(Circular) (Six-base) MAPSORT of: pFN21AB3480.seq Check: 2783 from: 1 to: 5653 pFN21AB3480 5653 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2794 3463 278 Size: 53 83 186 936 1258 669 2468 Fragments arranged by size: 2468 1258 936 669 186 83 53 AccI GT'mk_AC Cuts at: 1937 2996 1937 Size: 1059 4594 Acc65I G'GTAC_C Cuts at: 2974 2974 Size: 5653 AclI AA'CG_TT Cuts at: 1563 2952 3780 4153 1563 Size: 1389 828 373 3063 Fragments arranged by size: 3063 1389 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3716 5100 5368 1632 Size: 2084 1384 268 1917 Fragments arranged by size: 2084 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5456 AflIII A'CryG_T Cuts at: 1203 2307 4552 5529 1203 Size: 1104 2245 977 1327 Fragments arranged by size: 2245 1327 1104 977 AgeI A'CCGG_T Cuts at: 4484 4484 Size: 5653 AhdI GACnn_n'nnGTC Cuts at: 4382 4382 Size: 5653 AleI CACnn'nnGTG Cuts at: 1166 2547 1166 Size: 1381 4272 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5653 AlwNI CAG_nnn'CTG Cuts at: 3396 4968 3396 Size: 1572 4081 ApaLI G'TGCA_C Cuts at: 3710 4866 3710 Size: 1156 4497 ApoI r'AATT_y Cuts at: 1493 2492 2962 3188 3340 5449 1493 Size: 999 470 226 152 2109 1697 Fragments arranged by size: 2109 1697 999 470 226 152 AseI AT'TA_AT Cuts at: 160 1032 4207 160 Size: 872 3175 1606 Fragments arranged by size: 3175 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 AvaI C'yCGr_G Cuts at: 1870 1943 2978 1870 Size: 73 1035 4545 Fragments arranged by size: 4545 1035 73 BamHI G'GATC_C Cuts at: 2506 2983 3350 2506 Size: 477 367 4809 Fragments arranged by size: 4809 477 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2427 2884 2974 4429 618 Size: 325 217 627 640 457 90 1455 1842 Fragments arranged by size: 1842 1455 640 627 457 325 217 90 BanII G_rGCy'C Cuts at: 1391 2142 2197 2972 1391 Size: 751 55 775 4072 Fragments arranged by size: 4072 775 751 55 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4682 BbvCI CC'TCA_GC Cuts at: 2333 2423 2333 Size: 90 5563 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3844 3878 1903 Size: 34 1907 34 3678 Fragments arranged by size: 3678 1907 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3545 4755 3545 Size: 1210 4443 BclI T'GATC_A Cuts at: 1548 1548 Size: 5653 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2319 4264 136 Size: 107 122 71 631 605 647 1945 1525 Fragments arranged by size: 1945 1525 647 631 605 122 107 71 BglII A'GATC_T Cuts at: 5648 5648 Size: 5653 BlpI GC'TnA_GC Cuts at: 3070 3070 Size: 5653 Bme1580I G_kGCm'C Cuts at: 1790 2625 2887 3714 4870 1790 Size: 835 262 827 1156 2573 Fragments arranged by size: 2573 1156 835 827 262 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5653 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3384 4342 442 Size: 416 53 83 399 24 1967 958 1753 Fragments arranged by size: 1967 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5653 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2230 2230 Size: 5653 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2144 2363 2414 4313 1239 Size: 905 219 51 1899 2579 Fragments arranged by size: 2579 1899 905 219 51 Bpu10I CC'TnA_GC Cuts at: 2333 2423 2333 Size: 90 5563 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2156 3778 4643 4941 2156 Size: 1622 865 298 2868 Fragments arranged by size: 2868 1622 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2009 4316 882 Size: 617 510 2307 2219 Fragments arranged by size: 2307 2219 617 510 BsaAI yAC'GTr Cuts at: 493 1204 2310 3444 493 Size: 711 1106 1134 2702 Fragments arranged by size: 2702 1134 1106 711 BsaBI GATnn'nnATC Cuts at: 2804 5647 2804 Size: 2843 2810 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2791 3460 3842 4477 Size: 53 83 186 936 1258 669 382 635 Cuts at: 4477 275 Size: 1451 Fragments arranged by size: 1451 1258 936 669 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4086 4484 4758 4905 5316 4086 Size: 398 274 147 411 4423 Fragments arranged by size: 4423 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2294 2324 2908 2938 1178 Size: 30 165 30 891 30 584 30 3893 Fragments arranged by size: 3893 891 584 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5653 BseYI C'CCAG_C Cuts at: 2264 2624 4856 5352 2264 Size: 360 2232 496 2565 Fragments arranged by size: 2565 2232 496 360 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2545 1163 Size: 573 809 4271 Fragments arranged by size: 4271 809 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3103 3864 4892 1540 Size: 275 186 1102 761 1028 2301 Fragments arranged by size: 2301 1102 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2160 2379 3714 3799 4870 2160 Size: 219 1335 85 1071 2943 Fragments arranged by size: 2943 1335 1071 219 85 BsmI GAATG_Cn' Cuts at: 3164 3257 3164 Size: 93 5560 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2142 2160 2197 2379 2625 2887 2972 Size: 399 352 18 37 182 246 262 85 Cuts at: 2972 3714 3799 4870 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 352 262 246 182 85 85 37 18 BspEI T'CCGG_A Cuts at: 5316 5316 Size: 5653 BspHI T'CATG_A Cuts at: 3542 5272 3542 Size: 1730 3923 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3008 844 Size: 2164 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3540 1394 Size: 429 1717 3507 Fragments arranged by size: 3507 1717 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2670 4148 4322 65 Size: 1183 1422 1478 174 1396 Fragments arranged by size: 1478 1422 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4297 4484 1700 Size: 110 2487 187 2869 Fragments arranged by size: 2869 2487 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5653 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5653 BssSI C'ACGA_G Cuts at: 3713 4725 3713 Size: 1012 4641 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5653 BstBI TT'CG_AA Cuts at: 4457 4457 Size: 5653 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5653 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5653 BstYI r'GATC_y Cuts at: 1972 2506 2983 3350 3735 3752 5204 5290 5302 Size: 534 477 367 385 17 1452 86 12 Cuts at: 5302 5648 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 534 477 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2144 513 Size: 552 1079 4022 Fragments arranged by size: 4022 1079 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4914 Fragments arranged by size: 4914 683 56 BtsI GCAGTG_nn' Cuts at: 2557 3169 3963 3983 2557 Size: 612 794 20 4227 Fragments arranged by size: 4227 794 612 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5653 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5618 DraI TTT'AAA Cuts at: 2957 2957 Size: 5653 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2023 4660 809 Size: 507 707 2637 1802 Fragments arranged by size: 2637 1802 707 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2320 3100 3989 Size: 54 1425 50 275 18 490 780 889 Cuts at: 3989 5618 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 780 490 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3100 1537 Size: 275 1288 4090 Fragments arranged by size: 4090 1288 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2644 2925 3583 1353 Size: 1291 281 658 3423 Fragments arranged by size: 3423 1291 658 281 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4240 4614 4760 387 Size: 1064 138 2651 374 146 1280 Fragments arranged by size: 2651 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 2363 2414 3716 4313 5100 5368 Size: 393 512 219 51 1302 597 787 268 Cuts at: 5368 1239 Size: 1524 Fragments arranged by size: 1524 1302 787 597 512 393 268 219 51 EcoRI G'AATT_C Cuts at: 2962 3340 2962 Size: 378 5275 FspI TGC'GCA Cuts at: 4159 4159 Size: 5653 HaeII r_GCGC'y Cuts at: 1678 4800 1678 Size: 3122 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2400 2432 2908 2940 4294 Size: 32 135 32 828 32 476 32 1354 Cuts at: 4294 4326 4368 4400 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1354 828 476 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2941 2997 3243 669 Size: 988 281 1003 56 246 3079 Fragments arranged by size: 3079 1003 988 281 246 56 HindIII A'AGCT_T Cuts at: 748 748 Size: 5653 HpaI GTT'AAC Cuts at: 3243 3243 Size: 5653 KpnI G_GTAC'C Cuts at: 2978 2978 Size: 5653 MfeI C'AATT_G Cuts at: 3252 3252 Size: 5653 MluI A'CGCG_T Cuts at: 5529 5529 Size: 5653 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4767 4951 1305 Size: 3462 184 2007 Fragments arranged by size: 3462 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2322 10 Size: 54 1425 343 490 3341 Fragments arranged by size: 3341 1425 490 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2547 3611 3970 4129 518 Size: 648 843 538 1064 359 159 2042 Fragments arranged by size: 2042 1064 843 648 538 359 159 MspA1I CmG'CkG Cuts at: 1606 2298 2436 2852 3069 3746 4894 5139 1606 Size: 692 138 416 217 677 1148 245 2120 Fragments arranged by size: 2120 1148 692 677 416 245 217 138 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5101 NdeI CA'TA_TG Cuts at: 387 387 Size: 5653 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5653 NotI GC'GGCC_GC Cuts at: 3100 3100 Size: 5653 NspI r_CATG'y Cuts at: 1132 1229 2187 3011 4556 1132 Size: 97 958 824 1545 2229 Fragments arranged by size: 2229 1545 958 824 97 PasI CC'CwG_GG Cuts at: 1348 2880 1348 Size: 1532 4121 PciI A'CATG_T Cuts at: 4552 4552 Size: 5653 PflMI CCAn_nnn'nTGG Cuts at: 1108 2223 1108 Size: 1115 4538 PfoI T'CCnGG_A Cuts at: 2160 2400 2160 Size: 240 5413 PmeI GTTT'AAAC Cuts at: 2957 2957 Size: 5653 PmlI CAC'GTG Cuts at: 2310 2310 Size: 5653 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3722 3754 5235 5267 3722 Size: 32 1481 32 4108 Fragments arranged by size: 4108 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5653 PsiI TTA'TAA Cuts at: 3223 3223 Size: 5653 PstI C_TGCA'G Cuts at: 830 2098 2726 3005 830 Size: 1268 628 279 3478 Fragments arranged by size: 3478 1268 628 279 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5653 PvuII CAG'CTG Cuts at: 2436 2852 2436 Size: 416 5237 SalI G'TCGA_C Cuts at: 1936 2995 1936 Size: 1059 4594 SapI GCTCTTCn'nnn_ Cuts at: 2644 2644 Size: 5653 SbfI CC_TGCA'GG Cuts at: 3005 3005 Size: 5653 ScaI AGT'ACT Cuts at: 3901 5613 3901 Size: 1712 3941 SfcI C'TryA_G Cuts at: 826 1045 2094 2269 2682 2703 2722 3001 4136 Size: 219 1049 175 413 21 19 279 1135 Cuts at: 4136 4817 5008 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1049 681 413 279 219 191 175 21 19 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5653 SmaI CCC'GGG Cuts at: 1872 2980 1872 Size: 1108 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2135 3757 4658 4920 820 Size: 197 926 192 1622 901 262 1553 Fragments arranged by size: 1622 1553 926 901 262 197 192 SnaBI TAC'GTA Cuts at: 493 3444 493 Size: 2951 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5653 SphI G_CATG'C Cuts at: 1132 3011 1132 Size: 1879 3774 SspI AAT'ATT Cuts at: 5 52 3577 5 Size: 47 3525 2081 Fragments arranged by size: 3525 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5653 StyI C'CwwG_G Cuts at: 513 1065 3092 513 Size: 552 2027 3074 Fragments arranged by size: 3074 2027 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2267 3680 3697 3850 4035 4480 325 Size: 1255 687 1413 17 153 185 445 1498 Fragments arranged by size: 1498 1413 1255 687 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2304 3899 5611 Size: 274 80 33 51 1443 327 1595 1712 Cuts at: 5611 96 Size: 138 Fragments arranged by size: 1712 1595 1443 327 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2294 2326 5613 5645 2294 Size: 32 3287 32 2302 Fragments arranged by size: 3287 2302 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2936 1316 Size: 1620 4033 XbaI T'CTAG_A Cuts at: 2989 2989 Size: 5653 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2543 2543 Size: 5653 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5653 XmaI C'CCGG_G Cuts at: 1870 2978 1870 Size: 1108 4545 XmnI GAAnn'nnTTC Cuts at: 3782 3782 Size: 5653 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2792 3461 276 Size: 53 83 186 936 1258 669 2468 Fragments arranged by size: 2468 1258 936 669 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI