(Circular) (Six-base) MAPSORT of: pFN21AE4240.seq Check: 4161 from: 1 to: 7330 pFN21AE4240 7330 bp With 182 enzymes: SgfI * March 28, 2012 13:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2722 3734 3830 2722 Size: 1012 96 6222 Fragments arranged by size: 6222 1012 96 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3156 5140 278 Size: 53 83 186 936 1620 1984 2468 Fragments arranged by size: 2468 1984 1620 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3180 4044 4673 1937 Size: 1243 864 629 4594 Fragments arranged by size: 4594 1243 864 629 Acc65I G'GTAC_C Cuts at: 3849 4651 3849 Size: 802 6528 AclI AA'CG_TT Cuts at: 1563 5457 5830 1563 Size: 3894 373 3063 Fragments arranged by size: 3894 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2503 3874 5393 6777 7045 1632 Size: 871 1371 1519 1384 268 1917 Fragments arranged by size: 1917 1519 1384 1371 871 268 AfeI AGC'GCT Cuts at: 3803 3803 Size: 7330 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7133 AflIII A'CryG_T Cuts at: 1203 3972 4203 4272 6229 7206 1203 Size: 2769 231 69 1957 977 1327 Fragments arranged by size: 2769 1957 1327 977 231 69 AgeI A'CCGG_T Cuts at: 6161 6161 Size: 7330 AhdI GACnn_n'nnGTC Cuts at: 2317 6059 2317 Size: 3742 3588 AleI CACnn'nnGTG Cuts at: 1166 2756 1166 Size: 1590 5740 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7330 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3986 4018 3986 Size: 32 7298 AlwNI CAG_nnn'CTG Cuts at: 2100 2213 2329 2644 2680 2701 2890 3309 5073 Size: 113 116 315 36 21 189 419 1764 Cuts at: 5073 6645 2100 Size: 1572 2785 Fragments arranged by size: 2785 1764 1572 419 315 189 116 113 36 21 ApaI G_GGCC'C Cuts at: 2589 2784 2856 4418 4509 2589 Size: 195 72 1562 91 5410 Fragments arranged by size: 5410 1562 195 91 72 ApaLI G'TGCA_C Cuts at: 5387 6543 5387 Size: 1156 6174 ApoI r'AATT_y Cuts at: 1493 4639 4865 5017 7126 1493 Size: 3146 226 152 2109 1697 Fragments arranged by size: 3146 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5884 160 Size: 872 4852 1606 Fragments arranged by size: 4852 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7330 AvaI C'yCGr_G Cuts at: 1870 1943 2113 2929 3284 3395 4067 4655 1870 Size: 73 170 816 355 111 672 588 4545 Fragments arranged by size: 4545 816 672 588 355 170 111 73 BamHI G'GATC_C Cuts at: 4660 5027 4660 Size: 367 6963 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2013 2179 2834 2934 3849 Size: 325 217 627 226 166 655 100 915 Cuts at: 3849 4651 6106 618 Size: 802 1455 1842 Fragments arranged by size: 1842 1455 915 802 655 627 325 226 217 166 100 BanII G_rGCy'C Cuts at: 1391 2589 2784 2856 3129 3191 3357 3561 4418 Size: 1198 195 72 273 62 166 204 857 Cuts at: 4418 4509 4539 4649 1391 Size: 91 30 110 4072 Fragments arranged by size: 4072 1198 857 273 204 195 166 110 91 72 62 30 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6359 BbvCI CC'TCA_GC Cuts at: 2144 2625 2694 3422 3483 3753 2144 Size: 481 69 728 61 270 5721 Fragments arranged by size: 5721 728 481 270 69 61 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5521 5555 1903 Size: 34 3584 34 3678 Fragments arranged by size: 3678 3584 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5222 6432 5222 Size: 1210 6120 BclI T'GATC_A Cuts at: 1548 3630 1548 Size: 2082 5248 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2188 3108 5941 Size: 107 122 71 631 605 516 920 2833 Cuts at: 5941 136 Size: 1525 Fragments arranged by size: 2833 1525 920 631 605 516 122 107 71 BglII A'GATC_T Cuts at: 3387 4197 7325 3387 Size: 810 3128 3392 Fragments arranged by size: 3392 3128 810 BlpI GC'TnA_GC Cuts at: 3555 4747 3555 Size: 1192 6138 Bme1580I G_kGCm'C Cuts at: 1790 2184 2589 2784 2839 2856 2937 4418 4509 Size: 394 405 195 55 17 81 1481 91 Cuts at: 4509 5391 6547 1790 Size: 882 1156 2573 Fragments arranged by size: 2573 1481 1156 882 405 394 195 91 81 55 17 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7330 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2823 5061 6019 Size: 416 53 83 399 24 1406 2238 958 Cuts at: 6019 442 Size: 1753 Fragments arranged by size: 2238 1753 1406 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7330 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2416 2416 Size: 7330 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3143 3179 3191 3248 3988 4232 4503 5990 Size: 1904 36 12 57 740 244 271 1487 Cuts at: 5990 1239 Size: 2579 Fragments arranged by size: 2579 1904 1487 740 271 244 57 36 12 Bpu10I CC'TnA_GC Cuts at: 2144 2625 2694 3422 3483 3753 2144 Size: 481 69 728 61 270 5721 Fragments arranged by size: 5721 728 481 270 69 61 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5455 6320 6618 5455 Size: 865 298 6167 Fragments arranged by size: 6167 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5993 882 Size: 617 4494 2219 Fragments arranged by size: 4494 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2581 4102 4204 4275 5121 493 Size: 711 1377 1521 102 71 846 2702 Fragments arranged by size: 2702 1521 1377 846 711 102 71 BsaBI GATnn'nnATC Cuts at: 7324 7324 Size: 7330 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2357 3153 3716 4242 Size: 53 83 186 936 824 796 563 526 Cuts at: 4242 5137 5519 6154 275 Size: 895 382 635 1451 Fragments arranged by size: 1451 936 895 824 796 635 563 526 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2343 5763 6161 6435 6582 6993 2343 Size: 3420 398 274 147 411 2680 Fragments arranged by size: 3420 2680 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2275 2305 3173 3203 3479 Size: 30 165 30 872 30 868 30 276 Cuts at: 3479 3509 3986 4016 4476 4506 1178 Size: 30 477 30 460 30 4002 Fragments arranged by size: 4002 872 868 477 460 276 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3428 3500 4550 1186 Size: 2242 72 1050 3966 Fragments arranged by size: 3966 2242 1050 72 BseYI C'CCAG_C Cuts at: 2049 2505 2589 2808 3593 3798 4513 6533 7029 Size: 456 84 219 785 205 715 2020 496 Cuts at: 7029 2049 Size: 2350 Fragments arranged by size: 2350 2020 785 715 496 456 219 205 84 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2206 3367 3924 1163 Size: 573 470 1161 557 4569 Fragments arranged by size: 4569 1161 573 557 470 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3112 3731 4780 5541 6569 1540 Size: 275 186 1111 619 1049 761 1028 2301 Fragments arranged by size: 2301 1111 1049 1028 761 619 275 186 BsiHKAI G_wGCw'C Cuts at: 3129 3357 3561 5391 5476 6547 3129 Size: 228 204 1830 85 1071 3912 Fragments arranged by size: 3912 1830 1071 228 204 85 BsmI GAATG_Cn' Cuts at: 4841 4934 4841 Size: 93 7237 BsmBI CGTCTCn'nnnn_ Cuts at: 3460 4006 3460 Size: 546 6784 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2184 2589 2784 2839 2856 2937 3129 Size: 399 394 405 195 55 17 81 192 Cuts at: 3129 3191 3357 3561 4418 4509 4539 4649 5391 Size: 62 166 204 857 91 30 110 742 Cuts at: 5391 5476 6547 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 857 742 405 399 394 204 195 192 166 110 91 85 81 62 55 30 17 BspEI T'CCGG_A Cuts at: 6993 6993 Size: 7330 BspHI T'CATG_A Cuts at: 5219 6949 5219 Size: 1730 5600 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2722 3150 3609 3734 3830 4685 844 Size: 1878 428 459 125 96 855 3489 Fragments arranged by size: 3489 1878 855 459 428 125 96 BsrBI CCG'CTC Cuts at: 1394 1823 5217 1394 Size: 429 3394 3507 Fragments arranged by size: 3507 3394 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5825 5999 65 Size: 1183 4577 174 1396 Fragments arranged by size: 4577 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2120 2228 4238 5974 6161 1700 Size: 110 310 108 2010 1736 187 2869 Fragments arranged by size: 2869 2010 1736 310 187 110 108 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7330 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7330 BssSI C'ACGA_G Cuts at: 2255 5390 6402 2255 Size: 3135 1012 3183 Fragments arranged by size: 3183 3135 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2890 3270 794 Size: 2096 380 4854 Fragments arranged by size: 4854 2096 380 BstBI TT'CG_AA Cuts at: 2028 6134 2028 Size: 4106 3224 BstEII G'GTnAC_C Cuts at: 1184 2278 2446 2557 1184 Size: 1094 168 111 5957 Fragments arranged by size: 5957 1094 168 111 BstXI CCAn_nnnn'nTGG Cuts at: 2257 2758 2905 2257 Size: 501 147 6682 Fragments arranged by size: 6682 501 147 BstYI r'GATC_y Cuts at: 1972 3387 3417 4197 4660 5027 5412 5429 6881 Size: 1415 30 780 463 367 385 17 1452 Cuts at: 6881 6967 6979 7325 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1415 780 463 385 367 346 86 30 17 12 BtgI C'CryG_G Cuts at: 513 1065 2175 2441 2451 2875 3327 3567 513 Size: 552 1110 266 10 424 452 240 4276 Fragments arranged by size: 4276 1110 552 452 424 266 240 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3430 487 Size: 683 56 2204 4387 Fragments arranged by size: 4387 2204 683 56 BtsI GCAGTG_nn' Cuts at: 2110 2567 2714 3221 3350 4453 4504 4846 5640 Size: 457 147 507 129 1103 51 342 794 Cuts at: 5640 5660 2110 Size: 20 3780 Fragments arranged by size: 3780 1103 794 507 457 342 147 129 51 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7330 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7295 DraI TTT'AAA Cuts at: 4634 4634 Size: 7330 DraIII CAC_nnn'GTG Cuts at: 4185 4185 Size: 7330 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6337 809 Size: 507 5021 1802 Fragments arranged by size: 5021 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2259 2457 2703 Size: 54 1425 50 275 18 429 198 246 Cuts at: 2703 2767 3100 3109 3207 3234 3300 3324 3330 Size: 64 333 9 98 27 66 24 6 Cuts at: 3330 3366 3402 3564 3728 3813 4777 5666 7295 Size: 36 36 162 164 85 964 889 1629 Cuts at: 7295 8 Size: 43 Fragments arranged by size: 1629 1425 964 889 429 333 275 246 198 164 162 98 85 66 64 54 50 43 36 36 27 24 18 9 6 EagI C'GGCC_G Cuts at: 1537 1812 3109 3728 4777 1537 Size: 275 1297 619 1049 4090 Fragments arranged by size: 4090 1297 1049 619 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3618 5260 1353 Size: 2265 1642 3423 Fragments arranged by size: 3423 2265 1642 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2806 3563 5917 6291 6437 387 Size: 1064 138 1217 757 2354 374 146 1280 Fragments arranged by size: 2354 1280 1217 1064 757 374 146 138 EcoICRI GAG'CTC Cuts at: 3127 3355 3559 3127 Size: 228 204 6898 Fragments arranged by size: 6898 228 204 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2503 3143 3179 3191 3248 3874 3988 Size: 393 871 640 36 12 57 626 114 Cuts at: 3988 4232 4503 5393 5990 6777 7045 1239 Size: 244 271 890 597 787 268 1524 Fragments arranged by size: 1524 890 871 787 640 626 597 393 271 268 244 114 57 36 12 EcoNI CCTnn'n_nnAGG Cuts at: 4395 4395 Size: 7330 EcoO109I rG'GnC_Cy Cuts at: 2542 2585 2586 2677 2747 2780 2781 2853 3070 Size: 43 1 91 70 33 1 72 217 Cuts at: 3070 3526 3655 4505 2542 Size: 456 129 850 5367 Fragments arranged by size: 5367 850 456 217 129 91 72 70 43 33 1 1 EcoRI G'AATT_C Cuts at: 4639 5017 4639 Size: 378 6952 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3457 3457 Size: 7330 FspI TGC'GCA Cuts at: 5836 5836 Size: 7330 HaeII r_GCGC'y Cuts at: 1678 3805 6477 1678 Size: 2127 2672 2531 Fragments arranged by size: 2672 2531 2127 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2303 2335 2410 2442 4476 Size: 32 135 32 731 32 75 32 2034 Cuts at: 4476 4508 5971 6003 6045 6077 1373 Size: 32 1463 32 42 32 2626 Fragments arranged by size: 2626 2034 1463 731 135 75 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2074 2662 3616 4674 4920 669 Size: 988 281 136 588 954 1058 246 3079 Fragments arranged by size: 3079 1058 988 954 588 281 246 136 HindIII A'AGCT_T Cuts at: 748 748 Size: 7330 HpaI GTT'AAC Cuts at: 4920 4920 Size: 7330 KpnI G_GTAC'C Cuts at: 3853 4655 3853 Size: 802 6528 MfeI C'AATT_G Cuts at: 4929 4929 Size: 7330 MluI A'CGCG_T Cuts at: 7206 7206 Size: 7330 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3483 6444 6628 1305 Size: 2178 2961 184 2007 Fragments arranged by size: 2961 2178 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2261 3326 3368 10 Size: 54 1425 343 429 1065 42 3972 Fragments arranged by size: 3972 1425 1065 429 343 54 42 MslI CAynn'nnrTG Cuts at: 518 1166 2493 2555 2756 2903 3036 5288 5647 Size: 648 1327 62 201 147 133 2252 359 Cuts at: 5647 5806 518 Size: 159 2042 Fragments arranged by size: 2252 2042 1327 648 359 201 159 147 133 62 MspA1I CmG'CkG Cuts at: 1606 2170 2599 2756 3099 3295 4023 4322 4746 Size: 564 429 157 343 196 728 299 424 Cuts at: 4746 5423 6571 6816 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 728 677 564 429 424 343 299 245 196 157 NaeI GCC'GGC Cuts at: 4240 4240 Size: 7330 NcoI C'CATG_G Cuts at: 513 1065 2441 3327 513 Size: 552 1376 886 4516 Fragments arranged by size: 4516 1376 886 552 NdeI CA'TA_TG Cuts at: 387 3684 387 Size: 3297 4033 NgoMIV G'CCGG_C Cuts at: 4238 4238 Size: 7330 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7330 NotI GC'GGCC_GC Cuts at: 4777 4777 Size: 7330 NspI r_CATG'y Cuts at: 1132 1229 2668 3976 4573 4688 6233 1132 Size: 97 1439 1308 597 115 1545 2229 Fragments arranged by size: 2229 1545 1439 1308 597 115 97 PasI CC'CwG_GG Cuts at: 1348 2323 2379 1348 Size: 975 56 6299 Fragments arranged by size: 6299 975 56 PciI A'CATG_T Cuts at: 3972 6229 3972 Size: 2257 5073 PflMI CCAn_nnn'nTGG Cuts at: 1108 2329 2379 4131 4275 4551 1108 Size: 1221 50 1752 144 276 3887 Fragments arranged by size: 3887 1752 1221 276 144 50 PfoI T'CCnGG_A Cuts at: 3156 3156 Size: 7330 PmeI GTTT'AAAC Cuts at: 4634 4634 Size: 7330 PmlI CAC'GTG Cuts at: 2581 4102 4275 2581 Size: 1521 173 5636 Fragments arranged by size: 5636 1521 173 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3986 4018 5399 5431 6912 6944 3986 Size: 32 1381 32 1481 32 4372 Fragments arranged by size: 4372 1481 1381 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2747 3070 3526 3655 2747 Size: 323 456 129 6422 Fragments arranged by size: 6422 456 323 129 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7330 PsiI TTA'TAA Cuts at: 4900 4900 Size: 7330 PspOMI G'GGCC_C Cuts at: 2585 2780 2852 4414 4505 2585 Size: 195 72 1562 91 5410 Fragments arranged by size: 5410 1562 195 91 72 PstI C_TGCA'G Cuts at: 830 2105 2886 3056 3147 3387 3745 4177 4448 Size: 1275 781 170 91 240 358 432 271 Cuts at: 4448 4682 830 Size: 234 3478 Fragments arranged by size: 3478 1275 781 432 358 271 240 234 170 91 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7330 PvuII CAG'CTG Cuts at: 3295 3295 Size: 7330 SacI G_AGCT'C Cuts at: 3129 3357 3561 3129 Size: 228 204 6898 Fragments arranged by size: 6898 228 204 SalI G'TCGA_C Cuts at: 1936 4672 1936 Size: 2736 4594 SapI GCTCTTCn'nnn_ Cuts at: 3618 3618 Size: 7330 SbfI CC_TGCA'GG Cuts at: 3147 4682 3147 Size: 1535 5795 ScaI AGT'ACT Cuts at: 5578 7290 5578 Size: 1712 5618 SexAI A'CCwGG_T Cuts at: 3657 3657 Size: 7330 SfcI C'TryA_G Cuts at: 826 1045 2101 2205 2882 3052 3143 3383 3741 Size: 219 1056 104 677 170 91 240 358 Cuts at: 3741 4173 4444 4678 5813 6494 6685 826 Size: 432 271 234 1135 681 191 1471 Fragments arranged by size: 1471 1135 1056 681 677 432 358 271 240 234 219 191 170 104 91 SfiI GGCCn_nnn'nGGCC Cuts at: 3108 3108 Size: 7330 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7330 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7330 SmaI CCC'GGG Cuts at: 1872 2931 4657 1872 Size: 1059 1726 4545 Fragments arranged by size: 4545 1726 1059 SmlI C'TyrA_G Cuts at: 820 1017 1943 3284 5434 6335 6597 820 Size: 197 926 1341 2150 901 262 1553 Fragments arranged by size: 2150 1553 1341 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5121 493 Size: 4628 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7330 SphI G_CATG'C Cuts at: 1132 4688 1132 Size: 3556 3774 SspI AAT'ATT Cuts at: 5 52 5254 5 Size: 47 5202 2081 Fragments arranged by size: 5202 2081 47 StuI AGG'CCT Cuts at: 1517 3374 4529 1517 Size: 1857 1155 4318 Fragments arranged by size: 4318 1857 1155 StyI C'CwwG_G Cuts at: 513 1065 2286 2441 3327 3369 3432 3477 3855 Size: 552 1221 155 886 42 63 45 378 Cuts at: 3855 4155 4769 513 Size: 300 614 3074 Fragments arranged by size: 3074 1221 886 614 552 378 300 155 63 45 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2434 5357 5374 5527 5712 6157 325 Size: 1255 854 2923 17 153 185 445 1498 Fragments arranged by size: 2923 1498 1255 854 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4162 5576 7288 Size: 274 80 33 51 1443 2185 1414 1712 Cuts at: 7288 96 Size: 138 Fragments arranged by size: 2185 1712 1443 1414 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2275 2307 4065 4097 7290 7322 2275 Size: 32 1758 32 3193 32 2283 Fragments arranged by size: 3193 2283 1758 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7330 XbaI T'CTAG_A Cuts at: 4666 4666 Size: 7330 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3223 3310 3223 Size: 87 7243 XhoI C'TCGA_G Cuts at: 1943 3284 1943 Size: 1341 5989 XmaI C'CCGG_G Cuts at: 1870 2929 4655 1870 Size: 1059 1726 4545 Fragments arranged by size: 4545 1726 1059 XmnI GAAnn'nnTTC Cuts at: 5459 5459 Size: 7330 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3154 5138 276 Size: 53 83 186 936 1620 1984 2468 Fragments arranged by size: 2468 1984 1620 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SfoI SrfI SwaI