(Circular) (Six-base) MAPSORT of: pFN21AB3456.seq Check: 433 from: 1 to: 5782 pFN21AB3456 5782 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3592 278 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3125 1937 Size: 1188 4594 Acc65I G'GTAC_C Cuts at: 3103 3103 Size: 5782 AclI AA'CG_TT Cuts at: 1563 3909 4282 1563 Size: 2346 373 3063 Fragments arranged by size: 3063 2346 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3845 5229 5497 1632 Size: 2213 1384 268 1917 Fragments arranged by size: 2213 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5585 AflIII A'CryG_T Cuts at: 1203 4681 5658 1203 Size: 3478 977 1327 Fragments arranged by size: 3478 1327 977 AgeI A'CCGG_T Cuts at: 4613 4613 Size: 5782 AhdI GACnn_n'nnGTC Cuts at: 4511 4511 Size: 5782 AleI CACnn'nnGTG Cuts at: 1166 2699 1166 Size: 1533 4249 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5782 AlwNI CAG_nnn'CTG Cuts at: 3525 5097 3525 Size: 1572 4210 ApaLI G'TGCA_C Cuts at: 3839 4995 3839 Size: 1156 4626 ApoI r'AATT_y Cuts at: 1493 3091 3317 3469 5578 1493 Size: 1598 226 152 2109 1697 Fragments arranged by size: 2109 1697 1598 226 152 AseI AT'TA_AT Cuts at: 160 1032 4336 160 Size: 872 3304 1606 Fragments arranged by size: 3304 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 AvaI C'yCGr_G Cuts at: 1870 1943 2850 3107 1870 Size: 73 907 257 4545 Fragments arranged by size: 4545 907 257 73 BamHI G'GATC_C Cuts at: 3112 3479 3112 Size: 367 5415 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3103 4558 618 Size: 325 217 627 1316 1455 1842 Fragments arranged by size: 1842 1455 1316 627 325 217 BanII G_rGCy'C Cuts at: 1391 2263 2362 2467 3101 1391 Size: 872 99 105 634 4072 Fragments arranged by size: 4072 872 634 105 99 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2139 928 Size: 971 240 4571 Fragments arranged by size: 4571 971 240 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3973 4007 1903 Size: 34 50 34 1952 34 3678 Fragments arranged by size: 3678 1952 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3674 4884 3674 Size: 1210 4572 BclI T'GATC_A Cuts at: 1548 2418 1548 Size: 870 4912 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2407 3038 Size: 107 122 71 631 605 337 398 631 Cuts at: 3038 4393 136 Size: 1355 1525 Fragments arranged by size: 1525 1355 631 631 605 398 337 122 107 71 BglII A'GATC_T Cuts at: 5777 5777 Size: 5782 BlpI GC'TnA_GC Cuts at: 3199 3199 Size: 5782 Bme1580I G_kGCm'C Cuts at: 1790 2341 2482 2706 3843 4999 1790 Size: 551 141 224 1137 1156 2573 Fragments arranged by size: 2573 1156 1137 551 224 141 BmgBI CAC'GTC Cuts at: 1318 2528 1318 Size: 1210 4572 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3513 4471 442 Size: 416 53 83 399 24 2096 958 1753 Fragments arranged by size: 2096 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5782 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2062 2242 4442 1239 Size: 823 180 2200 2579 Fragments arranged by size: 2579 2200 823 180 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3907 4772 5070 3907 Size: 865 298 4619 Fragments arranged by size: 4619 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4445 882 Size: 617 2946 2219 Fragments arranged by size: 2946 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2548 3573 493 Size: 711 1344 1025 2702 Fragments arranged by size: 2702 1344 1025 711 BsaBI GATnn'nnATC Cuts at: 5776 5776 Size: 5782 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3589 3971 4606 275 Size: 53 83 186 936 2056 382 635 1451 Fragments arranged by size: 2056 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3009 3056 4215 4613 4887 5034 5445 3009 Size: 47 1159 398 274 147 411 3346 Fragments arranged by size: 3346 1159 411 398 274 147 47 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2056 2086 2894 2924 1178 Size: 30 165 30 653 30 808 30 4036 Fragments arranged by size: 4036 808 653 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2112 2662 1186 Size: 926 550 4306 Fragments arranged by size: 4306 926 550 BseYI C'CCAG_C Cuts at: 2575 4985 5481 2575 Size: 2410 496 2876 Fragments arranged by size: 2876 2410 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5209 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3232 3993 5021 1540 Size: 275 186 1231 761 1028 2301 Fragments arranged by size: 2301 1231 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2362 3843 3928 4999 2362 Size: 1481 85 1071 3145 Fragments arranged by size: 3145 1481 1071 85 BsmI GAATG_Cn' Cuts at: 2438 3293 3386 2438 Size: 855 93 4834 Fragments arranged by size: 4834 855 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2105 2105 Size: 5782 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2263 2341 2362 2467 2482 2706 3101 Size: 399 473 78 21 105 15 224 395 Cuts at: 3101 3843 3928 4999 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 473 399 395 224 105 85 78 21 15 BspEI T'CCGG_A Cuts at: 5445 5445 Size: 5782 BspHI T'CATG_A Cuts at: 3671 5401 3671 Size: 1730 4052 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2297 3137 844 Size: 1453 840 3489 Fragments arranged by size: 3489 1453 840 BsrBI CCG'CTC Cuts at: 1394 1823 2382 3669 1394 Size: 429 559 1287 3507 Fragments arranged by size: 3507 1287 559 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2421 4277 4451 65 Size: 1183 1173 1856 174 1396 Fragments arranged by size: 1856 1396 1183 1173 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2008 4426 4613 1700 Size: 110 198 2418 187 2869 Fragments arranged by size: 2869 2418 198 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5782 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5782 BssSI C'ACGA_G Cuts at: 2466 3842 4854 2466 Size: 1376 1012 3394 Fragments arranged by size: 3394 1376 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5782 BstBI TT'CG_AA Cuts at: 4586 4586 Size: 5782 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5782 BstXI CCAn_nnnn'nTGG Cuts at: 2434 2791 2434 Size: 357 5425 BstYI r'GATC_y Cuts at: 1972 3112 3479 3864 3881 5333 5419 5431 5777 Size: 1140 367 385 17 1452 86 12 346 Cuts at: 5777 1972 Size: 1977 Fragments arranged by size: 1977 1452 1140 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5230 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5043 Fragments arranged by size: 5043 683 56 BtsI GCAGTG_nn' Cuts at: 3298 4092 4112 3298 Size: 794 20 4968 Fragments arranged by size: 4968 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5782 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5747 DraI TTT'AAA Cuts at: 3086 3086 Size: 5782 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4789 809 Size: 507 3473 1802 Fragments arranged by size: 3473 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2985 3039 3229 Size: 54 1425 50 275 18 1155 54 190 Cuts at: 3229 4118 5747 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1155 889 275 190 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3229 1537 Size: 275 1417 4090 Fragments arranged by size: 4090 1417 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2819 3712 1353 Size: 1466 893 3423 Fragments arranged by size: 3423 1466 893 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4369 4743 4889 387 Size: 1064 138 2780 374 146 1280 Fragments arranged by size: 2780 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2360 2360 Size: 5782 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2062 2242 3845 4442 5229 5497 1239 Size: 393 430 180 1603 597 787 268 1524 Fragments arranged by size: 1603 1524 787 597 430 393 268 180 EcoO109I rG'GnC_Cy Cuts at: 2083 2083 Size: 5782 EcoRI G'AATT_C Cuts at: 3091 3469 3091 Size: 378 5404 EcoRV GAT'ATC Cuts at: 2032 2822 2032 Size: 790 4992 FspI TGC'GCA Cuts at: 2567 4288 2567 Size: 1721 4061 HaeII r_GCGC'y Cuts at: 1678 2072 4929 1678 Size: 394 2857 2531 Fragments arranged by size: 2857 2531 394 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2018 2050 4423 4455 4497 Size: 32 135 32 446 32 2373 32 42 Cuts at: 4497 4529 1373 Size: 32 2626 Fragments arranged by size: 2626 2373 446 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3126 3372 669 Size: 988 281 1188 246 3079 Fragments arranged by size: 3079 1188 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5782 HpaI GTT'AAC Cuts at: 3372 3372 Size: 5782 KpnI G_GTAC'C Cuts at: 3107 3107 Size: 5782 MfeI C'AATT_G Cuts at: 3381 3381 Size: 5782 MluI A'CGCG_T Cuts at: 5658 5658 Size: 5782 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2088 4896 5080 1305 Size: 783 2808 184 2007 Fragments arranged by size: 2808 2007 783 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3041 10 Size: 54 1425 343 1209 2751 Fragments arranged by size: 2751 1425 1209 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2237 2699 3740 4099 4258 518 Size: 648 1071 462 1041 359 159 2042 Fragments arranged by size: 2042 1071 1041 648 462 359 159 MspA1I CmG'CkG Cuts at: 1606 2579 2946 3198 3875 5023 5268 1606 Size: 973 367 252 677 1148 245 2120 Fragments arranged by size: 2120 1148 973 677 367 252 245 NaeI GCC'GGC Cuts at: 2010 2010 Size: 5782 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5230 NdeI CA'TA_TG Cuts at: 387 387 Size: 5782 NgoMIV G'CCGG_C Cuts at: 2008 2008 Size: 5782 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5782 NotI GC'GGCC_GC Cuts at: 3229 3229 Size: 5782 NspI r_CATG'y Cuts at: 1132 1229 2507 3140 4685 1132 Size: 97 1278 633 1545 2229 Fragments arranged by size: 2229 1545 1278 633 97 PasI CC'CwG_GG Cuts at: 1348 2341 1348 Size: 993 4789 PciI A'CATG_T Cuts at: 4681 4681 Size: 5782 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5782 PmeI GTTT'AAAC Cuts at: 3086 3086 Size: 5782 PmlI CAC'GTG Cuts at: 2548 2548 Size: 5782 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3851 3883 5364 5396 3851 Size: 32 1481 32 4237 Fragments arranged by size: 4237 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2083 2083 Size: 5782 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5782 PsiI TTA'TAA Cuts at: 3352 3352 Size: 5782 PstI C_TGCA'G Cuts at: 830 2025 2091 3134 830 Size: 1195 66 1043 3478 Fragments arranged by size: 3478 1195 1043 66 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5782 PvuII CAG'CTG Cuts at: 2579 2579 Size: 5782 SacI G_AGCT'C Cuts at: 2362 2362 Size: 5782 SalI G'TCGA_C Cuts at: 1936 3124 1936 Size: 1188 4594 SbfI CC_TGCA'GG Cuts at: 3134 3134 Size: 5782 ScaI AGT'ACT Cuts at: 4030 5742 4030 Size: 1712 4070 SfcI C'TryA_G Cuts at: 826 1045 2021 2087 3130 4265 4946 5137 826 Size: 219 976 66 1043 1135 681 191 1471 Fragments arranged by size: 1471 1135 1043 976 681 219 191 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5782 SmaI CCC'GGG Cuts at: 1872 2852 3109 1872 Size: 980 257 4545 Fragments arranged by size: 4545 980 257 SmlI C'TyrA_G Cuts at: 820 1017 1943 3886 4787 5049 820 Size: 197 926 1943 901 262 1553 Fragments arranged by size: 1943 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3573 493 Size: 3080 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5782 SphI G_CATG'C Cuts at: 1132 3140 1132 Size: 2008 3774 SspI AAT'ATT Cuts at: 5 52 3706 5 Size: 47 3654 2081 Fragments arranged by size: 3654 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5782 StyI C'CwwG_G Cuts at: 513 1065 2205 3024 3221 513 Size: 552 1140 819 197 3074 Fragments arranged by size: 3074 1140 819 552 197 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2589 2873 2984 3809 3826 3979 4164 Size: 1255 1009 284 111 825 17 153 185 Cuts at: 4164 4609 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1009 825 445 284 185 153 111 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4028 5740 96 Size: 274 80 33 51 1443 2051 1712 138 Fragments arranged by size: 2051 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2266 2298 5742 5774 2266 Size: 32 3444 32 2274 Fragments arranged by size: 3444 2274 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5782 XbaI T'CTAG_A Cuts at: 3118 3118 Size: 5782 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5782 XmaI C'CCGG_G Cuts at: 1870 2850 3107 1870 Size: 980 257 4545 Fragments arranged by size: 4545 980 257 XmnI GAAnn'nnTTC Cuts at: 2727 3911 2727 Size: 1184 4598 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3590 276 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI