(Linear) (Six-base) MAPSORT of: KIEE4260.seq Check: 7181 from: 1 to: 3159 KIEE4260.orf With 182 enzymes: SgfI * March 22, 2012 17:38 .. AclI AA'CG_TT Cuts at: 0 570 1527 3159 Size: 570 957 1632 Fragments arranged by size: 1632 957 570 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1151 2839 3026 3119 3159 Size: 1151 1688 187 93 40 Fragments arranged by size: 1688 1151 187 93 40 AflIII A'CryG_T Cuts at: 0 2668 3159 Size: 2668 491 AleI CACnn'nnGTG Cuts at: 0 2021 3159 Size: 2021 1138 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2851 3159 Size: 2851 308 AlwNI CAG_nnn'CTG Cuts at: 0 1120 1174 3004 3159 Size: 1120 54 1830 155 Fragments arranged by size: 1830 1120 155 54 ApoI r'AATT_y Cuts at: 0 811 1948 2488 2596 2803 3159 Size: 811 1137 540 108 207 356 Fragments arranged by size: 1137 811 540 356 207 108 BanI G'GyrC_C Cuts at: 0 70 161 3159 Size: 70 91 2998 Fragments arranged by size: 2998 91 70 BanII G_rGCy'C Cuts at: 0 1157 2242 3159 Size: 1157 1085 917 Fragments arranged by size: 1157 1085 917 BbsI GAAGACnn'nnnn_ Cuts at: 0 1409 2089 2155 3159 Size: 1409 680 66 1004 Fragments arranged by size: 1409 1004 680 66 BbvCI CC'TCA_GC Cuts at: 0 1385 3159 Size: 1385 1774 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 43 77 156 190 3159 Size: 43 34 79 34 2969 Fragments arranged by size: 2969 79 43 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 534 3023 3159 Size: 534 2489 136 Fragments arranged by size: 2489 534 136 BfrBI ATG'CAT Cuts at: 0 2038 3159 Size: 2038 1121 BglII A'GATC_T Cuts at: 0 720 2337 3159 Size: 720 1617 822 Fragments arranged by size: 1617 822 720 BlpI GC'TnA_GC Cuts at: 0 1121 3159 Size: 1121 2038 Bme1580I G_kGCm'C Cuts at: 0 73 3159 Size: 73 3086 BmgBI CAC'GTC Cuts at: 0 1898 3159 Size: 1898 1261 BmtI G_CTAG'C Cuts at: 0 1037 3159 Size: 1037 2122 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 455 3159 Size: 205 250 2704 Fragments arranged by size: 2704 250 205 Bpu10I CC'TnA_GC Cuts at: 0 466 1151 1385 3159 Size: 466 685 234 1774 Fragments arranged by size: 1774 685 466 234 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2861 2924 2968 3159 Size: 2861 63 44 191 Fragments arranged by size: 2861 191 63 44 BsaAI yAC'GTr Cuts at: 0 120 3159 Size: 120 3039 BsaBI GATnn'nnATC Cuts at: 0 1214 3159 Size: 1214 1945 BsaHI Gr'CG_yC Cuts at: 0 210 3159 Size: 210 2949 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 468 498 1008 1038 3159 Size: 468 30 510 30 2121 Fragments arranged by size: 2121 510 468 30 30 BsiHKAI G_wGCw'C Cuts at: 0 1842 3159 Size: 1842 1317 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1731 3159 Size: 1731 1428 Bsp1286I G_dGCh'C Cuts at: 0 73 1157 1842 2242 3159 Size: 73 1084 685 400 917 Fragments arranged by size: 1084 917 685 400 73 BspHI T'CATG_A Cuts at: 0 753 3159 Size: 753 2406 BsrDI GCAATG_nn' Cuts at: 0 87 3159 Size: 87 3072 BsrGI T'GTAC_A Cuts at: 0 918 3159 Size: 918 2241 BstXI CCAn_nnnn'nTGG Cuts at: 0 561 2050 3159 Size: 561 1489 1109 Fragments arranged by size: 1489 1109 561 BstYI r'GATC_y Cuts at: 0 720 768 2337 3159 Size: 720 48 1569 822 Fragments arranged by size: 1569 822 720 48 Bsu36I CC'TnA_GG Cuts at: 0 2282 3159 Size: 2282 877 BtgI C'CryG_G Cuts at: 0 2535 3159 Size: 2535 624 BtsI GCAGTG_nn' Cuts at: 0 1044 2937 3159 Size: 1044 1893 222 Fragments arranged by size: 1893 1044 222 ClaI AT'CG_AT Cuts at: 0 372 3159 Size: 372 2787 DraI TTT'AAA Cuts at: 0 1442 3159 Size: 1442 1717 EarI CTCTTCn'nnn_ Cuts at: 0 662 929 1881 2544 3002 3159 Size: 662 267 952 663 458 157 Fragments arranged by size: 952 663 662 458 267 157 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 455 1151 2839 3026 3119 3159 Size: 205 250 696 1688 187 93 40 Fragments arranged by size: 1688 696 250 205 187 93 40 EcoNI CCTnn'n_nnAGG Cuts at: 0 223 3159 Size: 223 2936 EcoO109I rG'GnC_Cy Cuts at: 0 216 241 3159 Size: 216 25 2918 Fragments arranged by size: 2918 216 25 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 810 1500 2869 3159 Size: 810 690 1369 290 Fragments arranged by size: 1369 810 690 290 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 415 447 1162 1194 1858 1890 2085 2117 Size: 415 32 715 32 664 32 195 32 Cuts at: 2117 2680 2712 3001 3033 3159 Size: 563 32 289 32 126 Fragments arranged by size: 715 664 563 415 289 195 126 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 549 1431 3159 Size: 549 882 1728 Fragments arranged by size: 1728 882 549 HindIII A'AGCT_T Cuts at: 0 2501 3159 Size: 2501 658 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 386 511 653 2392 3159 Size: 386 125 142 1739 767 Fragments arranged by size: 1739 767 386 142 125 MslI CAynn'nnrTG Cuts at: 0 1709 2021 2037 2366 2382 2658 2726 3159 Size: 1709 312 16 329 16 276 68 433 Fragments arranged by size: 1709 433 329 312 276 68 16 16 MspA1I CmG'CkG Cuts at: 0 150 159 609 1384 3159 Size: 150 9 450 775 1775 Fragments arranged by size: 1775 775 450 150 9 NcoI C'CATG_G Cuts at: 0 2535 3159 Size: 2535 624 NdeI CA'TA_TG Cuts at: 0 1963 2006 2174 2795 3159 Size: 1963 43 168 621 364 Fragments arranged by size: 1963 621 364 168 43 NheI G'CTAG_C Cuts at: 0 1033 3159 Size: 1033 2126 NsiI A_TGCA'T Cuts at: 0 2040 3159 Size: 2040 1119 NspI r_CATG'y Cuts at: 0 2038 2042 2521 2672 2890 2916 3159 Size: 2038 4 479 151 218 26 243 Fragments arranged by size: 2038 479 243 218 151 26 4 PciI A'CATG_T Cuts at: 0 2668 3159 Size: 2668 491 PpuMI rG'GwC_Cy Cuts at: 0 216 241 3159 Size: 216 25 2918 Fragments arranged by size: 2918 216 25 PsiI TTA'TAA Cuts at: 0 1753 3159 Size: 1753 1406 PstI C_TGCA'G Cuts at: 0 200 1586 3159 Size: 200 1386 1573 Fragments arranged by size: 1573 1386 200 PvuII CAG'CTG Cuts at: 0 609 1384 3159 Size: 609 775 1775 Fragments arranged by size: 1775 775 609 SanDI GG'GwC_CC Cuts at: 0 241 3159 Size: 241 2918 SapI GCTCTTCn'nnn_ Cuts at: 0 2544 3159 Size: 2544 615 SfcI C'TryA_G Cuts at: 0 196 491 846 1582 2406 3159 Size: 196 295 355 736 824 753 Fragments arranged by size: 824 753 736 355 295 196 SmlI C'TyrA_G Cuts at: 0 2876 2939 2947 3159 Size: 2876 63 8 212 Fragments arranged by size: 2876 212 63 8 SphI G_CATG'C Cuts at: 0 2042 2916 3159 Size: 2042 874 243 Fragments arranged by size: 2042 874 243 StyI C'CwwG_G Cuts at: 0 2535 2868 3159 Size: 2535 333 291 Fragments arranged by size: 2535 333 291 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1003 3159 Size: 1003 2156 TatI w'GTAC_w Cuts at: 0 671 828 918 969 1002 1089 1820 2302 Size: 671 157 90 51 33 87 731 482 Cuts at: 2302 3159 Size: 857 Fragments arranged by size: 857 731 671 482 157 90 87 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 271 303 2560 2592 2864 2896 3159 Size: 271 32 2257 32 272 32 263 Fragments arranged by size: 2257 272 271 263 32 32 32 XmnI GAAnn'nnTTC Cuts at: 0 1406 2116 2377 3113 3159 Size: 1406 710 261 736 46 Fragments arranged by size: 1406 736 710 261 46 Enzymes that do cut: AclI AcuI AflIII AleI AlfI AlwNI ApoI BanI BanII BbsI BbvCI BcgI BciVI BfrBI BglII BlpI Bme1580I BmgBI BmtI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BsiHKAI BsmBI Bsp1286I BspHI BsrDI BsrGI BstXI BstYI Bsu36I BtgI BtsI ClaI DraI EarI Eco57MI EcoNI EcoO109I FalI Hin4I HincII HindIII MmeI MslI MspA1I NcoI NdeI NheI NsiI NspI PciI PpuMI PsiI PstI PvuII SanDI SapI SfcI SmlI SphI StyI TaqII TatI TstI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AfeI AflII AgeI AhdI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BclI BglI BmrI BplI BsaI BsaWI BseRI BseYI BsgI BsiEI BsiWI BsmI BspEI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI CspCI DraIII DrdI EaeI EagI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NgoMIV NotI NruI PacI PasI PflMI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaI ZraI