Restriction map for
pFN21AB3393
(Linear) (Six-base) MAPSORT of: KIBB3393.seq Check: 190 from: 1 to: 924
KIBB3393.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 567 924
Size: 567 357
Acc65I G'GTAC_C
Cuts at: 0 128 924
Size: 128 796
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 889 924
Size: 196 693 35
Fragments arranged by size:
693 196 35
AhdI GACnn_n'nnGTC
Cuts at: 0 357 788 924
Size: 357 431 136
Fragments arranged by size:
431 357 136
AlwNI CAG_nnn'CTG
Cuts at: 0 201 924
Size: 201 723
ApaI G_GGCC'C
Cuts at: 0 249 293 924
Size: 249 44 631
Fragments arranged by size:
631 249 44
ApaLI G'TGCA_C
Cuts at: 0 379 442 924
Size: 379 63 482
Fragments arranged by size:
482 379 63
BanI G'GyrC_C
Cuts at: 0 128 211 924
Size: 128 83 713
Fragments arranged by size:
713 128 83
BanII G_rGCy'C
Cuts at: 0 74 249 293 573 924
Size: 74 175 44 280 351
Fragments arranged by size:
351 280 175 74 44
BbsI GAAGACnn'nnnn_
Cuts at: 0 261 924
Size: 261 663
BglI GCCn_nnn'nGGC
Cuts at: 0 617 924
Size: 617 307
BlpI GC'TnA_GC
Cuts at: 0 572 924
Size: 572 352
Bme1580I G_kGCm'C
Cuts at: 0 214 249 293 329 383 446 924
Size: 214 35 44 36 54 63 478
Fragments arranged by size:
478 214 63 54 44 36 35
BmrI ACTGGGnnnn_n'
Cuts at: 0 513 790 924
Size: 513 277 134
Fragments arranged by size:
513 277 134
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 924
Size: 448 476
BsaAI yAC'GTr
Cuts at: 0 710 924
Size: 710 214
BsaBI GATnn'nnATC
Cuts at: 0 835 924
Size: 835 89
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 34 64 436 466 924
Size: 34 30 372 30 458
Fragments arranged by size:
458 372 34 30 30
BseYI C'CCAG_C
Cuts at: 0 257 661 924
Size: 257 404 263
Fragments arranged by size:
404 263 257
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 477 924
Size: 477 447
BsiHKAI G_wGCw'C
Cuts at: 0 383 446 573 924
Size: 383 63 127 351
Fragments arranged by size:
383 351 127 63
BsmBI CGTCTCn'nnnn_
Cuts at: 0 424 924
Size: 424 500
Bsp1286I G_dGCh'C
Cuts at: 0 74 214 249 293 329 383 446 573
Size: 74 140 35 44 36 54 63 127
Cuts at: 573 924
Size: 351
Fragments arranged by size:
351 140 127 74 63 54 44 36
35
BspHI T'CATG_A
Cuts at: 0 779 924
Size: 779 145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 314 567 659 924
Size: 314 253 92 265
Fragments arranged by size:
314 265 253 92
BsrDI GCAATG_nn'
Cuts at: 0 83 924
Size: 83 841
BstAPI GCAn_nnn'nTGC
Cuts at: 0 201 924
Size: 201 723
BstXI CCAn_nnnn'nTGG
Cuts at: 0 636 924
Size: 636 288
BstYI r'GATC_y
Cuts at: 0 680 924
Size: 680 244
BtgI C'CryG_G
Cuts at: 0 206 215 456 924
Size: 206 9 241 468
Fragments arranged by size:
468 241 206 9
BtsI GCAGTG_nn'
Cuts at: 0 130 793 924
Size: 130 663 131
Fragments arranged by size:
663 131 130
EaeI y'GGCC_r
Cuts at: 0 218 339 924
Size: 218 121 585
Fragments arranged by size:
585 218 121
EarI CTCTTCn'nnn_
Cuts at: 0 109 316 442 721 924
Size: 109 207 126 279 203
Fragments arranged by size:
279 207 203 126 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 139 924
Size: 139 785
EcoICRI GAG'CTC
Cuts at: 0 571 924
Size: 571 353
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 448 889 924
Size: 196 252 441 35
Fragments arranged by size:
441 252 196 35
EcoO109I rG'GnC_Cy
Cuts at: 0 237 245 289 290 648 758 924
Size: 237 8 44 1 358 110 166
Fragments arranged by size:
358 237 166 110 44 8 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 120 720 924
Size: 120 600 204
Fragments arranged by size:
600 204 120
FspI TGC'GCA
Cuts at: 0 475 898 924
Size: 475 423 26
Fragments arranged by size:
475 423 26
HaeII r_GCGC'y
Cuts at: 0 423 592 924
Size: 423 169 332
Fragments arranged by size:
423 332 169
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 343 375 742 774 806 924
Size: 343 32 367 32 32 118
Fragments arranged by size:
367 343 118 32 32 32
KpnI G_GTAC'C
Cuts at: 0 132 924
Size: 132 792
MscI TGG'CCA
Cuts at: 0 341 924
Size: 341 583
MslI CAynn'nnrTG
Cuts at: 0 347 634 829 924
Size: 347 287 195 95
Fragments arranged by size:
347 287 195 95
MspA1I CmG'CkG
Cuts at: 0 257 661 924
Size: 257 404 263
Fragments arranged by size:
404 263 257
NcoI C'CATG_G
Cuts at: 0 456 924
Size: 456 468
NspI r_CATG'y
Cuts at: 0 903 924
Size: 903 21
PfoI T'CCnGG_A
Cuts at: 0 233 760 878 924
Size: 233 527 118 46
Fragments arranged by size:
527 233 118 46
PmlI CAC'GTG
Cuts at: 0 710 924
Size: 710 214
PpuMI rG'GwC_Cy
Cuts at: 0 237 648 758 924
Size: 237 411 110 166
Fragments arranged by size:
411 237 166 110
PspOMI G'GGCC_C
Cuts at: 0 245 289 924
Size: 245 44 635
Fragments arranged by size:
635 245 44
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 481 513 924
Size: 481 32 411
Fragments arranged by size:
481 411 32
PstI C_TGCA'G
Cuts at: 0 197 924
Size: 197 727
PvuII CAG'CTG
Cuts at: 0 257 924
Size: 257 667
SacI G_AGCT'C
Cuts at: 0 573 924
Size: 573 351
SapI GCTCTTCn'nnn_
Cuts at: 0 316 442 721 924
Size: 316 126 279 203
Fragments arranged by size:
316 279 203 126
SfcI C'TryA_G
Cuts at: 0 193 924
Size: 193 731
SphI G_CATG'C
Cuts at: 0 903 924
Size: 903 21
StyI C'CwwG_G
Cuts at: 0 161 456 845 924
Size: 161 295 389 79
Fragments arranged by size:
389 295 161 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 249 841 924
Size: 249 592 83
Fragments arranged by size:
592 249 83
TatI w'GTAC_w
Cuts at: 0 227 924
Size: 227 697
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 34 66 215 247 436 468 924
Size: 34 32 149 32 189 32 456
Fragments arranged by size:
456 189 149 34 32 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 103 703 874 924
Size: 103 600 171 50
Fragments arranged by size:
600 171 103 50
Enzymes that do cut:
AarI Acc65I AcuI AhdI AlwNI ApaI ApaLI BanI
BanII BbsI BglI BlpI Bme1580I BmrI BpmI BsaAI
BsaBI BsaXI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrDI BstAPI BstXI BstYI BtgI BtsI EaeI
EarI EciI EcoICRI Eco57MI EcoO109I FalI FspI HaeII
Hin4I KpnI MscI MslI MspA1I NcoI NspI PfoI
PmlI PpuMI PspOMI PsrI PstI PvuII SacI SapI
SfcI SphI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AleI
AlfI AloI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglII BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaHI
BsaWI BseRI BsiEI BsiWI BsmI BspEI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EcoNI EcoRI
EcoRV FseI FspAI HincII HindIII HpaI KasI MfeI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspXI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI SspI StuI SwaI Tth111I
XbaI XcmI XhoI XmaI ZraI