################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvrII BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BsaBI BsaI BsiEI BsiHKAI Bsp1407I BspDI BspHI BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI Ksp632I KspI MamI MfeI MluI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccII 12 CGCG CG^CG AccIII 489 TCCGGA T^CCGGA AflIII 1076 ACATGT A^C[GA][CT]GT Asp700I 1320 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 971 TGCGCA TGC^GCA BalI 119 TGGCCA TGG^CCA BanII 274 GGGCTC G[GA]GC[CT]^C BbeI 1357 GGCGCC GGCGC^C BbsI 696 GTCTTC GAAGAC(2/6) BlpI 1149 GCTTAGC GC^T[ACGT]AGC Bpu1102I 1149 GCTTAGC GC^T[ACGT]AGC BpuAI 696 GTCTTC GAAGAC(2/6) BsaAI 1030 TACGTG [CT]AC^GT[GA] BsaWI 489 TCCGGA [AT]^CCGG[AT] BseAI 489 TCCGGA T^CCGGA BsiWI 1028 CGTACG C^GTACG BsmAI 1112 GTCTC GTCTC(1/5) BsmBI 1111 CGTCTC CGTCTC(1/5) BsmI 760 GAATGC GAATGC(1/-1) BspEI 489 TCCGGA T^CCGGA BspLU11I 1076 ACATGT A^CATGT BsrDI 611 GCAATG GCAATG(2/0) BstUI 12 CGCG CG^CG BstXI 306 CCAGCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1149 GCTTAGC GC^T[ACGT]AGC DrdI 433 GACACTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 669 CCATGG C^C[GA][CT]GG EcoT14I 669 CCATGG C^C[AT][AT]GG FspI 971 TGCGCA TGC^GCA HgaI 64 GCGTC GACGC(5/10) KasI 1357 GGCGCC G^GCGCC MaeII 1031 ACGT A^CGT MluNI 119 TGGCCA TGG^CCA MroI 489 TCCGGA T^CCGGA MscI 119 TGGCCA TGG^CCA MslI 879 CACTGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 12 CGCG CG^CG NarI 1357 GGCGCC GG^CGCC NcoI 669 CCATGG C^CATGG NspI 1076 ACATGT [GA]CATG^[CT] PpuMI 249 GGGTCCC [GA]G^G[AT]CC[CT] PstI 258 CTGCAG CTGCA^G ScaI 143 AGTACT AGT^ACT SfcI 258 CTGCAG C^T[GA][CT]AG SfoI 1357 GGCGCC GGC^GCC SmaI 112 CCCGGG CCC^GGG SmlI 925 CTCAAG C^T[CT][GA]AG StyI 669 CCATGG C^C[AT][AT]GG XmaCI 112 CCCGGG C^CCGGG XmaI 112 CCCGGG C^CCGGG XmnI 1320 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccI 652 GTCTAC GT^[AC][GT]AC 1192 GTAGAC AcyI 63 GGCGTC G[GA]^CG[CT]C 1357 GGCGCC BamHI 210 GGATCC G^GATCC 576 GGATCC BanI 849 GGTGCC G^G[CT][GA]CC 1357 GGCGCC BfaI 52 CTAG C^TAG 268 CTAG BmyI 274 GGGCTC G[AGT]GC[ACT]^C 850 GTGCCC BsaHI 63 GGCGTC G[GA]^CG[CT]C 1357 GGCGCC BseRI 277 CTCCTC GAGGAG(10/8) 621 CTCCTC BsgI 987 CTGCAC GTGCAG(16/14) 1258 GTGCAG Bsp1286I 274 GGGCTC G[AGT]GC[ACT]^C 850 GTGCCC Hin1I 63 GGCGTC G[GA]^CG[CT]C 1357 GGCGCC HphI 320 TCACC GGTGA(8/7) 1400 TCACC MaeI 52 CTAG C^TAG 268 CTAG PflMI 891 CCAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1245 CCAGAAGATGG TfiI 348 GAATC G^A[AT]TC 1234 GATTC Tsp45I 319 GTCAC ^GT[GC]AC 766 GTGAC Tsp509I 454 AATT ^AATT 1207 AATT Van91I 891 CCAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1245 CCAGAAGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaI AvaII BbvI BcnI BpmI BsaJI BsiYI BslI BsmFI BsoBI BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI TseI TspRI XhoII