################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII Acc65I AccI AccIII AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BclI BfrI BglI BlnI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmI BsoBI BspEI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Cfr10I CpoI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV FbaI FseI FspI HaeII HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI NaeI NarI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AcyI 209 GACGCC G[GA]^CG[CT]C AflIII 2668 ACATGT A^C[GA][CT]GT AocI 2281 CCTGAGG CC^T[ACGT]AGG AspHI 1838 GAGCAC G[AT]GC[AT]^C BbvCI 1384 GCTGAGG CCTCAGC(-5/-2) BlpI 1120 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1120 GCTGAGC GC^T[ACGT]AGC BsaAI 118 TACGTG [CT]AC^GT[GA] BsaBI 1210 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 209 GACGCC G[GA]^CG[CT]C BseRI 3152 GAGGAG GAGGAG(10/8) BsiHKAI 1838 GAGCAC G[AT]GC[AT]^C BsmBI 1737 GAGACG CGTCTC(1/5) Bsp1407I 918 TGTACA T^GTACA BspDI 371 ATCGAT AT^CGAT BspHI 753 TCATGA T^CATGA BspLU11I 2668 ACATGT A^CATGT BsrDI 80 GCAATG GCAATG(2/0) BsrGI 918 TGTACA T^GTACA Bsu36I 2281 CCTGAGG CC^T[ACGT]AGG CelII 1120 GCTGAGC GC^T[ACGT]AGC ClaI 371 ATCGAT AT^CGAT DraI 1440 TTTAAA TTT^AAA DsaI 2535 CCATGG C^C[GA][CT]GG Eco81I 2281 CCTGAGG CC^T[ACGT]AGG EcoNI 219 CCTCATCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 2036 ATGCAT ATGCA^T HgaI 209 GACGC GACGC(5/10) Hin1I 209 GACGCC G[GA]^CG[CT]C HindIII 2501 AAGCTT A^AGCTT MamI 1210 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 2535 CCATGG C^CATGG NheI 1033 GCTAGC G^CTAGC NsiI 2036 ATGCAT ATGCA^T RcaI 753 TCATGA T^CATGA SapI 2549 GAAGAGC GCTCTTC(1/4) SspBI 918 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AclI 569 AACGTT AA^CGTT 1526 AACGTT AlwI 374 GATCC GGATC(4/5) 768 GGATC BanI 70 GGCACC G^G[CT][GA]CC 161 GGCACC BanII 1153 GAGCCC G[GA]GC[CT]^C 2238 GGGCTC BcgI 54 CGAGCCGTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 167 GCAAGACCTTCG BciVI 523 GTATCC GTATCC(6/5) 3012 GTATCC BglII 720 AGATCT A^GATCT 2337 AGATCT BpmI 184 CTGGAG CTGGAG(16/14) 434 CTGGAG BsiYI 219 CCTCATCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 2565 CCTGGAACGGG BslI 219 CCTCATCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 2565 CCTGGAACGGG BstXI 554 CCAAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2043 CCAGGTCTGTGG DraII 215 GGGACCT [GA]G^G[ACGT]CC[CT] 240 GGGTCCC EcoO109I 215 GGGACCT [GA]G^G[ACGT]CC[CT] 240 GGGTCCC EcoT14I 2535 CCATGG C^C[AT][AT]GG 2868 CCAAGG FauI 131 GCGGG CCCGC(4/6) 1944 GCGGG HincII 547 GTTGAC GT[CT]^[GA]AC 1429 GTTGAC HindII 547 GTTGAC GT[CT]^[GA]AC 1429 GTTGAC HphI 2728 GGTGA GGTGA(8/7) 255 TCACC PpuMI 215 GGGACCT [GA]G^G[AT]CC[CT] 240 GGGTCCC Psp1406I 569 AACGTT AA^CGTT 1526 AACGTT PstI 196 CTGCAG CTGCA^G 1582 CTGCAG PvuII 607 CAGCTG CAG^CTG 1382 CAGCTG SphI 2038 GCATGC GCATG^C 2912 GCATGC StyI 2535 CCATGG C^C[AT][AT]GG 2868 CCAAGG Tsp45I 2452 GTCAC ^GT[GC]AC 2962 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwNI ApoI Asp700I AvaII BbsI BbvI BcnI BfaI BmyI BpuAI BsaJI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII XmnI