(Circular) (Six-base) MAPSORT of: pFN21AE4406.seq Check: 8155 from: 1 to: 5968 pFN21AE4406 5968 bp With 182 enzymes: SgfI * March 28, 2012 13:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3778 278 Size: 53 83 186 936 2242 2468 Fragments arranged by size: 2468 2242 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3311 1937 Size: 1374 4594 Acc65I G'GTAC_C Cuts at: 3289 3289 Size: 5968 AclI AA'CG_TT Cuts at: 1563 4095 4468 1563 Size: 2532 373 3063 Fragments arranged by size: 3063 2532 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4031 5415 5683 1632 Size: 2399 1384 268 1917 Fragments arranged by size: 2399 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5771 AflIII A'CryG_T Cuts at: 1203 4867 5844 1203 Size: 3664 977 1327 Fragments arranged by size: 3664 1327 977 AgeI A'CCGG_T Cuts at: 4799 4799 Size: 5968 AhdI GACnn_n'nnGTC Cuts at: 4697 4697 Size: 5968 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5968 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5968 AlwNI CAG_nnn'CTG Cuts at: 2195 2492 2504 3711 5283 2195 Size: 297 12 1207 1572 2880 Fragments arranged by size: 2880 1572 1207 297 12 ApaLI G'TGCA_C Cuts at: 4025 5181 4025 Size: 1156 4812 ApoI r'AATT_y Cuts at: 1493 2144 2165 2380 2694 3219 3277 3503 3655 Size: 651 21 215 314 525 58 226 152 Cuts at: 3655 5764 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 651 525 314 226 215 152 58 21 AseI AT'TA_AT Cuts at: 160 1032 2559 4522 160 Size: 872 1527 1963 1606 Fragments arranged by size: 1963 1606 1527 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5968 AvaI C'yCGr_G Cuts at: 1870 1943 3293 1870 Size: 73 1350 4545 Fragments arranged by size: 4545 1350 73 BamHI G'GATC_C Cuts at: 3298 3665 3298 Size: 367 5601 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3289 4744 618 Size: 325 217 627 1502 1455 1842 Fragments arranged by size: 1842 1502 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3287 1391 Size: 1896 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2749 928 Size: 971 850 4147 Fragments arranged by size: 4147 971 850 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4159 4193 1903 Size: 34 2222 34 3678 Fragments arranged by size: 3678 2222 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3860 5070 3860 Size: 1210 4758 BclI T'GATC_A Cuts at: 1548 2419 1548 Size: 871 5097 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4579 136 Size: 107 122 71 631 605 2907 1525 Fragments arranged by size: 2907 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5963 5963 Size: 5968 BlpI GC'TnA_GC Cuts at: 3385 3385 Size: 5968 Bme1580I G_kGCm'C Cuts at: 1790 4029 5185 1790 Size: 2239 1156 2573 Fragments arranged by size: 2573 2239 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5968 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2183 3699 4657 Size: 416 53 83 399 24 766 1516 958 Cuts at: 4657 442 Size: 1753 Fragments arranged by size: 1753 1516 958 766 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5968 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2514 3108 4628 1239 Size: 1275 594 1520 2579 Fragments arranged by size: 2579 1520 1275 594 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4093 4958 5256 4093 Size: 865 298 4805 Fragments arranged by size: 4805 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4631 882 Size: 617 3132 2219 Fragments arranged by size: 3132 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3759 493 Size: 711 2555 2702 Fragments arranged by size: 2702 2555 711 BsaBI GATnn'nnATC Cuts at: 2631 5962 2631 Size: 3331 2637 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3775 4157 4792 275 Size: 53 83 186 936 2242 382 635 1451 Fragments arranged by size: 2242 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4401 4799 5073 5220 5631 4401 Size: 398 274 147 411 4738 Fragments arranged by size: 4738 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5743 Fragments arranged by size: 5743 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2471 1186 Size: 1285 4683 BseYI C'CCAG_C Cuts at: 5171 5667 5171 Size: 496 5472 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3143 1163 Size: 573 1407 3988 Fragments arranged by size: 3988 1407 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3418 4179 5207 1540 Size: 275 186 1417 761 1028 2301 Fragments arranged by size: 2301 1417 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2952 4029 4114 5185 2952 Size: 1077 85 1071 3735 Fragments arranged by size: 3735 1077 1071 85 BsmI GAATG_Cn' Cuts at: 3479 3572 3479 Size: 93 5875 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2952 3287 4029 4114 5185 1391 Size: 399 1162 335 742 85 1071 2174 Fragments arranged by size: 2174 1162 1071 742 399 335 85 BspEI T'CCGG_A Cuts at: 5631 5631 Size: 5968 BspHI T'CATG_A Cuts at: 3857 5587 3857 Size: 1730 4238 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3323 844 Size: 2479 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3855 1394 Size: 429 2032 3507 Fragments arranged by size: 3507 2032 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2829 2991 3145 4463 4637 65 Size: 1183 1581 162 154 1318 174 1396 Fragments arranged by size: 1581 1396 1318 1183 174 162 154 BsrFI r'CCGG_y Cuts at: 1700 1810 4612 4799 1700 Size: 110 2802 187 2869 Fragments arranged by size: 2869 2802 187 110 BsrGI T'GTAC_A Cuts at: 96 2351 96 Size: 2255 3713 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5968 BssSI C'ACGA_G Cuts at: 4028 5040 4028 Size: 1012 4956 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5968 BstBI TT'CG_AA Cuts at: 2760 4772 2760 Size: 2012 3956 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5968 BstYI r'GATC_y Cuts at: 1972 2632 3298 3665 4050 4067 5519 5605 5617 Size: 660 666 367 385 17 1452 86 12 Cuts at: 5617 5963 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 666 660 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4477 Fragments arranged by size: 4477 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2038 2746 487 Size: 683 56 812 708 3709 Fragments arranged by size: 3709 812 708 683 56 BtsI GCAGTG_nn' Cuts at: 3484 4278 4298 3484 Size: 794 20 5154 Fragments arranged by size: 5154 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5968 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5933 DraI TTT'AAA Cuts at: 3272 3272 Size: 5968 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4975 809 Size: 507 3659 1802 Fragments arranged by size: 3659 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3415 4304 5933 Size: 54 1425 50 275 18 1585 889 1629 Cuts at: 5933 8 Size: 43 Fragments arranged by size: 1629 1585 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3415 1537 Size: 275 1603 4090 Fragments arranged by size: 4090 1603 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2236 3898 1353 Size: 883 1662 3423 Fragments arranged by size: 3423 1662 883 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4555 4929 5075 387 Size: 1064 138 2966 374 146 1280 Fragments arranged by size: 2966 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2514 3108 4031 4628 5415 5683 1239 Size: 393 882 594 923 597 787 268 1524 Fragments arranged by size: 1524 923 882 787 597 594 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2496 2496 Size: 5968 EcoO109I rG'GnC_Cy Cuts at: 2035 2035 Size: 5968 EcoRI G'AATT_C Cuts at: 2694 3277 3655 2694 Size: 583 378 5007 Fragments arranged by size: 5007 583 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2943 2943 Size: 5968 FspI TGC'GCA Cuts at: 4474 4474 Size: 5968 HaeII r_GCGC'y Cuts at: 1678 5115 1678 Size: 3437 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2166 2198 2739 2771 4609 Size: 32 135 32 594 32 541 32 1838 Cuts at: 4609 4641 4683 4715 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1838 594 541 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3312 3558 669 Size: 988 281 1374 246 3079 Fragments arranged by size: 3079 1374 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5968 HpaI GTT'AAC Cuts at: 3558 3558 Size: 5968 KpnI G_GTAC'C Cuts at: 3293 3293 Size: 5968 MfeI C'AATT_G Cuts at: 3567 3567 Size: 5968 MluI A'CGCG_T Cuts at: 5844 5844 Size: 5968 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2851 5082 5266 1305 Size: 1546 2231 184 2007 Fragments arranged by size: 2231 2007 1546 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4146 Fragments arranged by size: 4146 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2189 2467 3926 4285 4444 518 Size: 648 1023 278 1459 359 159 2042 Fragments arranged by size: 2042 1459 1023 648 359 278 159 MspA1I CmG'CkG Cuts at: 1606 2012 3384 4061 5209 5454 1606 Size: 406 1372 677 1148 245 2120 Fragments arranged by size: 2120 1372 1148 677 406 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4477 Fragments arranged by size: 4477 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5968 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5968 NotI GC'GGCC_GC Cuts at: 3415 3415 Size: 5968 NspI r_CATG'y Cuts at: 1132 1229 3326 4871 1132 Size: 97 2097 1545 2229 Fragments arranged by size: 2229 2097 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5968 PciI A'CATG_T Cuts at: 4867 4867 Size: 5968 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5968 PmeI GTTT'AAAC Cuts at: 3272 3272 Size: 5968 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4037 4069 5550 5582 4037 Size: 32 1481 32 4423 Fragments arranged by size: 4423 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2035 2035 Size: 5968 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5968 PsiI TTA'TAA Cuts at: 3538 3538 Size: 5968 PstI C_TGCA'G Cuts at: 830 2033 2200 3320 830 Size: 1203 167 1120 3478 Fragments arranged by size: 3478 1203 1120 167 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5968 SalI G'TCGA_C Cuts at: 1936 3310 1936 Size: 1374 4594 SapI GCTCTTCn'nnn_ Cuts at: 2236 2236 Size: 5968 SbfI CC_TGCA'GG Cuts at: 3320 3320 Size: 5968 ScaI AGT'ACT Cuts at: 4216 5928 4216 Size: 1712 4256 SfcI C'TryA_G Cuts at: 826 1045 2029 2196 2850 3162 3316 4451 5132 Size: 219 984 167 654 312 154 1135 681 Cuts at: 5132 5323 826 Size: 191 1471 Fragments arranged by size: 1471 1135 984 681 654 312 219 191 167 154 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5968 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5968 SmaI CCC'GGG Cuts at: 1872 3295 1872 Size: 1423 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4072 4973 5235 820 Size: 197 926 2129 901 262 1553 Fragments arranged by size: 2129 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3759 493 Size: 3266 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5968 SphI G_CATG'C Cuts at: 1132 3326 1132 Size: 2194 3774 SspI AAT'ATT Cuts at: 5 52 3892 5 Size: 47 3840 2081 Fragments arranged by size: 3840 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5968 StyI C'CwwG_G Cuts at: 513 1065 2004 3407 513 Size: 552 939 1403 3074 Fragments arranged by size: 3074 1403 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3995 4012 4165 4350 4795 325 Size: 1255 2415 17 153 185 445 1498 Fragments arranged by size: 2415 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2351 3258 4214 Size: 274 80 33 51 1443 374 907 956 Cuts at: 4214 5926 96 Size: 1712 138 Fragments arranged by size: 1712 1443 956 907 374 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2260 2292 2658 2690 5928 5960 2260 Size: 32 366 32 3238 32 2268 Fragments arranged by size: 3238 2268 366 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5968 XbaI T'CTAG_A Cuts at: 3304 3304 Size: 5968 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2911 2911 Size: 5968 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5968 XmaI C'CCGG_G Cuts at: 1870 3293 1870 Size: 1423 4545 XmnI GAAnn'nnTTC Cuts at: 2047 2927 4097 2047 Size: 880 1170 3918 Fragments arranged by size: 3918 1170 880 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3776 276 Size: 53 83 186 936 2242 2468 Fragments arranged by size: 2468 2242 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI