Restriction map for
pFN21AE4260
(Circular) (Six-base) MAPSORT of: pFN21AE4260.seq Check: 3137 from: 1 to: 7864
pFN21AE4260 7864 bp
With 182 enzymes: SgfI *
March 28, 2012 13:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5674 278
Size: 53 83 186 936 4138 2468
Fragments arranged by size:
4138 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5207 1937
Size: 3270 4594
Acc65I G'GTAC_C
Cuts at: 5185 5185
Size: 7864
AclI AA'CG_TT
Cuts at: 1563 2575 3532 5991 6364 1563
Size: 1012 957 2459 373 3063
Fragments arranged by size:
3063 2459 1012 957 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3156 4844 5031 5124 5927 7311 7579 1632
Size: 1524 1688 187 93 803 1384 268 1917
Fragments arranged by size:
1917 1688 1524 1384 803 268 187 93
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7667
AflIII A'CryG_T
Cuts at: 1203 4673 6763 7740 1203
Size: 3470 2090 977 1327
Fragments arranged by size:
3470 2090 1327 977
AgeI A'CCGG_T
Cuts at: 6695 6695
Size: 7864
AhdI GACnn_n'nnGTC
Cuts at: 6593 6593
Size: 7864
AleI CACnn'nnGTG
Cuts at: 1166 4026 1166
Size: 2860 5004
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4856 1184
Size: 3672 4192
AlwNI CAG_nnn'CTG
Cuts at: 3125 3179 5009 5607 7179 3125
Size: 54 1830 598 1572 3810
Fragments arranged by size:
3810 1830 1572 598 54
ApaLI G'TGCA_C
Cuts at: 5921 7077 5921
Size: 1156 6708
ApoI r'AATT_y
Cuts at: 1493 2816 3953 4493 4601 4808 5173 5399 5551
Size: 1323 1137 540 108 207 365 226 152
Cuts at: 5551 7660 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1323 1137 540 365 226 207
152 108
AseI AT'TA_AT
Cuts at: 160 1032 6418 160
Size: 872 5386 1606
Fragments arranged by size:
5386 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7864
AvaI C'yCGr_G
Cuts at: 1870 1943 5189 1870
Size: 73 3246 4545
Fragments arranged by size:
4545 3246 73
BamHI G'GATC_C
Cuts at: 5194 5561 5194
Size: 367 7497
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2075 2166 5185 6640 618
Size: 325 217 627 288 91 3019 1455 1842
Fragments arranged by size:
3019 1842 1455 627 325 288 217 91
BanII G_rGCy'C
Cuts at: 1391 3162 4247 5183 1391
Size: 1771 1085 936 4072
Fragments arranged by size:
4072 1771 1085 936
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3414 4094 4160 928
Size: 971 1515 680 66 4632
Fragments arranged by size:
4632 1515 971 680 66
BbvCI CC'TCA_GC
Cuts at: 3390 3390
Size: 7864
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2048 2082 2161 2195 6055 6089 1903
Size: 34 111 34 79 34 3860 34 3678
Fragments arranged by size:
3860 3678 111 79 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2539 5028 5756 6966 2539
Size: 2489 728 1210 3437
Fragments arranged by size:
3437 2489 1210 728
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7864
BfrBI ATG'CAT
Cuts at: 4043 4043
Size: 7864
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6475 136
Size: 107 122 71 631 605 4803 1525
Fragments arranged by size:
4803 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2725 4342 7859 2725
Size: 1617 3517 2730
Fragments arranged by size:
3517 2730 1617
BlpI GC'TnA_GC
Cuts at: 3126 5281 3126
Size: 2155 5709
Bme1580I G_kGCm'C
Cuts at: 1790 2078 5925 7081 1790
Size: 288 3847 1156 2573
Fragments arranged by size:
3847 2573 1156 288
BmgBI CAC'GTC
Cuts at: 1318 3903 1318
Size: 2585 5279
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5595 6553 442
Size: 416 53 83 399 24 4178 958 1753
Fragments arranged by size:
4178 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3042 1056
Size: 1986 5878
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2210 2460 6524 1239
Size: 971 250 4064 2579
Fragments arranged by size:
4064 2579 971 250
Bpu10I CC'TnA_GC
Cuts at: 2471 3156 3390 2471
Size: 685 234 6945
Fragments arranged by size:
6945 685 234
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4866 4929 4973 5989 6854 7152 4866
Size: 63 44 1016 865 298 5578
Fragments arranged by size:
5578 1016 865 298 63 44
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6527 882
Size: 617 5028 2219
Fragments arranged by size:
5028 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2125 5655 493
Size: 711 921 3530 2702
Fragments arranged by size:
3530 2702 921 711
BsaBI GATnn'nnATC
Cuts at: 3219 7858 3219
Size: 4639 3225
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2215 5671 6053 6688
Size: 53 83 186 936 682 3456 382 635
Cuts at: 6688 275
Size: 1451
Fragments arranged by size:
3456 1451 936 682 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6297 6695 6969 7116 7527 6297
Size: 398 274 147 411 6634
Fragments arranged by size:
6634 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2473 2503 3013 3043 5148
Size: 30 165 30 1070 30 510 30 2105
Cuts at: 5148 5178 1178
Size: 30 3864
Fragments arranged by size:
3864 2105 1070 510 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 5172 1186
Size: 3986 3878
BseYI C'CCAG_C
Cuts at: 7067 7563 7067
Size: 496 7368
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7291
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5314 6075 7103 1540
Size: 275 186 3313 761 1028 2301
Fragments arranged by size:
3313 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3847 5925 6010 7081 3847
Size: 2078 85 1071 4630
Fragments arranged by size:
4630 2078 1071 85
BsmI GAATG_Cn'
Cuts at: 5375 5468 5375
Size: 93 7771
BsmBI CGTCTCn'nnnn_
Cuts at: 3736 3736
Size: 7864
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2078 3162 3847 4247 5183 5925 6010
Size: 399 288 1084 685 400 936 742 85
Cuts at: 6010 7081 1391
Size: 1071 2174
Fragments arranged by size:
2174 1084 1071 936 742 685 400 399
288 85
BspEI T'CCGG_A
Cuts at: 7527 7527
Size: 7864
BspHI T'CATG_A
Cuts at: 2758 5753 7483 2758
Size: 2995 1730 3139
Fragments arranged by size:
3139 2995 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5219 844
Size: 4375 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5751 1394
Size: 429 3928 3507
Fragments arranged by size:
3928 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2092 6359 6533 65
Size: 1183 844 4267 174 1396
Fragments arranged by size:
4267 1396 1183 844 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6508 6695 1700
Size: 110 4698 187 2869
Fragments arranged by size:
4698 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2923 96
Size: 2827 5037
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7864
BssSI C'ACGA_G
Cuts at: 5924 6936 5924
Size: 1012 6852
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7864
BstBI TT'CG_AA
Cuts at: 6668 6668
Size: 7864
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7864
BstXI CCAn_nnnn'nTGG
Cuts at: 2566 4055 2566
Size: 1489 6375
BstYI r'GATC_y
Cuts at: 1972 2725 2773 4342 5194 5561 5946 5963 7415
Size: 753 48 1569 852 367 385 17 1452
Cuts at: 7415 7501 7513 7859 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1569 1452 852 753 385 367 346
86 48 17 12
Bsu36I CC'TnA_GG
Cuts at: 4287 4287
Size: 7864
BtgI C'CryG_G
Cuts at: 513 1065 4540 513
Size: 552 3475 3837
Fragments arranged by size:
3837 3475 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7125
Fragments arranged by size:
7125 683 56
BtsI GCAGTG_nn'
Cuts at: 3049 4942 5380 6174 6194 3049
Size: 1893 438 794 20 4719
Fragments arranged by size:
4719 1893 794 438 20
ClaI AT'CG_AT
Cuts at: 1554 2377 1554
Size: 823 7041
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7829
DraI TTT'AAA
Cuts at: 3447 5168 3447
Size: 1721 6143
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6871 809
Size: 507 5555 1802
Fragments arranged by size:
5555 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5311 6200 7829
Size: 54 1425 50 275 18 3481 889 1629
Cuts at: 7829 8
Size: 43
Fragments arranged by size:
3481 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5311 1537
Size: 275 3499 4090
Fragments arranged by size:
4090 3499 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2667 2934 3886 4549 5007 5794 1353
Size: 1314 267 952 663 458 787 3423
Fragments arranged by size:
3423 1314 952 787 663 458 267
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6451 6825 6971 387
Size: 1064 138 4862 374 146 1280
Fragments arranged by size:
4862 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 2460 3156 4844 5031 5124 5927
Size: 393 578 250 696 1688 187 93 803
Cuts at: 5927 6524 7311 7579 1239
Size: 597 787 268 1524
Fragments arranged by size:
1688 1524 803 787 696 597 578 393
268 250 187 93
EcoNI CCTnn'n_nnAGG
Cuts at: 2228 2228
Size: 7864
EcoO109I rG'GnC_Cy
Cuts at: 2221 2246 2221
Size: 25 7839
EcoRI G'AATT_C
Cuts at: 5173 5551 5173
Size: 378 7486
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2815 3505 4874 2815
Size: 690 1369 5805
Fragments arranged by size:
5805 1369 690
FspI TGC'GCA
Cuts at: 6370 6370
Size: 7864
HaeII r_GCGC'y
Cuts at: 1678 7011 1678
Size: 5333 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2420 2452 3167 3199 3863
Size: 32 135 32 848 32 715 32 664
Cuts at: 3863 3895 4090 4122 4685 4717 5006 5038 6505
Size: 32 195 32 563 32 289 32 1467
Cuts at: 6505 6537 6579 6611 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1467 848 715 664 563 289 195
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2554 3436 5208 5454 669
Size: 988 281 616 882 1772 246 3079
Fragments arranged by size:
3079 1772 988 882 616 281 246
HindIII A'AGCT_T
Cuts at: 748 4506 748
Size: 3758 4106
HpaI GTT'AAC
Cuts at: 5454 5454
Size: 7864
KpnI G_GTAC'C
Cuts at: 5189 5189
Size: 7864
MfeI C'AATT_G
Cuts at: 5463 5463
Size: 7864
MluI A'CGCG_T
Cuts at: 7740 7740
Size: 7864
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2391 2516 2658 4397 6978 7162 1305
Size: 1086 125 142 1739 2581 184 2007
Fragments arranged by size:
2581 2007 1739 1086 184 142 125
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6042
Fragments arranged by size:
6042 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3714 4026 4042 4371 4387 4663 4731
Size: 648 2548 312 16 329 16 276 68
Cuts at: 4731 5822 6181 6340 518
Size: 1091 359 159 2042
Fragments arranged by size:
2548 2042 1091 648 359 329 312 276
159 68 16 16
MspA1I CmG'CkG
Cuts at: 1606 2155 2164 2614 3389 5280 5957 7105 7350
Size: 549 9 450 775 1891 677 1148 245
Cuts at: 7350 1606
Size: 2120
Fragments arranged by size:
2120 1891 1148 775 677 549 450 245
9
NcoI C'CATG_G
Cuts at: 513 1065 4540 513
Size: 552 3475 3837
Fragments arranged by size:
3837 3475 552
NdeI CA'TA_TG
Cuts at: 387 3968 4011 4179 4800 387
Size: 3581 43 168 621 3451
Fragments arranged by size:
3581 3451 621 168 43
NheI G'CTAG_C
Cuts at: 1052 3038 1052
Size: 1986 5878
NotI GC'GGCC_GC
Cuts at: 5311 5311
Size: 7864
NsiI A_TGCA'T
Cuts at: 4045 4045
Size: 7864
NspI r_CATG'y
Cuts at: 1132 1229 4043 4047 4526 4677 4895 4921 5222
Size: 97 2814 4 479 151 218 26 301
Cuts at: 5222 6767 1132
Size: 1545 2229
Fragments arranged by size:
2814 2229 1545 479 301 218 151 97
26 4
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7864
PciI A'CATG_T
Cuts at: 4673 6763 4673
Size: 2090 5774
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7864
PmeI GTTT'AAAC
Cuts at: 5168 5168
Size: 7864
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5933 5965 7446 7478 5933
Size: 32 1481 32 6319
Fragments arranged by size:
6319 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2221 2246 2221
Size: 25 7839
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7864
PsiI TTA'TAA
Cuts at: 3758 5434 3758
Size: 1676 6188
PstI C_TGCA'G
Cuts at: 830 2205 3591 5216 830
Size: 1375 1386 1625 3478
Fragments arranged by size:
3478 1625 1386 1375
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7864
PvuII CAG'CTG
Cuts at: 2614 3389 2614
Size: 775 7089
SalI G'TCGA_C
Cuts at: 1936 5206 1936
Size: 3270 4594
SanDI GG'GwC_CC
Cuts at: 2246 2246
Size: 7864
SapI GCTCTTCn'nnn_
Cuts at: 4549 4549
Size: 7864
SbfI CC_TGCA'GG
Cuts at: 5216 5216
Size: 7864
ScaI AGT'ACT
Cuts at: 6112 7824 6112
Size: 1712 6152
SfcI C'TryA_G
Cuts at: 826 1045 2201 2496 2851 3587 4411 5212 6347
Size: 219 1156 295 355 736 824 801 1135
Cuts at: 6347 7028 7219 826
Size: 681 191 1471
Fragments arranged by size:
1471 1156 1135 824 801 736 681 355
295 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7864
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7864
SmaI CCC'GGG
Cuts at: 1872 5191 1872
Size: 3319 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4881 4944 4952 5968 6869 7131
Size: 197 926 2938 63 8 1016 901 262
Cuts at: 7131 820
Size: 1553
Fragments arranged by size:
2938 1553 1016 926 901 262 197 63
8
SnaBI TAC'GTA
Cuts at: 493 5655 493
Size: 5162 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7864
SphI G_CATG'C
Cuts at: 1132 4047 4921 5222 1132
Size: 2915 874 301 3774
Fragments arranged by size:
3774 2915 874 301
SspI AAT'ATT
Cuts at: 5 52 5788 5
Size: 47 5736 2081
Fragments arranged by size:
5736 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7864
StyI C'CwwG_G
Cuts at: 513 1065 4540 4873 5303 513
Size: 552 3475 333 430 3074
Fragments arranged by size:
3475 3074 552 430 333
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3008 5891 5908 6061 6246 6691 325
Size: 1255 1428 2883 17 153 185 445 1498
Fragments arranged by size:
2883 1498 1428 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2676 2833 2923
Size: 274 80 33 51 1443 699 157 90
Cuts at: 2923 2974 3007 3094 3825 4307 6110 7822 96
Size: 51 33 87 731 482 1803 1712 138
Fragments arranged by size:
1803 1712 1443 731 699 482 274 157
138 90 87 80 51 51 33 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2276 2308 4565 4597 4869 4901 7824 7856 2276
Size: 32 2257 32 272 32 2923 32 2284
Fragments arranged by size:
2923 2284 2257 272 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7864
XbaI T'CTAG_A
Cuts at: 5200 5200
Size: 7864
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7864
XmaI C'CCGG_G
Cuts at: 1870 5189 1870
Size: 3319 4545
XmnI GAAnn'nnTTC
Cuts at: 3411 4121 4382 5118 5993 3411
Size: 710 261 736 875 5282
Fragments arranged by size:
5282 875 736 710 261
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5672 276
Size: 53 83 186 936 4138 2468
Fragments arranged by size:
4138 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SexAI SfiI
SfoI SrfI SwaI XcmI