Restriction map for
pFN21AB3428
(Linear) (Six-base) MAPSORT of: KIBB3428.seq Check: 8368 from: 1 to: 1269
KIBB3428.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 805 1237 1269
Size: 556 249 432 32
Fragments arranged by size:
556 432 249 32
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 62 689 1269
Size: 62 627 580
Fragments arranged by size:
627 580 62
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 48 80 1269
Size: 48 32 1189
Fragments arranged by size:
1189 48 32
AlwNI CAG_nnn'CTG
Cuts at: 0 507 1269
Size: 507 762
AvaI C'yCGr_G
Cuts at: 0 164 1269
Size: 164 1105
BanI G'GyrC_C
Cuts at: 0 489 1269
Size: 489 780
BanII G_rGCy'C
Cuts at: 0 250 1269
Size: 250 1019
BbsI GAAGACnn'nnnn_
Cuts at: 0 211 998 1051 1269
Size: 211 787 53 218
Fragments arranged by size:
787 218 211 53
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1221 1255 1269
Size: 1221 34 14
Fragments arranged by size:
1221 34 14
BlpI GC'TnA_GC
Cuts at: 0 934 1269
Size: 934 335
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1227 1269
Size: 1227 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 829 936 1269
Size: 352 477 107 333
Fragments arranged by size:
477 352 333 107
Bpu10I CC'TnA_GC
Cuts at: 0 211 1269
Size: 211 1058
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 787 817 833 863 1269
Size: 787 30 16 30 406
Fragments arranged by size:
787 406 30 30 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 478 564 1269
Size: 478 86 705
Fragments arranged by size:
705 478 86
BseYI C'CCAG_C
Cuts at: 0 662 1269
Size: 662 607
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 346 706 1269
Size: 58 288 360 563
Fragments arranged by size:
563 360 288 58
BsiHKAI G_wGCw'C
Cuts at: 0 302 1187 1269
Size: 302 885 82
Fragments arranged by size:
885 302 82
Bsp1286I G_dGCh'C
Cuts at: 0 250 302 1187 1269
Size: 250 52 885 82
Fragments arranged by size:
885 250 82 52
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 287 782 1247 1269
Size: 287 495 465 22
Fragments arranged by size:
495 465 287 22
BsrBI CCG'CTC
Cuts at: 0 7 1269
Size: 7 1262
BsrDI GCAATG_nn'
Cuts at: 0 416 1269
Size: 416 853
BstXI CCAn_nnnn'nTGG
Cuts at: 0 807 1269
Size: 807 462
BstYI r'GATC_y
Cuts at: 0 179 656 1269
Size: 179 477 613
Fragments arranged by size:
613 477 179
BtsI GCAGTG_nn'
Cuts at: 0 105 1269
Size: 105 1164
EaeI y'GGCC_r
Cuts at: 0 2 92 413 454 1269
Size: 2 90 321 41 815
Fragments arranged by size:
815 321 90 41 2
EarI CTCTTCn'nnn_
Cuts at: 0 295 601 1192 1269
Size: 295 306 591 77
Fragments arranged by size:
591 306 295 77
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 556 805 829 936 1237 1269
Size: 352 204 249 24 107 301 32
Fragments arranged by size:
352 301 249 204 107 32 24
EcoO109I rG'GnC_Cy
Cuts at: 0 1053 1236 1269
Size: 1053 183 33
Fragments arranged by size:
1053 183 33
EcoRV GAT'ATC
Cuts at: 0 744 1269
Size: 744 525
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 282 1269
Size: 282 987
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 141 173 1269
Size: 141 32 1096
Fragments arranged by size:
1096 141 32
HindIII A'AGCT_T
Cuts at: 0 1219 1269
Size: 1219 50
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 1269
Size: 92 1177
MscI TGG'CCA
Cuts at: 0 94 415 1269
Size: 94 321 854
Fragments arranged by size:
854 321 94
MslI CAynn'nnrTG
Cuts at: 0 910 1039 1269
Size: 910 129 230
Fragments arranged by size:
910 230 129
MspA1I CmG'CkG
Cuts at: 0 720 1269
Size: 720 549
PflMI CCAn_nnn'nTGG
Cuts at: 0 567 1269
Size: 567 702
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 487 519 1269
Size: 487 32 750
Fragments arranged by size:
750 487 32
PpuMI rG'GwC_Cy
Cuts at: 0 1053 1236 1269
Size: 1053 183 33
Fragments arranged by size:
1053 183 33
PshAI GACnn'nnGTC
Cuts at: 0 1051 1269
Size: 1051 218
PstI C_TGCA'G
Cuts at: 0 584 1269
Size: 584 685
PvuII CAG'CTG
Cuts at: 0 720 1269
Size: 720 549
SapI GCTCTTCn'nnn_
Cuts at: 0 1192 1269
Size: 1192 77
SfcI C'TryA_G
Cuts at: 0 580 715 1269
Size: 580 135 554
Fragments arranged by size:
580 554 135
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 912 1158 1269
Size: 912 246 111
Fragments arranged by size:
912 246 111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 833 865 1269
Size: 833 32 404
Fragments arranged by size:
833 404 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 75 442 1269
Size: 75 367 827
Fragments arranged by size:
827 367 75
Enzymes that do cut:
AcuI AlfI AloI AlwNI AvaI BanI BanII BbsI
BcgI BlpI BplI BpmI Bpu10I BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BstXI BstYI
BtsI EaeI EarI Eco57MI EcoO109I EcoRV FalI Hin4I
HindIII MmeI MscI MslI MspA1I PflMI PpiI PpuMI
PshAI PstI PvuII SapI SfcI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BclI
BfrBI BglI BglII Bme1580I BmgBI BmrI BmtI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TatI Tth111I XbaI
XhoI XmaI XmnI ZraI