Restriction map for
pFN21AB3393
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AocI
Aor51HI ApoI AscI AseI AsnI
AspI AvaI AvrII BamHI BbeI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
Hin1I HincII HindII HindIII HpaI
KasI KspI MaeI MfeI MluI
MroI MseI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI TaqI Tru9I Tsp509I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 128 GGTACC G^GTACC
AlwNI 196 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 128 GGTACC G^GTACC
BalI 339 TGGCCA TGG^CCA
BbrPI 708 CACGTG CAC^GTG
BbsI 268 GTCTTC GAAGAC(2/6)
BglI 611 GCCAGCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 571 GCTCAGC GC^T[ACGT]AGC
BpmI 427 CTGGAG CTGGAG(16/14)
Bpu1102I 571 GCTCAGC GC^T[ACGT]AGC
BpuAI 268 GTCTTC GAAGAC(2/6)
BsaAI 708 CACGTG [CT]AC^GT[GA]
BsaBI 831 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 492 CTGCAC GTGCAG(16/14)
BsmBI 430 GAGACG CGTCTC(1/5)
BspHI 779 TCATGA T^CATGA
BsrDI 84 CATTGC GCAATG(2/0)
BstAPI 195 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 629 CCATCCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 680 AGATCC [GA]^GATC[CT]
CelII 571 GCTCAGC GC^T[ACGT]AGC
HinfI 21 GATTC G^A[ACGT]TC
KpnI 128 GGTACC GGTAC^C
MamI 831 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 680 AGATCC [GA]^GATC[CT]
MluNI 339 TGGCCA TGG^CCA
MscI 339 TGGCCA TGG^CCA
NcoI 456 CCATGG C^CATGG
NspI 899 GCATGC [GA]CATG^[CT]
PmaCI 708 CACGTG CAC^GTG
PmlI 708 CACGTG CAC^GTG
PstI 193 CTGCAG CTGCA^G
PvuII 255 CAGCTG CAG^CTG
RcaI 779 TCATGA T^CATGA
SacI 569 GAGCTC GAGCT^C
SfaNI 392 GCATC GCATC(5/9)
SfcI 193 CTGCAG C^T[GA][CT]AG
SphI 899 GCATGC GCATG^C
TfiI 21 GATTC G^A[AT]TC
XhoII 680 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AhdI 352 GACTTTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
783 GACGCAGTGTC
Alw44I 379 GTGCAC G^TGCAC
442 GTGCAC
AlwI 681 GATCC GGATC(4/5)
764 GGATC
ApaI 245 GGGCCC GGGCC^C
289 GGGCCC
ApaLI 379 GTGCAC G^TGCAC
442 GTGCAC
AspEI 352 GACTTTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
783 GACGCAGTGTC
AviII 473 TGCGCA TGC^GCA
896 TGCGCA
BanI 128 GGTACC G^G[CT][GA]CC
211 GGCACC
BbvI 860 GCAGC GCAGC(8/12)
201 GCTGC
BcnI 234 CCGGG CC^[GC]GG
879 CCGGG
BsmAI 430 GAGAC GTCTC(1/5)
791 GTCTC
DdeI 110 CTAAG C^T[ACGT]AG
572 CTCAG
EaeI 218 CGGCCA [CT]^GGCC[GA]
339 TGGCCA
Eam1105I 352 GACTTTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
783 GACGCAGTGTC
FspI 473 TGCGCA TGC^GCA
896 TGCGCA
HaeII 419 AGCGCC [GA]GCGC^[CT]
588 GGCGCT
HapII 234 CCGG C^CGG
879 CCGG
HpaII 234 CCGG C^CGG
879 CCGG
MaeII 358 ACGT A^CGT
709 ACGT
MaeIII 262 GTGAC ^GT[ACGT]AC
398 GTCAC
MspA1I 255 CAGCTG C[AC]G^C[GT]G
659 CCGCTG
MspI 234 CCGG C^CGG
879 CCGG
NciI 234 CCGGG CC^[GC]GG
879 CCGGG
TseI 201 GCTGC G^C[AT]GC
860 GCAGC
Tsp45I 262 GTGAC ^GT[GC]AC
398 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AfaI AluI Asp700I
AspHI AvaII BanII BmyI BsaJI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DpnII
DraII DsaI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HgaI HhaI HinP1I HphI ItaI
Ksp632I MboI MboII MnlI MslI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PpuMI RsaI SapI Sau3AI
Sau96I ScrFI StyI TspRI XmnI