################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## AccIII AclI AcsI AflII AgeI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp700I AspI AviII AvrII BamHI BbeI BbsI BcgI BciVI BfrI BlnI BpuAI BsaBI BsaI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrGI BssHII Bst1107I BstZ17I Bsu36I ClaI CpoI DraI DrdI Eco81I EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KasI KspI MamI MfeI MluI MroI MunI NarI NheI NotI NruI NsiI PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SfoI SgrAI SnaBI SpeI SphI SspBI SspI SwaI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 1147 GACGTC GACGT^C Acc65I 1844 GGTACC G^GTACC AhdI 307 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 1796 AGCGCT AGC^GCT Asp718I 1844 GGTACC G^GTACC AspEI 307 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BclI 1625 TGATCA T^GATCA BfaI 1039 CTAG C^TAG BlpI 1549 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1549 GCTGAGC GC^T[ACGT]AGC BsaWI 338 TCCGGT [AT]^CCGG[AT] BspLU11I 1967 ACATGT A^CATGT BssSI 250 CTCGTG CACGAG(-5/-1) BstBI 22 TTCGAA TT^CGAA CelII 1549 GCTGAGC GC^T[ACGT]AGC DraIII 2175 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 307 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1618 GAAGAG CTCTTC(1/4) Eco47III 1796 AGCGCT AGC^GCT EcoNI 2386 CCTGAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 1625 TGATCA T^GATCA HaeII 1796 AGCGCT [GA]GCGC^[CT] KpnI 1844 GGTACC GGTAC^C Ksp632I 1618 GAAGAG CTCTTC(1/4) MaeI 1039 CTAG C^TAG MseI 2402 TTAA T^TAA NaeI 2233 GCCGGC GCC^GGC NdeI 1678 CATATG CA^TATG NgoMIV 2233 GCCGGC G^CCGGC NspV 22 TTCGAA TT^CGAA PaeR7I 1279 CTCGAG C^TCGAG PvuII 1288 CAGCTG CAG^CTG SapI 1618 GAAGAGC GCTCTTC(1/4) SbfI 1137 CCTGCAGG CCTGCA^GG SexAI 1652 ACCTGGT A^CC[AT]GGT SfiI 1096 GGCCAGGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 22 TTCGAA TT^CGAA SmaI 924 CCCGGG CCC^GGG SmlI 1279 CTCGAG C^T[CT][GA]AG Sse8387I 1137 CCTGCAGG CCTGCA^GG Tru9I 2402 TTAA T^TAA XhoI 1279 CTCGAG C^TCGAG XmaCI 924 CCCGGG C^CCGGG XmaI 924 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AccI 1174 GTCTAC GT^[AC][GT]AC 2038 GTCTAC AlwI 1413 GATCC GGATC(4/5) 1511 GGATC BglI 177 GCCCTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1097 GCCAGGGCGGC BglII 1382 AGATCT A^GATCT 2192 AGATCT BsiEI 1104 CGGCCG CG[GA][CT]^CG 1723 CGGCCG BsmBI 1449 CGTCTC CGTCTC(1/5) 1995 CGTCTC BstAPI 879 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1259 GCAAAGTCTGC EagI 1104 CGGCCG C^GGCCG 1723 CGGCCG EclXI 1104 CGGCCG C^GGCCG 1723 CGGCCG Eco52I 1104 CGGCCG C^GGCCG 1723 CGGCCG NcoI 436 CCATGG C^CATGG 1322 CCATGG StuI 1367 AGGCCT AGG^CCT 2522 AGGCCT TfiI 1071 GATTC G^A[AT]TC 1933 GAATC Tsp509I 953 AATT ^AATT 996 AATT XcmI 1211 CCAAGACACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1298 CCAGCAACTGCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbrPI BbvCI BbvI BcnI BmyI BpmI BsaAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XhoII