(Circular) (Six-base) MAPSORT of: pFN21AB3604.seq Check: 9027 from: 1 to: 6205 pFN21AB3604 6205 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4015 278 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2109 3548 1937 Size: 172 1439 4594 Fragments arranged by size: 4594 1439 172 Acc65I G'GTAC_C Cuts at: 2538 3279 3526 2538 Size: 741 247 5217 Fragments arranged by size: 5217 741 247 AclI AA'CG_TT Cuts at: 1563 4332 4705 1563 Size: 2769 373 3063 Fragments arranged by size: 3063 2769 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3052 3427 4268 5652 5920 1632 Size: 1420 375 841 1384 268 1917 Fragments arranged by size: 1917 1420 1384 841 375 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6008 AflIII A'CryG_T Cuts at: 1203 5104 6081 1203 Size: 3901 977 1327 Fragments arranged by size: 3901 1327 977 AgeI A'CCGG_T Cuts at: 5036 5036 Size: 6205 AhdI GACnn_n'nnGTC Cuts at: 4934 4934 Size: 6205 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6205 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2297 1184 Size: 1113 5092 AlwNI CAG_nnn'CTG Cuts at: 2032 2213 3948 5520 2032 Size: 181 1735 1572 2717 Fragments arranged by size: 2717 1735 1572 181 ApaI G_GGCC'C Cuts at: 3025 3025 Size: 6205 ApaLI G'TGCA_C Cuts at: 4262 5418 4262 Size: 1156 5049 ApoI r'AATT_y Cuts at: 1493 2117 3514 3740 3892 6001 1493 Size: 624 1397 226 152 2109 1697 Fragments arranged by size: 2109 1697 1397 624 226 152 AseI AT'TA_AT Cuts at: 160 1032 4759 160 Size: 872 3727 1606 Fragments arranged by size: 3727 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 AvaI C'yCGr_G Cuts at: 1870 1943 3530 1870 Size: 73 1587 4545 Fragments arranged by size: 4545 1587 73 BamHI G'GATC_C Cuts at: 2075 3535 3902 2075 Size: 1460 367 4378 Fragments arranged by size: 4378 1460 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2068 2538 3279 3314 3526 Size: 325 217 627 281 470 741 35 212 Cuts at: 3526 4981 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 741 627 470 325 281 217 212 35 BanII G_rGCy'C Cuts at: 1391 2886 3025 3524 1391 Size: 1495 139 499 4072 Fragments arranged by size: 4072 1495 499 139 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2150 2864 3433 928 Size: 971 251 714 569 3700 Fragments arranged by size: 3700 971 714 569 251 BbvCI CC'TCA_GC Cuts at: 3183 3183 Size: 6205 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4396 4430 1903 Size: 34 2459 34 3678 Fragments arranged by size: 3678 2459 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4097 5307 4097 Size: 1210 4995 BclI T'GATC_A Cuts at: 1548 1548 Size: 6205 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3242 4816 136 Size: 107 122 71 631 605 1570 1574 1525 Fragments arranged by size: 1574 1570 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2165 2301 6200 2165 Size: 136 3899 2170 Fragments arranged by size: 3899 2170 136 BlpI GC'TnA_GC Cuts at: 2677 3622 2677 Size: 945 5260 Bme1580I G_kGCm'C Cuts at: 1790 3025 4266 5422 1790 Size: 1235 1241 1156 2573 Fragments arranged by size: 2573 1241 1235 1156 BmgBI CAC'GTC Cuts at: 1318 2592 3058 1318 Size: 1274 466 4465 Fragments arranged by size: 4465 1274 466 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3043 3096 3280 Size: 416 53 83 399 24 1626 53 184 Cuts at: 3280 3936 4894 442 Size: 656 958 1753 Fragments arranged by size: 1753 1626 958 656 416 399 184 83 53 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6205 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2606 3254 4865 1239 Size: 1367 648 1611 2579 Fragments arranged by size: 2579 1611 1367 648 Bpu10I CC'TnA_GC Cuts at: 3183 3183 Size: 6205 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4330 5195 5493 4330 Size: 865 298 5042 Fragments arranged by size: 5042 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4868 882 Size: 617 3369 2219 Fragments arranged by size: 3369 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3996 493 Size: 711 2792 2702 Fragments arranged by size: 2792 2702 711 BsaBI GATnn'nnATC Cuts at: 2714 6199 2714 Size: 3485 2720 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4012 4394 5029 275 Size: 53 83 186 936 2479 382 635 1451 Fragments arranged by size: 2479 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2410 2541 2993 3132 4638 5036 5310 5457 5868 Size: 131 452 139 1506 398 274 147 411 Cuts at: 5868 2410 Size: 2747 Fragments arranged by size: 2747 1506 452 411 398 274 147 139 131 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5980 Fragments arranged by size: 5980 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2296 2594 2813 3012 3122 1186 Size: 1110 298 219 199 110 4269 Fragments arranged by size: 4269 1110 298 219 199 110 BseYI C'CCAG_C Cuts at: 2025 2472 3049 3151 5408 5904 2025 Size: 447 577 102 2257 496 2326 Fragments arranged by size: 2326 2257 577 496 447 102 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2989 1163 Size: 573 1253 4379 Fragments arranged by size: 4379 1253 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3655 4416 5444 1540 Size: 275 186 1654 761 1028 2301 Fragments arranged by size: 2301 1654 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3033 4266 4351 5422 3033 Size: 1233 85 1071 3816 Fragments arranged by size: 3816 1233 1071 85 BsmI GAATG_Cn' Cuts at: 2209 2286 3716 3809 2209 Size: 77 1430 93 4605 Fragments arranged by size: 4605 1430 93 77 BsmBI CGTCTCn'nnnn_ Cuts at: 2582 2582 Size: 6205 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2886 3025 3033 3524 4266 4351 5422 Size: 399 1096 139 8 491 742 85 1071 Cuts at: 5422 1391 Size: 2174 Fragments arranged by size: 2174 1096 1071 742 491 399 139 85 8 BspEI T'CCGG_A Cuts at: 2410 5868 2410 Size: 3458 2747 BspHI T'CATG_A Cuts at: 4094 5824 4094 Size: 1730 4475 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3222 3560 844 Size: 2378 338 3489 Fragments arranged by size: 3489 2378 338 BsrBI CCG'CTC Cuts at: 1394 1823 4092 1394 Size: 429 2269 3507 Fragments arranged by size: 3507 2269 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4700 4874 65 Size: 1183 3452 174 1396 Fragments arranged by size: 3452 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4849 5036 1700 Size: 110 3039 187 2869 Fragments arranged by size: 3039 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6205 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6205 BssSI C'ACGA_G Cuts at: 4265 5277 4265 Size: 1012 5193 BstAPI GCAn_nnn'nTGC Cuts at: 794 2443 3233 3321 794 Size: 1649 790 88 3678 Fragments arranged by size: 3678 1649 790 88 BstBI TT'CG_AA Cuts at: 5009 5009 Size: 6205 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6205 BstXI CCAn_nnnn'nTGG Cuts at: 2612 2612 Size: 6205 BstYI r'GATC_y Cuts at: 1972 2075 2165 2287 2301 2827 3135 3535 3902 Size: 103 90 122 14 526 308 400 367 Cuts at: 3902 4287 4304 5756 5842 5854 6200 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 526 400 385 367 346 308 122 103 90 86 17 14 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2484 2656 2667 2750 513 Size: 552 939 480 172 11 83 3968 Fragments arranged by size: 3968 939 552 480 172 83 11 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2722 2932 487 Size: 683 56 1496 210 3760 Fragments arranged by size: 3760 1496 683 210 56 BtsI GCAGTG_nn' Cuts at: 3721 4515 4535 3721 Size: 794 20 5391 Fragments arranged by size: 5391 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6205 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6170 DraI TTT'AAA Cuts at: 3509 3509 Size: 6205 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2088 5212 809 Size: 507 772 3124 1802 Fragments arranged by size: 3124 1802 772 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2865 3652 4541 Size: 54 1425 50 275 18 1035 787 889 Cuts at: 4541 6170 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1035 889 787 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3652 1537 Size: 275 1840 4090 Fragments arranged by size: 4090 1840 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2232 2766 2811 4135 1353 Size: 879 534 45 1324 3423 Fragments arranged by size: 3423 1324 879 534 45 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4792 5166 5312 387 Size: 1064 138 3203 374 146 1280 Fragments arranged by size: 3203 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2606 3052 3254 3427 4268 4865 5652 Size: 393 974 446 202 173 841 597 787 Cuts at: 5652 5920 1239 Size: 268 1524 Fragments arranged by size: 1524 974 841 787 597 446 393 268 202 173 EcoO109I rG'GnC_Cy Cuts at: 2159 2608 3037 3157 3211 3244 2159 Size: 449 429 120 54 33 5120 Fragments arranged by size: 5120 449 429 120 54 33 EcoRI G'AATT_C Cuts at: 3514 3892 3514 Size: 378 5827 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2545 2545 Size: 6205 FspI TGC'GCA Cuts at: 4711 4711 Size: 6205 HaeII r_GCGC'y Cuts at: 1678 2032 5352 1678 Size: 354 3320 2531 Fragments arranged by size: 3320 2531 354 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2379 2411 4846 4878 4920 Size: 32 135 32 807 32 2435 32 42 Cuts at: 4920 4952 1373 Size: 32 2626 Fragments arranged by size: 2626 2435 807 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2110 2383 3549 3795 669 Size: 988 281 172 273 1166 246 3079 Fragments arranged by size: 3079 1166 988 281 273 246 172 HindIII A'AGCT_T Cuts at: 748 748 Size: 6205 HpaI GTT'AAC Cuts at: 3795 3795 Size: 6205 KpnI G_GTAC'C Cuts at: 2542 3283 3530 2542 Size: 741 247 5217 Fragments arranged by size: 5217 741 247 MfeI C'AATT_G Cuts at: 3804 3804 Size: 6205 MluI A'CGCG_T Cuts at: 6081 6081 Size: 6205 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2377 5319 5503 1305 Size: 1072 2942 184 2007 Fragments arranged by size: 2942 2007 1072 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4383 Fragments arranged by size: 4383 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2483 2531 4163 4522 4681 518 Size: 648 1317 48 1632 359 159 2042 Fragments arranged by size: 2042 1632 1317 648 359 159 48 MspA1I CmG'CkG Cuts at: 1606 2188 2808 3621 4298 5446 5691 1606 Size: 582 620 813 677 1148 245 2120 Fragments arranged by size: 2120 1148 813 677 620 582 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2484 2656 2667 513 Size: 552 939 480 172 11 4051 Fragments arranged by size: 4051 939 552 480 172 11 NdeI CA'TA_TG Cuts at: 387 387 Size: 6205 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6205 NotI GC'GGCC_GC Cuts at: 3652 3652 Size: 6205 NspI r_CATG'y Cuts at: 1132 1229 2377 3563 5108 1132 Size: 97 1148 1186 1545 2229 Fragments arranged by size: 2229 1545 1186 1148 97 PasI CC'CwG_GG Cuts at: 1348 2156 1348 Size: 808 5397 PciI A'CATG_T Cuts at: 5104 5104 Size: 6205 PflMI CCAn_nnn'nTGG Cuts at: 1108 2032 1108 Size: 924 5281 PmeI GTTT'AAAC Cuts at: 3509 3509 Size: 6205 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4274 4306 5787 5819 4274 Size: 32 1481 32 4660 Fragments arranged by size: 4660 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3157 3211 3157 Size: 54 6151 PshAI GACnn'nnGTC Cuts at: 1613 2150 1613 Size: 537 5668 PsiI TTA'TAA Cuts at: 3775 3775 Size: 6205 PspOMI G'GGCC_C Cuts at: 3021 3021 Size: 6205 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3272 3304 3272 Size: 32 6173 PstI C_TGCA'G Cuts at: 830 2042 2101 3557 830 Size: 1212 59 1456 3478 Fragments arranged by size: 3478 1456 1212 59 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6205 PvuII CAG'CTG Cuts at: 2188 2188 Size: 6205 SalI G'TCGA_C Cuts at: 1936 2108 3547 1936 Size: 172 1439 4594 Fragments arranged by size: 4594 1439 172 SanDI GG'GwC_CC Cuts at: 3157 3157 Size: 6205 SbfI CC_TGCA'GG Cuts at: 3557 3557 Size: 6205 ScaI AGT'ACT Cuts at: 4453 6165 4453 Size: 1712 4493 SexAI A'CCwGG_T Cuts at: 2256 2256 Size: 6205 SfcI C'TryA_G Cuts at: 826 1045 2038 2097 2626 3553 4688 5369 5560 Size: 219 993 59 529 927 1135 681 191 Cuts at: 5560 826 Size: 1471 Fragments arranged by size: 1471 1135 993 927 681 529 219 191 59 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6205 SmaI CCC'GGG Cuts at: 1872 3532 1872 Size: 1660 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4309 5210 5472 820 Size: 197 926 2366 901 262 1553 Fragments arranged by size: 2366 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3996 493 Size: 3503 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6205 SphI G_CATG'C Cuts at: 1132 3563 1132 Size: 2431 3774 SspI AAT'ATT Cuts at: 5 52 3475 4129 5 Size: 47 3423 654 2081 Fragments arranged by size: 3423 2081 654 47 StuI AGG'CCT Cuts at: 1517 3118 1517 Size: 1601 4604 StyI C'CwwG_G Cuts at: 513 1065 2004 2484 2656 2667 3644 513 Size: 552 939 480 172 11 977 3074 Fragments arranged by size: 3074 977 939 552 480 172 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3386 4232 4249 4402 4587 5032 325 Size: 1255 1806 846 17 153 185 445 1498 Fragments arranged by size: 1806 1498 1255 846 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4451 6163 96 Size: 274 80 33 51 1443 2474 1712 138 Fragments arranged by size: 2474 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6165 6197 6165 Size: 32 6173 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6205 XbaI T'CTAG_A Cuts at: 3541 3541 Size: 6205 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2897 2897 Size: 6205 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6205 XmaI C'CCGG_G Cuts at: 1870 3530 1870 Size: 1660 4545 XmnI GAAnn'nnTTC Cuts at: 4334 4334 Size: 6205 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4013 276 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI