(Circular) (Six-base) MAPSORT of: pFN21AB3388.seq Check: 2400 from: 1 to: 5314 pFN21AB3388 5314 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3124 278 Size: 53 83 186 936 1588 2468 Fragments arranged by size: 2468 1588 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2657 1937 Size: 720 4594 Acc65I G'GTAC_C Cuts at: 2635 2635 Size: 5314 AclI AA'CG_TT Cuts at: 1563 3441 3814 1563 Size: 1878 373 3063 Fragments arranged by size: 3063 1878 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2411 3377 4761 5029 1632 Size: 779 966 1384 268 1917 Fragments arranged by size: 1917 1384 966 779 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5117 AflIII A'CryG_T Cuts at: 1203 4213 5190 1203 Size: 3010 977 1327 Fragments arranged by size: 3010 1327 977 AgeI A'CCGG_T Cuts at: 4145 4145 Size: 5314 AhdI GACnn_n'nnGTC Cuts at: 2609 4043 2609 Size: 1434 3880 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5314 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5314 AlwNI CAG_nnn'CTG Cuts at: 2023 2086 3057 4629 2023 Size: 63 971 1572 2708 Fragments arranged by size: 2708 1572 971 63 ApaI G_GGCC'C Cuts at: 2230 2230 Size: 5314 ApaLI G'TGCA_C Cuts at: 3371 4527 3371 Size: 1156 4158 ApoI r'AATT_y Cuts at: 1493 2623 2849 3001 5110 1493 Size: 1130 226 152 2109 1697 Fragments arranged by size: 2109 1697 1130 226 152 AseI AT'TA_AT Cuts at: 160 1032 3868 160 Size: 872 2836 1606 Fragments arranged by size: 2836 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5314 AvaI C'yCGr_G Cuts at: 1870 1943 2205 2639 1870 Size: 73 262 434 4545 Fragments arranged by size: 4545 434 262 73 BamHI G'GATC_C Cuts at: 2644 3011 2644 Size: 367 4947 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2421 2635 4090 618 Size: 325 217 627 634 214 1455 1842 Fragments arranged by size: 1842 1455 634 627 325 217 214 BanII G_rGCy'C Cuts at: 1391 2230 2633 1391 Size: 839 403 4072 Fragments arranged by size: 4072 839 403 BbeI G_GCGC'C Cuts at: 2425 2425 Size: 5314 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4343 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3505 3539 1903 Size: 34 1568 34 3678 Fragments arranged by size: 3678 1568 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3206 4416 3206 Size: 1210 4104 BclI T'GATC_A Cuts at: 1548 1548 Size: 5314 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2072 3925 136 Size: 107 122 71 631 605 400 1853 1525 Fragments arranged by size: 1853 1525 631 605 400 122 107 71 BglII A'GATC_T Cuts at: 5309 5309 Size: 5314 BlpI GC'TnA_GC Cuts at: 2731 2731 Size: 5314 Bme1580I G_kGCm'C Cuts at: 1790 2230 3375 4531 1790 Size: 440 1145 1156 2573 Fragments arranged by size: 2573 1156 1145 440 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5314 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3045 4003 442 Size: 416 53 83 399 24 1628 958 1753 Fragments arranged by size: 1753 1628 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5314 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2063 2063 Size: 5314 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2492 3974 1239 Size: 1253 1482 2579 Fragments arranged by size: 2579 1482 1253 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2294 2360 2576 3439 4304 4602 2294 Size: 66 216 863 865 298 3006 Fragments arranged by size: 3006 865 863 298 216 66 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3977 882 Size: 617 2478 2219 Fragments arranged by size: 2478 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3105 493 Size: 711 1901 2702 Fragments arranged by size: 2702 1901 711 BsaBI GATnn'nnATC Cuts at: 5308 5308 Size: 5314 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2422 3121 3503 4138 Size: 53 83 186 936 889 699 382 635 Cuts at: 4138 275 Size: 1451 Fragments arranged by size: 1451 936 889 699 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2508 3747 4145 4419 4566 4977 2508 Size: 1239 398 274 147 411 2845 Fragments arranged by size: 2845 1239 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5089 Fragments arranged by size: 5089 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2489 1186 Size: 1303 4011 BseYI C'CCAG_C Cuts at: 2089 2231 4517 5013 2089 Size: 142 2286 496 2390 Fragments arranged by size: 2390 2286 496 142 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2036 1163 Size: 573 300 4441 Fragments arranged by size: 4441 573 300 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2764 3525 4553 1540 Size: 275 186 763 761 1028 2301 Fragments arranged by size: 2301 1028 763 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3375 3460 4531 3375 Size: 85 1071 4158 Fragments arranged by size: 4158 1071 85 BsiWI C'GTAC_G Cuts at: 2110 2110 Size: 5314 BsmI GAATG_Cn' Cuts at: 2825 2918 2825 Size: 93 5221 BsmBI CGTCTCn'nnnn_ Cuts at: 2312 2312 Size: 5314 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2230 2633 3375 3460 4531 1391 Size: 399 440 403 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 440 403 399 85 BspEI T'CCGG_A Cuts at: 4977 4977 Size: 5314 BspHI T'CATG_A Cuts at: 2526 3203 4933 2526 Size: 677 1730 2907 Fragments arranged by size: 2907 1730 677 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2008 2669 844 Size: 1164 661 3489 Fragments arranged by size: 3489 1164 661 BsrBI CCG'CTC Cuts at: 1394 1823 2297 3201 1394 Size: 429 474 904 3507 Fragments arranged by size: 3507 904 474 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3809 3983 65 Size: 1183 2561 174 1396 Fragments arranged by size: 2561 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3958 4145 1700 Size: 110 2148 187 2869 Fragments arranged by size: 2869 2148 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5314 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5314 BssSI C'ACGA_G Cuts at: 2432 3374 4386 2432 Size: 942 1012 3360 Fragments arranged by size: 3360 1012 942 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5314 BstBI TT'CG_AA Cuts at: 4118 4118 Size: 5314 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5314 BstYI r'GATC_y Cuts at: 1972 2499 2644 3011 3396 3413 4865 4951 4963 Size: 527 145 367 385 17 1452 86 12 Cuts at: 4963 5309 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 527 385 367 346 145 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2599 513 Size: 552 939 595 3228 Fragments arranged by size: 3228 939 595 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4575 Fragments arranged by size: 4575 683 56 BtsI GCAGTG_nn' Cuts at: 2463 2830 3624 3644 2463 Size: 367 794 20 4133 Fragments arranged by size: 4133 794 367 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5314 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5279 DraI TTT'AAA Cuts at: 2618 2618 Size: 5314 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4321 809 Size: 507 3005 1802 Fragments arranged by size: 3005 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2064 2761 3650 Size: 54 1425 50 275 18 234 697 889 Cuts at: 3650 5279 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 697 275 234 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2761 1537 Size: 275 949 4090 Fragments arranged by size: 4090 949 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2474 3244 1353 Size: 1121 770 3423 Fragments arranged by size: 3423 1121 770 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2263 3901 4275 4421 387 Size: 1064 138 435 239 1638 374 146 1280 Fragments arranged by size: 1638 1280 1064 435 374 239 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2411 2492 3377 3974 4761 5029 1239 Size: 393 779 81 885 597 787 268 1524 Fragments arranged by size: 1524 885 787 779 597 393 268 81 EcoNI CCTnn'n_nnAGG Cuts at: 2205 2205 Size: 5314 EcoO109I rG'GnC_Cy Cuts at: 2020 2210 2227 2587 2020 Size: 190 17 360 4747 Fragments arranged by size: 4747 360 190 17 EcoRI G'AATT_C Cuts at: 2623 3001 2623 Size: 378 4936 FspI TGC'GCA Cuts at: 3820 3820 Size: 5314 HaeII r_GCGC'y Cuts at: 1678 2425 4461 1678 Size: 747 2036 2531 Fragments arranged by size: 2531 2036 747 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2595 2627 3955 3987 4029 Size: 32 135 32 1023 32 1328 32 42 Cuts at: 4029 4061 1373 Size: 32 2626 Fragments arranged by size: 2626 1328 1023 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2658 2904 669 Size: 988 281 720 246 3079 Fragments arranged by size: 3079 988 720 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5314 HpaI GTT'AAC Cuts at: 2904 2904 Size: 5314 KasI G'GCGC_C Cuts at: 2421 2421 Size: 5314 KpnI G_GTAC'C Cuts at: 2639 2639 Size: 5314 MfeI C'AATT_G Cuts at: 2913 2913 Size: 5314 MluI A'CGCG_T Cuts at: 5190 5190 Size: 5314 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4428 4612 1305 Size: 3123 184 2007 Fragments arranged by size: 3123 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3492 Fragments arranged by size: 3492 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3272 3631 3790 518 Size: 648 2106 359 159 2042 Fragments arranged by size: 2106 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2730 3407 4555 4800 1606 Size: 1124 677 1148 245 2120 Fragments arranged by size: 2120 1148 1124 677 245 NarI GG'CG_CC Cuts at: 2422 2422 Size: 5314 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3823 Fragments arranged by size: 3823 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5314 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5314 NotI GC'GGCC_GC Cuts at: 2761 2761 Size: 5314 NspI r_CATG'y Cuts at: 1132 1229 2672 4217 1132 Size: 97 1443 1545 2229 Fragments arranged by size: 2229 1545 1443 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5314 PciI A'CATG_T Cuts at: 4213 4213 Size: 5314 PflMI CCAn_nnn'nTGG Cuts at: 1108 2050 1108 Size: 942 4372 PmeI GTTT'AAAC Cuts at: 2618 2618 Size: 5314 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3383 3415 4896 4928 3383 Size: 32 1481 32 3769 Fragments arranged by size: 3769 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2020 2587 2020 Size: 567 4747 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5314 PsiI TTA'TAA Cuts at: 2884 2884 Size: 5314 PspOMI G'GGCC_C Cuts at: 2226 2226 Size: 5314 PstI C_TGCA'G Cuts at: 830 2666 830 Size: 1836 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5314 RsrII CG'GwC_CG Cuts at: 2250 2250 Size: 5314 SalI G'TCGA_C Cuts at: 1936 2656 1936 Size: 720 4594 SbfI CC_TGCA'GG Cuts at: 2666 2666 Size: 5314 ScaI AGT'ACT Cuts at: 3562 5274 3562 Size: 1712 3602 SfcI C'TryA_G Cuts at: 826 1045 2146 2662 3797 4478 4669 826 Size: 219 1101 516 1135 681 191 1471 Fragments arranged by size: 1471 1135 1101 681 516 219 191 SfoI GGC'GCC Cuts at: 2423 2423 Size: 5314 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5314 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5314 SmaI CCC'GGG Cuts at: 1872 2641 1872 Size: 769 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2309 2375 2591 3418 4319 4581 Size: 197 926 366 66 216 827 901 262 Cuts at: 4581 820 Size: 1553 Fragments arranged by size: 1553 926 901 827 366 262 216 197 66 SnaBI TAC'GTA Cuts at: 493 3105 493 Size: 2612 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5314 SphI G_CATG'C Cuts at: 1132 2672 1132 Size: 1540 3774 SspI AAT'ATT Cuts at: 5 52 3238 5 Size: 47 3186 2081 Fragments arranged by size: 3186 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5314 StyI C'CwwG_G Cuts at: 513 1065 2004 2753 513 Size: 552 939 749 3074 Fragments arranged by size: 3074 939 749 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3341 3358 3511 3696 4141 325 Size: 1255 1761 17 153 185 445 1498 Fragments arranged by size: 1761 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3560 5272 96 Size: 274 80 33 51 1443 1583 1712 138 Fragments arranged by size: 1712 1583 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5274 5306 5274 Size: 32 5282 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5314 XbaI T'CTAG_A Cuts at: 2650 2650 Size: 5314 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5314 XmaI C'CCGG_G Cuts at: 1870 2639 1870 Size: 769 4545 XmnI GAAnn'nnTTC Cuts at: 3443 3443 Size: 5314 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3122 276 Size: 53 83 186 936 1588 2468 Fragments arranged by size: 2468 1588 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI RsrII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI