Restriction map for
pFN21AB3428
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BciVI BclI BcnI BfrI BglI
BglII BlnI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tsp509I Tth111I TthHB8I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AlwNI 502 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 164 CCCGAG C^[CT]CG[GA]G
BanI 489 GGTGCC G^G[CT][GA]CC
BanII 246 GGGCTC G[GA]GC[CT]^C
BcgI 1232 CGAAGGACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 947 CTAG C^TAG
BlpI 933 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 933 GCTCAGC GC^T[ACGT]AGC
BsoBI 164 CCCGAG C^[CT]CG[GA]G
BsrBI 5 CCGCTC CCGCTC(-3/-3)
BsrDI 417 CATTGC GCAATG(2/0)
BstXI 800 CCATGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 933 GCTCAGC GC^T[ACGT]AGC
CfoI 82 GCGC GCG^C
EcoRV 742 GATATC GAT^ATC
HhaI 82 GCGC GCG^C
HinP1I 82 GCGC G^CGC
HindIII 1219 AAGCTT A^AGCTT
MaeI 947 CTAG C^TAG
MseI 269 TTAA T^TAA
MspA1I 718 CAGCTG C[AC]G^C[GT]G
PflMI 561 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1047 GACGGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 580 CTGCAG CTGCA^G
PvuII 718 CAGCTG CAG^CTG
SapI 1185 GCTCTTC GCTCTTC(1/4)
Tru9I 269 TTAA T^TAA
Tsp45I 1066 GTCAC ^GT[GC]AC
Van91I 561 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccII 11 CGCG CG^CG
83 CGCG
AspHI 298 GTGCTC G[AT]GC[AT]^C
1183 GTGCTC
AvaII 1053 GGTCC G^G[AT]CC
1236 GGACC
BalI 92 TGGCCA TGG^CCA
413 TGGCCA
BseRI 573 CTCCTC GAGGAG(10/8)
463 GAGGAG
BsiHKAI 298 GTGCTC G[AT]GC[AT]^C
1183 GTGCTC
BsmAI 842 GAGAC GTCTC(1/5)
1210 GTCTC
BstUI 11 CGCG CG^CG
83 CGCG
BstYI 179 AGATCC [GA]^GATC[CT]
656 GGATCT
DraII 1052 AGGTCCC [GA]G^G[ACGT]CC[CT]
1235 AGGACCT
EcoO109I 1052 AGGTCCC [GA]G^G[ACGT]CC[CT]
1235 AGGACCT
HapII 453 CCGG C^CGG
677 CCGG
HpaII 453 CCGG C^CGG
677 CCGG
MaeII 818 ACGT A^CGT
1173 ACGT
MaeIII 472 GTAAC ^GT[ACGT]AC
1066 GTCAC
MflI 179 AGATCC [GA]^GATC[CT]
656 GGATCT
MluNI 92 TGGCCA TGG^CCA
413 TGGCCA
MscI 92 TGGCCA TGG^CCA
413 TGGCCA
MslI 906 CATTGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1035 CACCATTATG
MspI 453 CCGG C^CGG
677 CCGG
MvnI 11 CGCG CG^CG
83 CGCG
PpuMI 1052 AGGTCCC [GA]G^G[AT]CC[CT]
1235 AGGACCT
SfcI 580 CTGCAG C^T[GA][CT]AG
715 CTACAG
XcmI 68 CCAACAGCGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
435 CCAGGGCTTGTGTGG
XhoII 179 AGATCC [GA]^GATC[CT]
656 GGATCT
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI AlwI BbsI
BbvI BmyI BpmI BpuAI BsaJI
BsgI BsiYI BslI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EaeI
EarI EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MboI
MboII MnlI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI TseI TspRI