(Linear) (Six-base) MAPSORT of: KIEE4240.seq Check: 9488 from: 1 to: 2625 KIEE4240.orf With 182 enzymes: SgfI * March 22, 2012 17:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 717 1729 1825 2625 Size: 717 1012 96 800 Fragments arranged by size: 1012 800 717 96 AatII G_ACGT'C Cuts at: 0 1151 2625 Size: 1151 1474 AccI GT'mk_AC Cuts at: 0 1175 2039 2625 Size: 1175 864 586 Fragments arranged by size: 1175 864 586 Acc65I G'GTAC_C Cuts at: 0 1844 2625 Size: 1844 781 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 498 1869 2625 Size: 498 1371 756 Fragments arranged by size: 1371 756 498 AfeI AGC'GCT Cuts at: 0 1798 2625 Size: 1798 827 AflIII A'CryG_T Cuts at: 0 1967 2198 2267 2625 Size: 1967 231 69 358 Fragments arranged by size: 1967 358 231 69 AhdI GACnn_n'nnGTC Cuts at: 0 312 2625 Size: 312 2313 AleI CACnn'nnGTG Cuts at: 0 751 2625 Size: 751 1874 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1981 2013 2625 Size: 1981 32 612 Fragments arranged by size: 1981 612 32 AlwNI CAG_nnn'CTG Cuts at: 0 95 208 324 639 675 696 885 1304 Size: 95 113 116 315 36 21 189 419 Cuts at: 1304 2625 Size: 1321 Fragments arranged by size: 1321 419 315 189 116 113 95 36 21 ApaI G_GGCC'C Cuts at: 0 584 779 851 2413 2504 2625 Size: 584 195 72 1562 91 121 Fragments arranged by size: 1562 584 195 121 91 72 AvaI C'yCGr_G Cuts at: 0 108 924 1279 1390 2062 2625 Size: 108 816 355 111 672 563 Fragments arranged by size: 816 672 563 355 111 108 BanI G'GyrC_C Cuts at: 0 8 174 829 929 1844 2625 Size: 8 166 655 100 915 781 Fragments arranged by size: 915 781 655 166 100 8 BanII G_rGCy'C Cuts at: 0 584 779 851 1124 1186 1352 1556 2413 Size: 584 195 72 273 62 166 204 857 Cuts at: 2413 2504 2534 2625 Size: 91 30 91 Fragments arranged by size: 857 584 273 204 195 166 91 91 72 62 30 BbvCI CC'TCA_GC Cuts at: 0 139 620 689 1417 1478 1748 2625 Size: 139 481 69 728 61 270 877 Fragments arranged by size: 877 728 481 270 139 69 61 BclI T'GATC_A Cuts at: 0 1625 2625 Size: 1625 1000 BglI GCCn_nnn'nGGC Cuts at: 0 183 1103 2625 Size: 183 920 1522 Fragments arranged by size: 1522 920 183 BglII A'GATC_T Cuts at: 0 1382 2192 2625 Size: 1382 810 433 Fragments arranged by size: 1382 810 433 BlpI GC'TnA_GC Cuts at: 0 1550 2625 Size: 1550 1075 Bme1580I G_kGCm'C Cuts at: 0 179 584 779 834 851 932 2413 2504 Size: 179 405 195 55 17 81 1481 91 Cuts at: 2504 2625 Size: 121 Fragments arranged by size: 1481 405 195 179 121 91 81 55 17 BmrI ACTGGGnnnn_n' Cuts at: 0 818 2625 Size: 818 1807 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 411 2625 Size: 411 2214 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1138 1174 1186 1243 1983 2227 2498 2625 Size: 1138 36 12 57 740 244 271 127 Fragments arranged by size: 1138 740 271 244 127 57 36 12 Bpu10I CC'TnA_GC Cuts at: 0 139 620 689 1417 1478 1748 2625 Size: 139 481 69 728 61 270 877 Fragments arranged by size: 877 728 481 270 139 69 61 BsaAI yAC'GTr Cuts at: 0 576 2097 2199 2270 2625 Size: 576 1521 102 71 355 Fragments arranged by size: 1521 576 355 102 71 BsaHI Gr'CG_yC Cuts at: 0 352 1148 1711 2237 2625 Size: 352 796 563 526 388 Fragments arranged by size: 796 563 526 388 352 BsaWI w'CCGG_w Cuts at: 0 338 2625 Size: 338 2287 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 270 300 1168 1198 1474 1504 1981 2011 Size: 270 30 868 30 276 30 477 30 Cuts at: 2011 2471 2501 2625 Size: 460 30 124 Fragments arranged by size: 868 477 460 276 270 124 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1423 1495 2545 2625 Size: 1423 72 1050 80 Fragments arranged by size: 1423 1050 80 72 BseYI C'CCAG_C Cuts at: 0 44 500 584 803 1588 1793 2508 2625 Size: 44 456 84 219 785 205 715 117 Fragments arranged by size: 785 715 456 219 205 117 84 44 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 201 1362 1919 2625 Size: 201 1161 557 706 Fragments arranged by size: 1161 706 557 201 BsiEI CG_ry'CG Cuts at: 0 1107 1726 2625 Size: 1107 619 899 Fragments arranged by size: 1107 899 619 BsiHKAI G_wGCw'C Cuts at: 0 1124 1352 1556 2625 Size: 1124 228 204 1069 Fragments arranged by size: 1124 1069 228 204 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1455 2001 2625 Size: 1455 546 624 Fragments arranged by size: 1455 624 546 Bsp1286I G_dGCh'C Cuts at: 0 179 584 779 834 851 932 1124 1186 Size: 179 405 195 55 17 81 192 62 Cuts at: 1186 1352 1556 2413 2504 2534 2625 Size: 166 204 857 91 30 91 Fragments arranged by size: 857 405 204 195 192 179 166 91 91 81 62 55 30 17 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 717 1145 1604 1729 1825 2625 Size: 717 428 459 125 96 800 Fragments arranged by size: 800 717 459 428 125 96 BsrFI r'CCGG_y Cuts at: 0 115 223 2233 2625 Size: 115 108 2010 392 Fragments arranged by size: 2010 392 115 108 BssSI C'ACGA_G Cuts at: 0 250 2625 Size: 250 2375 BstAPI GCAn_nnn'nTGC Cuts at: 0 885 1265 2625 Size: 885 380 1360 Fragments arranged by size: 1360 885 380 BstBI TT'CG_AA Cuts at: 0 23 2625 Size: 23 2602 BstEII G'GTnAC_C Cuts at: 0 273 441 552 2625 Size: 273 168 111 2073 Fragments arranged by size: 2073 273 168 111 BstXI CCAn_nnnn'nTGG Cuts at: 0 252 753 900 2625 Size: 252 501 147 1725 Fragments arranged by size: 1725 501 252 147 BstYI r'GATC_y Cuts at: 0 1382 1412 2192 2625 Size: 1382 30 780 433 Fragments arranged by size: 1382 780 433 30 BtgI C'CryG_G Cuts at: 0 170 436 446 870 1322 1562 2625 Size: 170 266 10 424 452 240 1063 Fragments arranged by size: 1063 452 424 266 240 170 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1425 2625 Size: 1425 1200 BtsI GCAGTG_nn' Cuts at: 0 105 562 709 1216 1345 2448 2499 2625 Size: 105 457 147 507 129 1103 51 126 Fragments arranged by size: 1103 507 457 147 129 126 105 51 DraIII CAC_nnn'GTG Cuts at: 0 2180 2625 Size: 2180 445 EaeI y'GGCC_r Cuts at: 0 254 452 698 762 1095 1104 1202 1229 Size: 254 198 246 64 333 9 98 27 Cuts at: 1229 1295 1319 1325 1361 1397 1559 1723 1808 Size: 66 24 6 36 36 162 164 85 Cuts at: 1808 2625 Size: 817 Fragments arranged by size: 817 333 254 246 198 164 162 98 85 66 64 36 36 27 24 9 6 EagI C'GGCC_G Cuts at: 0 1104 1723 2625 Size: 1104 619 902 Fragments arranged by size: 1104 902 619 EarI CTCTTCn'nnn_ Cuts at: 0 1613 2625 Size: 1613 1012 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 801 1558 2625 Size: 801 757 1067 Fragments arranged by size: 1067 801 757 EcoICRI GAG'CTC Cuts at: 0 1122 1350 1554 2625 Size: 1122 228 204 1071 Fragments arranged by size: 1122 1071 228 204 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 498 1138 1174 1186 1243 1869 1983 2227 Size: 498 640 36 12 57 626 114 244 Cuts at: 2227 2498 2625 Size: 271 127 Fragments arranged by size: 640 626 498 271 244 127 114 57 36 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 2390 2625 Size: 2390 235 EcoO109I rG'GnC_Cy Cuts at: 0 537 580 581 672 742 775 776 848 Size: 537 43 1 91 70 33 1 72 Cuts at: 848 1065 1521 1650 2500 2625 Size: 217 456 129 850 125 Fragments arranged by size: 850 537 456 217 129 125 91 72 70 43 33 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1452 2625 Size: 1452 1173 HaeII r_GCGC'y Cuts at: 0 1800 2625 Size: 1800 825 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 298 330 405 437 2471 2503 2625 Size: 298 32 75 32 2034 32 122 Fragments arranged by size: 2034 298 122 75 32 32 32 HincII GTy'rAC Cuts at: 0 69 657 1611 2625 Size: 69 588 954 1014 Fragments arranged by size: 1014 954 588 69 KpnI G_GTAC'C Cuts at: 0 1848 2625 Size: 1848 777 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1478 2625 Size: 1478 1147 MscI TGG'CCA Cuts at: 0 256 1321 1363 2625 Size: 256 1065 42 1262 Fragments arranged by size: 1262 1065 256 42 MslI CAynn'nnrTG Cuts at: 0 488 550 751 898 1031 2625 Size: 488 62 201 147 133 1594 Fragments arranged by size: 1594 488 201 147 133 62 MspA1I CmG'CkG Cuts at: 0 165 594 751 1094 1290 2018 2317 2625 Size: 165 429 157 343 196 728 299 308 Fragments arranged by size: 728 429 343 308 299 196 165 157 NaeI GCC'GGC Cuts at: 0 2235 2625 Size: 2235 390 NcoI C'CATG_G Cuts at: 0 436 1322 2625 Size: 436 886 1303 Fragments arranged by size: 1303 886 436 NdeI CA'TA_TG Cuts at: 0 1679 2625 Size: 1679 946 NgoMIV G'CCGG_C Cuts at: 0 2233 2625 Size: 2233 392 NspI r_CATG'y Cuts at: 0 663 1971 2568 2625 Size: 663 1308 597 57 Fragments arranged by size: 1308 663 597 57 PasI CC'CwG_GG Cuts at: 0 318 374 2625 Size: 318 56 2251 Fragments arranged by size: 2251 318 56 PciI A'CATG_T Cuts at: 0 1967 2625 Size: 1967 658 PflMI CCAn_nnn'nTGG Cuts at: 0 324 374 2126 2270 2546 2625 Size: 324 50 1752 144 276 79 Fragments arranged by size: 1752 324 276 144 79 50 PfoI T'CCnGG_A Cuts at: 0 1151 2625 Size: 1151 1474 PmlI CAC'GTG Cuts at: 0 576 2097 2270 2625 Size: 576 1521 173 355 Fragments arranged by size: 1521 576 355 173 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1981 2013 2625 Size: 1981 32 612 Fragments arranged by size: 1981 612 32 PpuMI rG'GwC_Cy Cuts at: 0 742 1065 1521 1650 2625 Size: 742 323 456 129 975 Fragments arranged by size: 975 742 456 323 129 PspOMI G'GGCC_C Cuts at: 0 580 775 847 2409 2500 2625 Size: 580 195 72 1562 91 125 Fragments arranged by size: 1562 580 195 125 91 72 PstI C_TGCA'G Cuts at: 0 100 881 1051 1142 1382 1740 2172 2443 Size: 100 781 170 91 240 358 432 271 Cuts at: 2443 2625 Size: 182 Fragments arranged by size: 781 432 358 271 240 182 170 100 91 PvuII CAG'CTG Cuts at: 0 1290 2625 Size: 1290 1335 SacI G_AGCT'C Cuts at: 0 1124 1352 1556 2625 Size: 1124 228 204 1069 Fragments arranged by size: 1124 1069 228 204 SapI GCTCTTCn'nnn_ Cuts at: 0 1613 2625 Size: 1613 1012 SbfI CC_TGCA'GG Cuts at: 0 1142 2625 Size: 1142 1483 SexAI A'CCwGG_T Cuts at: 0 1652 2625 Size: 1652 973 SfcI C'TryA_G Cuts at: 0 96 200 877 1047 1138 1378 1736 2168 Size: 96 104 677 170 91 240 358 432 Cuts at: 2168 2439 2625 Size: 271 186 Fragments arranged by size: 677 432 358 271 240 186 170 104 96 91 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1103 2625 Size: 1103 1522 SmaI CCC'GGG Cuts at: 0 926 2625 Size: 926 1699 SmlI C'TyrA_G Cuts at: 0 1279 2625 Size: 1279 1346 StuI AGG'CCT Cuts at: 0 1369 2524 2625 Size: 1369 1155 101 Fragments arranged by size: 1369 1155 101 StyI C'CwwG_G Cuts at: 0 281 436 1322 1364 1427 1472 1850 2150 Size: 281 155 886 42 63 45 378 300 Cuts at: 2150 2625 Size: 475 Fragments arranged by size: 886 475 378 300 281 155 63 45 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 429 2625 Size: 429 2196 TatI w'GTAC_w Cuts at: 0 2157 2625 Size: 2157 468 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 270 302 2060 2092 2625 Size: 270 32 1758 32 533 Fragments arranged by size: 1758 533 270 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1218 1305 2625 Size: 1218 87 1320 Fragments arranged by size: 1320 1218 87 XhoI C'TCGA_G Cuts at: 0 1279 2625 Size: 1279 1346 XmaI C'CCGG_G Cuts at: 0 924 2625 Size: 924 1701 ZraI GAC'GTC Cuts at: 0 1149 2625 Size: 1149 1476 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AfeI AflIII AhdI AleI AloI AlwNI ApaI AvaI BanI BanII BbvCI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KpnI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI SapI SbfI SexAI SfcI SfiI SmaI SmlI StuI StyI TaqII TatI TstI XcmI XhoI XmaI ZraI Enzymes that do not cut: AclI AflII AgeI AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BcgI BciVI BfrBI BmgBI BmtI BpuEI BsaI BsaBI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraI DrdI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI MfeI MluI NarI NheI NotI NruI NsiI PacI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI ScaI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XmnI