################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT14I EcoT22I FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AciI 7 GCGG CCGC(-3/-1) AseI 553 ATTAAT AT^TAAT AsnI 553 ATTAAT AT^TAAT AspHI 943 GAGCAC G[AT]GC[AT]^C AvaII 30 GGTCC G^G[AT]CC BbsI 751 GTCTTC GAAGAC(2/6) BclI 414 TGATCA T^GATCA BmyI 943 GAGCAC G[AGT]GC[ACT]^C BpuAI 751 GTCTTC GAAGAC(2/6) BsaBI 622 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 451 GAGGAG GAGGAG(10/8) BsgI 1117 GTGCAG GTGCAG(16/14) BsiHKAI 943 GAGCAC G[AT]GC[AT]^C BsmFI 223 GTCCC GGGAC(10/14) Bsp1286I 943 GAGCAC G[AGT]GC[ACT]^C Bsp1407I 346 TGTACA T^GTACA BsrGI 346 TGTACA T^GTACA BstBI 754 TTCGAA TT^CGAA BstYI 627 AGATCC [GA]^GATC[CT] DraII 29 GGGTCCT [GA]G^G[ACGT]CC[CT] EarI 236 GAAGAG CTCTTC(1/4) EcoNI 487 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 29 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoRI 689 GAATTC G^AATTC FauI 7 GCGGG CCCGC(4/6) FbaI 414 TGATCA T^GATCA HaeIII 721 GGCC GG^CC Ksp632I 236 GAAGAG CTCTTC(1/4) MamI 622 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 627 AGATCC [GA]^GATC[CT] MspA1I 5 CAGCGG C[AC]G^C[GT]G NlaIII 717 CATG CATG^ NspV 754 TTCGAA TT^CGAA PpuMI 29 GGGTCCT [GA]G^G[AT]CC[CT] PshBI 553 ATTAAT AT^TAAT SapI 236 GAAGAGC GCTCTTC(1/4) SfuI 754 TTCGAA TT^CGAA SspBI 346 TGTACA T^GTACA Tsp45I 794 GTGAC ^GT[GC]AC TspRI 185 CAGTG CA[GC]TG[ACGT][ACGT]^ XcmI 899 CCAAGTATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 627 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AlwI 622 GATCC GGATC(4/5) 628 GATCC Asp700I 38 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 918 GAAAACTTTC BpmI 488 CTGGAG CTGGAG(16/14) 1082 CTGGAG Cfr13I 30 GGTCC G^G[ACGT]CC 720 GGGCC DdeI 34 CTGAG C^T[ACGT]AG 449 CTGAG HinfI 662 GATTC G^A[ACGT]TC 1033 GATTC MaeII 90 ACGT A^CGT 615 ACGT MslI 180 CATCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 458 CATTGATGTG NlaIV 29 GGGTCC GG[ACGT]^[ACGT]CC 453 GGAGCC PstI 24 CTGCAG CTGCA^G 191 CTGCAG Sau96I 30 GGTCC G^G[ACGT]CC 720 GGGCC TfiI 662 GATTC G^A[AT]TC 1033 GATTC XmnI 38 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 918 GAAAACTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AcsI AfaI AluI AlwNI ApoI BbvI BfaI BsaJI BsiYI BslI BsrDI BsrI BssKI BstF5I BstNI Cac8I DpnII EcoRII Fnu4HI FokI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII RsaI Sau3AI ScrFI SfaNI SfcI TaqI Tru9I TseI Tsp509I TthHB8I