(Circular) (Six-base) MAPSORT of: pFN21AB3428.seq Check: 822 from: 1 to: 5974 pFN21AB3428 5974 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3784 278 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3317 1937 Size: 1380 4594 Acc65I G'GTAC_C Cuts at: 3295 3295 Size: 5974 AclI AA'CG_TT Cuts at: 1563 4101 4474 1563 Size: 2538 373 3063 Fragments arranged by size: 3063 2538 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2561 2810 3242 4037 5421 5689 1632 Size: 929 249 432 795 1384 268 1917 Fragments arranged by size: 1917 1384 929 795 432 268 249 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5777 AflIII A'CryG_T Cuts at: 1203 4873 5850 1203 Size: 3670 977 1327 Fragments arranged by size: 3670 1327 977 AgeI A'CCGG_T Cuts at: 4805 4805 Size: 5974 AhdI GACnn_n'nnGTC Cuts at: 4703 4703 Size: 5974 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5974 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2067 2694 1184 Size: 883 627 4464 Fragments arranged by size: 4464 883 627 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2053 2085 2053 Size: 32 5942 AlwNI CAG_nnn'CTG Cuts at: 2512 3717 5289 2512 Size: 1205 1572 3197 Fragments arranged by size: 3197 1572 1205 ApaLI G'TGCA_C Cuts at: 4031 5187 4031 Size: 1156 4818 ApoI r'AATT_y Cuts at: 1493 3283 3509 3661 5770 1493 Size: 1790 226 152 2109 1697 Fragments arranged by size: 2109 1790 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4528 160 Size: 872 3496 1606 Fragments arranged by size: 3496 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 AvaI C'yCGr_G Cuts at: 1870 1943 2169 3299 1870 Size: 73 226 1130 4545 Fragments arranged by size: 4545 1130 226 73 BamHI G'GATC_C Cuts at: 3304 3671 3304 Size: 367 5607 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2494 3295 4750 618 Size: 325 217 627 707 801 1455 1842 Fragments arranged by size: 1842 1455 801 707 627 325 217 BanII G_rGCy'C Cuts at: 1391 2255 3293 1391 Size: 864 1038 4072 Fragments arranged by size: 4072 1038 864 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2216 3003 3056 928 Size: 971 317 787 53 3846 Fragments arranged by size: 3846 971 787 317 53 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3226 3260 4165 4199 1903 Size: 34 1289 34 905 34 3678 Fragments arranged by size: 3678 1289 905 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3866 5076 3866 Size: 1210 4764 BclI T'GATC_A Cuts at: 1548 1548 Size: 5974 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4585 136 Size: 107 122 71 631 605 2913 1525 Fragments arranged by size: 2913 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5969 5969 Size: 5974 BlpI GC'TnA_GC Cuts at: 2939 3391 2939 Size: 452 5522 Bme1580I G_kGCm'C Cuts at: 1790 4035 5191 1790 Size: 2245 1156 2573 Fragments arranged by size: 2573 2245 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5974 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3705 4663 442 Size: 416 53 83 399 24 2288 958 1753 Fragments arranged by size: 2288 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5974 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3232 3232 Size: 5974 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2357 2834 2941 4634 1239 Size: 1118 477 107 1693 2579 Fragments arranged by size: 2579 1693 1118 477 107 Bpu10I CC'TnA_GC Cuts at: 2216 2216 Size: 5974 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4099 4964 5262 4099 Size: 865 298 4811 Fragments arranged by size: 4811 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4637 882 Size: 617 3138 2219 Fragments arranged by size: 3138 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3765 493 Size: 711 2561 2702 Fragments arranged by size: 2702 2561 711 BsaBI GATnn'nnATC Cuts at: 5968 5968 Size: 5974 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3781 4163 4798 275 Size: 53 83 186 936 2248 382 635 1451 Fragments arranged by size: 2248 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4407 4805 5079 5226 5637 4407 Size: 398 274 147 411 4744 Fragments arranged by size: 4744 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2792 2822 2838 2868 1178 Size: 30 165 30 1389 30 16 30 4284 Fragments arranged by size: 4284 1389 165 30 30 30 30 16 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2483 2569 1186 Size: 1297 86 4591 Fragments arranged by size: 4591 1297 86 BseYI C'CCAG_C Cuts at: 2667 5177 5673 2667 Size: 2510 496 2968 Fragments arranged by size: 2968 2510 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2063 2351 2711 1163 Size: 573 327 288 360 4426 Fragments arranged by size: 4426 573 360 327 288 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3424 4185 5213 1540 Size: 275 186 1423 761 1028 2301 Fragments arranged by size: 2301 1423 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2307 3192 4035 4120 5191 2307 Size: 885 843 85 1071 3090 Fragments arranged by size: 3090 1071 885 843 85 BsmI GAATG_Cn' Cuts at: 3485 3578 3485 Size: 93 5881 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2255 2307 3192 3293 4035 4120 5191 Size: 399 465 52 885 101 742 85 1071 Cuts at: 5191 1391 Size: 2174 Fragments arranged by size: 2174 1071 885 742 465 399 101 85 52 BspEI T'CCGG_A Cuts at: 5637 5637 Size: 5974 BspHI T'CATG_A Cuts at: 3863 5593 3863 Size: 1730 4244 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2292 2787 3252 3329 844 Size: 1448 495 465 77 3489 Fragments arranged by size: 3489 1448 495 465 77 BsrBI CCG'CTC Cuts at: 1394 1823 2012 3861 1394 Size: 429 189 1849 3507 Fragments arranged by size: 3507 1849 429 189 BsrDI GCAATG_nn' Cuts at: 65 1248 2421 4469 4643 65 Size: 1183 1173 2048 174 1396 Fragments arranged by size: 2048 1396 1183 1173 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4618 4805 1700 Size: 110 2808 187 2869 Fragments arranged by size: 2869 2808 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5974 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5974 BssSI C'ACGA_G Cuts at: 4034 5046 4034 Size: 1012 4962 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5974 BstBI TT'CG_AA Cuts at: 4778 4778 Size: 5974 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5974 BstXI CCAn_nnnn'nTGG Cuts at: 2812 2812 Size: 5974 BstYI r'GATC_y Cuts at: 1972 2184 2661 3304 3671 4056 4073 5525 5611 Size: 212 477 643 367 385 17 1452 86 Cuts at: 5611 5623 5969 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 643 477 385 367 346 212 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4483 Fragments arranged by size: 4483 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5235 Fragments arranged by size: 5235 683 56 BtsI GCAGTG_nn' Cuts at: 2110 3490 4284 4304 2110 Size: 1380 794 20 3780 Fragments arranged by size: 3780 1380 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5974 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5939 DraI TTT'AAA Cuts at: 3278 3278 Size: 5974 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4981 809 Size: 507 3665 1802 Fragments arranged by size: 3665 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2097 2418 Size: 54 1425 50 275 18 177 90 321 Cuts at: 2418 2459 3421 4310 5939 8 Size: 41 962 889 1629 43 Fragments arranged by size: 1629 1425 962 889 321 275 177 90 54 50 43 41 18 EagI C'GGCC_G Cuts at: 1537 1812 3421 1537 Size: 275 1609 4090 Fragments arranged by size: 4090 1609 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2300 2606 3197 3904 1353 Size: 947 306 591 707 3423 Fragments arranged by size: 3423 947 707 591 306 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4561 4935 5081 387 Size: 1064 138 2972 374 146 1280 Fragments arranged by size: 2972 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2357 2561 2810 2834 2941 3242 4037 Size: 393 725 204 249 24 107 301 795 Cuts at: 4037 4634 5421 5689 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 795 787 725 597 393 301 268 249 204 107 24 EcoO109I rG'GnC_Cy Cuts at: 3058 3241 3058 Size: 183 5791 EcoRI G'AATT_C Cuts at: 3283 3661 3283 Size: 378 5596 EcoRV GAT'ATC Cuts at: 2749 2749 Size: 5974 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2287 2287 Size: 5974 FspI TGC'GCA Cuts at: 4480 4480 Size: 5974 HaeII r_GCGC'y Cuts at: 1678 5121 1678 Size: 3443 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2146 2178 4615 4647 4689 Size: 32 135 32 574 32 2437 32 42 Cuts at: 4689 4721 1373 Size: 32 2626 Fragments arranged by size: 2626 2437 574 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3318 3564 669 Size: 988 281 1380 246 3079 Fragments arranged by size: 3079 1380 988 281 246 HindIII A'AGCT_T Cuts at: 748 3224 748 Size: 2476 3498 HpaI GTT'AAC Cuts at: 3564 3564 Size: 5974 KpnI G_GTAC'C Cuts at: 3299 3299 Size: 5974 MfeI C'AATT_G Cuts at: 3573 3573 Size: 5974 MluI A'CGCG_T Cuts at: 5850 5850 Size: 5974 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2097 5088 5272 1305 Size: 792 2991 184 2007 Fragments arranged by size: 2991 2007 792 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2099 2420 10 Size: 54 1425 343 267 321 3564 Fragments arranged by size: 3564 1425 343 321 267 54 MslI CAynn'nnrTG Cuts at: 518 1166 2915 3044 3932 4291 4450 518 Size: 648 1749 129 888 359 159 2042 Fragments arranged by size: 2042 1749 888 648 359 159 129 MspA1I CmG'CkG Cuts at: 1606 2725 3390 4067 5215 5460 1606 Size: 1119 665 677 1148 245 2120 Fragments arranged by size: 2120 1148 1119 677 665 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4483 Fragments arranged by size: 4483 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5974 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5974 NotI GC'GGCC_GC Cuts at: 3421 3421 Size: 5974 NspI r_CATG'y Cuts at: 1132 1229 3332 4877 1132 Size: 97 2103 1545 2229 Fragments arranged by size: 2229 2103 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5974 PciI A'CATG_T Cuts at: 4873 4873 Size: 5974 PflMI CCAn_nnn'nTGG Cuts at: 1108 2572 1108 Size: 1464 4510 PmeI GTTT'AAAC Cuts at: 3278 3278 Size: 5974 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2492 2524 4043 4075 5556 5588 2492 Size: 32 1519 32 1481 32 2878 Fragments arranged by size: 2878 1519 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3058 3241 3058 Size: 183 5791 PshAI GACnn'nnGTC Cuts at: 1613 3056 1613 Size: 1443 4531 PsiI TTA'TAA Cuts at: 3544 3544 Size: 5974 PstI C_TGCA'G Cuts at: 830 2589 3326 830 Size: 1759 737 3478 Fragments arranged by size: 3478 1759 737 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5974 PvuII CAG'CTG Cuts at: 2725 2725 Size: 5974 SalI G'TCGA_C Cuts at: 1936 3316 1936 Size: 1380 4594 SapI GCTCTTCn'nnn_ Cuts at: 3197 3197 Size: 5974 SbfI CC_TGCA'GG Cuts at: 3326 3326 Size: 5974 ScaI AGT'ACT Cuts at: 4222 5934 4222 Size: 1712 4262 SfcI C'TryA_G Cuts at: 826 1045 2585 2720 3322 4457 5138 5329 826 Size: 219 1540 135 602 1135 681 191 1471 Fragments arranged by size: 1540 1471 1135 681 602 219 191 135 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5974 SmaI CCC'GGG Cuts at: 1872 3301 1872 Size: 1429 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4078 4979 5241 820 Size: 197 926 2135 901 262 1553 Fragments arranged by size: 2135 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3765 493 Size: 3272 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5974 SphI G_CATG'C Cuts at: 1132 3332 1132 Size: 2200 3774 SspI AAT'ATT Cuts at: 5 52 3898 5 Size: 47 3846 2081 Fragments arranged by size: 3846 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5974 StyI C'CwwG_G Cuts at: 513 1065 2004 3413 513 Size: 552 939 1409 3074 Fragments arranged by size: 3074 1409 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2917 3163 4001 4018 4171 4356 4801 Size: 1255 1337 246 838 17 153 185 445 Cuts at: 4801 325 Size: 1498 Fragments arranged by size: 1498 1337 1255 838 445 246 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4220 5932 96 Size: 274 80 33 51 1443 2243 1712 138 Fragments arranged by size: 2243 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2838 2870 5934 5966 2838 Size: 32 3064 32 2846 Fragments arranged by size: 3064 2846 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5974 XbaI T'CTAG_A Cuts at: 3310 3310 Size: 5974 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2080 2447 2080 Size: 367 5607 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5974 XmaI C'CCGG_G Cuts at: 1870 3299 1870 Size: 1429 4545 XmnI GAAnn'nnTTC Cuts at: 4103 4103 Size: 5974 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3782 276 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI