(Circular) (Six-base) MAPSORT of: pFN21AB3393.seq Check: 5608 from: 1 to: 5629 pFN21AB3393 5629 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2572 2572 Size: 5629 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3439 278 Size: 53 83 186 936 1903 2468 Fragments arranged by size: 2468 1903 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2972 1937 Size: 1035 4594 Acc65I G'GTAC_C Cuts at: 2133 2950 2133 Size: 817 4812 AclI AA'CG_TT Cuts at: 1563 3756 4129 1563 Size: 2193 373 3063 Fragments arranged by size: 3063 2193 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2201 2894 3692 5076 5344 1632 Size: 569 693 798 1384 268 1917 Fragments arranged by size: 1917 1384 798 693 569 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5432 AflIII A'CryG_T Cuts at: 1203 4528 5505 1203 Size: 3325 977 1327 Fragments arranged by size: 3325 1327 977 AgeI A'CCGG_T Cuts at: 4460 4460 Size: 5629 AhdI GACnn_n'nnGTC Cuts at: 2362 2793 4358 2362 Size: 431 1565 3633 Fragments arranged by size: 3633 1565 431 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5629 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5629 AlwNI CAG_nnn'CTG Cuts at: 2206 3372 4944 2206 Size: 1166 1572 2891 Fragments arranged by size: 2891 1572 1166 ApaI G_GGCC'C Cuts at: 2254 2298 2254 Size: 44 5585 ApaLI G'TGCA_C Cuts at: 2384 2447 3686 4842 2384 Size: 63 1239 1156 3171 Fragments arranged by size: 3171 1239 1156 63 ApoI r'AATT_y Cuts at: 1493 2938 3164 3316 5425 1493 Size: 1445 226 152 2109 1697 Fragments arranged by size: 2109 1697 1445 226 152 AseI AT'TA_AT Cuts at: 160 1032 4183 160 Size: 872 3151 1606 Fragments arranged by size: 3151 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5629 AvaI C'yCGr_G Cuts at: 1870 1943 2954 1870 Size: 73 1011 4545 Fragments arranged by size: 4545 1011 73 BamHI G'GATC_C Cuts at: 2959 3326 2959 Size: 367 5262 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2133 2216 2950 4405 618 Size: 325 217 627 346 83 734 1455 1842 Fragments arranged by size: 1842 1455 734 627 346 325 217 83 BanII G_rGCy'C Cuts at: 1391 2079 2254 2298 2578 2948 1391 Size: 688 175 44 280 370 4072 Fragments arranged by size: 4072 688 370 280 175 44 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2266 928 Size: 971 367 4291 Fragments arranged by size: 4291 971 367 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3820 3854 1903 Size: 34 1883 34 3678 Fragments arranged by size: 3678 1883 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3521 4731 3521 Size: 1210 4419 BclI T'GATC_A Cuts at: 1548 1548 Size: 5629 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2622 4240 136 Size: 107 122 71 631 605 950 1618 1525 Fragments arranged by size: 1618 1525 950 631 605 122 107 71 BglII A'GATC_T Cuts at: 5624 5624 Size: 5629 BlpI GC'TnA_GC Cuts at: 2577 3046 2577 Size: 469 5160 Bme1580I G_kGCm'C Cuts at: 1790 2219 2254 2298 2334 2388 2451 3690 4846 Size: 429 35 44 36 54 63 1239 1156 Cuts at: 4846 1790 Size: 2573 Fragments arranged by size: 2573 1239 1156 429 63 54 44 36 35 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5629 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2518 2795 3360 Size: 416 53 83 399 24 1101 277 565 Cuts at: 3360 4318 442 Size: 958 1753 Fragments arranged by size: 1753 1101 958 565 416 399 277 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5629 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2453 4289 1239 Size: 1214 1836 2579 Fragments arranged by size: 2579 1836 1214 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3754 4619 4917 3754 Size: 865 298 4466 Fragments arranged by size: 4466 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4292 882 Size: 617 2793 2219 Fragments arranged by size: 2793 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2715 3420 493 Size: 711 1511 705 2702 Fragments arranged by size: 2702 1511 711 705 BsaBI GATnn'nnATC Cuts at: 2840 5623 2840 Size: 2783 2846 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3436 3818 4453 275 Size: 53 83 186 936 1903 382 635 1451 Fragments arranged by size: 1903 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4062 4460 4734 4881 5292 4062 Size: 398 274 147 411 4399 Fragments arranged by size: 4399 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2039 2069 2441 2471 1178 Size: 30 165 30 636 30 372 30 4336 Fragments arranged by size: 4336 636 372 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5629 BseYI C'CCAG_C Cuts at: 2262 2666 4832 5328 2262 Size: 404 2166 496 2563 Fragments arranged by size: 2563 2166 496 404 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2482 1163 Size: 573 746 4310 Fragments arranged by size: 4310 746 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3079 3840 4868 1540 Size: 275 186 1078 761 1028 2301 Fragments arranged by size: 2301 1078 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2388 2451 2578 3690 3775 4846 2388 Size: 63 127 1112 85 1071 3171 Fragments arranged by size: 3171 1112 1071 127 85 63 BsmI GAATG_Cn' Cuts at: 3140 3233 3140 Size: 93 5536 BsmBI CGTCTCn'nnnn_ Cuts at: 2429 2429 Size: 5629 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2219 2254 2298 2334 2388 2451 Size: 399 289 140 35 44 36 54 63 Cuts at: 2451 2578 2948 3690 3775 4846 1391 Size: 127 370 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 370 289 140 127 85 63 54 44 36 35 BspEI T'CCGG_A Cuts at: 5292 5292 Size: 5629 BspHI T'CATG_A Cuts at: 2784 3518 5248 2784 Size: 734 1730 3165 Fragments arranged by size: 3165 1730 734 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2319 2572 2664 2984 844 Size: 1475 253 92 320 3489 Fragments arranged by size: 3489 1475 320 253 92 BsrBI CCG'CTC Cuts at: 1394 1823 3516 1394 Size: 429 1693 3507 Fragments arranged by size: 3507 1693 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2088 4124 4298 65 Size: 1183 840 2036 174 1396 Fragments arranged by size: 2036 1396 1183 840 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4273 4460 1700 Size: 110 2463 187 2869 Fragments arranged by size: 2869 2463 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5629 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5629 BssSI C'ACGA_G Cuts at: 3689 4701 3689 Size: 1012 4617 BstAPI GCAn_nnn'nTGC Cuts at: 794 2206 794 Size: 1412 4217 BstBI TT'CG_AA Cuts at: 4433 4433 Size: 5629 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5629 BstXI CCAn_nnnn'nTGG Cuts at: 2641 2641 Size: 5629 BstYI r'GATC_y Cuts at: 1972 2685 2959 3326 3711 3728 5180 5266 5278 Size: 713 274 367 385 17 1452 86 12 Cuts at: 5278 5624 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 713 385 367 346 274 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2211 2220 2461 513 Size: 552 1146 9 241 3681 Fragments arranged by size: 3681 1146 552 241 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4890 Fragments arranged by size: 4890 683 56 BtsI GCAGTG_nn' Cuts at: 2135 2798 3145 3939 3959 2135 Size: 663 347 794 20 3805 Fragments arranged by size: 3805 794 663 347 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5629 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5594 DraI TTT'AAA Cuts at: 2933 2933 Size: 5629 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4636 809 Size: 507 3320 1802 Fragments arranged by size: 3320 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2223 2344 3076 Size: 54 1425 50 275 18 393 121 732 Cuts at: 3076 3965 5594 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 732 393 275 121 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3076 1537 Size: 275 1264 4090 Fragments arranged by size: 4090 1264 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2114 2321 2447 2726 3559 1353 Size: 761 207 126 279 833 3423 Fragments arranged by size: 3423 833 761 279 207 126 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2144 4216 4590 4736 387 Size: 1064 138 555 2072 374 146 1280 Fragments arranged by size: 2072 1280 1064 555 374 146 138 EcoICRI GAG'CTC Cuts at: 2576 2576 Size: 5629 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2201 2453 2894 3692 4289 5076 5344 Size: 393 569 252 441 798 597 787 268 Cuts at: 5344 1239 Size: 1524 Fragments arranged by size: 1524 798 787 597 569 441 393 268 252 EcoO109I rG'GnC_Cy Cuts at: 2242 2250 2294 2295 2653 2763 2242 Size: 8 44 1 358 110 5108 Fragments arranged by size: 5108 358 110 44 8 1 EcoRI G'AATT_C Cuts at: 2938 3316 2938 Size: 378 5251 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2125 2725 2125 Size: 600 5029 FspI TGC'GCA Cuts at: 2480 2903 4135 2480 Size: 423 1232 3974 Fragments arranged by size: 3974 1232 423 HaeII r_GCGC'y Cuts at: 1678 2428 2597 4776 1678 Size: 750 169 2179 2531 Fragments arranged by size: 2531 2179 750 169 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2348 2380 2747 2779 2811 Size: 32 135 32 776 32 367 32 32 Cuts at: 2811 4270 4302 4344 4376 1373 Size: 1459 32 42 32 2626 Fragments arranged by size: 2626 1459 776 367 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2973 3219 669 Size: 988 281 1035 246 3079 Fragments arranged by size: 3079 1035 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5629 HpaI GTT'AAC Cuts at: 3219 3219 Size: 5629 KpnI G_GTAC'C Cuts at: 2137 2954 2137 Size: 817 4812 MfeI C'AATT_G Cuts at: 3228 3228 Size: 5629 MluI A'CGCG_T Cuts at: 5505 5505 Size: 5629 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4743 4927 1305 Size: 3438 184 2007 Fragments arranged by size: 3438 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2346 10 Size: 54 1425 343 514 3293 Fragments arranged by size: 3293 1425 514 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2352 2639 2834 3587 3946 4105 518 Size: 648 1186 287 195 753 359 159 2042 Fragments arranged by size: 2042 1186 753 648 359 287 195 159 MspA1I CmG'CkG Cuts at: 1606 2262 2666 3045 3722 4870 5115 1606 Size: 656 404 379 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 656 404 379 245 NcoI C'CATG_G Cuts at: 513 1065 2461 513 Size: 552 1396 3681 Fragments arranged by size: 3681 1396 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5629 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5629 NotI GC'GGCC_GC Cuts at: 3076 3076 Size: 5629 NspI r_CATG'y Cuts at: 1132 1229 2908 2987 4532 1132 Size: 97 1679 79 1545 2229 Fragments arranged by size: 2229 1679 1545 97 79 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5629 PciI A'CATG_T Cuts at: 4528 4528 Size: 5629 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5629 PfoI T'CCnGG_A Cuts at: 2238 2765 2883 2238 Size: 527 118 4984 Fragments arranged by size: 4984 527 118 PmeI GTTT'AAAC Cuts at: 2933 2933 Size: 5629 PmlI CAC'GTG Cuts at: 2715 2715 Size: 5629 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3698 3730 5211 5243 3698 Size: 32 1481 32 4084 Fragments arranged by size: 4084 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2242 2653 2763 2242 Size: 411 110 5108 Fragments arranged by size: 5108 411 110 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5629 PsiI TTA'TAA Cuts at: 3199 3199 Size: 5629 PspOMI G'GGCC_C Cuts at: 2250 2294 2250 Size: 44 5585 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2486 2518 2486 Size: 32 5597 PstI C_TGCA'G Cuts at: 830 2202 2981 830 Size: 1372 779 3478 Fragments arranged by size: 3478 1372 779 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5629 PvuII CAG'CTG Cuts at: 2262 2262 Size: 5629 SacI G_AGCT'C Cuts at: 2578 2578 Size: 5629 SalI G'TCGA_C Cuts at: 1936 2971 1936 Size: 1035 4594 SapI GCTCTTCn'nnn_ Cuts at: 2321 2447 2726 2321 Size: 126 279 5224 Fragments arranged by size: 5224 279 126 SbfI CC_TGCA'GG Cuts at: 2981 2981 Size: 5629 ScaI AGT'ACT Cuts at: 3877 5589 3877 Size: 1712 3917 SfcI C'TryA_G Cuts at: 826 1045 2198 2977 4112 4793 4984 826 Size: 219 1153 779 1135 681 191 1471 Fragments arranged by size: 1471 1153 1135 779 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5629 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5629 SmaI CCC'GGG Cuts at: 1872 2956 1872 Size: 1084 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3733 4634 4896 820 Size: 197 926 1790 901 262 1553 Fragments arranged by size: 1790 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3420 493 Size: 2927 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5629 SphI G_CATG'C Cuts at: 1132 2908 2987 1132 Size: 1776 79 3774 Fragments arranged by size: 3774 1776 79 SspI AAT'ATT Cuts at: 5 52 3553 5 Size: 47 3501 2081 Fragments arranged by size: 3501 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5629 StyI C'CwwG_G Cuts at: 513 1065 2166 2461 2850 3068 513 Size: 552 1101 295 389 218 3074 Fragments arranged by size: 3074 1101 552 389 295 218 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2254 2846 3656 3673 3826 4011 4456 Size: 1255 674 592 810 17 153 185 445 Cuts at: 4456 325 Size: 1498 Fragments arranged by size: 1498 1255 810 674 592 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2232 3875 5587 Size: 274 80 33 51 1443 255 1643 1712 Cuts at: 5587 96 Size: 138 Fragments arranged by size: 1712 1643 1443 274 255 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2039 2071 2220 2252 2441 2473 5589 5621 2039 Size: 32 149 32 189 32 3116 32 2047 Fragments arranged by size: 3116 2047 189 149 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5629 XbaI T'CTAG_A Cuts at: 2965 2965 Size: 5629 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5629 XmaI C'CCGG_G Cuts at: 1870 2954 1870 Size: 1084 4545 XmnI GAAnn'nnTTC Cuts at: 2108 2708 2879 3758 2108 Size: 600 171 879 3979 Fragments arranged by size: 3979 879 600 171 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3437 276 Size: 53 83 186 936 1903 2468 Fragments arranged by size: 2468 1903 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI