(Circular) (Six-base) MAPSORT of: pFN21AE4462.seq Check: 915 from: 1 to: 6142 pFN21AE4462 6142 bp With 182 enzymes: SgfI * March 28, 2012 14:03 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3952 278 Size: 53 83 186 936 2416 2468 Fragments arranged by size: 2468 2416 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2658 3198 3485 1937 Size: 721 540 287 4594 Fragments arranged by size: 4594 721 540 287 Acc65I G'GTAC_C Cuts at: 3463 3463 Size: 6142 AclI AA'CG_TT Cuts at: 1563 4269 4642 1563 Size: 2706 373 3063 Fragments arranged by size: 3063 2706 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2582 2765 3167 4205 5589 5857 1632 Size: 950 183 402 1038 1384 268 1917 Fragments arranged by size: 1917 1384 1038 950 402 268 183 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5945 AflIII A'CryG_T Cuts at: 1203 3081 5041 6018 1203 Size: 1878 1960 977 1327 Fragments arranged by size: 1960 1878 1327 977 AgeI A'CCGG_T Cuts at: 4973 4973 Size: 6142 AhdI GACnn_n'nnGTC Cuts at: 4871 4871 Size: 6142 AleI CACnn'nnGTG Cuts at: 1166 2888 1166 Size: 1722 4420 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6142 AlwNI CAG_nnn'CTG Cuts at: 2605 2953 3145 3885 5457 2605 Size: 348 192 740 1572 3290 Fragments arranged by size: 3290 1572 740 348 192 ApaLI G'TGCA_C Cuts at: 4199 5355 4199 Size: 1156 4986 ApoI r'AATT_y Cuts at: 1493 3451 3677 3829 5938 1493 Size: 1958 226 152 2109 1697 Fragments arranged by size: 2109 1958 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4696 160 Size: 872 3664 1606 Fragments arranged by size: 3664 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 AvaI C'yCGr_G Cuts at: 1870 1943 2073 2117 2219 3467 1870 Size: 73 130 44 102 1248 4545 Fragments arranged by size: 4545 1248 130 102 73 44 BamHI G'GATC_C Cuts at: 2215 2581 3472 3839 2215 Size: 366 891 367 4518 Fragments arranged by size: 4518 891 367 366 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2854 3362 3463 4918 618 Size: 325 217 627 1067 508 101 1455 1842 Fragments arranged by size: 1842 1455 1067 627 508 325 217 101 BanII G_rGCy'C Cuts at: 1391 2283 3461 1391 Size: 892 1178 4072 Fragments arranged by size: 4072 1178 892 BbeI G_GCGC'C Cuts at: 3366 3366 Size: 6142 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2694 928 Size: 971 795 4376 Fragments arranged by size: 4376 971 795 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4333 4367 1903 Size: 34 2396 34 3678 Fragments arranged by size: 3678 2396 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4034 5244 4034 Size: 1210 4932 BclI T'GATC_A Cuts at: 1548 1548 Size: 6142 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4753 136 Size: 107 122 71 631 605 3081 1525 Fragments arranged by size: 3081 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6137 6137 Size: 6142 BlpI GC'TnA_GC Cuts at: 3155 3559 3155 Size: 404 5738 Bme1580I G_kGCm'C Cuts at: 1790 2859 4203 5359 1790 Size: 1069 1344 1156 2573 Fragments arranged by size: 2573 1344 1156 1069 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6142 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3133 3873 4831 Size: 416 53 83 399 24 1716 740 958 Cuts at: 4831 442 Size: 1753 Fragments arranged by size: 1753 1716 958 740 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6142 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2351 2399 2693 2868 4802 1239 Size: 1112 48 294 175 1934 2579 Fragments arranged by size: 2579 1934 1112 294 175 48 Bpu10I CC'TnA_GC Cuts at: 2962 2962 Size: 6142 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2915 4267 5132 5430 2915 Size: 1352 865 298 3627 Fragments arranged by size: 3627 1352 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4805 882 Size: 617 3306 2219 Fragments arranged by size: 3306 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3037 3933 493 Size: 711 1833 896 2702 Fragments arranged by size: 2702 1833 896 711 BsaBI GATnn'nnATC Cuts at: 6136 6136 Size: 6142 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2069 3363 3949 4331 Size: 53 83 186 936 536 1294 586 382 Cuts at: 4331 4966 275 Size: 635 1451 Fragments arranged by size: 1451 1294 936 635 586 536 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2494 4575 4973 5247 5394 5805 2494 Size: 2081 398 274 147 411 2831 Fragments arranged by size: 2831 2081 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5917 Fragments arranged by size: 5917 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2273 2617 1186 Size: 1087 344 4711 Fragments arranged by size: 4711 1087 344 BseYI C'CCAG_C Cuts at: 2386 2390 2585 2858 5345 5841 2386 Size: 4 195 273 2487 496 2687 Fragments arranged by size: 2687 2487 496 273 195 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2977 3284 1163 Size: 573 1241 307 4021 Fragments arranged by size: 4021 1241 573 307 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3592 4353 5381 1540 Size: 275 186 1591 761 1028 2301 Fragments arranged by size: 2301 1591 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4203 4288 5359 4203 Size: 85 1071 4986 Fragments arranged by size: 4986 1071 85 BsiWI C'GTAC_G Cuts at: 3033 3033 Size: 6142 BsmI GAATG_Cn' Cuts at: 2771 3653 3746 2771 Size: 882 93 5167 Fragments arranged by size: 5167 882 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3122 3122 Size: 6142 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2283 2859 3461 4203 4288 5359 1391 Size: 399 493 576 602 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 602 576 493 399 85 BspEI T'CCGG_A Cuts at: 2494 5805 2494 Size: 3311 2831 BspHI T'CATG_A Cuts at: 4031 5761 4031 Size: 1730 4412 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3497 844 Size: 2653 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4029 1394 Size: 429 2206 3507 Fragments arranged by size: 3507 2206 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2623 4637 4811 65 Size: 1183 1375 2014 174 1396 Fragments arranged by size: 2014 1396 1375 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3270 3314 3365 4786 4973 1700 Size: 110 1460 44 51 1421 187 2869 Fragments arranged by size: 2869 1460 1421 187 110 51 44 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6142 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6142 BssSI C'ACGA_G Cuts at: 4202 5214 4202 Size: 1012 5130 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6142 BstBI TT'CG_AA Cuts at: 4946 4946 Size: 6142 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6142 BstXI CCAn_nnnn'nTGG Cuts at: 2318 2318 Size: 6142 BstYI r'GATC_y Cuts at: 1972 2103 2215 2581 3174 3472 3839 4224 4241 Size: 131 112 366 593 298 367 385 17 Cuts at: 4241 5693 5779 5791 6137 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 593 385 367 366 346 298 131 112 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2674 513 Size: 552 1609 3981 Fragments arranged by size: 3981 1609 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3325 487 Size: 683 56 2099 3304 Fragments arranged by size: 3304 2099 683 56 BtsI GCAGTG_nn' Cuts at: 2716 2951 3658 4452 4472 2716 Size: 235 707 794 20 4386 Fragments arranged by size: 4386 794 707 235 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6142 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6107 DraI TTT'AAA Cuts at: 3446 3446 Size: 6142 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2444 5149 809 Size: 507 1128 2705 1802 Fragments arranged by size: 2705 1802 1128 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2124 2562 3367 Size: 54 1425 50 275 18 294 438 805 Cuts at: 3367 3589 4478 6107 8 Size: 222 889 1629 43 Fragments arranged by size: 1629 1425 889 805 438 294 275 222 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3589 1537 Size: 275 1777 4090 Fragments arranged by size: 4090 1777 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4072 1353 Size: 2719 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2132 2474 4729 5103 5249 387 Size: 1064 138 543 342 2255 374 146 1280 Fragments arranged by size: 2255 1280 1064 543 374 342 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2351 2399 2582 2693 2765 2868 3167 Size: 393 719 48 183 111 72 103 299 Cuts at: 3167 4205 4802 5589 5857 1239 Size: 1038 597 787 268 1524 Fragments arranged by size: 1524 1038 787 719 597 393 299 268 183 111 103 72 48 EcoO109I rG'GnC_Cy Cuts at: 2255 2467 3046 2255 Size: 212 579 5351 Fragments arranged by size: 5351 579 212 EcoRI G'AATT_C Cuts at: 3451 3829 3451 Size: 378 5764 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2758 2758 Size: 6142 FspI TGC'GCA Cuts at: 2978 4648 2978 Size: 1670 4472 FspAI rTGC'GCAy Cuts at: 2978 2978 Size: 6142 HaeII r_GCGC'y Cuts at: 1678 2113 3271 3366 5289 1678 Size: 435 1158 95 1923 2531 Fragments arranged by size: 2531 1923 1158 435 95 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2929 2961 4783 4815 4857 Size: 32 135 32 1357 32 1822 32 42 Cuts at: 4857 4889 1373 Size: 32 2626 Fragments arranged by size: 2626 1822 1357 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3486 3732 669 Size: 988 281 1548 246 3079 Fragments arranged by size: 3079 1548 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6142 HpaI GTT'AAC Cuts at: 3732 3732 Size: 6142 KasI G'GCGC_C Cuts at: 3362 3362 Size: 6142 KpnI G_GTAC'C Cuts at: 3467 3467 Size: 6142 MfeI C'AATT_G Cuts at: 3741 3741 Size: 6142 MluI A'CGCG_T Cuts at: 6018 6018 Size: 6142 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5256 5440 1305 Size: 3951 184 2007 Fragments arranged by size: 3951 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2126 10 Size: 54 1425 343 294 4026 Fragments arranged by size: 4026 1425 343 294 54 MslI CAynn'nnrTG Cuts at: 518 1166 2888 4100 4459 4618 518 Size: 648 1722 1212 359 159 2042 Fragments arranged by size: 2042 1722 1212 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2029 2390 3008 3067 3300 3558 4235 5383 Size: 423 361 618 59 233 258 677 1148 Cuts at: 5383 5628 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 618 423 361 258 245 233 59 NaeI GCC'GGC Cuts at: 3272 3316 3367 3272 Size: 44 51 6047 Fragments arranged by size: 6047 51 44 NarI GG'CG_CC Cuts at: 3363 3363 Size: 6142 NcoI C'CATG_G Cuts at: 513 1065 2674 513 Size: 552 1609 3981 Fragments arranged by size: 3981 1609 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6142 NgoMIV G'CCGG_C Cuts at: 3270 3314 3365 3270 Size: 44 51 6047 Fragments arranged by size: 6047 51 44 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6142 NotI GC'GGCC_GC Cuts at: 3589 3589 Size: 6142 NspI r_CATG'y Cuts at: 1132 1229 3085 3500 5045 1132 Size: 97 1856 415 1545 2229 Fragments arranged by size: 2229 1856 1545 415 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6142 PciI A'CATG_T Cuts at: 3081 5041 3081 Size: 1960 4182 PflMI CCAn_nnn'nTGG Cuts at: 1108 2902 3256 1108 Size: 1794 354 3994 Fragments arranged by size: 3994 1794 354 PfoI T'CCnGG_A Cuts at: 2895 3057 2895 Size: 162 5980 PmeI GTTT'AAAC Cuts at: 3446 3446 Size: 6142 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4211 4243 5724 5756 4211 Size: 32 1481 32 4597 Fragments arranged by size: 4597 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2255 2255 Size: 6142 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6142 PsiI TTA'TAA Cuts at: 3712 3712 Size: 6142 PstI C_TGCA'G Cuts at: 830 2267 3494 830 Size: 1437 1227 3478 Fragments arranged by size: 3478 1437 1227 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6142 PvuII CAG'CTG Cuts at: 2390 3008 3067 2390 Size: 618 59 5465 Fragments arranged by size: 5465 618 59 SalI G'TCGA_C Cuts at: 1936 3484 1936 Size: 1548 4594 SanDI GG'GwC_CC Cuts at: 2255 2255 Size: 6142 SbfI CC_TGCA'GG Cuts at: 3494 3494 Size: 6142 ScaI AGT'ACT Cuts at: 2150 4390 6102 2150 Size: 2240 1712 2190 Fragments arranged by size: 2240 2190 1712 SfcI C'TryA_G Cuts at: 826 1045 2263 3490 4625 5306 5497 826 Size: 219 1218 1227 1135 681 191 1471 Fragments arranged by size: 1471 1227 1218 1135 681 219 191 SfoI GGC'GCC Cuts at: 3364 3364 Size: 6142 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6142 SmaI CCC'GGG Cuts at: 1872 2119 3469 1872 Size: 247 1350 4545 Fragments arranged by size: 4545 1350 247 SmlI C'TyrA_G Cuts at: 820 1017 1943 2930 4246 5147 5409 820 Size: 197 926 987 1316 901 262 1553 Fragments arranged by size: 1553 1316 987 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3933 493 Size: 3440 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6142 SphI G_CATG'C Cuts at: 1132 3500 1132 Size: 2368 3774 SspI AAT'ATT Cuts at: 5 52 4066 5 Size: 47 4014 2081 Fragments arranged by size: 4014 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6142 StyI C'CwwG_G Cuts at: 513 1065 2674 3581 513 Size: 552 1609 907 3074 Fragments arranged by size: 3074 1609 907 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4169 4186 4339 4524 4969 325 Size: 1255 2589 17 153 185 445 1498 Fragments arranged by size: 2589 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2148 2334 2499 Size: 274 80 33 51 1443 171 186 165 Cuts at: 2499 4388 6100 96 Size: 1889 1712 138 Fragments arranged by size: 1889 1712 1443 274 186 171 165 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6102 6134 6102 Size: 32 6110 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6142 XbaI T'CTAG_A Cuts at: 3478 3478 Size: 6142 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6142 XmaI C'CCGG_G Cuts at: 1870 2117 3467 1870 Size: 247 1350 4545 Fragments arranged by size: 4545 1350 247 XmnI GAAnn'nnTTC Cuts at: 3329 4271 3329 Size: 942 5200 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3950 276 Size: 53 83 186 936 2416 2468 Fragments arranged by size: 2468 2416 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI XcmI