'CTG Cuts at: 2997 2997 Size: 6811 SacII CC_GC'GG Cuts at: 2028 2028 Size: 6811 SalI G'TCGA_C Cuts at: 1936 4153 1936 Size: 2217 4594 SapI GCTCTTCn'nnn_ Cuts at: 2799 2799 Size: 6811 SbfI CC_TGCA'GG Cuts at: 4163 4163 Size: 6811 ScaI AGT'ACT Cuts at: 5059 6771 5059 Size: 1712 5099 SfcI C'TryA_G Cuts at: 826 1045 4159 5294 5975 6166 826 Size: 219 3114 1135 681 191 1471 Fragments arranged by size: 3114 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6811 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6811 SmaI CCC'GGG Cuts at: 1872 2283 4138 1872 Size: 411 1855 4545 Fragments arranged by size: 4545 1855 411 SmlI C'TyrA_G Cuts at: 820 1017 1943 4915 5816 6078 820 Size: 197 926 2972 901 262 1553 Fragments arranged by size: 2972 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4602 493 Size: 4109 2702 SpeI A'CTAG_T Cuts at: 152 2444 152 Size: 2292 4519 SphI G_CATG'C Cuts at: 1132 2735 4169 1132 Size: 1603 1434 3774 Fragments arranged by size: 3774 1603 1434 SspI AAT'ATT Cuts at: 5 52 3143 4735 5 Size: 47 3091 1592 2081 Fragments arranged by size: 3091 2081 1592 47 StuI AGG'CCT Cuts at: 1517 2691 3809 1517 Size: 1174 1118 4519 Fragments arranged by size: 4519 1174 1118 StyI C'CwwG_G Cuts at: 513 1065 2004 2692 2838 3103 3312 4250 513 Size: 552 939 688 146 265 209 938 3074 Fragments arranged by size: 3074 939 938 688 552 265 209 146 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4838 4855 5008 5193 5638 325 Size: 1255 3258 17 153 185 445 1498 Fragments arranged by size: 3258 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2404 2671 4059 Size: 274 80 33 51 1443 427 267 1388 Cuts at: 4059 5057 6769 96 Size: 998 1712 138 Fragments arranged by size: 1712 1443 1388 998 427 274 267 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3199 3231 6771 6803 3199 Size: 32 3540 32 3207 Fragments arranged by size: 3540 3207 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6811 XbaI T'CTAG_A Cuts at: 4147 4147 Size: 6811 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3287 3287 Size: 6811 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6811 XmaI C'CCGG_G Cuts at: 1870 2281 4136 1870 Size: 411 1855 4545 Fragments arranged by size: 4545 1855 411 XmnI GAAnn'nnTTC Cuts at: 2623 4940 2623 Size: 2317 4494 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4619 276 Size: 53 83 186 936 3085 2468 Fragments arranged by size: 3085 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI